• 卷册 11, 2021
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  • 卷册 7, 2017
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  • 卷册 5, 2015
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Image Credit: BioProtoc.4013

现期刊物(2021)

卷册: 11, 期号: 9

生物化学

Proteomics Profiling of S-sulfurated Proteins in Acinetobacter baumannii 鲍曼不动杆菌硫酸化蛋白的蛋白质组学分析 作者:Brenna J. C. Walsh and David P. Giedroc日期:05/05/2021,浏览量:240,Q&A: 0

Hydrogen sulfide (H2S) is emerging as an important modulator in bacterial cytoprotection against the host immune response in infected animals, which may well be attributed to downstream highly oxidized sulfur species, termed reactive sulfur species (RSS), derived from H2S. One mechanism by which H2S/RSS may signal in the cell is through proteome

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Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum 恶性疟原虫无性期原位蛋白质棕榈酰化的点击化学成像 作者:Mansoor A. Siddiqui日期:05/05/2021,浏览量:387,Q&A: 0

Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases (PATs or DHHC-PATs). Palmitoylation has recently emerged as a crucial post-translational

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Chromatographic Assays for the Enzymatic Degradation of Chitin 几丁质酶解的色谱分析 作者:Sophanit Mekasha, Tina R. Tuveng, Gustav Vaaje-Kolstad and Vincent G. H. Eijsink日期:05/05/2021,浏览量:299,Q&A: 0

Chitin is an insoluble linear polymer of β(1→4)-linked N-acetylglucosamine. Enzymatic cleavage of chitin chains can be achieved using hydrolytic enzymes, called chitinases, and/or oxidative enzymes, called lytic polysaccharide monooxygenases (LPMOs). These two groups of enzymes have different modes of action and yield different product

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Characterising Plant Deubiquitinases with in vitro Activity-based Labelling and Ubiquitin Chain Disassembly Assays 植物去泛素酶的体外活性标记和泛素链分解分析 作者:Michael J. Skelly and Steven H. Spoel日期:05/05/2021,浏览量:237,Q&A: 0

Post-translational modification of proteins by ubiquitin is an essential cellular signaling mechanism in all eukaryotes. Ubiquitin is removed from target proteins by a wide range of deubiquitinase (DUB) enzymes with different activities and substrate specificities. Understanding how DUBs function in vitro is a vital first step to uncovering their

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生物医学工程

Inkjet 3D Printing of Polymers Resistant to Fungal Attachment 抗真菌附着聚合物的喷墨3D打印 作者:Yinfeng He, Cindy Vallieres, Morgan R. Alexander, Ricky D. Wildman and Simon V. Avery日期:05/05/2021,浏览量:305,Q&A: 0

Inkjet 3D printing is an additive manufacturing method that allows the user to produce a small batch of customized devices for comparative study versus commercial products. Here, we describe the use of a commercial 2D ink development system (Dimatix material printing) to manufacture small batches of 3D medical or other devices using a recently

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生物物理学

Spin Labeling of RNA Using “Click” Chemistry for Coarse-grained Structure Determination via Pulsed Electron-electron Double Resonance Spectroscopy 利用脉冲电子-电子双共振光谱测定粗粒结构的“点击”化学的RNA自旋标记 作者:Maria F. Vicino, Tobias Hett and Olav Schiemann日期:05/05/2021,浏览量:201,Q&A: 0

Understanding the function of oligonucleotides on a molecular level requires methods for studying their structure, conformational changes, and internal dynamics. Various biophysical methods exist to achieve this, including the whole toolbox of Electron Paramagnetic Resonance (EPR or ESR) spectroscopy. An EPR method widely used in this regard is

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癌症生物学

ATAC-Seq-based Identification of Extrachromosomal Circular DNA in Mammalian Cells and Its Validation Using Inverse PCR and FISH 基于ATAC-Seq的哺乳动物细胞染色体外环状DNA鉴定及其反向PCR和FISH验证 作者:Zhangli Su, Shekhar Saha, Teressa Paulsen, Pankaj Kumar and Anindya Dutta日期:05/05/2021,浏览量:330,Q&A: 0

Recent studies from multiple labs including ours have demonstrated the importance of extrachromosomal circular DNA (eccDNA) from yeast to humans (Shibata et al., 2012; Dillon et al., 2015; Møller et al., 2016; Kumar et al., 2017; Turner et al., 2017; Kim et al., 2020). More recently, it has been found that cancer cells obtain a selective

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免疫学

Analysis of Monocyte Cell Fate by Adoptive Transfer in a Murine Model of TLR7-induced Systemic Inflammation TLR7诱导的系统性炎症小鼠模型中通过过继转移单核细胞命运分析 作者:Jaba Gamrekelashvili, Hermann Haller and Florian P. Limbourg日期:05/05/2021,浏览量:282,Q&A: 0

