哺乳纲

分类

    现刊
    Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes
    大鼠肾脏单纯线粒体的分离和线粒体呼吸链复合物蛋白质免疫印迹
    Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell ...
    Isolation and Transcriptomic Profiling of Single Myofibers from Mice
    小鼠单根肌纤维的分离和转录组分析
    Skeletal muscle is composed of different cells and myofiber types, with distinct metabolic and structural features. Generally, transcriptomic analysis of skeletal muscle is performed using whole muscle, resulting in average information as all cells ...
    Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices
    反义寡脱氧核苷酸灌注可阻突触可塑性相关蛋白在海马切片上的基因表达且无诱导补偿作用
    The elucidation of the molecular mechanisms of long-term synaptic plasticity has been hindered by both the compensation that can occur after chronic loss of the core plasticity molecules and by ex vivo conditions that may not reproduce in ...
    ATAC-seq on Sorted Adult Mouse Neurons
    ATAC-seq用于分选成熟小鼠神经元
    作者:Erin A. Clark, Yasuyuki Shima and Sacha Nelson日期:10/05/2019,浏览量:186,Q&A: 0
    Transcription regulation is a key aspect of cellular identity established during development and maintained into adulthood. Molecular and biochemical assays that probe the genome are critical tools in exploring mechanisms of transcription regulation ...
    3D Organoid Formation from the Murine Salivary Gland Cell Line SIMS
    小鼠唾液腺SIMS细胞系的三维类器官形成
    作者:Harleen K. Athwal and Isabelle M. A. Lombaert日期:10/05/2019,浏览量:148,Q&A: 0
    Salivary glands consist of multiple phenotypically and functionally unique cell populations, such as the acinar, ductal, and myoepithelial cells that help produce, modify, and secrete saliva (Lombaert et al., 2011). Identification of ...
    An Operant Conditioning Task to Assess the Choice between Wheel Running and Palatable Food in Mice
    一项小鼠在转轮装置和可口食物之间选择的操作式条件反射测试
    作者:Bastien Redon, Imane Hurel, Giovanni Marsicano and Francis Chaouloff日期:10/05/2019,浏览量:219,Q&A: 0
    Wheel running, especially in the homecage, has been widely used to study the neurobiology of exercise because animal tends to use it voluntarily. However, as for each reward, its consumption (in the present case, running performance) does not ...
    Reconstituting Breast Tissue with Organotypic Three-dimensional Co-culture of Epithelial and Stromal Cells in Discontinuous Extracellular Matrices
    通过不连续细胞外基质中上皮细胞和基质细胞三维共培养重建乳腺组织
    作者:Gang Ren, Vandana Sharma, Joshua Letson, Yashna Walia, Veani Fernando and Saori Furuta日期:10/05/2019,浏览量:209,Q&A: 0
    Co-culture systems utilizing reconstituted or synthetic extracellular matrix (ECM) and micropatterning techniques have enabled the reconstruction of surface epithelial tissues. This technique has been utilized in the regeneration, disease modeling ...
    Imaging the Vasculature of Immunodeficient Mice Using Positron Emission Tomography/Computed Tomography (PET/CT) and 18F-fluorodeoxyglucose Labeled Human Erythrocytes
    利用正电子发射断层扫描/计算机断层扫描(PET、CT)和18F-氟脱氧葡萄糖标记人类红细胞 进行免疫缺陷小鼠脉管系统成像
    作者:Shaowei Wang and Jung W. Choi日期:10/05/2019,浏览量:109,Q&A: 0
    Nuclear blood pool imaging using radiolabeled red blood cells has been used in the clinical setting for the evaluation of a number of medical conditions including gastrointestinal hemorrhage, impaired cardiac contractility, and altered ...
    A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-based Scaffolds (3D-dCBS)
    一种获得去细胞牛脊髓细胞外基质构成支架的新方法
    作者:Yavuz E. Arslan, Burcu Efe and Tugba Sezgin Arslan日期:10/05/2019,浏览量:145,Q&A: 0
    Extracellular matrix (ECM)-based tissue engineering scaffolds have an essential role in promoting tissue regeneration. Nerve tissue engineering aims at facilitating the repair of permanent damage to the peripheral and central nervous systems, which ...
