A431 or MDA-MB231 cells were plated on coverslips at 70% confluency. Cells were either left untreated or were treated with 200 μM H2O2 for 30 min, 90% hypo-osmotic media for 10 min, or UV treatment for 2 min without media with a UV germicidal light source (UV-C 254 nm) and allowed to recover for 30 min in complete cell culture medium as previously described in McMahon et al., 2019. All cells were fixed and processed for cavin3 and BRCA1 or cavin3 and cavin1 using the PLA as described.