Myeloid plasticity is a hallmark of the innate immune response to Toll-like receptor (TLR) activation. Here, we provide a protocol for monocyte cell fate tracking by adoptive transfer in the context of systemic inflammation induced by TLR7 activation, the principal innate immune receptor sensing viral RNA in mice. Defined monocyte subsets are

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微生物学

High-throughput Method for Detecting Siderophore Production by Rhizosphere Bacteria 根际细菌产生铁载体的高通量检测方法 作者:ShaoHua Gu, Wen Wan, ZhengYing Shao and Wei Zhong日期:05/05/2021,浏览量:217,Q&A: 0

Siderophores, a key substance that microorganisms produce to obtain iron under iron-limited conditions, play an important role in regulating interactions between beneficial bacteria and pathogenic bacteria. A large number of bacteria were isolated from the rhizosphere, and we used the method presented here to assay the siderophore production by

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COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR COVID-19样本汇集:从RNA提取到实时定量RT-PCR 作者:Kenny Voon, Nur Alia Johari, Khai Lone Lim, Siew Tung Wong, Loke Tim Khaw, Shew Fung Wong, Elaine W. L. Chan, Kok Keong Chan, Boon Keat Tan, Nurul Hanis Ramzi, Patricia K. C. Lim and Lokman H. Sulaiman日期:05/05/2021,浏览量:316,Q&A: 0

The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume

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Isolation of GFP-expressing Malarial Hypnozoites by Flow Cytometry Cell Sorting 流式细胞术分离表达GFP的疟原虫催眠素 作者:Annemarie Voorberg-van der Wel, Sam O. Hoffman and Clemens H. M. Kocken日期:05/05/2021,浏览量:188,Q&A: 0

Hypnozoites are dormant liver-stage parasites unique to relapsing malarial species, including the important human pathogen Plasmodium vivax, and pose a barrier to the elimination of malaria. Little is known regarding the biology of these stages, largely due to their inaccessible location. Hypnozoites can be cultured in vitro but these cultures

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Assessing Swarming of Aerobic Bacteria from Human Fecal Matter 人类粪便中需氧细菌群集的评估 作者:Arjun S. Byju, Deeti Patel, Weijie Chen and Sridhar Mani日期:05/05/2021,浏览量:200,Q&A: 0

Swarming – swift movement across a surface via flagella propulsion – is a unique property of many bacteria. The role of swarming, particularly among bacterial populations of the human gut microbiome, is not yet fully understood; although, it is becoming an area of increased scientific and clinical inquiry. To further characterize

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分子生物学

Identification of R-loop-forming Sequences in Drosophila melanogaster Embryos and Tissue Culture Cells Using DRIP-seq DRIP-seq法鉴定黑腹果蝇胚胎和组织培养细胞R-loop形成序列 作者:Célia Alecki and Nicole J. Francis日期:05/05/2021,浏览量:252,Q&A: 0

R-loops are non-canonical nucleic structures composed of an RNA–DNA hybrid and a displaced ssDNA. Originally identified as a source of genomic instability, R-loops have been shown over the last decade to be involved in the targeting of proteins and to be associated with different histone modifications, suggesting a regulatory function. In

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神经科学

Activity-based Anorexia for Modeling Vulnerability and Resilience in Mice 基于活动的厌食症小鼠脆弱性和恢复力模型的建立 作者:Jeff A. Beeler and Nesha S. Burghardt日期:05/05/2021,浏览量:201,Q&A: 0

Activity-based anorexia (ABA) is a widely used rodent model of anorexia nervosa. It involves combining limited access to food with unlimited access to a running wheel, leading to a paradoxical decrease in food intake, hyperactivity, and life-threatening weight loss. Although initially characterized in rats, ABA has been tested in mice with results

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植物科学

Age, Wound Size and Position of Injury – Dependent Vascular Regeneration Assay in Growing Leaves 年龄、伤口大小和损伤部位-——生长叶片中依赖于损伤的维管再生试验 作者:Dhanya Radhakrishnan, Anju Pallipurath Shanmukhan, Abdul Kareem, Mabel Maria Mathew, Vijina Varaparambathu, Mohammed Aiyaz, Raji Krishna Radha, Krishnaprashanth Ramesh Mekala, Anil Shaji and Kalika Prasad日期:05/05/2021,浏览量:266,Q&A: 0

Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf vasculature remain unexplored. Recently, we established a new vascular regeneration assay in

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Histological Methods to Detect Early-stage Plant Defense Responses during Artificial Inoculation of Lolium perenne with Epichloë festucae 羊茅人工接种多年生黑麦草过程中植物早期防御反应的组织学检测 作者:Mostafa Rahnama, Damien J. Fleetwood and Richard D. Johnson日期:05/05/2021,浏览量:176,Q&A: 0

Epichloë species form agriculturally important symbioses with many cool season grasses. To study these symbioses, such as the interaction of Epichloë festucae with perennial ryegrass (Lolium perenne), host plants can be infected by artificial inoculation of etiolated seedlings. This inoculation is performed by placing mycelium into an

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