Immune infiltration analysis

The TIMER 2.0 database [14] (http://timer.cistrome.org/) is a comprehensive resource for the systematic analysis of immune infiltration in different cancer types based on data from TCGA. It integrates six state-of-the-art algorithms, including TIMER, xCell, MCP-counter, CIBERSORT, EPIC and quanTIseq. TISIDB [43] (http://cis.hku.hk/TISIDB/index.php) is a web portal for detecting tumor and immune system interactions. TISIDB integrates data from TCGA, PubMed literature, other public databases (UniProt, GO, DrugBank, etc.) and high-throughput sequencing analyses. The gene set variation analysis package was used to infer tumor-infiltrating lymphocyte levels in TISIDB. We further explored the relationship between ATP6AP1 and TIIC levels in BC and analyzed the surface markers of TIICs using the CellMaker [44] (http://biocc.hrbmu.edu.cn/CellMarker/) database.

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