• 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3725

往期刊物(2020)

卷册: 10 期号: 16

癌症生物学

Measuring Cell Growth and Junction Development in Epithelial Cells Using Electric Cell-Substrate Impedance Sensing (ECIS) 使用电子细胞基质阻抗传感(ECIS)测量上皮细胞生长和连接发育 作者:Shaista Anwer and Katalin Szaszi日期:08/20/2020,浏览量:925,Q&A: 0
Electric Cell-substrate Impedance Sensing (ECIS) is an automated method that can be used to quantify processes such as cell attachment, growth, migration and barrier functions (i.e., the properties of tight junctions). The method provides simultaneous information on cell number and tight junction function by detecting electric parameters ...
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细胞生物学

In-vitro GLP-1 Release Assay Using STC-1 Cells STC-1细胞体外释放GLP-1的实验研究 作者:Liu Qi, Tan Shuai, Jia Da, Burstein Ezra and Sifuentes-Dominguez Luis日期:08/20/2020,浏览量:585,Q&A: 0
Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.
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Stopped-flow Light Scattering Analysis of Red Blood Cell Glycerol Permeability 红细胞甘油通透性的Stopped-flow 光散射分析 作者:Patrizia Gena, Piero Portincasa, Sabino Matera, Yonathan Sonntag, Michael Rutzler and Giuseppe Calamita日期:08/20/2020,浏览量:594,Q&A: 0
Stopped-Flow Light Scattering (SFLS) is a method devised to analyze the kinetics of fast chemical reactions that result in a significant change of the average molecular weight and/or in the shape of the reaction substrates. Several modifications of the original stopped-flow system have been made leading to a significant extension of its technical ...
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免疫学

Neutrophil Extracellular Trap Killing Assay of Candida albicans 白念珠菌中性粒细胞胞外诱杀试验 作者:Sheng-Yang Wu and Betty A. Wu-Hsieh日期:08/20/2020,浏览量:695,Q&A: 0
Fungal pathogen Candida albicans is one of the top leading causes of overall healthcare-associated bloodstream infections worldwide. Neutrophil is the major effector cell to clear C. albicans infection. Our study showed that mouse neutrophils utilize two independent mechanisms to kill C. albicans: one is CR3 downstream ...
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Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-κB Reporter Mice LPS诱导NF-κB报告小鼠局部炎症及药物沉积的实时体内显像研究 作者:Artur Schmidtchen and Manoj Puthia日期:08/20/2020,浏览量:804,Q&A: 0
Wound, biomaterial, and surgical infections are all characterized by a localized and excessive inflammation, motivating the development of in vivo methods focused on the analysis of local immune events. However, current inflammation models, such as the commonly used in vivo models of endotoxin-induced inflammation are based on ...
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微生物学

In vitro Cultivation and Visualization of Malaria Liver Stages in Primary Simian Hepatocytes 猴原代肝细胞体外培养及疟疾肝期观察 作者:Devendra Kumar Gupta and Thierry Diagana日期:08/20/2020,浏览量:576,Q&A: 0
Human liver is the primary and obligatory site for malaria infection where sporozoites invade host hepatocytes. Malaria hepatic stages are asymptomatic and represent an attractive target for development of anti-malarial interventions and vaccines. However, owing to lack of robust and reproducible in vitro culture system, it is difficult ...
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Determination of the Cellular Ion Concentration in Saccharomyces cerevisiae Using ICP-AES ICP-AES法测定酿酒酵母细胞离子浓度 作者:Louise Thines, Anne Iserentant and Pierre Morsomme日期:08/20/2020,浏览量:589,Q&A: 0
The yeast Saccharomyces cerevisiae has been perceived over decades as a highly valuable model organism for the investigation of ion homeostasis. Indeed, many of the genes and biological systems that function in yeast ion homeostasis are conserved throughout unicellular eukaryotes to humans. In this context, measurement of the yeast ...
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Candida albicans Agar Invasion Assays 白念珠菌琼脂侵入实验 作者:Shamoon Naseem, Lois M. Douglas and James B. Konopka日期:08/20/2020,浏览量:587,Q&A: 0
The ability of the human fungal pathogen Candida albicans to disseminate into tissues is promoted by a switch from budding to invasive hyphal growth. This morphological transition is stimulated by multiple environmental factors that can vary at different sites of infection. To identify genes that promote invasive growth, C. albicans ...
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分子生物学