    Simple Protocol for Distinguishing Drug-induced Effects on Spatial Memory Acquisition, Consolidation and Retrieval in Mice Using the Morris Water Maze
    一种利用Morris水迷宫分辨药物诱导对于小鼠空间记忆获取、巩固和恢复学习行为影响的简单方案
    作者:Ariel K. Frame, Asad Lone, Richard A. Harris and Robert C. Cumming日期:09/20/2019,浏览量:206,Q&A: 0
    The Morris water maze (MWM) is one of the most commonly used tests for assessing spatial learning and memory in mice. While the MWM is highly amenable to testing the effects of memory modifying drugs, most studies do not consider the timing or ...
    Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal Mouse Macrophages by Flow Cytometry
    利用流式细胞术对巯基乙醇酸盐诱导的小鼠腹腔巨噬细胞中LPS-TLR4复合物内化的定量分析
    作者:Katharina Richard, Stefanie N. Vogel and Darren J. Perkins日期:09/20/2019,浏览量:207,Q&A: 0
    Internalization of cell surface Toll-like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using ...
    Conjugation of Fab’ Fragments with Fluorescent Dyes for Single-molecule Tracking on Live Cells
    Fab片段结合荧光染料用于活细胞单分子示踪
    作者:I-Ting Teng, Xiangning Bu and Inhee Chung日期:09/20/2019,浏览量:241,Q&A: 0
    Our understanding of the regulation and functions of cell-surface proteins has progressed rapidly with the advent of advanced optical imaging techniques. In particular, single-molecule tracking (SMT) using bright fluorophores conjugated to ...
    Immunohistochemical Staining of CD8α in Diabetic Mouse Kidney
    糖尿病小鼠肾脏CD8α的免疫组化染色
    作者:Ming-Zhi Zhang and Raymond C. Harris日期:09/20/2019,浏览量:197,Q&A: 0
    Immune cell infiltration, particularly cytotoxic CD8α lymphocyte infiltration, plays an important role in development of diabetic nephropathy. Although CD8α infiltration can be evaluated by its production of cytokines, its localization in the kidney ...
    SIRF: A Single-cell Assay for in situ Protein Interaction with Nascent DNA Replication Forks
    SIRF:一种蛋白与新生DNA复制叉原位相互作用的单细胞检测办法
    作者:Sunetra Roy and Katharina Schlacher日期:09/20/2019,浏览量:300,Q&A: 0
    The duplication of DNA is a fundamental process that is required for the transfer of the genetic information from parent to daughter cells. Aberrant DNA replication processes are associated with diverse disease phenotypes, including developmental ...
    Subcellular Fractionation of Hela Cells for Lysosome Enrichment Using a Continuous Percoll-density Gradient
    利用连续percoll密度梯度富集hela细胞溶酶体亚细胞组分
    作者:Julian M. Carosi, Kathryn J. Hattersley, Yi Cui, Zhe Yang, Rohan D. Teasdale and Timothy J. Sargeant日期:09/20/2019,浏览量:351,Q&A: 0
    The enrichment of lysosomes is a useful way to study their structure and function. These dynamic vesicles can be enriched from cell cultures in a variety of ways including immunoprecipitation and fluorescence-activated organelle sorting. These ...
    Supported Cell Membrane Sheets to Monitor Protein Assembly
    蛋白质组装的细胞膜片监测
    作者:Simon Erlendsson, Thor Seneca Thorsen and Kenneth Lindegaard Madsen日期:09/20/2019,浏览量:251,Q&A: 0
    Studying protein-protein and protein-lipid interactions in their native environment is highly desirable, yet, the heterogeneity and complexity of cellular systems limits the repertoire of experimental methods available. In cells, interactions are ...
    Characterization of Biological Motion Using Motion Sensing Superpixels
    基于运动传感超像素的生物运动特性研究
    作者:Felix Y. Zhou, Carlos Ruiz-Puig, Richard P. Owen, Michael J. White, Jens Rittscher and Xin Lu日期:09/20/2019,浏览量:239,Q&A: 0
    Precise spatiotemporal regulation is the foundation for the healthy development and maintenance of living organisms. All cells must correctly execute their function in the right place at the right time. Cellular motion is thus an important dynamic ...
    Sciatic Nerve Cut and Repair Using Fibrin Glue in Adult Mice
    纤维蛋白胶在成年小鼠坐骨神经损伤修复中的应用
    作者:Erica T. Akhter, Travis M. Rotterman, Arthur W. English and Francisco J. Alvarez日期:09/20/2019,浏览量:211,Q&A: 0
    Peripheral nerve injury (PNI) is an excellent model for studying neural responses to injury and elucidating the mechanisms that can facilitate axon regeneration. As such, several animal models have been employed to study regenerative mechanisms ...
    Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation
    小鼠胚胎脊髓的组织块培养和体外电穿孔介导的基因转移
    Developing axons change responsiveness to guidance cues during the journey to synapse with target cells. Axon crossing at the ventral midline serves as a model for studying how axons accomplish such a switch in their response. Although primary ...
    A Cell Culture Model that Mimics Physiological Tissue Oxygenation Using Oxygen-permeable Membranes
    一种利用透氧膜模拟生理组织氧合的细胞培养模式
    作者:Chloe-Anne Martinez, Peter A. Cistulli and Kristina M. Cook日期:09/20/2019,浏览量:293,Q&A: 0
    Dissolved oxygen and its availability to cells in culture is an overlooked variable which can have significant consequences on experimental research outcomes, including reproducibility. Oxygen sensing pathways play key roles in cell growth and ...
    A High-throughput and Pathophysiologically Relevant Astrocyte-motor Neuron Co-culture Assay for Amyotrophic Lateral Sclerosis Therapeutic Discovery
    高通量病理生理相关星形胶质细胞运动神经元共培养技术在肌萎缩侧索硬化治疗中的应用
    作者:Matthew J. Stopford, Scott P. Allen and Laura Ferraiuolo日期:09/05/2019,浏览量:621,Q&A: 0
    Amyotrophic lateral sclerosis (ALS) is an adult onset neurological disorder characterized by loss of motor neurons leading to progressive muscle wasting and eventually death. Astrocytes play a key role in disease pathogenesis. However, the ability ...
    A Refined Protocol for Identifying Citrulline-specific Monoclonal Antibodies from Single Human B Cells from Rheumatoid Arthritis Patient Material
    类风湿性关节炎患者单个B细胞中瓜氨酸特异单克隆抗体的改进鉴定方案
    We describe here a detailed, refined protocol for the generation of citrulline-specific monoclonal antibodies from single human B cells from rheumatoid arthritis (RA) patients. This protocol provides a detailed guide of the procedure starting from ...
    Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells
    人干细胞源性神经元与少突胶质祖细胞的共培养
    作者:Stephanie Dooves, Aishwarya G. Nadadhur, Lisa Gasparotto and Vivi M. Heine日期:09/05/2019,浏览量:575,Q&A: 0
    Crosstalk between neurons and oligodendrocytes is important for proper brain functioning. Multiple co-culture methods have been developed to study oligodendrocyte maturation, myelination or the effect of oligodendrocytes on neurons. However, most of ...
    A Robust, One-step FRET Assay for Human Heparanase
    人乙酰肝素酶的一步快速FRET检测
    作者:Jyothi C. Sistla and Umesh R. Desai日期:09/05/2019,浏览量:296,Q&A: 0
    Heparanase, an endo-β-D-glucuronidase, cleaves cell surface and extracellular matrix heparan sulfate (HS) chains at distinct sites and plays important biological roles including modulation of cell growth and metastasis. Although a number of ...
    Photoaffinity Labeling of Respiratory Complex I in Bovine Heart Submitochondrial Particles by Photoreactive [125I] amilorides
    利用光敏阿米洛利光亲和标记牛心脏亚线粒体颗粒中呼吸链复合物I
    作者:Masatoshi Murai and Hideto Miyoshi日期:09/05/2019,浏览量:257,Q&A: 0
    The architecture of quinone/inhibitor-access channel in proton-translocating NADH-quinone oxidoreductase (respiratory complex I) was modeled by X-ray crystallography and cryo-EM, however, it remains debatable whether the channel model reflects the ...
    Isolation of Neutrophil Nuclei for Use in NETosis Assays
    分离中性粒细胞核用于 NETosis分析
    作者:Dave Boucher日期:09/05/2019,浏览量:424,Q&A: 0
    Neutrophils are critical immune cells that protect our body against invading pathogens. They generate antibacterial DNA structures called neutrophil extracellular traps (NET). Recently we identified a new mechanism that enables NET formation. We ...
    Estrous Cycle Monitoring in Mice with Rapid Data Visualization and Analysis
    利用快速数据可视化分析进行小鼠发情周期监测
    作者:Leanna K. Pantier, Jiang Li and Catherine A. Christian日期:09/05/2019,浏览量:550,Q&A: 0
    The estrous cycle provides a readout of reproductive health in female laboratory rodents, and estrous cycle stage can be an important physiological variable. Accurate assessment of estrous cycle stage is also important in producing timed pregnancies ...
