The following antibodies were used: rabbit anti-53BP1 (Cat# GTX 112864, GeneTex, WB 1:1000), rabbit anti-ACCA antibody (Cell Signaling, Cat# 3662, RRID:AB_2219400, WB 1:5000), mouse anti-Actin antibody (Millipore, Cat# MAB1501, RRID:AB_2223041, WB 1:5000), rabbit anti-ACLY antibody (Sigma-Aldrich, Cat# HPA028758, RRID:AB_10603575, WB 1:2000), mouse anti-Aurora kinase antibody (BD Biosciences, Cat# 611082, RRID:AB_2227708, PLA 1:100), mouse-anti-BARD1 E-11 antibody (Santa Cruz, Cat# sc-74559, RRID:AB_2061237, WB 1:500), rabbit anti-BRCA1 20 antibody (Santa Cruz, Cat# sc-642, RRID:AB_630944, WB 1:500, IF 1:100, PLA 1:100), mouse anti-BRCA1 MS110 antibody (Abcam, Cat# ab16780, RRID:AB_2259338, WB 1:1000, IF 1:100, PLA 1:100), mouse-anti-BRCA1 D-9 antibody (Santa Cruz, Cat# sc-6964, RRID:AB_626761, IF 1:50), rabbit-anti-BRCA1 antibody (Millipore, Cat# 07-434, RRID:AB_2275035, WB 1:2000), rabbit-anti-BRCA1 antibody (Proteintech, Cat# 22363-1-AP, RRID:AB_2879090, WB 1:1000), rabbit anti-BRCA2 antibody (BioVision, Cat# 3675-30T, RRID:AB_2067764, WB 1:2000), rabbit anti-BRCC36 antibody (ProScience, Cat# 4311, WB 1:1000), rabbit anti-BRCC45 antibody (GeneTex, Cat# GTX105364, RRID:AB_1949757, WB 1:2000), mouse anti-Caldesmon antibody (BD Biosciences, Cat# 610660, WB 1:3000), mouse anti-alpha catenin antibody (Cell Signaling, Cat# 2131, WB 1:3000), mouse anti-gamma catenin antibody (Cell Signaling, Cat# 2309, WB 1:3000), rabbit anti-CAV1 antibody (BD Biosciences, Cat# 610060, WB 1:5000), mouse anti-cavin1 antibody (Abmart, China, 1:100 PLA), and rabbit anti-cavin1 antibody were raised as described previously and used for immunofluorescence (Bastiani et al., 2009), rabbit anti-cavin1 antibody (Sigma-Aldrich, Cat# AV36965, RRID:AB 1855947, WB 1:2000), mouse anti-cavin3 antibody (Novus, Cat# H00112464-MO4, PLA 1:200), rabbit anti-cavin3 antibody (Proteintech, Millennium Sciences, Pty, Ltd, Cat# 16250-1-AP, RRID:AB_2171897, WB 1:2000, IF 1:300, PLA 1:200), rabbit anti-CHD3 antibody (GeneTex, Sapphire Bioscience, Cat# GTX131779, RRID:AB_2886520, WB 1:500), rabbit anti-DDX21 antibody (Novus, Cat# NBP1-88310, RRID:AB_11027665, WB 1:2000), rabbit anti-EGFR Clone LA22 antibody (Millipore, Cat# 05-104, RRID:AB_11210086, WB 1:4000), mouse-anti-FANCD2 antibody (GeneTex, Cat# GTX116037, RRID:AB2036898, WB 1:500), mouse anti-Flotillin Clone 18 antibody (BD Biosciences, Cat# 610821, RRID:AB_398140, PLA 1:100), mouse anti-GFP antibody (Roche, Cat# 11814460001, RRID:AB_390913, WB 1:4000, PLA 1:300), rabbit anti-Histone H2A.X-Chip Grade (Abcam, Cat# ab20669, RRID:AB_445689, WB 1:1000), rabbit phospho-Histone H2A.X (Ser 139) (20E3) antibody (Cell Signaling Technology, Cat# 9718, RRID:AB_2118009, IF 1:500), rabbit phospho-Histone H2A.X CHIP Grade antibody (Abcam, Cat# ab2893, RRID:AB_303388, WB: 1:3000), rabbit anti-HLTF antibody (Proteintech, Cat# 14286-1-AP, WB 1:2000), rabbit anti-MDC1 antibody (Novus, Cat# 10056657SS, RRID:AB_838567, WB 1:100), sheep anti-Merit40 antibody (R&D Systems, Cat# AF6604SP, RRID:AB_10717577, WB 1:500), rabbit anti-PARP1 antibody (GeneTex, Cat# GTX112864, RRID:AB_11173565, WB 1:1000), mouse anti-PCNA antibody (Millipore, Cat# NA03T, RRID:AB_2160357, PLA 1:100), rabbit anti-PGK1 antibody (GeneTex, Cat# GTX107614, RRID:AB_2037666, WB 1:3000), rabbit anti-PKM antibody (GeneTex, Cat# GTX107977, RRID:AB_1951264, WB 1:3000), mouse anti-Rad51 antibody (Novus, Cat# 100-184, RRID:AB_350083, WB 1:1000), rabbit anti-RAP80 D1T6Q antibody (Cell Signaling Technology, Cat# 14466, RRID:AB_2798487, WB 1:1000, IF 1:100), rabbit anti-RNF168 antibody (GeneTex, Cat# GTX118147, RRID:AB_11169617, WB 1:1000), and mouse anti-Tubulin DM1A antibody (Abcam, Cat# ab7291, RRID:AB_2241126, WB 1:4000).

Secondary antibodies for immunofluorescence were Alexa Fluor 488 Goat anti-Rabbit IgG (H + L) (Thermo Fisher Scientific, Cat# A-11034, RRID:AB_141637, IF 1:500), Alexa Fluor 546 Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, Cat# A-11030, RRID:AB_2534089, IF 1:500), Alexa Fluor 594 Donkey anti-Rabbit IgG (H + L) (Thermo Fisher Scientific, Cat# A-21207, RRID:AB_141637, IF 1:500), and Alexa Fluor 594 Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, Cat# A-21203, RRID:AB_141633, IF 1:500). Secondary antibodies for western blotting were Goat anti-Mouse IgG (H + L) cross adsorbed secondary antibody, HRP (Thermo Fisher Scientific, Cat# G-21040, RRID:AB_2536527, WB 1:5000), Goat anti-Mouse IgG (H + L) cross adsorbed secondary antibody, HRP (Thermo Fisher Scientific, Cat# G-21234, RRID:AB_2536527, WB 1:5000), and Rabbit anti-Sheep IgG (H + L) (Abcam, Cat# ab97130, RRID:AB_2536530, WB 1:2000).

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



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