Mapping mRNA-18S rRNA Contacts Within Translation Initation Complex by Means of Reverse Transcriptase Termination Sites and RNAseq 用逆转录酶终止位点和RNAseq定位翻译起始复合体内的mRNA-18S rRNA接触点 作者:Irene Díaz-López, René Toribio and Iván Ventoso日期:08/20/2020,浏览量:568,Q&A: 0
The nucleotides involved in RNA-RNA interaction can be tagged by chemical- or UV-induced crosslinking, and further identified by classical or modern high throughput techniques. The contacts of mRNA with 18S rRNA that occur along the mRNA channel of 40S subunit have been mapped by site-specific UV crosslinking followed by reverse transcriptase ...
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Microtubule Seeded-assembly in the Presence of Poorly Nucleating Nucleotide Analogues 存在不良成核核苷酸类似物的微管种晶组装 作者:Siou Ku, Claire Heichette, Laurence Duchesne and Denis Chrétien日期:08/20/2020,浏览量:464,Q&A: 0
Microtubule dynamic instability is driven by the hydrolysis of the GTP bound to the β-subunit of the α-β tubulin heterodimer. Nucleotide analogues are commonly used to mimic the different steps of the tubulin GTPase cycle, but most of them are poor microtubule nucleators. Usually, microtubule assembly is seeded by guanylyl-(α, ...
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Isolation and Quantification of Extracellular DNA from Biofluids 生物流体中细胞外DNA的分离和定量 作者:Ľubica Janovičová, Barbora Konečná, Barbora Vlková and Peter Celec日期:08/20/2020,浏览量:619,Q&A: 0
Extracellular DNA is studied as a diagnostic biomarker, but also as a factor involved in the pathophysiology of several diseases due to its pro-inflammatory properties. Extracellular DNA can be extracted from plasma, urine, saliva or other biofluids using standard DNA isolation procedures and specialized commercial kits. Sample preparation for ...
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神经科学

A Reproducible Protocol to Measure the Critical Swimming Speed of Adult Zebrafish 一种可重复性测定成年斑马鱼临界游泳速度的方法 作者:Yuma Wakamatsu, Makoto Kashima and Hiromi Hirata日期:08/20/2020,浏览量:563,Q&A: 0
The quantitative measurement of water flow-induced swimming of fish species using a swimmill is a powerful method to evaluate motor ability of individual fish. Zebrafish is a commonly used vertebrate that enables the study of morphological, physiological and behavioral characteristics associated with genes. We here established a reproducible ...
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Assessing Gαq/15-signaling with IP-One: Single Plate Transfection and Assay Protocol for Cell-Based High-Throughput Assay 使用IP-One评估Gαq/ 15信号:基于细胞高通量测定的单板转染和测定方案 作者:Élie Besserer-Offroy, Rebecca L Brouillette, Jean-Michel Longpré and Philippe Sarret日期:08/20/2020,浏览量:632,Q&A: 0
Cell-based functional assays are an important part of compound screening and drug lead optimization, and they can also play a crucial role in the determination of the residues involved in ligand binding and signaling for a particular G-protein-coupled receptor. Conventional methods used for Gαq/15-coupled receptors rely on the use of ...
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Co-culture of Murine Neurons Using a Microfluidic Device for The Study of Tau Misfolding Propagation 利用微流体装置共培养小鼠神经元研究Tau的错误折叠传播 作者:Grace I Hallinan, Dianne M Lopez, Mariana Vargas-Caballero, Jonathan West and Katrin Deinhardt日期:08/20/2020,浏览量:698,Q&A: 0
The deposition of misfolded, aggregated tau protein is a hallmark of several neurodegenerative diseases, collectively termed “tauopathies”. Tau pathology spreads throughout the brain along connected pathways in a prion-like manner. The process of tau pathology propagation across circuits is a focus of intense research and has been investigated in ...
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Sequential Reaching Task for the Study of Motor Skills in Monkeys 猴子运动技能研究的连续性达标任务 作者:Machiko Ohbayashi and Nathalie Picard日期:08/20/2020,浏览量:426,Q&A: 0
The ability to perform a sequence of movements is a key component of motor skills, such as typing or playing a musical instrument. How the brain binds elementary movements together into meaningful actions has been a topic of much interest. Here, we describe two sequential reaching tasks that we use to investigate the neural substrate of skilled ...
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Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUS 核转运蛋白-β2抑制和ALS / FTD相关蛋白FUS的逆转聚集和液-液相分离 作者:Emma Robinson, James Shorter and Lin Guo日期:08/20/2020,浏览量:758,Q&A: 0
The study of RNA-binding proteins (RBP) offers insight into the mechanisms of pathologic protein aggregation in neurodegenerative diseases. We developed a protocol for purifying an RBP FUS and a nuclear import receptor (NIR) Kapβ2 and testing the ability of Kapβ2 to mitigate FUS aggregation and liquid-liquid phase separation.
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