• 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011

现期刊物(2019)

卷册: 9, 期号: 1

癌症生物学

Evaluation of Anticancer activity of Silver Nanoparticles on the A549 Human Lung Carcinoma Cell Lines through Alamar Blue Assay 通过阿尔玛蓝显色法评估A549人肺癌细胞系上的银纳米粒子 作者:Nikita Sharma, Geeta Arya, R. Mankamna Kumari, Nidhi Gupta and Surendra Nimesh日期:01/05/2019,浏览量:218,Q&A: 0
Silver nanoparticles have been widely studied to possess antimicrobial as well as anticancer activity, and have found its applications in various fields including pharmaceutical industry, diagnostics, drug delivery, food industry, and others. For this purpose, several cell proliferation assays are widely used for the evaluation of anticancer ...
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发育生物学

Heterochronic Phenotype Analysis of Hypodermal Seam Cells in Caenorhabditis elegans 秀丽隐杆线虫下皮干细胞的异时表型分析 作者:Yon Ju Ji and Jiou Wang日期:01/05/2019,浏览量:174,Q&A: 0
Heterochrony refers to changes in the timing of developmental events, and it is precisely regulated in the organisms by the heterochronic genes such as C. elegans lin-4 and let-7. Mutations in these genes cause precocious or retarded development of certain cell lineages. With well-defined cell lineages, C. elegans ...
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Cartilage Induction from Mouse Mesenchymal Stem Cells in High-density Micromass Culture 高密度微团培养的小鼠骨髓间充质干细胞诱导分化生成的软骨 作者:Takashi Iezaki, Kazuya Fukasawa, Takanori Yamada, Manami Hiraiwa, Katsuyuki Kaneda and Eiichi Hinoi日期:01/05/2019,浏览量:134,Q&A: 0
Mesenchymal stem cells have the ability to differentiate into multiple lineages, including adipocytes, osteoblasts and chondrocytes. Mesenchymal stem cells can be induced to differentiate into chondrocytes in extracellular matrices, such as alginate or collagen gel. Mesenchymal stem cells in a cell pellet or micromass culture can be also induced ...
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Quantification of Mouse Hematopoietic Progenitors’ Formation Using Time-lapse Microscopy and Image Analysis 使用延时显微技术和图像分析定量小鼠造血祖细胞的形成 作者:Isabelle Bergiers, Christian Tischer, Özge Vargel Bölükbaşı and Christophe Lancrin日期:01/05/2019,浏览量:321,Q&A: 0
In vitro differentiation of mouse embryonic stem cells (mESCs) towards blood cells constitutes a well-established system to study the endothelial-to-hematopoietic transition (EHT) at the onset of blood development. Assessing the emergence of small non-adherent round blood cells in the culture without disturbing it is essential to evaluate ...
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分子生物学

Gene Mapping by RNA-sequencing: A Direct Way to Characterize Genes and Gene Expression through Targeted Queries of Large Public Databases 通过RNA测序进行基因定位:一种通过大型公共数据库目标查询来鉴定基因和基因表达的直接方法 作者:Peter Rotwein日期:01/05/2019,浏览量:215,Q&A: 0
Recent advances in genomics present new opportunities for enhancing knowledge about gene regulation and function across a wide spectrum of organisms and species. Understanding and evaluating this information at the individual gene level is challenging, and not only requires extracting, collating and interpreting data from public genetic ...
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In vitro Generation of CRISPR-Cas9 Complexes with Covalently Bound Repair Templates for Genome Editing in Mammalian Cells 利用共价修复模板体外制备用于哺乳动物细胞中基因组编辑的CRISPR-Cas9复合物 作者:Nataša Savić, Femke CAS Ringnalda, Christian Berk, Katja Bargsten, Jonathan Hall, Martin Jinek and Gerald Schwank日期:01/05/2019,浏览量:252,Q&A: 0
The CRISPR-Cas9 system is a powerful genome-editing tool that promises application for gene editing therapies. The Cas9 nuclease is directed to the DNA by a programmable single guide (sg)RNA, and introduces a site-specific double-stranded break (DSB). In mammalian cells, DSBs are either repaired by non-homologous end joining (NHEJ), generating ...
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免疫学

