微生物学

分类

    现刊
    CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
    诺氏疟原虫CRISPR-Cas9基因编辑
    作者:Franziska Mohring, Melissa N. Hart, Avnish Patel, David A. Baker and Robert W. Moon日期:02/20/2020,浏览量:126,Q&A: 0
    Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. ...
    CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
    诺氏疟原虫CRISPR-Cas9基因编辑
    作者:Franziska Mohring, Melissa N. Hart, Avnish Patel, David A. Baker and Robert W. Moon日期:02/20/2020,浏览量:126,Q&A: 0
    [Abstract] Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. falciparum, P. knowlesi is the only other malaria parasite capable of being maintained in ...
    Transcervical Mouse Infections with Chlamydia trachomatis and Determination of Bacterial Burden
    小鼠宫颈沙眼衣原体感染及细菌负荷测定
    作者:Karthika Rajeeve and Rajeeve Sivadasan日期:02/05/2020,浏览量:226,Q&A: 0
    [Abstract] Chlamydia trachomatis is an obligate human pathogen. It infects the genital tract of humans ascending into the fallopian tube, exacerbated by chronic pelvic pain, pelvic inflammatory disease, and fallopian tube scaring resulting in infertility and other malignancies. The major hurdle in controlling chlamydial spread is that the ...
    Rapid Detection of Proliferative Bacteria by Electrical Stimulation
    利用电刺激快速检测细菌增殖
    作者:Conor LA Edwards, Dmitry Malyshev, James P Stratford and Munehiro Asally日期:02/05/2020,浏览量:633,Q&A: 0
    [Abstract] Detecting live bacteria is an important task for antimicrobial susceptibility testing (AST) in the medical sector and for quality-monitoring in biological industries. Current methods for live-bacteria detection suffer limitations in speed or sensitivity. In a recent paper, we reported that electrical response dynamics in membrane potential enable ...
    Expression and Purification of Adeno-associated Virus Virus-like Particles in a Baculovirus System and AAVR Ectodomain Constructs in E. coli
    腺相关病毒样颗粒在杆状病毒系统中的表达纯化及在大肠杆菌AAVR胞外区的构建
    作者:Nancy Meyer, Omar Davulcu, Qing Xie, Mark Silveria, Grant M. Zane, Edward Large and Michael S. Chapman日期:02/05/2020,浏览量:242,Q&A: 0
    [Abstract] Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape. Presented here are protocols for the production of: (a) the outer protein shells (virus-like particles or VLPs) for serotype 2 ...
    Competition Assays to Quantify the Effect of Biocontrol Yeasts against Plant Pathogenic Fungi on Fruits
    竞争分析以量化酵母菌对水果上植物病原真菌的生防作用
    作者:Electine Magoye, Melanie Pfister, Maja Hilber-Bodmer and Florian M. Freimoser日期:02/05/2020,浏览量:236,Q&A: 0
    [Abstract] Yeasts such as Aureobasidium pullulans are unicellular fungi that occur in all environments and play important roles in biotechnology, medicine, food and beverage production, research, and agriculture. In the latter, yeasts are explored as biocontrol agents for the control of plant pathogenic fungi (e.g., Botrytis cinerea, ...
    Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil
    硫酸酯酶法测定植物对土壤微生物活性的影响
    作者:Anna Koprivova, Achim Schmalenberger and Stanislav Kopriva日期:01/20/2020,浏览量:521,Q&A: 0
    [Abstract] Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various Arabidopsis genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew ...
    Quantifying HIV-1-Mediated Gut CD4+ T Cell Death in the Lamina Propria Aggregate Culture (LPAC) Model
    固有层聚集培养模式中HIV-1介导的Gut CD4+ T细胞凋亡的量化分析
    作者:Stephanie M. Dillon, Kejun Guo, Moriah J. Castleman, Mario L. Santiago and Cara C. Wilson日期:01/20/2020,浏览量:327,Q&A: 0
    [Abstract] Gut CD4 T cells are major targets of HIV-1 and are massively depleted early during infection. To better understand the mechanisms governing HIV-1-mediated CD4 T cell death, we developed the physiologically-relevant Lamina Propria Aggregate Culture (LPAC) model. The LPAC model is ideal for studying CD4 T cell death induced by clinically-relevant ...
    A Radioactive-free Kinase Inhibitor Discovery Assay Against the Trypanosoma brucei Glycogen Synthase Kinase-3 short (TbGSK-3s)
    一种无放射性布氏锥虫糖原合成酶激酶-3抑制剂探究实验
    作者:Antonia Efstathiou and Despina Smirlis日期:01/20/2020,浏览量:188,Q&A: 0
    [Abstract] The identification of small molecules possessing inhibitory activity in vitro, against a given target kinase, is the first step in the drug discovery process. Herein, we describe a non radioactive protocol using luciferase-based ATP assay for the identification of inhibitors for the short isoform of the Trypanosoma brucei’s ...
    Study of Microbial Extracellular Vesicles: Separation by Density Gradients, Protection Assays and Labelling for Live Tracking
    微生物胞外囊泡的研究:密度梯度分离,保护性分析和活体示踪标记
    作者:Carolina Coelho, Raghav Vij, Daniel Q. Smith, Nathan R. Brady, Anne Hamacher-Brady and Arturo Casadevall日期:01/20/2020,浏览量:353,Q&A: 0
    [Abstract] Extracellular vesicles (EVs) are produced by all domains of life including Bacteria, Archaea and Eukarya. EVs are critical for cellular physiology and contain varied cargo: virulence factors, cell wall remodeling enzymes, extracellular matrix components and even nucleic acids and metabolites. While various protocols for isolating EVs have been ...
    Measurement of the Promoter Activity in Escherichia coli by Using a Luciferase Reporter
    利用荧光素酶报告系统检测大肠杆菌中启动子活性
    作者:Yuki Yamanaka, Hiroki Watanabe, Erika Yamauchi, Yukari Miyake and Kaneyoshi Yamamoto日期:01/20/2020,浏览量:312,Q&A: 0
    [Abstract] The reporter system is widely used technique for measuring promoter activity in bacterial cells. Until now, a number of reporter system have been developed, but the bioluminescent reporter constructed from the bacterial luciferase genes is one of the useful systems for measuring in vivo dynamics of gene expression. The introduced ...