All statistical analyses were conducted using IBM SPSS statistics software v.25 (IBM software, Chicago, USA). Tb, BW and HSP90, HSP70, HSF1, HSF3, IL6, IL8, TLR2, TLR4, CLDN1, CLDN5, CDH1, and OCLN mRNA levels were expressed as means±SD. However, VH, VW, VSA, CD, and VH-to-CD ratio were expressed as means±SEM. One-way analysis of variance followed by all-pairs Bonferroni test was used to compare different parameters in all treatment groups, while the Chi-squared test was also used to compare the hatchability rates between the different incubation groups. Parametric differences were considered statistically significant at p<0.05.