Adoptive Transfer of Monocytes Sorted from Bone Marrow 骨髓源性单核细胞的过继转移 作者:Damya Laoui, Eva Van Overmeire, Chloé Abels, Jiri Keirsse and Jo A Van Ginderachter日期:01/05/2019,浏览量:250,Q&A: 0
Inflammatory Ly6Chi monocytes can give rise to distinct mononuclear myeloid cells in the tumor microenvironment, such as monocytic myeloid-derived suppressor cells (Mo-MDSC), immature macrophages, M2-like tumor-associated macrophages (TAMs), M1-like TAMs or monocyte-derived dendritic cells (Mo-DCs). This protocol describes a method to ...
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神经科学

Estimation of the Readily Releasable Synaptic Vesicle Pool at the Drosophila Larval Neuromuscular Junction 果蝇幼虫神经肌肉接头中可释放突触囊泡池的估测 作者:Pragya Goel, Xiling Li and Dion Dickman日期:01/05/2019,浏览量:204,Q&A: 0
Presynaptic boutons at nerve terminals are densely packed with synaptic vesicles, specialized organelles for rapid and regulated neurotransmitter secretion. Upon depolarization of the nerve terminal, synaptic vesicles fuse at specializations called active zones that are localized at discrete compartments in the plasma membrane to initiate synaptic ...
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Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe 用阳离子JC-1染料作为敏感荧光探针进行线粒体膜电势分析 作者:Farzane Sivandzade, Aditya Bhalerao and Luca Cucullo日期:01/05/2019,浏览量:168,Q&A: 0
In recent years, fluorescent dyes have been frequently used for monitoring mitochondrial membrane potential to evaluate mitochondrial viability and function. However, the reproducibility of the results across laboratories strongly depends upon following well validated and reliable protocols along with the appropriate controls. Herein, we provide a ...
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生物物理学

Detection of Ligand-binding to Membrane Proteins by Capacitance Measurements 通过电容测量来测定膜蛋白的配体结合 作者:Verena Burtscher, Matej Hotka and Walter Sandtner日期:01/05/2019,浏览量:179,Q&A: 0
In multi-cellular organisms, cells communicate with each other utilizing chemical messengers. For many of these messenger molecules, the membrane is an insurmountable barrier. Yet, they act by binding to surface proteins often triggering a cascade of reactions inside the cell. Accordingly, studying ligand-receptor interactions at the cellular ...
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微生物学

Detection of D-glutamate Production from the Dual Function Enzyme, 4-amino-4-deoxychorismate Lyase/D-amino Acid Transaminase, in Mycobacterium smegmatis 从垢分支杆菌中通过双功能酶,4-氨基-4-脱氧胆固醇裂解酶/D-氨基酸转氨酶、转氨酶产生的D-谷氨酸的检测 作者:Helen K. Opel-Reading, Roman Mortuza and Kurt L. Krause日期:01/05/2019,浏览量:137,Q&A: 0
D-amino acid transaminase (D-AAT) is able to synthesize both D-glutamate and D-alanine, according to the following reaction: D-alanine + α-ketoglutarate ⇌ D-glutamate + pyruvate. These two D-amino acids are essential components of the peptidoglycan layer of bacteria. In our recently published work, MSMEG_5795 from Mycobacterium smegmatis ...
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植物科学

Isolation of Thylakoid Membranes from the Cyanobacterium Synechocystis sp. PCC 6803 and Analysis of Their Photosynthetic Pigment-protein Complexes by Clear Native-PAGE 从蓝藻集胞藻sp. PCC 6803中分离类囊体膜并利用CN-PAGE非变性聚丙烯酰胺凝胶电泳分析光合色素蛋白复合物 作者:Josef Komenda, Vendula Krynická and Tomas Zakar日期:01/05/2019,浏览量:153,Q&A: 0
Cyanobacteria represent a frequently used model organism for the study of oxygenic photosynthesis. They belong to prokaryotic microorganisms but their photosynthetic apparatus is quite similar to that found in algal and plant chloroplasts. The key players in light reactions of photosynthesis are Photosystem I and Photosystem II complexes (PSI and ...
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