原核生物

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    现刊
    Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation
    枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定
    作者:Ester Serrano and Begoña Carrasco日期:08/20/2019,浏览量:29,Q&A: 0
    For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that ...
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
    All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the ...
    Assessment of Metacaspase Activity in Phytoplankton
    浮游植物中半胱天冬酶活性检测
    作者:Dina Spungin and Ilana Berman-Frank日期:08/20/2019,浏览量:27,Q&A: 0
    Programmed cell death (PCD) is an irreversible, genetically-controlled form of cell suicide in which an endogenous biochemical pathway leads to morphological changes and ultimately, cellular demise. PCD is accompanied by de-novo protein ...
    Biofilm Assays on Fibrinogen-coated Silicone Catheters and 96-well Polystyrene Plates
    在纤维蛋白原包覆的硅胶导管和96孔聚苯乙烯板上进行生物膜检测
    作者:Cristina Colomer-Winter, José A. Lemos and Ana L. Flores-Mireles日期:03/20/2019,浏览量:1094,Q&A: 0
    Biofilm formation is a well-known bacterial strategy that protects cells from hostile environments. During infection, bacteria found in a biofilm community are less sensitive to antibiotics and to the immune response, often allowing them to colonize ...
    Quantification of Queuosine Modification Levels in tRNA from Human Cells Using APB Gel and Northern Blot
    利用聚乙二醇凝胶和Northern印记法定量分析来自人细胞的tRNA 的Q核苷修饰水平
    作者:Zaneta Matuszek and Tao Pan日期:03/20/2019,浏览量:894,Q&A: 0
    Queuosine (Q) is a hypermodified base in the wobble anticodon position of tRNAs coding for the amino acids Tyr, His, Asn, and Asp. tRNA Q-modification is introduced by a queuine tRNA-ribosyltransferase (TGT) that replaces the guanine base at G34 at ...
    Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation
    枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定
    作者:Ester Serrano and Begoña Carrasco日期:08/20/2019,浏览量:29,Q&A: 0
    [Abstract] For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that allows cells to take up DNA from the environment. During natural chromosomal transformation, the ...
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
    [Abstract] All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...
    Assessment of Metacaspase Activity in Phytoplankton
    浮游植物中半胱天冬酶活性检测
    作者:Dina Spungin and Ilana Berman-Frank日期:08/20/2019,浏览量:27,Q&A: 0
    [Abstract] Programmed cell death (PCD) is an irreversible, genetically-controlled form of cell suicide in which an endogenous biochemical pathway leads to morphological changes and ultimately, cellular demise. PCD is accompanied by de-novo protein synthesis of a family of proteases-"caspases" that are often used as a diagnostic marker of PCD. ...
    Optimized Protocol for the Incorporation of FDAA (HADA Labeling) for in situ Labeling of Peptidoglycan
    FDAA用于肽聚糖原位标记的优选方案
    作者:Katharina Peters, Manuel Pazos, Michael S. VanNieuwenhze and Waldemar Vollmer日期:08/05/2019,浏览量:213,Q&A: 0
    [Abstract] The essential peptidoglycan (PG) layer surrounds the cytoplasmic membrane in nearly all bacteria. It is needed to maintain the shape of the cell and protect it from lysis due to high turgor. Growth of the PG layer is a complex process that involves the activities of PG synthases and hydrolases during elongation and cell division. PG growth sites ...
    Strand-specific Single-stranded DNA Sequencing (4S-seq) of E. coli genomes
    大肠杆菌特异性单链DNA测序(4S-seq)
    作者:Takahiro Masuda, Nobuaki Kono, Masaru Tomita and Kazuharu Arakawa日期:08/05/2019,浏览量:215,Q&A: 0
    [Abstract] Most bacterial genomes have biased nucleotide composition, and the asymmetry is considered to be caused by a single-stranded DNA (ssDNA) deamination arising from the bacterial replication machinery. In order to evaluate the relationship experimentally, the position and frequency of ssDNA formed during replication must be verified clearly. Although ...
    Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
    蚀斑测定弗氏志贺在TC7人肠上皮细胞中的侵袭和细胞间传播
    作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:445,Q&A: 0
    [Abstract] Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to gain access to the cytosol. Thereafter Shigella disseminates to neighboring cells in an ...
    Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri
    庆大霉素保护试验确定福氏志贺菌侵染人TC7肠上皮细胞的胞内寄生数目
    作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:527,Q&A: 0
    [Abstract] Shigella flexneri is an intracellular bacterial pathogen that gains access to the gut epithelium using a specialized Type III Secretion System (T3SS). Various determinants mediating this invasive infection have been experimentally verified using the classical gentamicin protection assay presented here. In this assay epithelial cell lines ...
    In vitro and in vivo Assessment of Protein Acetylation Status in Mycobacteria
    分枝杆菌中蛋白乙酰化状态的体外和体内检测
    作者:Krishna K. Singh, Devendra P. Singh, Rambir Singh and Deepak K. Saini日期:07/05/2019,浏览量:471,Q&A: 0
    [Abstract] Protein acetylation is one of the standard post-translational modifications found in proteins across all organisms, along with phosphorylation which regulates diverse cellular processes. Acetylation of proteins can be enzymatically catalyzed through acetyltransferases, acetyl CoA synthetases or non-enzymatically through acyl carrier metabolic ...
    Preparation and Purification of Active Recombinant Human Pancreatic Lipase in Escherichia coli
    重组人胰脂肪酶在大肠杆菌中的表达和纯化
    作者:Nanami Kawaguchi, Haruko Ogawa and Kimie Date日期:07/05/2019,浏览量:510,Q&A: 0
    [Abstract] Human pancreatic lipase (HPL) is the main lipolytic enzyme involved in the digestion of dietary fat. An active recombinant human pancreatic lipase (recHPL) was successfully prepared for the first time in an Escherichia coli (E. coli) expression system using a short Strep-tag II (ST II). The recHPL-ST II was solubilized with 8 M urea from ...
    Imaging Gene Expression Dynamics in Pseudomonas fluorescens In5 during Interactions with the Fungus Fusarium graminearum PH-1
    荧光假单胞菌In5与小麦赤霉菌PH-1互作过程中的基因表达动态成像
    作者:Rosanna C. Hennessy, Peter Stougaard and Stefan Olsson日期:06/20/2019,浏览量:588,Q&A: 0
    [Abstract] Genomics, transcriptomics and metabolomics are powerful technologies for studying microbial interactions. The main drawback of these methods is the requirement for destructive sampling. We have established an alternative but complementary technique based on a microplate system combined with promoter fusions for visualizing gene expression in space ...