• 卷册 11, 2021
  • 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3975

往期刊物(2021)

卷册: 11 期号: 7

生物化学

Expression and Purification of the Human Cation-chloride Cotransporter KCC1 from HEK293F Cells for Structural Studies 从HEK293F细胞中人K-Cl转运蛋白KCC1的表达和纯化用于结构研究 作者:Si Liu and Jiangtao Guo日期:04/05/2021,浏览量:304,Q&A: 0

Cation-chloride cotransporters (CCCs) mediate the coupled, electroneutral symport of cations such as Na+ and/or K+ with chloride across membrane. Among CCCs family, K-Cl cotransporters (KCC1-KCC4) extrude intracellular Cl- by the transmembrane K+ gradient. In humans, these KCCs play vital roles in the physiology of the nervous system and kidney.

...
更多 >>

生物物理学

FRET-based Microscopy Assay to Measure Activity of Membrane Amino Acid Transporters with Single-transporter Resolution 用荧光共振能量转移的显微技术测定单转运体膜氨基酸转运体的活性 作者:Didar Ciftci, Gerard H. M. Huysmans, Xiaoyu Wang, Changhao He, Daniel Terry, Zhou Zhou, Gabriel Fitzgerald, Scott C. Blanchard and Olga Boudker日期:04/05/2021,浏览量:499,Q&A: 0

Secondary active transporters reside in cell membranes transporting polar solutes like amino acids against steep concentration gradients, using electrochemical gradients of ions as energy sources. Commonly, ensemble-based measurements of radiolabeled substrate uptakes or transport currents inform on kinetic parameters of transporters. Here we

...
更多 >>
Developing Biohybrid Robotic Jellyfish (Aurelia aurita) for Free-swimming Tests in the Laboratory and in the Field 开发的生物合成机器人水母在室内和室外的自由游泳测试 作者:Nicole W. Xu, James P. Townsend, John H. Costello, Sean P. Colin, Brad J. Gemmell and John O. Dabiri日期:04/05/2021,浏览量:311,Q&A: 0

Biohybrid robotics is a growing field that incorporates both live tissues and engineered materials to build robots that address current limitations in robots, including high power consumption and low damage tolerance. One approach is to use microelectronics to enhance whole organisms, which has previously been achieved to control the locomotion of

...
更多 >>

癌症生物学

Atomic Force Microscopy to Characterize Ginger Lipid-Derived Nanoparticles (GLDNP) 原子力显微镜研究生姜脂质纳米颗粒(GLDNP) 作者:Dingpei Long, Chunhua Yang, Junsik Sung and Didier Merlin日期:04/05/2021,浏览量:310,Q&A: 0

We have demonstrated that a specific population of ginger-derived nanoparticles (GDNP-2) could effectively target the colon, reduce colitis, and alleviate colitis-associated colon cancer. Naturally occurring GDNP-2 contains complex bioactive components, including lipids, proteins, miRNAs, and ginger secondary metabolites (gingerols and shogaols).

...
更多 >>

发育生物学

Analysis of TORC1-body Formation in Budding Yeast 芽殖酵母TORC1-body形成的分析 作者:Ryan L. Wallace, Eric Lu, Arron Sullivan, James E. Hughes Hallett and Andrew P. Capaldi日期:04/05/2021,浏览量:366,Q&A: 0

The Target of Rapamycin kinase Complex I (TORC1) is the master regulator of cell growth and metabolism in eukaryotes. In the presence of pro-growth hormones and abundant nutrients, TORC1 is active and drives protein, lipid, and nucleotide synthesis by phosphorylating a wide range of proteins. In contrast, when nitrogen and/or glucose levels fall,

...
更多 >>
A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy 用常规和体积电子显微镜对秀丽隐杆线虫胚胎进行超微结构分析的高压冷冻和冷冻替代的工作流程 作者:Mohammad M. Rahman, Irene Y. Chang, Orna Cohen-Fix and Kedar Narayan日期:04/05/2021,浏览量:424,Q&A: 0

The free-living nematode Caenorhabditis elegans is a popular model system for studying developmental biology. Here we describe a detailed protocol to high-pressure freeze the C. elegans embryo (either ex vivo after dissection, or within the intact worm) followed by quick freeze substitution. Processed samples are suitable for ultrastructural

...
更多 >>

免疫学

A Potent Vaccine Delivery System 一个有效的疫苗输送系统 作者:Guangzu Zhao, Armira Azuar, Istvan Toth and Mariusz Skwarczynski日期:04/05/2021,浏览量:412,Q&A: 0

Most vaccines require co-delivery of an adjuvant in order to generate the desired immune responses. However, many currently available adjuvants are non-biodegradable, have limited efficacy, and/or poor safety profile. Thus, new adjuvants, or self-adjuvanting vaccine delivery systems, are required. Here, we proposed a self-adjuvanting delivery

...
更多 >>
Brain-localized and Intravenous Microinjections in the Larval Zebrafish to Assess Innate Immune Response 对斑马鱼幼体进行脑局部和静脉显微注射以评估先天免疫反应 作者:Alison M. Rojas and Celia E. Shiau日期:04/05/2021,浏览量:610,Q&A: 0

