• 卷册 11, 2021
  • 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.4080

往期刊物(2021)

卷册: 11 期号: 13

生物化学

Visualization of Host Cell Kinase Activation by Viral Proteins Using GFP Fluorescence Complementation and Immunofluorescence Microscopy 利用GFP荧光互补和免疫荧光显微镜观察病毒蛋白对宿主细胞激酶的激活 作者:Sherry T. Shu, Wing Fai Li and Thomas E. Smithgall日期:07/05/2021,浏览量:1695,Q&A: 0

Non-receptor protein-tyrosine kinases regulate cellular responses to many external signals and are important drug discovery targets for cancer and infectious diseases. While many assays exist for the assessment of kinase activity in vitro, methods that report changes in tyrosine kinase activity in single cells have the potential to provide

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Yeast Lipid Extraction and Analysis by HPTLC 酵母脂质的HPTLC提取及分析 作者:Dan Li, Zheng-Tan Zhang, Cheng-Wen He and Zhiping Xie日期:07/05/2021,浏览量:1107,Q&A: 0

The diversity of lipid structures, properties, and combinations in biological tissues makes their extraction and analysis an experimental challenge. Accordingly, even for one of the simplest single-cellular fungi, the budding yeast (Saccharomyces cerevisiae), numerous extraction and analysis protocols have been developed to separate and quantitate

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生物医学工程

Building a Total Internal Reflection Microscope (TIRF) with Active Stabilization (Feedback SMLM) 建立一个主动稳定的全内反射显微镜(TIRF) 作者:Simao Coelho, Jongho Baek, J. Justin Gooding and Katharina Gaus日期:07/05/2021,浏览量:1823,Q&A: 0

The data quality of high-resolution imaging can be markedly improved with active stabilization, which is based on feedback loops within the microscope that maintain the sample in the same location throughout the experiment. The purpose is to provide a highly accurate focus lock, therefore eliminating drift and improving localization precision.

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生物物理学

Single-molecule Fluorescence Technique to Monitor the Co-transcriptional Formation of G-quadruplex and R-loop Structures 单分子荧光技术监测G-四链体和R-环结构的共转录形成 作者:Gunhyoung Lim and Sungchul Hohng日期:07/05/2021,浏览量:1625,Q&A: 0

G-quadruplexes (GQ) and R-loops are non-canonical nucleic acid structures related to gene regulation and genome instability that can be formed during transcription; however, their formation mechanisms remain elusive. To address this question, we developed a single-molecule fluorescence technique to monitor the formation of G-quadruplex and R-loop

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OrganoPlate Micro-fluidic Microvessel Culture and Analysis OrganoPlate微流控微血管培养与分析 作者:Abidemi Junaid and Thomas Hankemeier日期:07/05/2021,浏览量:1444,Q&A: 0

The endothelial cells from the microvasculature are key drivers and targets of inflammatory and thrombotic processes in microvascular diseases. The study of bioactive lipids in inflammatory processes has been largely based on two-dimensional endothelial cell cultures. Three-dimensional microvessels-on-a-chip provides an opportunity to monitor the

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癌症生物学

Monitoring Changes in the Oxidizing Milieu in the Endoplasmic Reticulum of Mammalian Cells Using HyPerER 利用HyPerER监测哺乳动物细胞内质网氧化环境的变化 作者:Julia Birk, Beata Lizak, Christian Appenzeller-Herzog and Alex Odermatt日期:07/05/2021,浏览量:1442,Q&A: 0

The production of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress are tightly linked. The generation of ROS can be both the cause and a consequence of ER stress pathways, and an increasing number of human diseases are characterized by tissue atrophy in response to ER stress and oxidative injury. For the assessment of modulators

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细胞生物学

A Method for Estimating the Potential Synaptic Connections Between Axons and Dendrites From 2D Neuronal Images 一种从二维神经元图像中估计轴突和树突之间潜在突触连接的方法 作者:Carolina Tecuatl, Diek W. Wheeler and Giorgio A. Ascoli日期:07/05/2021,浏览量:1570,Q&A: 0

Computational neuroscience aims to model, reproduce, and predict network dynamics for different neuronal ensembles by distilling knowledge derived from electrophysiological and morphological evidence. However, analyses and simulations often remain critically limited by the sparsity of direct experimental constraints on essential parameters, such

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Electron Tomography to Study the Three-dimensional Structure of the Reovirus Egress Pathway in Mammalian Cells 电子断层扫描研究哺乳动物细胞呼肠孤病毒出口途径的三维结构 作者:Isabel Fernández de Castro, Jose Jesús Fernández, Terence S. Dermody and Cristina Risco日期:07/05/2021,浏览量:1397,Q&A: 0

