哺乳纲

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    现刊
    A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
    利用改进的Sca/eS和机器学习用于螺旋器中毛细胞的成像和分析
    [Abstract] Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and refractive index matching, which can all be completed within 72 h. Furthermore, we established automated ...
    Mouse Cochlear Whole Mount Immunofluorescence
    小鼠耳蜗整装包埋免疫荧光法
    作者:Omar Akil and Lawrence R. Lustig日期:03/05/2013,浏览量:23718,Q&A: 2
    [Abstract] This protocol comprises the entire process of immunofluorescence staining on mouse cochlea whole mount, starting from tissue preparation to the mounting of the tissue. This technique provides “three-dimensional” views of the stained components in order to determine the localization of a protein of interest in the tissue in its natural state and ...
    IgE-mediated Chronic Allergic Inflammation (IgE-CAI)
    免疫球蛋白介导的慢性过敏性炎症小鼠模型(IgE-CAI)
    作者:Yasuyo Harada and Masato Kubo日期:10/05/2012,浏览量:9535,Q&A: 0
    [Abstract] This system is one of a chronic allergic inflammation model. A single subcutaneous injection of antigens elicits a delayed-type response manifested by ear swelling. The entire response consists of three phases: Immediate, late, and chronic. The early-phase swelling peak within 1 h after allergen challenge is followed by the late-phase swelling ...