Creating a robust and controlled infection model is imperative for studying the innate immune response. Leveraging the particular strengths of the zebrafish model system, such as optical transparency, ex utero development, and large clutch size, allows for the development of methods that yield consistent and reproducible results. We created a

...
更多 >>
Using the Cecal Ligation and Puncture Model of Sepsis to Induce Rats to Multiple Organ Dysfunction 采用败血症盲肠结扎穿刺模型诱导大鼠多脏器功能障碍 作者:Jose Manuel Condor Capcha, Roberto S. Moreira, Camila E. Rodrigues, Marcelo A. D. Silveira, Lucia Andrade and Samirah A. Gomes日期:04/05/2021,浏览量:368,Q&A: 0

Sepsis is a dysregulated hyperinflammatory disease caused by infection. Sepsis leads to multiple organ dysfunction syndrome (MODS), which is associated with high rates of mortality. The cecal ligation and puncture (CLP) model has been widely used in animals and has become the gold-standard method of replicating features of sepsis in humans.

...
更多 >>
Ex vivo Assessment of Mitochondrial Function in Human Peripheral Blood Mononuclear Cells Using XF Analyzer 使用XF分析仪体外评估人外周血单个核细胞线粒体功能 作者:Alica Schöller-Mann, Katja Matt, Barbara Hochecker and Jörg Bergemann日期:04/05/2021,浏览量:280,Q&A: 0

Cellular health and function, as we know today, depend on a large extent on mitochondrial function. The essential function of mitochondria is the energy production, more precisely ATP production, via oxidative phosphorylation. Mitochondrial energy production parameters therefore represent important biomarkers. Studies on human cells have mainly

...
更多 >>

分子生物学

Reconstitution of Chromatin by Stepwise Salt Dialysis 逐步盐透析重组染色质 作者:Grisel Cruz-Becerra and James T. Kadonaga日期:04/05/2021,浏览量:312,Q&A: 0

Chromatin, rather than plain DNA, is the natural substrate of the molecular machines that mediate DNA-directed processes in the nucleus. Chromatin can be reconstituted in vitro by using different methodologies. The salt dialysis method yields chromatin that consists of purified histones and DNA. This biochemically pure chromatin is well-suited for

...
更多 >>

神经科学

Imaging Microtubules in vitro at High Resolution while Preserving their Structure 保留微管结构同时在体外对其进行高分辨率成像 作者:Camille Cuveillier, Yasmina Saoudi, Isabelle Arnal and Christian Delphin日期:04/05/2021,浏览量:305,Q&A: 0

Microtubules (MT) are the most rigid component of the cytoskeleton. Nevertheless, they often appear highly curved in the cellular context and the mechanisms governing their overall shape are poorly understood. Currently, in vitro microtubule analysis relies primarily on electron microscopy for its high resolution and Total Internal Reflection

...
更多 >>
Cranioplastic Surgery and Acclimation Training for Awake Mouse fMRI 清醒小鼠fMRI的颅成形手术及适应训练 作者:Tomokazu Tsurugizawa, Kota Tamada, Clement Debacker, Andrew Zalesky and Toru Takumi日期:04/05/2021,浏览量:313,Q&A: 0

MRI is a promising tool for translational research to link brain function and structure in animal models of disease to patients with neuropsychiatric disorders. However, given that mouse functional MRI (fMRI) typically relies on anesthetics to suppress head motion and physiological noise, it has been difficult to directly compare brain fMRI in

...
更多 >>

植物科学

In vitro Reconstitution Assays of Arabidopsis 20S Proteasome 拟南芥20S蛋白酶体的体外重组分析 作者:Yanjun Li, Di Sun, Xingxing Yan, Zhiye Wang and Xiuren Zhang日期:04/05/2021,浏览量:321,Q&A: 0

The majority of cellular proteins are degraded by the 26S proteasome in eukaryotes. However, intrinsically disordered proteins (IDPs), which contain large portions of unstructured regions and are inherently unstable, are degraded via the ubiquitin-independent 20S proteasome. Emerging evidence indicates that plant IDP homeostasis may also be

...
更多 >>
A Novel Method to Construct Binary CRISPR Vectors for Plant Transformation by Single Round of PCR Amplification 一种通过单轮PCR扩增构建用于植物转化的二元CRISPR载体的新方法 作者:Kang Li, Yuhui Wang and Chuanying Fang日期:04/05/2021,浏览量:552,Q&A: 0

CRISPR/Cas9 is an established and flexible tool for genome editing. However, most methods used to generate expression clones for the CRISPR/Cas9 are time-consuming. Hence, we have developed a one-step protocol to introduce sgRNA expression cassette(s) directly into binary vectors (Liu et al., 2020). In this approach, we have optimized the

...
更多 >>
Measurements of Root Colonized Bacteria Species 根定植细菌种类的测量 作者:Vílchez Juan Ignacio, Yu Yang, Dian Yi and Huiming Zhang日期:04/05/2021,浏览量:594,Q&A: 0

Root-associated bacteria are able to influence plant fitness and vigor. A key step in understanding the belowground plant-bacteria interactions is to quantify root colonization by the bacteria of interest. Probably, genetic engineering with fluorescence markers is the most powerful way to monitor bacterial strains in plant. However, this could

...
更多 >>