MCF7 cells, a human adenocarcinoma cell line with a low invasive phenotype (ATTC HBT-22, RRID:CVCL_0031), were subjected to STR profiling (QIMR Berghofer Cancer Research Institute). MDA-MB231 cells (ATCC HTB-26, RRID:CVCL_0062), a human adenocarcinoma cell line, and A431 cells (ATCC CRL-1555, RRID:CVCL_0037), HeLa cells (ATCC CRM-CCL2, RRID:CVCL_0030), and HeLa KO for cavin3 were cultured in DMEM supplemented with 10% (vol/vol) FBS, 100 units/ml penicillin, and 100 μg/ml streptomycin. All cell lines were routinely tested for mycoplasma. MCF7 cells were seeded at 1 × 106 cells and were transfected with 5 μg pEGFP DNA, pEGFP-cavin1, pEGFP-cavin2, pEGFP-cavin3, or pEGFP-CAV1 DNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. G418 (Sigma-Aldrich, Cat# 472788001) was used as a selection drug at 500 μg/ml.