• 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011

现期刊物(2019)

卷册: 9, 期号: 5

发育生物学

An Improved Staining Method for Low Signal LacZ Reporter Detection in Mouse Embryos 一种改进的可用于检测小鼠胚胎弱LacZ报告信号的染色法 作者:Xiaopeng Shen, Feng Xu, Shen Wu, Meng Li, Jingyi Zhang, Rui Liang and Yu Liu日期:03/05/2019,浏览量:168,Q&A: 0
In many fields of biology, especially in the field of developmental biology, LacZ reporter staining is an approach used to monitor gene expression patterns. In the LacZ reporter system, the LacZ gene is inserted in the endogenous location of the target gene via gene knock-in or by constructing a transgenic cassette in ...
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分子生物学

QsRNA-seq: A protocol for Generating Libraries for High-throughput Sequencing of Small RNAs QsRNA-seq: 小RNA高通量测序文库构建方案 作者:Alla Fishman and Ayelet T. Lamm日期:03/05/2019,浏览量:313,Q&A: 0
Small RNAs (sRNAs) are 20-30 nt long non-coding RNA molecules that regulate essentially all cellular processes. Besides being an intensively studied topic in academic research, sRNAs also hold a promise as clinical biomarkers. While the need for expressional profiling of sRNAs is growing, preparation of sRNA libraries for high-throughput ...
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干细胞

Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons 人诱导多能干细胞 (iPSCs) 分化为前脑神经祖细胞和成熟神经元的有效模型 作者:Scott Bell, Nuwan C. Hettige, Heika Silveira, Huashan Peng, Hanrong Wu, Malvin Jefri, Lilit Antonyan, Ying Zhang, Xin Zhang and Carl Ernst日期:03/05/2019,浏览量:215,Q&A: 0
Induced Pluripotent Stem Cells (iPSCs) are pluripotent stem cells that can be generated from somatic cells, and provide a way to model the development of neural tissues in vitro. One particularly interesting application of iPSCs is the development of neurons analogous to those found in the human forebrain. Forebrain neurons play a central ...
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免疫学

Characterization of Mouse Adult Testicular Macrophage Populations by Immunofluorescence Imaging and Flow Cytometry 免疫荧光成像和流式细胞术鉴定小鼠成人睾丸巨噬细胞群体 作者:Noushin Mossadegh-Keller and Michael H. Sieweke日期:03/05/2019,浏览量:215,Q&A: 0
Testicular macrophages (tMΦ) are the most abundant immune cells residing in the testis, an immune-privileged organ. TMΦ are known to exhibit different functions, such as protecting spermatozoa from auto-immune attack by producing immunosuppressive cytokines and trophic roles in supporting spermatogenesis and male sex hormone production. They also ...
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Early Life Ovalbumin Sensitization and Aerosol Challenge for the Induction of Allergic Airway Inflammation in a BALB/c Murine Model BALB/c小鼠模型中早期卵白蛋白致敏和气溶胶攻击诱导的过敏性气道炎症 作者:Kyle T Mincham, Naomi M Scott, Jean-Francois Lauzon-Joset, Jonatan Leffler, Philip A Stumbles, Patrick G Holt and Deborah H Strickland日期:03/05/2019,浏览量:185,Q&A: 0
The early life period represents a time of immunological plasticity whereby the functionally immature immune system is highly susceptible to environmental stimulation. Perennial aeroallergen and respiratory viral infection induced sporadic episodes of lung inflammation during this temporal window represent major risk factors for initiation of ...
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Quantification of Serum Ovalbumin-specific Immunoglobulin E Titre via in vivo Passive Cutaneous Anaphylaxis Assay 通过体内被动皮肤过敏试验定量血清卵清蛋白特异性免疫球蛋白E滴度 作者:Kyle T Mincham, Jonatan Leffler, Naomi M Scott, Jean-Francois Lauzon-Joset, Philip A Stumbles, Patrick G Holt and Deborah Strickland日期:03/05/2019,浏览量:163,Q&A: 0
Murine models of allergic airway disease are frequently used as a tool to elucidate the cellular and molecular mechanisms of tissue-specific asthmatic disease pathogenesis. Paramount to the success of these models is the induction of experimental antigen sensitization, as indicated by the presence of antigen-specific serum immunoglobulin E. The ...
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神经科学

Acute Cerebellar Slice Preparation Using a Tissue Chopper 使用组织切碎机快速制备小脑切片 作者:Yubin Wang and Michel Baudry日期:03/05/2019,浏览量:166,Q&A: 0
Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...
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生物化学

A Radioisotope-free Oligosaccharyltransferase Assay Method 一种非放射性同位素寡糖基转移酶测定方法 作者:Takahiro Yamasaki and Daisuke Kohda日期:03/05/2019,浏览量:172,Q&A: 0
Glycosylation of asparagine residues is widespread in Eukarya, and occurs in virtually all Archaea and some eubacterial species. A membrane-bound enzyme, oligosaccharyltransferase, catalyzes the transfer of an oligosaccharide chain from a sugar donor (lipid-linked oligosaccharide, LLO) to an asparagine residue in the consensus sequence, ...
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细胞生物学

Single-cell Motility Analysis of Tethered Human Spermatozoa 人类精子的单细胞运动分析 作者:William M. Skinner, Nadja Mannowetz, Polina V. Lishko and Nadia R. Roan日期:03/05/2019,浏览量:247,Q&A: 0
Vigorous sperm flagellar motility is essential for fertilization, and so the quantitative measurement of motility is a useful tool to assess the intrinsic fertility potential of sperm cells and explore how various factors can alter sperm’s ability to reach the egg and penetrate its protective layers. Human sperm beat their flagella many times each ...
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A New Efficient Method for Measuring Oxygen Consumption Rate Directly ex vivo in Human Epidermal Biopsies 一种直接离体测量人体表皮活检耗氧率的新方法 作者:Daniel Schniertshauer, Daniel Gebhard and Jörg Bergemann日期:03/05/2019,浏览量:188,Q&A: 0
Skin cells are constantly exposed to environmental influences such as air pollution, chemicals, pathogens and UV radiation. UV radiation can damage different biological structures, but most importantly cellular DNA. Mitochondria contain their own genome and accumulate UV-induced DNA mutations to a large extent. This can result, e.g., in ...
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植物科学

Detection of Disulfides in Protein Extracts of Arabidopsis thaliana Using Monobromobimane (mBB) 单溴二甲双胍 (mBB) 检测拟南芥蛋白质提取物中的二硫化物 作者:Shin‐nosuke Hashida and Maki Kawai-Yamada日期:03/05/2019,浏览量:176,Q&A: 0
Thiol-disulfide exchange is a key posttranslational modification, determining the folding process of intra- and inter-protein structures. Thiols can be detected by colorimetric reagents, which are stoichiometrically reduced by free thiols, and by fluorescent adducts, showing fluorescence only after thioester formation. We adapted a simple ...
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