• 卷册 9, 2019
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  • 卷册 7, 2017
  • 卷册 6, 2016
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  • 卷册 1, 2011

现期刊物(2019)

卷册: 9, 期号: 16

生物化学

A Radioactive in vitro ERK3 Kinase Assay 体外ERK3激酶的放射性分析 作者:Lobna Elkhadragy and Weiwen Long日期:08/20/2019,浏览量:74,Q&A: 0
Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases that have an important role in signal transduction. Extracellular signal-regulated kinase 3 (ERK3), also known as MAPK6, is an atypical MAPK. Here, we describe in detail an in vitro assay for the kinase activity of ERK3 using myelin basic protein (MBP) or steroid ...
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Cell-based Assay for Recruitment of DDR1 to Collagen-coated Beads 以细胞为基础的DDR1向胶原包被微珠的招募分析 作者:Victoria Juskaite and Birgit Leitinger日期:08/20/2019,浏览量:43,Q&A: 0
The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for in vitro studies. Thus, in vitro studies often use acid-soluble collagens in the ...
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癌症生物学

Three-dimensional Reconstruction and Quantification of Proteins and mRNAs at the Single-cell Level in Cultured Cells 培养细胞中蛋白和mRNA在单细胞水平上的三维重构和量化分析 作者:Xueer Jiang, Ingrid Brust-Mascher and Li-En Jao日期:08/20/2019,浏览量:249,Q&A: 0
Gene expression is often regulated by the abundance, localization, and translation of mRNAs in both space and time. Being able to visualize mRNAs and protein products in single cells is critical to understand this regulatory process. The development of single-molecule RNA fluorescence in situ hybridization (smFISH) allows the detection of ...
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细胞生物学

Fibroblast Gap-closure Assay-Microscopy-based in vitro Assay Measuring the Migration of Murine Fibroblasts 基于成纤维细胞间隙封闭显微成像的鼠成纤维细胞的体外迁移分析 作者:Agnieszka P. Looney and Mallar Bhattacharya日期:08/20/2019,浏览量:67,Q&A: 0
Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts.

Currently, the two most widely used chemotaxis and cell migration assays are the scratch assay and the ...
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A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning 利用改进的Sca/eS和机器学习用于螺旋器中毛细胞的成像和分析 作者:Shinji Urata, Tadatsune Iida, Yuri Suzuki, Shiou-Yuh Lin, Yu Mizushima, Chisato Fujimoto, Yu Matsumoto and Tatsuya Yamasoba日期:08/20/2019,浏览量:48,Q&A: 0
Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and refractive index matching, which can all be completed within 72 h. Furthermore, we established automated ...
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发育生物学

Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay 果蝇翅膀成虫盘体内培养和弗林蛋白酶抑制剂检测 作者:Alex Sohr, Lijuan Du and Sougata Roy日期:08/20/2019,浏览量:34,Q&A: 0
Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways. Classical genetic approaches and in vitro biochemical or cell biological assays identified that precursor forms of most growth factor family proteins are ...
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免疫学

In vitro Differentiation of Thymic Treg Cell Progenitors to Mature Thymic Treg Cells 体外胸腺调节性T细胞祖细胞向成熟胸腺调节性T细胞的分化 作者:David L. Owen and Michael A. Farrar日期:08/20/2019,浏览量:45,Q&A: 0
Thymic Treg cell differentiation occurs via a two-step process. Step one generates Treg cell progenitors (TregP) and is driven by strong TCR interactions with antigens presented in the thymus. Step two is initiated by activation of STAT5 via IL-2, or IL-15, leading to mature Treg cells capable of ...
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微生物学

Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation 枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定 作者:Ester Serrano and Begoña Carrasco日期:08/20/2019,浏览量:29,Q&A: 0
For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that allows cells to take up DNA from the environment. During natural chromosomal transformation, the ...
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Assessment of Metacaspase Activity in Phytoplankton 浮游植物中半胱天冬酶活性检测 作者:Dina Spungin and Ilana Berman-Frank日期:08/20/2019,浏览量:27,Q&A: 0
Programmed cell death (PCD) is an irreversible, genetically-controlled form of cell suicide in which an endogenous biochemical pathway leads to morphological changes and ultimately, cellular demise. PCD is accompanied by de-novo protein synthesis of a family of proteases-"caspases" that are often used as a diagnostic marker of PCD. ...
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Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy 细胞壁孔隙的荧光猝灭显微镜无创定量分析 作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...
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分子生物学

Genotyping of the OATP1B1 c. 521 T>C Polymorphism from the Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Specimens: An Optimized Protocol 对FFPE组织样品中OATP1B1 c. 521 T>C多态性进行基因分型的优化实验方法 作者:Alexandra R Crowe, Jonathan Miller and Wei Yue日期:08/20/2019,浏览量:40,Q&A: 0
Organic anion transporting polypeptide (OATP) 1B1 is a liver-specific transport protein that plays an important role in hepatic drug disposition. It transports many drugs from the blood into the liver, including lipid-lowering statins. The c. 521 T>C polymorphism of OATP1B1 has reduced transport activity and is associated with statin-induced ...
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Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes 一种改良的Smart-seq2样品制备方案在小鼠卵母细胞中应用稀有细胞全长单细胞mRNA测序 作者:Rebecca S. Treger, Scott D. Pope, Xiaojun Xing and Akiko Iwasaki日期:08/20/2019,浏览量:47,Q&A: 0
Endogenous retroviruses (ERV) are transposable retroelements that form ~10% of the murine genome and whose family members are differentially expressed throughout embryogenesis. However, precise regulation of ERV in germ cells remains unclear. To investigate ERV expression in oocytes, we adapted a single-cell mRNA-sequencing library preparation ...
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神经科学

Whisker Nuisance Test: A Valuable Tool to Assess Tactile Hypersensitivity in Mice 胡须损伤测试:一种检测小鼠触觉过敏症的有效方法 作者:Luigi Balasco, Gabriele Chelini, Yuri Bozzi and Giovanni Provenzano日期:08/20/2019,浏览量:82,Q&A: 0
Abnormal response to tactile stimulation, described as both hyper- and hypo-reactivity, is a common sensory impairment in multiple neuropsychiatric disorders. The neural bases of tactile sensitivity remain so far unknown. In the last years, animal studies have proven to be useful for shedding light on the cellular and molecular mechanism ...
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Construction of Viral Vectors for Cell Type-specific CRISPR Gene Editing in the Adult Mouse Brain 用于成年小鼠脑组织的细胞特异性CRISPR基因编辑的病毒载体的构建 作者:Hiroshi Yamaguchi and Luis de Lecea日期:08/20/2019,浏览量:74,Q&A: 0
Recently developed gene editing technologies based on engineered CRISPR/Cas9 systems enables researchers to disrupt genes in a cell type-specific manner in the adult mouse brain. Using these technologies, we recently showed that the dopamine beta-hydroxylase gene in Locus Coeruleus (LC) norepinephrine neurons plays a vital role in the maintenance ...
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Cylinder Test to Assess Sensory-motor Function in a Mouse Model of Parkinson’s Disease 基于圆筒试验的小鼠帕金森综合症模型中感觉运动系统功能测试 作者:Luiz Alexandre Viana Magno, Mélcar Collodetti, Helia Tenza-Ferrer and Marco Aurélio Romano-Silva日期:08/20/2019,浏览量:50,Q&A: 0
Parkinson’s disease is a progressive neurodegenerative movement disorder that happens due to the loss of dopaminergic neurons in the substantia nigra. The deficiency of dopamine in the basal nuclei drives cardinal motor symptoms such as bradykinesia and hypokinesia. The current protocol describes the cylinder test, which is a relatively simple ...
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