Mutant identification: Genomic DNA was extracted and samples were analyzed with HRMA [42]. Primers for Rad21l1 genotyping were the same as described in the rad21l1 mutant generation. Primers for Spo11 were Fwd: 5’-TCACAGCCAGGATGTTTTGA -3’ and Rev: 5’-CACCTGACATTGCAGCA-3’ with an annealing temperature of 61° C. Primers for Tp53 were Fwd: 5’-CTCCTGAGTCTCCAGAGTGATGA-3’ and Rev: 5’-ACTACATGTGCAATAGCAGCTGC-3’. Genomic DNA was extracted and samples were analyzed as described for rad21l1 mutants except that the reaction was done in 2 mM MgCl2 with an annealing temperature of 65° C. Two HRMA runs were required to confirm the three genotypes resulting from a tp53+/- incross; the first run distinguished heterozygous from homozygous (wild-type and mutant) samples. Homozygous samples were run again under the same conditions but spiked with wild-type DNA to differentiate wild-type and mutant samples.

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