    A Novel Dual Lentiviral CRISPR-based Transcriptional Activation System for Gene Expression Regulation in Neurons
    一种新型基于CAS9双载体慢病毒转录激活系统的神经组织基因表达调控
    作者:Katherine E. Savell, Faraz A. Sultan and Jeremy J. Day日期:09/05/2019,浏览量:708,Q&A: 0
    Robust and efficient gene expression control enables the study of a gene’s function in the central nervous system. Advances in CRISPR-based technology provide new avenues not only for gene editing, but for complex transcriptional control. Here, we ...
    Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes
    一种改良的Smart-seq2样品制备方案在小鼠卵母细胞中应用稀有细胞全长单细胞mRNA测序
    作者:Rebecca S. Treger, Scott D. Pope, Xiaojun Xing and Akiko Iwasaki日期:08/20/2019,浏览量:439,Q&A: 0
    Endogenous retroviruses (ERV) are transposable retroelements that form ~10% of the murine genome and whose family members are differentially expressed throughout embryogenesis. However, precise regulation of ERV in germ cells remains unclear. To ...
    In vitro Differentiation of Thymic Treg Cell Progenitors to Mature Thymic Treg Cells
    体外胸腺调节性T细胞祖细胞向成熟胸腺调节性T细胞的分化
    作者:David L. Owen and Michael A. Farrar日期:08/20/2019,浏览量:318,Q&A: 0
    Thymic Treg cell differentiation occurs via a two-step process. Step one generates Treg cell progenitors (TregP) and is driven by strong TCR interactions with antigens presented in the thymus. Step two is initiated ...
    Fibroblast Gap-closure Assay-Microscopy-based in vitro Assay Measuring the Migration of Murine Fibroblasts
    基于成纤维细胞间隙封闭显微成像的鼠成纤维细胞的体外迁移分析
    作者:Agnieszka P. Looney and Mallar Bhattacharya日期:08/20/2019,浏览量:519,Q&A: 0
    Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts.

    ...
    Construction of Viral Vectors for Cell Type-specific CRISPR Gene Editing in the Adult Mouse Brain
    用于成年小鼠脑组织的细胞特异性CRISPR基因编辑的病毒载体的构建
    作者:Hiroshi Yamaguchi and Luis de Lecea日期:08/20/2019,浏览量:576,Q&A: 0
    Recently developed gene editing technologies based on engineered CRISPR/Cas9 systems enables researchers to disrupt genes in a cell type-specific manner in the adult mouse brain. Using these technologies, we recently showed that the dopamine ...
    A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
    利用改进的Sca/eS和机器学习用于螺旋器中毛细胞的成像和分析
    Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and ...
    Cell-based Assay for Recruitment of DDR1 to Collagen-coated Beads
    以细胞为基础的DDR1向胶原包被微珠的招募分析
    作者:Victoria Juskaite and Birgit Leitinger日期:08/20/2019,浏览量:352,Q&A: 0
    The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for ...
    Three-dimensional Reconstruction and Quantification of Proteins and mRNAs at the Single-cell Level in Cultured Cells
    培养细胞中蛋白和mRNA在单细胞水平上的三维重构和量化分析
    作者:Xueer Jiang, Ingrid Brust-Mascher and Li-En Jao日期:08/20/2019,浏览量:649,Q&A: 0
    Gene expression is often regulated by the abundance, localization, and translation of mRNAs in both space and time. Being able to visualize mRNAs and protein products in single cells is critical to understand this regulatory process. The development ...
    Cylinder Test to Assess Sensory-motor Function in a Mouse Model of Parkinson’s Disease
    基于圆筒试验的小鼠帕金森综合症模型中感觉运动系统功能测试
    Parkinson’s disease is a progressive neurodegenerative movement disorder that happens due to the loss of dopaminergic neurons in the substantia nigra. The deficiency of dopamine in the basal nuclei drives cardinal motor symptoms such as bradykinesia ...
    Whisker Nuisance Test: A Valuable Tool to Assess Tactile Hypersensitivity in Mice
    胡须损伤测试:一种检测小鼠触觉过敏症的有效方法
    作者:Luigi Balasco, Gabriele Chelini, Yuri Bozzi and Giovanni Provenzano日期:08/20/2019,浏览量:381,Q&A: 0
    Abnormal response to tactile stimulation, described as both hyper- and hypo-reactivity, is a common sensory impairment in multiple neuropsychiatric disorders. The neural bases of tactile sensitivity remain so far unknown. In the last years, animal ...
