微生物学

分类

    现刊
    Observing Nutrient Gradients, Gene Expression and Growth Variation Using the "Yeast Machine" Microfluidic Device
    使用“酵母机”微流控设备观察营养梯度,基因表达和生长变异
    The natural environment of microbial cells like bacteria and yeast is often a complex community in which growth and internal organization reflect morphogenetic processes and interactions that are dependent on spatial position and time. While most of ...
    Microscopy-based Methods for Rosetting Assay in Malaria Research
    基于显微技术的疟疾玫瑰花环试验
    作者:Wenn-Chyau Lee and Laurent Rénia日期:07/05/2020,浏览量:82,Q&A: 0
    In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune ...
    Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)
    叠氮苯酰溴(APB)定位蛋白质结合方向的DNA图谱
    作者:Himasha M. Perera and Michael A. Trakselis日期:06/20/2020,浏览量:638,Q&A: 0
    The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action. As an example, in a replisome, the orientation of the DNA helicase at the replication ...
    Maintenance and Quantitative Phenotyping of the Oomycete-plant Model Pathosystem Hyaloperonospora arabidopsidisArabidopsis
    卵菌-植物模型病理系统(活体营养型卵菌–拟南芥)的维持和定量表型分析
    作者:Fang-Yu Hwu and Martin Parniske日期:06/20/2020,浏览量:455,Q&A: 0
    The interaction between the host plant Arabidopsis thaliana (Arabidopsis) and the oomycete Hyaloperonospora arabidopsidis (Hpa) is an established model system for the study of an obligate biotrophic downy mildew ...
    Identification of Buffer Conditions for Optimal Thermostability and Solubility of Herpesviral Protein UL37 Using the Thermofluor Assay
    用热荧光法鉴定疱疹病毒蛋白UL37的最佳热稳定性和溶解度的缓冲液条件
    作者:Andrea L. Koenigsberg, Jared D. Pitts and Ekaterina E. Heldwein日期:06/20/2020,浏览量:322,Q&A: 0
    Structural and biochemical studies of proteins require high amounts of stable, purified proteins. Protein stability often depends on the buffer composition, which includes pH and concentration of salts or other solutes such as glycerol, hence an ...
    Genomic Edition of Ashbya gossypii Using One-vector CRISPR/Cas9
    利用一个CRISPR/Cas9载体对棉囊阿舒氏酵母进行基因组编辑
    作者:Gloria Muñoz-Fernández, Alberto Jiménez and José Luis Revuelta日期:06/20/2020,浏览量:388,Q&A: 0
    The CRISPR/Cas9 system is a novel genetic tool which allows the precise manipulation of virtually any genomic sequence. In this protocol, we use a specific CRISPR/Cas9 system for the manipulation of Ashbya gossypii. The filamentous fungus ...
    Live Cell Measurement of the Intracellular pH of Yeast by Flow Cytometry Using a Genetically-Encoded Fluorescent Reporter
    一种基于流式细胞术的利用基因编码荧光报告测定酵母活细胞内pH值的方法
    作者:Catherine G. Triandafillou and D. Allan Drummond日期:06/20/2020,浏览量:491,Q&A: 0
    The intracellular pH of yeast is a tightly regulated physiological cue that changes in response to growth state and environmental conditions. Fluorescent reporters, which have altered fluorescence in response to local pH changes, can be used to ...
    Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves
    1型单纯疱疹病毒的繁殖,滴定和一步生长曲线
    Given the endemic seroprevalence of herpes simplex viruses (HSV), its associated human diseases, and the emergence of acyclovir-resistant strains, there is a continuous need for better antiviral therapies. Towards this aim, identifying mechanistic ...
    Observing Nutrient Gradients, Gene Expression and Growth Variation Using the "Yeast Machine" Microfluidic Device
    使用“酵母机”微流控设备观察营养梯度,基因表达和生长变异
    [Abstract] The natural environment of microbial cells like bacteria and yeast is often a complex community in which growth and internal organization reflect morphogenetic processes and interactions that are dependent on spatial position and time. While most of research is performed in simple homogeneous environments (e.g., bulk liquid cultures), ...
    Microscopy-based Methods for Rosetting Assay in Malaria Research
    基于显微技术的疟疾玫瑰花环试验
    作者:Wenn-Chyau Lee and Laurent Rénia日期:07/05/2020,浏览量:82,Q&A: 0
    [Abstract] In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune escape mechanism for the parasite. However, it has been associated with malaria pathogenesis, possibly ...
    Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)
    叠氮苯酰溴(APB)定位蛋白质结合方向的DNA图谱
    作者:Himasha M. Perera and Michael A. Trakselis日期:06/20/2020,浏览量:638,Q&A: 0
    [Abstract] The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action. As an example, in a replisome, the orientation of the DNA helicase at the replication fork directs the assembly of the other associated replisome proteins. We have recently determined ...
    Maintenance and Quantitative Phenotyping of the Oomycete-plant Model Pathosystem Hyaloperonospora arabidopsidisArabidopsis
    卵菌-植物模型病理系统(活体营养型卵菌–拟南芥)的维持和定量表型分析
    作者:Fang-Yu Hwu and Martin Parniske日期:06/20/2020,浏览量:455,Q&A: 0
    [Abstract] The interaction between the host plant Arabidopsis thaliana (Arabidopsis) and the oomycete Hyaloperonospora arabidopsidis (Hpa) is an established model system for the study of an obligate biotrophic downy mildew interaction. The evaluation of the developmental success of Hpa is often based on the quantification ...
    Identification of Buffer Conditions for Optimal Thermostability and Solubility of Herpesviral Protein UL37 Using the Thermofluor Assay
    用热荧光法鉴定疱疹病毒蛋白UL37的最佳热稳定性和溶解度的缓冲液条件
    作者:Andrea L. Koenigsberg, Jared D. Pitts and Ekaterina E. Heldwein日期:06/20/2020,浏览量:322,Q&A: 0
    [Abstract] Structural and biochemical studies of proteins require high amounts of stable, purified proteins. Protein stability often depends on the buffer composition, which includes pH and concentration of salts or other solutes such as glycerol, hence an efficient method for identifying optimal buffer conditions for stability would minimize time and ...
    Genomic Edition of Ashbya gossypii Using One-vector CRISPR/Cas9
    利用一个CRISPR/Cas9载体对棉囊阿舒氏酵母进行基因组编辑
    作者:Gloria Muñoz-Fernández, Alberto Jiménez and José Luis Revuelta日期:06/20/2020,浏览量:388,Q&A: 0
    [Abstract] The CRISPR/Cas9 system is a novel genetic tool which allows the precise manipulation of virtually any genomic sequence. In this protocol, we use a specific CRISPR/Cas9 system for the manipulation of Ashbya gossypii. The filamentous fungus A. gossypii is currently used for the industrial production of riboflavin (vitamina B2). In ...
    Live Cell Measurement of the Intracellular pH of Yeast by Flow Cytometry Using a Genetically-Encoded Fluorescent Reporter
    一种基于流式细胞术的利用基因编码荧光报告测定酵母活细胞内pH值的方法
    作者:Catherine G. Triandafillou and D. Allan Drummond日期:06/20/2020,浏览量:491,Q&A: 0
    [Abstract] The intracellular pH of yeast is a tightly regulated physiological cue that changes in response to growth state and environmental conditions. Fluorescent reporters, which have altered fluorescence in response to local pH changes, can be used to measure intracellular pH. While microscopy is often used to make such measurements, it is relatively ...
    Differential Fractionation of Erythrocytes Infected by Plasmodium berghei
    感染伯氏疟原虫红细胞的差异分离
    作者:Bénédicte Gnangnon, Véronique Peucelle and Christine Pierrot日期:06/05/2020,浏览量:374,Q&A: 0
    [Abstract] The study of host/pathogen interactions at the cellular level during Plasmodium intra-erythrocytic cycle requires differential extraction techniques aiming to analyze the different compartments of the infected cell. Various protocols have been proposed in the literature to study specific compartments and/or membranes in the infected ...
    RNA Extraction from Ears and Draining Lymph Nodes of Mice Infected with Leishmania amazonensis
    亚马逊利什曼原虫感染小鼠耳及引流淋巴结RNA的提取
    作者:Emilie Giraud and Evie Melanitou日期:06/05/2020,浏览量:456,Q&A: 0
    [Abstract] Parasites of the genus Leishmania infect the mammalian hosts, including mice and humans and cause cutaneous or visceral leishmaniasis depending upon the parasite species transmitted by the vector sandfly. Leishmania amazonensis is one of the Leishmania species responsible for the cutaneous form of the disease. We have ...
    Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions
    适于研究树-真菌互作的安全DNA提取方法
    作者:Susanna Keriö, Eeva Terhonen and Jared M. LeBoldus日期:06/05/2020,浏览量:964,Q&A: 0
    [Abstract] We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples and from mycelium grown on solid and liquid media. DNA was extracted from juvenile and mature host ...