The chemical structure of CMC-NH2 were characterized using 1H NMR (Bruker Avance III 400, Bruker, Karlsruhe, Germany). The sample was dissolved in D2O for measurement. The characterization of β-CD-CHO was confirmed by Fourier transform infrared spectroscopy (FTIR, Thermo Scientific, Wilmington, DE, USA), accumulating 36 scans from 400 to 4000 cm−1 with a resolution of 4 cm−1.The morphological characteristics of CK-Ngs were observed by scanning electron microscope (HITACH S4800, Hitachi, Tokyo, Japan). The particle size (DLS) and zeta potential were determined in a nano particle size and zeta potential analyzer (Zetasizer Nano ZS90, Malvern, UK). The qualitative assay of cellular uptake of CK-Ngs were done using confocal laser scanning microscopy (CLSM, A1R+N-SIM-S, Nikon, Tokyo, Japan).

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