微生物学

分类

    现刊
    Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
    农杆菌介导的优良籼稻品种Komboka的高效转化
    Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of ...
    An in vitro DNA Sensor-based Assay to Measure Receptor-specific Adhesion Forces of Eukaryotic Cells and Pathogens
    基于DNA传感器的真核细胞与病原体受体特异性粘附力的体外检测
    作者:Maurizio Wack, Tina Wiegand, Friedrich Frischknecht and E. Ada Cavalcanti-Adam日期:09/05/2020,浏览量:716,Q&A: 0
    Motility of eukaryotic cells or pathogens within tissues is mediated by the turnover of specific interactions with other cells or with the extracellular matrix. Biophysical characterization of these ligand-receptor adhesions helps to unravel the ...
    Live-cell Imaging by Super-resolution Confocal Live Imaging Microscopy (SCLIM): Simultaneous Three-color and Four-dimensional Live Cell Imaging with High Space and Time Resolution
    超分辨率共聚焦显微镜活体细胞成像(SCLIM):高时空分辨率的三色、四维同步活体细胞成像
    作者:Kazuo Kurokawa and Akihiko Nakano日期:09/05/2020,浏览量:674,Q&A: 0
    Many questions in cell biology can be solved by state-of-the-art technology of live cell imaging. One good example is the mechanism of membrane traffic, in which small membrane carriers are rapidly moving around in the cytoplasm to deliver cargo ...
    Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus
    人单纯疱疹病毒CRISPR/Cas9抑制作用的筛选方法
    The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA ...
    A High-throughput Interbacterial Competition Platform
    一个高通量细菌间竞争平台
    作者:Hsiao-Han Lin and Erh-Min Lai日期:09/05/2020,浏览量:659,Q&A: 0
    Contact-dependent interbacterial competition is a common strategy used by bacteria to fight for their ecological niches. Interbacterial competition is monitored by a competition assay involving co-culturing the attacker and the recipient bacterial ...
    Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves
    1型单纯疱疹病毒的繁殖,滴定和一步生长曲线
    Given the endemic seroprevalence of herpes simplex viruses (HSV), its associated human diseases, and the emergence of acyclovir-resistant strains, there is a continuous need for better antiviral therapies. Towards this aim, identifying mechanistic ...
    Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
    农杆菌介导的优良籼稻品种Komboka的高效转化
    [Abstract] Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of the world’s population. As a result, numerous transformation methods have been developed for both ...
    An in vitro DNA Sensor-based Assay to Measure Receptor-specific Adhesion Forces of Eukaryotic Cells and Pathogens
    基于DNA传感器的真核细胞与病原体受体特异性粘附力的体外检测
    作者:Maurizio Wack, Tina Wiegand, Friedrich Frischknecht and E. Ada Cavalcanti-Adam日期:09/05/2020,浏览量:716,Q&A: 0
    [Abstract] Motility of eukaryotic cells or pathogens within tissues is mediated by the turnover of specific interactions with other cells or with the extracellular matrix. Biophysical characterization of these ligand-receptor adhesions helps to unravel the molecular mechanisms driving migration. Traction force microscopy or optical tweezers are typically ...
    Live-cell Imaging by Super-resolution Confocal Live Imaging Microscopy (SCLIM): Simultaneous Three-color and Four-dimensional Live Cell Imaging with High Space and Time Resolution
    超分辨率共聚焦显微镜活体细胞成像(SCLIM):高时空分辨率的三色、四维同步活体细胞成像
    作者:Kazuo Kurokawa and Akihiko Nakano日期:09/05/2020,浏览量:674,Q&A: 0
    [Abstract] Many questions in cell biology can be solved by state-of-the-art technology of live cell imaging. One good example is the mechanism of membrane traffic, in which small membrane carriers are rapidly moving around in the cytoplasm to deliver cargo proteins between organelles. For directly visualizing the events in membrane trafficking system, ...
    Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus
    人单纯疱疹病毒CRISPR/Cas9抑制作用的筛选方法
    [Abstract] The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA efficiency by altering accessibility of genomic DNA through DNA modifications such as epigenetic marks and ...
    A High-throughput Interbacterial Competition Platform
    一个高通量细菌间竞争平台
    作者:Hsiao-Han Lin and Erh-Min Lai日期:09/05/2020,浏览量:659,Q&A: 0
    [Abstract] Contact-dependent interbacterial competition is a common strategy used by bacteria to fight for their ecological niches. Interbacterial competition is monitored by a competition assay involving co-culturing the attacker and the recipient bacterial cells on agar, followed by recovery of the surviving recipient cells. Conventional interbacterial ...
    In vitro Cultivation and Visualization of Malaria Liver Stages in Primary Simian Hepatocytes
    猴原代肝细胞体外培养及疟疾肝期观察
    作者:Devendra Kumar Gupta and Thierry Diagana日期:08/20/2020,浏览量:579,Q&A: 0
    [Abstract] Human liver is the primary and obligatory site for malaria infection where sporozoites invade host hepatocytes. Malaria hepatic stages are asymptomatic and represent an attractive target for development of anti-malarial interventions and vaccines. However, owing to lack of robust and reproducible in vitro culture system, it is difficult ...
    Candida albicans Agar Invasion Assays
    白念珠菌琼脂侵入实验
    作者:Shamoon Naseem, Lois M. Douglas and James B. Konopka日期:08/20/2020,浏览量:588,Q&A: 0
    [Abstract] The ability of the human fungal pathogen Candida albicans to disseminate into tissues is promoted by a switch from budding to invasive hyphal growth. This morphological transition is stimulated by multiple environmental factors that can vary at different sites of infection. To identify genes that promote invasive growth, C. albicans ...
    Neutrophil Extracellular Trap Killing Assay of Candida albicans
    白念珠菌中性粒细胞胞外诱杀试验
    作者:Sheng-Yang Wu and Betty A. Wu-Hsieh日期:08/20/2020,浏览量:698,Q&A: 0
    [Abstract] Fungal pathogen Candida albicans is one of the top leading causes of overall healthcare-associated bloodstream infections worldwide. Neutrophil is the major effector cell to clear C. albicans infection. Our study showed that mouse neutrophils utilize two independent mechanisms to kill C. albicans: one is CR3 downstream ...
    Determination of the Cellular Ion Concentration in Saccharomyces cerevisiae Using ICP-AES
    ICP-AES法测定酿酒酵母细胞离子浓度
    作者:Louise Thines, Anne Iserentant and Pierre Morsomme日期:08/20/2020,浏览量:592,Q&A: 0
    [Abstract] The yeast Saccharomyces cerevisiae has been perceived over decades as a highly valuable model organism for the investigation of ion homeostasis. Indeed, many of the genes and biological systems that function in yeast ion homeostasis are conserved throughout unicellular eukaryotes to humans. In this context, measurement of the yeast ...
    Paper Lateral Flow Biosensor for Nodavirus Reverse Transcribed RNA Detection
    用于诺达病毒逆转录RNA检测的纸侧向流生物传感器
    作者:Dimitra K. Toubanaki and Evdokia Karagouni日期:08/05/2020,浏览量:390,Q&A: 0
    [Abstract] Paper nanobiosensors have been established as an excellent platform for analysis of veterinary and human pathogens causing various diseases. Especially, lateral flow assays or biosensors ideal for sensitive, rapid, robust and accurate analysis in laboratory setups and on-site analysis. Viral RNA detection is of great importance for public health ...