For the experiments, male C57BL/6 mice were arbitrarily allocated into four groups: (1) sham, (2) SSI, (3) sham+verapamil (SH+VP), and (4) SSI+verapamil (SSI+VP). The verapamil hydrochloride (5 mg/kg body weight, Sigma-Aldrich Co., St. Louis, USA) was diluted in sterile 0.9% NaCl saline (100 μL total volume/animal) and injected intraperitoneally (i.p.) two hours after CLP surgery (SSI+VP) or the sham operation (SH+VP). Untreated control (sham) and untreated septic mice (SSI) received an equivalent volume of saline. The survival rates were monitored every 12 hours for five days after surgery using 10 animals per group (sham, SSI, SH+VP, and SSI+VP, n = 10 per group).

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。