    Genotyping of the OATP1B1 c. 521 T>C Polymorphism from the Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Specimens: An Optimized Protocol
    对FFPE组织样品中OATP1B1 c. 521 T>C多态性进行基因分型的优化实验方法
    作者:Alexandra R Crowe, Jonathan Miller and Wei Yue日期:08/20/2019,浏览量:240,Q&A: 0
    Organic anion transporting polypeptide (OATP) 1B1 is a liver-specific transport protein that plays an important role in hepatic drug disposition. It transports many drugs from the blood into the liver, including lipid-lowering statins. The c. 521 ...
    Straight Channel Microfluidic Chips for the Study of Platelet Adhesion under Flow
    直通道微流控芯片用于研究流动中的血小板黏附
    作者:Alexander Dupuy, Lining Arnold Ju and Freda H Passam日期:03/20/2019,浏览量:2261,Q&A: 0
    Microfluidic devices have become an integral method of cardiovascular research as they enable the study of shear force in biological processes, such as platelet function and thrombus formation. Furthermore, microfluidic chips offer the benefits of ...
    Primary Embryonic Rat Cortical Neuronal Culture and Chronic Rotenone Treatment in Microfluidic Culture Devices
    原代胚胎大鼠皮质神经元培养并长期在微流控培养装置中鱼藤酮处理
    作者:Victor S. Van Laar, Beth Arnold and Sarah B Berman日期:03/20/2019,浏览量:1270,Q&A: 0
    In the study of neurodegenerative diseases, it is imperative to study the cellular and molecular changes associated with pathogenesis in the relevant cell type, central nervous system neurons. The unique compartmentalized morphology and bioenergetic ...
    Quantification of Queuosine Modification Levels in tRNA from Human Cells Using APB Gel and Northern Blot
    利用聚乙二醇凝胶和Northern印记法定量分析来自人细胞的tRNA 的Q核苷修饰水平
    作者:Zaneta Matuszek and Tao Pan日期:03/20/2019,浏览量:1097,Q&A: 0
    Queuosine (Q) is a hypermodified base in the wobble anticodon position of tRNAs coding for the amino acids Tyr, His, Asn, and Asp. tRNA Q-modification is introduced by a queuine tRNA-ribosyltransferase (TGT) that replaces the guanine base at G34 at ...
    Social Defeat Stress (SDS) in Mice: Using Swiss Mice as Resident
    Swiss小鼠作为研究对象研究小鼠的社会失败应激(SDS)
    作者:Marco Oreste F. Pagliusi Jr. and Cesar R. Sartori日期:03/20/2019,浏览量:1334,Q&A: 0
    Due to the high prevalence and great economic impact of depression, studies with animal models have been increasingly used to identify neurobiological mechanisms associated with this disorder. However, many animal models use stressful conditions ...
    Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes
    大鼠肾脏单纯线粒体的分离和线粒体呼吸链复合物蛋白质免疫印迹
    [Abstract] Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell cycle and metabolism. Thus, mitochondrial dysfunction is a hallmark of many chronic diseases ...
    Isolation and Transcriptomic Profiling of Single Myofibers from Mice
    小鼠单根肌纤维的分离和转录组分析
    [Abstract] Skeletal muscle is composed of different cells and myofiber types, with distinct metabolic and structural features. Generally, transcriptomic analysis of skeletal muscle is performed using whole muscle, resulting in average information as all cells composing the organ contribute to the expression value detected for each gene with the loss of ...
    Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices
    反义寡脱氧核苷酸灌注可阻突触可塑性相关蛋白在海马切片上的基因表达且无诱导补偿作用
    [Abstract] The elucidation of the molecular mechanisms of long-term synaptic plasticity has been hindered by both the compensation that can occur after chronic loss of the core plasticity molecules and by ex vivo conditions that may not reproduce in vivo plasticity. Here we describe a novel method to rapidly suppress gene expression by ...
