微生物学

分类

    现刊
    Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation
    枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定
    作者:Ester Serrano and Begoña Carrasco日期:08/20/2019,浏览量:29,Q&A: 0
    For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that ...
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
    All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the ...
    Assessment of Metacaspase Activity in Phytoplankton
    浮游植物中半胱天冬酶活性检测
    作者:Dina Spungin and Ilana Berman-Frank日期:08/20/2019,浏览量:27,Q&A: 0
    Programmed cell death (PCD) is an irreversible, genetically-controlled form of cell suicide in which an endogenous biochemical pathway leads to morphological changes and ultimately, cellular demise. PCD is accompanied by de-novo protein ...
    Viral Chromosome Conformation Capture (V3C) Assays for Identifying Trans-interaction Sites between Lytic Viruses and the Cellular Genome
    病毒染色体构象俘获技术用于裂解病毒和细胞基因组之间反向相互作用位点的检测
    作者:Kinjal Majumder, Maria Boftsi and David J Pintel日期:03/20/2019,浏览量:868,Q&A: 0
    The folding mechanisms of the mammalian genome package our genetic material into the nucleus, and in doing so, dictate its appropriate replication and expression. Chromosome conformation capture technology has enabled the dissection of the folding ...
    Biofilm Assays on Fibrinogen-coated Silicone Catheters and 96-well Polystyrene Plates
    在纤维蛋白原包覆的硅胶导管和96孔聚苯乙烯板上进行生物膜检测
    作者:Cristina Colomer-Winter, José A. Lemos and Ana L. Flores-Mireles日期:03/20/2019,浏览量:1094,Q&A: 0
    Biofilm formation is a well-known bacterial strategy that protects cells from hostile environments. During infection, bacteria found in a biofilm community are less sensitive to antibiotics and to the immune response, often allowing them to colonize ...
    Quantification of Queuosine Modification Levels in tRNA from Human Cells Using APB Gel and Northern Blot
    利用聚乙二醇凝胶和Northern印记法定量分析来自人细胞的tRNA 的Q核苷修饰水平
    作者:Zaneta Matuszek and Tao Pan日期:03/20/2019,浏览量:894,Q&A: 0
    Queuosine (Q) is a hypermodified base in the wobble anticodon position of tRNAs coding for the amino acids Tyr, His, Asn, and Asp. tRNA Q-modification is introduced by a queuine tRNA-ribosyltransferase (TGT) that replaces the guanine base at G34 at ...
    Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation
    枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定
    作者:Ester Serrano and Begoña Carrasco日期:08/20/2019,浏览量:29,Q&A: 0
    [Abstract] For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that allows cells to take up DNA from the environment. During natural chromosomal transformation, the ...
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
    [Abstract] All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...
    Assessment of Metacaspase Activity in Phytoplankton
    浮游植物中半胱天冬酶活性检测
    作者:Dina Spungin and Ilana Berman-Frank日期:08/20/2019,浏览量:27,Q&A: 0
    [Abstract] Programmed cell death (PCD) is an irreversible, genetically-controlled form of cell suicide in which an endogenous biochemical pathway leads to morphological changes and ultimately, cellular demise. PCD is accompanied by de-novo protein synthesis of a family of proteases-"caspases" that are often used as a diagnostic marker of PCD. ...
    Optimized Protocol for the Incorporation of FDAA (HADA Labeling) for in situ Labeling of Peptidoglycan
    FDAA用于肽聚糖原位标记的优选方案
    作者:Katharina Peters, Manuel Pazos, Michael S. VanNieuwenhze and Waldemar Vollmer日期:08/05/2019,浏览量:213,Q&A: 0
    [Abstract] The essential peptidoglycan (PG) layer surrounds the cytoplasmic membrane in nearly all bacteria. It is needed to maintain the shape of the cell and protect it from lysis due to high turgor. Growth of the PG layer is a complex process that involves the activities of PG synthases and hydrolases during elongation and cell division. PG growth sites ...
    Visualizing Hypoxia in a Murine Model of Candida albicans Infection Using in vivo Biofluorencence
    用活体生物荧光法观察小鼠白念珠菌感染模型中的缺氧情况
    作者:José Pedro Lopes and Constantin F. Urban日期:08/05/2019,浏览量:292,Q&A: 0
    [Abstract] Candida albicans is a leading human fungal pathogen that uses several metabolic adaptations to escape immune cells and causes systemic disease. Here, we describe a protocol for measuring one of these adaptations, the ability to thrive in hypoxic niches. Hypoxia was generated after successful subdermal infection with C. albicans ...
    Solid Phase PCR on 3D Microstructure ArrayChip for Pathogen Detection Application
    用于病原体检测的三维显微组织列阵中固相PCR
    作者:Krishna Kant and Tien Anh Ngo日期:08/05/2019,浏览量:208,Q&A: 0
    [Abstract] Advanced free angle photolithography (FAPL) is presented for making 3D supercritical angle fluorescence (SAF) microstructures and transfer them on to polymeric chips using injection molding technique for low-cost microfluidic devices embedded with optical sensing structures. A solid phase polymerase chain reaction (SP-PCR) is used as model ...
    Image-based Quantification of Direct Cell-to-cell Transmission of Bovine Viral Diarrhea Virus
    基于成像量化分析牛病毒性腹泻病毒的细胞间传播
    作者:Fernando Merwaiss and Diego E. Alvarez日期:08/05/2019,浏览量:189,Q&A: 0
    [Abstract] Different viruses rely on direct cell-to-cell transmission to propagate infection within the infected host. Measuring this mode of transmission in cultured cells is often complicated by the contribution of cell free viruses to spread, and the difficulty to distinguish between primary infected cells that produce the virus and neighboring cells that ...
    QUEEN-based Spatiotemporal ATP Imaging in Budding and Fission Yeast
    发芽酵母和裂殖酵母中基于QUEEN的时空ATP成像
    作者:Masak Takaine日期:08/05/2019,浏览量:246,Q&A: 0
    [Abstract] Yeasts have provided an exceptional model for studying metabolism and bioenergetics in eukaryotic cells. Among numerous metabolites, adenosine triphosphate (ATP) is a major metabolite that is essential for all living organisms. Therefore, a clearer understanding of ATP dynamics in living yeast cells is important for deciphering cellular energy ...
    Endocytosis Detection in Magnaporthe oryzae
    稻瘟病菌内吞作用的检测
    作者:Muxing Liu and Zhengguang Zhang日期:08/05/2019,浏览量:188,Q&A: 0
    [Abstract] Endocytosis is an intracellular trafficking pathway that occurs in nutrient uptake, signal transduction and reconstruction of cell polarity and is conserved in eukaryotic cells. In fungi, endocytosis plays crucial roles in the physiology of hyphal growth and pathogenicity. vidence for endocytosis in filamentous fungi is detected by the ...
    Precision Technique for Splenectomy Limits Mouse Stress Responses for Accurate and Realistic Measurements for Investigating Inflammation and Immunity
    脾切除术限制小鼠应激反应的精准技术用于精确现实检测炎症和免疫
    作者:Shengwen Calvin Li, Anthony D. Rangel and Mustafa H. Kabeer日期:08/05/2019,浏览量:241,Q&A: 0
    [Abstract] Splenectomy in an animal model requires a standardized technique utilizing best practice to avoid variability which can result in adverse impact to the animal resulting in flawed physiologic responses simply due to technique rather than to the studied variables. In the case of the spleen, often investigators are analyzing the animal immune or ...