Mammalian orthoreoviruses (reoviruses) are nonenveloped, double-stranded RNA viruses that replicate and assemble in cytoplasmic membranous organelles called viral inclusions (VIs). To define the cellular compartments involved in nonlytic reovirus egress, we imaged viral egress in infected, nonpolarized human brain microvascular endothelial cells

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Ethanol-induced Sedative Behavior: An Assay to Investigate Increased Dopamine Signaling in Caenorhabditis elegans 乙醇诱导的镇静行为:一项在秀丽隐杆线虫研究增加多巴胺信号 作者:Anuradha Singh, Kavita Babu and Pratima Pandey日期:07/05/2021,浏览量:870,Q&A: 0

Dopamine (DA) signaling affects locomotion, feeding, learning, and memory in C. elegans. Various assays have been developed to study the proteins involved in these behaviors; however, these assays show behavioral output only when there is a drastic change in DA levels. We designed an assay capable of observing behavioral output even with only

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微生物学

Production, quantification, and infection of Amazonian Phlebovirus (Bunyaviridae) 亚马孙流域白蛉病毒属(布尼亚病毒科)的产生、定量和感染 作者:Carolina Torturella Rath, Aislan de Carvalho Vivarini, Renata M. Pereira and Ulisses Gazos Lopes日期:07/05/2021,浏览量:1324,Q&A: 0

Phlebotomine vectors, sand flies of the order Diptera, are known to transmit Leishmania parasites as well as RNA viruses (arboviruses) to humans. The arbovirus, Icoaraci Phlebovirus (BeAN 24262 - ICOV), used in this study was isolated from Nectomys rodents, a mammalian species that is the same natural sylvatic reservoir of Leishmania (Leishmania)

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分子生物学

Measurement of Transgene Copy Number in Plants Using Droplet Digital PCR 应用微滴数字PCR技术测定植物中的转基因拷贝数 作者:Yao-Min Cai, Quentin M. Dudley and Nicola J. Patron日期:07/05/2021,浏览量:1297,Q&A: 0

Transgenic plants are produced both to investigate gene function and to confer desirable traits into crops. Transgene copy number is known to influence expression levels, and consequently, phenotypes. Similarly, knowledge of transgene zygosity is desirable for making quantitative assessments of phenotype and tracking the inheritance of transgenes

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PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System CRISPR/Cas9系统的PCR介导的一日合成向导RNA 作者:Naim Hassan, Farhana Easmin, Keisuke Ekino and Satoshi Harashima日期:07/05/2021,浏览量:1522,Q&A: 0

Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is indispensable for the CRISPR/Cas9 system. To express gRNA, a plasmid expressing the gRNA sequence is

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神经科学

An Improved Method for Individual Tracking of Voluntary Wheel Running in Pair-housed Juvenile Mice 一种对饲养的幼鼠自主跑轮运动个体跟踪的改进方法 作者:David A. Valientes, Anthony M. Raus and Autumn S. lvy日期:07/05/2021,浏览量:926,Q&A: 0

Rodent cages equipped with access to a voluntary running wheel are commonly used to study the effects of aerobic physical activity on physiology and behavior. Notable discoveries in exercise neurobiology, including the key role of brain-derived neurotrophic factor (BDNF) in neural plasticity and cognition, have been made using rodents housed with

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Immunofluorescence of GFAP and TNF-α in the Mouse Hypothalamus GFAP和TNF-α在小鼠下丘脑的免疫荧光 作者:Prasad S. Dalvi and Denise D. Belsham日期:07/05/2021,浏览量:944,Q&A: 0

Immunofluorescence is a reliable method for identifying specific proteins in neuronal and glial cell populations of the hypothalamus. Several immunofluorescence protocols are available to detect protein markers and neuropeptides in the hypothalamus; however, published methods may vary in subtle details that can potentially impact the final outcome

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Evaluating Baseline and Sensitised Heat Nociception in Adult Drosophila 评估成年果蝇的基线和致敏热伤害感受 作者:Josephine N. Massingham, Olga Baron and G. Gregory Neely日期:07/05/2021,浏览量:752,Q&A: 0

Chronic pain is a complex disease that affects a large proportion of the population. With little to no effective treatments currently available for patients, this malady presents a large burden to society. Drosophila melanogaster has been previously used to describe conserved molecular components of nociception in larvae and adults. However, adult

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植物科学

Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS 气质联用技术分析水稻组织中微量可溶性糖含量 作者:Li-he Xu, Li-ying Xiao, Xue-qiong Xiao, Yan-nong Xiao and Gao-feng Wang日期:07/05/2021,浏览量:1480,Q&A: 0

Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies have been developed to quantitate soluble sugar content in plant tissues; however, it is difficult

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