    ATAC-seq on Sorted Adult Mouse Neurons
    ATAC-seq用于分选成熟小鼠神经元
    作者:Erin A. Clark, Yasuyuki Shima and Sacha Nelson日期:10/05/2019,浏览量:186,Q&A: 0
    [Abstract] Transcription regulation is a key aspect of cellular identity established during development and maintained into adulthood. Molecular and biochemical assays that probe the genome are critical tools in exploring mechanisms of transcription regulation and cell type identity. The mammalian brain is composed of a huge diversity of cell types with ...
    3D Organoid Formation from the Murine Salivary Gland Cell Line SIMS
    小鼠唾液腺SIMS细胞系的三维类器官形成
    作者:Harleen K. Athwal and Isabelle M. A. Lombaert日期:10/05/2019,浏览量:148,Q&A: 0
    [Abstract] Salivary glands consist of multiple phenotypically and functionally unique cell populations, such as the acinar, ductal, and myoepithelial cells that help produce, modify, and secrete saliva (Lombaert et al., 2011). Identification of mechanisms and factors that regulate these populations has been of key interest, as salivary gland-related ...
    An Operant Conditioning Task to Assess the Choice between Wheel Running and Palatable Food in Mice
    一项小鼠在转轮装置和可口食物之间选择的操作式条件反射测试
    作者:Bastien Redon, Imane Hurel, Giovanni Marsicano and Francis Chaouloff日期:10/05/2019,浏览量:219,Q&A: 0
    [Abstract] Wheel running, especially in the homecage, has been widely used to study the neurobiology of exercise because animal tends to use it voluntarily. However, as for each reward, its consumption (in the present case, running performance) does not specifically provide information on its incentive value, i.e., the extent to which animals are ...
    Reconstituting Breast Tissue with Organotypic Three-dimensional Co-culture of Epithelial and Stromal Cells in Discontinuous Extracellular Matrices
    通过不连续细胞外基质中上皮细胞和基质细胞三维共培养重建乳腺组织
    作者:Gang Ren, Vandana Sharma, Joshua Letson, Yashna Walia, Veani Fernando and Saori Furuta日期:10/05/2019,浏览量:209,Q&A: 0
    [Abstract] Co-culture systems utilizing reconstituted or synthetic extracellular matrix (ECM) and micropatterning techniques have enabled the reconstruction of surface epithelial tissues. This technique has been utilized in the regeneration, disease modeling and drug screening of the surface epithelia, such as the skin and esophagus. On the other hand, the ...
    Imaging the Vasculature of Immunodeficient Mice Using Positron Emission Tomography/Computed Tomography (PET/CT) and 18F-fluorodeoxyglucose Labeled Human Erythrocytes
    利用正电子发射断层扫描/计算机断层扫描(PET、CT)和18F-氟脱氧葡萄糖标记人类红细胞 进行免疫缺陷小鼠脉管系统成像
    作者:Shaowei Wang and Jung W. Choi日期:10/05/2019,浏览量:109,Q&A: 0
    [Abstract] Nuclear blood pool imaging using radiolabeled red blood cells has been used in the clinical setting for the evaluation of a number of medical conditions including gastrointestinal hemorrhage, impaired cardiac contractility, and altered cerebrovascular blood flow. Nuclear blood pool imaging is typically performed using Technetium-99m-labeled (99m ...
    A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-based Scaffolds (3D-dCBS)
    一种获得去细胞牛脊髓细胞外基质构成支架的新方法
    作者:Yavuz E. Arslan, Burcu Efe and Tugba Sezgin Arslan日期:10/05/2019,浏览量:145,Q&A: 0
    [Abstract] Extracellular matrix (ECM)-based tissue engineering scaffolds have an essential role in promoting tissue regeneration. Nerve tissue engineering aims at facilitating the repair of permanent damage to the peripheral and central nervous systems, which are difficult to heal. For this purpose, a variety of biomaterials are being developed consisting of ...
    Simple Protocol for Distinguishing Drug-induced Effects on Spatial Memory Acquisition, Consolidation and Retrieval in Mice Using the Morris Water Maze
    一种利用Morris水迷宫分辨药物诱导对于小鼠空间记忆获取、巩固和恢复学习行为影响的简单方案
    作者:Ariel K. Frame, Asad Lone, Richard A. Harris and Robert C. Cumming日期:09/20/2019,浏览量:206,Q&A: 0
    [Abstract] The Morris water maze (MWM) is one of the most commonly used tests for assessing spatial learning and memory in mice. While the MWM is highly amenable to testing the effects of memory modifying drugs, most studies do not consider the timing or duration of drug exposure when conducting the MWM assay; factors that can strongly influence the effect ...