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A peer-reviewed protocol journal. No publication fee; free to access
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系统生物学
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Subcellular Fractionation of Hela Cells for Lysosome Enrichment Using a Continuous Percoll-density Gradient
作者地图
分类
现刊
日期
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Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs
通过电镜样品制备实现的人体组织和器官中细胞连接体及其定位的纳米与介观图谱分析
作者：
Lucy Ngo
,
Anton D. Nathanson
,
Tomasz Garbowski
,
Ulf Knothe
,
Dirk Zeidler
and
Melissa L. Knothe Tate
，
日期：
07/20/2019，
浏览量：
727，
Q&A：
0
[Abstract] Multibeam scanning electron microscopy (multiSEM) provides a technical platform for seamless nano-to-mesoscale mapping of cells in human tissues and organs, which is a major new initiative of the U.S. National Institutes of Health. Such cross-length-scale imaging is expected to provide unprecedented understanding of relationships between cellular ...
A Protocol to Map the Spatial Proteome Using HyperLOPIT in
Saccharomyces cerevisiae
利用HyperLOPIT绘制酿酒酵母空间蛋白质组图谱的方法
作者：
Daniel J. H. Nightingale
,
Kathryn S. Lilley
and
Stephen G. Oliver
，
日期：
07/20/2019，
浏览量：
787，
Q&A：
0
[Abstract] The correct subcellular localization of proteins is vital for cellular function and the study of this process at the systems level will therefore enrich our understanding of the roles of proteins within the cell. Multiple methods are available for the study of protein subcellular localization, including fluorescence microscopy, organelle ...
Genome-wide Estimation of Evolutionary Distance and Phylogenetic Analysis of Homologous Genes
进化距离的全基因组评估和同源基因的系统进化分析
作者：
Meixia Zhao
,
Biao Zhang
,
Jianxin Ma
and
Damon Lisch
，
日期：
12/05/2018，
浏览量：
1951，
Q&A：
0
[Abstract] Homologous genes, including paralogs and orthologs, are genes that share sequence homologies within or between different species. Homologous genes originate from a common origin through speciation, genetic duplication or horizontal gene transfer. Estimation of the sequence divergence of homologous genes help us to understand divergence time, which ...
Use of Gas Chromatography to Quantify Short Chain Fatty Acids in the Serum, Colonic Luminal Content and Feces of Mice
利用气相色谱法定量分析小鼠血清、结肠管腔内容物和粪便中短链脂肪酸
作者：
Willian Rodrigues Ribeiro
,
Marco Aurélio Ramirez Vinolo
,
Leandro Augusto Calixto
and
Caroline Marcantonio Ferreira
，
日期：
11/20/2018，
浏览量：
3962，
Q&A：
0
[Abstract] Short-Chain Fatty Acids (SCFAs) are a product of the fermentation of resistant starches and dietary fibers by the gut microbiota. The most important SCFA are acetate (C2), propionate (C3) and butyrate (C4). These metabolites are formed and absorbed in the colon and then transported through the hepatic vein to the liver. SCFAs are more concentrated ...
Stable-isotope Labeled Metabolic Analysis in
Drosophila melanogaster
: From Experimental Setup to Data Analysis
黑腹果蝇的稳定同位素标记代谢分析：从实验建立到数据分析
作者：
Yuping Cai
,
Nan Liu
and
Zheng-Jiang Zhu
，
日期：
09/20/2018，
浏览量：
2021，
Q&A：
0
[Abstract] Stable-isotope labeled metabolic analysis is an essential methodology to characterize metabolic regulation during biological processes. However, the method using stable-isotope-labeled tracer (
e.g.
,
13
C-glucose) in live animal is only beginning to be developed. Here, we contribute a qualitative metabolic labeling experiment ...
Quantitative ChIP-seq by Adding Spike-in from Another Species
通过引入另一物种的标准参照以定量分析ChIP-seq
作者：
Kongyan Niu
,
Rui Liu
and
Nan Liu
，
日期：
08/20/2018，
浏览量：
3828，
Q&A：
0
[Abstract] Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a routine procedure in the lab; however, epigenome-wide quantitative comparison among independent ChIP-seq experiments remains a challenge. Here, we contribute an experimental protocol combined with a computational workflow allowing quantitative and comparative assessment of ...
Tethered Chromosome Conformation Capture Sequencing in Triticeae: A Valuable Tool for Genome Assembly
小麦族系中染色体构象捕获测序：一个有价值的基因组组装工具
作者：
Axel Himmelbach
,
Ines Walde
,
Martin Mascher
and
Nils Stein
，
日期：
08/05/2018，
浏览量：
2698，
Q&A：
0
[Abstract] Chromosome conformation capture sequencing (Hi-C) is a powerful method to comprehensively interrogate the three-dimensional positioning of chromatin in the nucleus. The development of Hi-C can be traced back to successive increases in the resolution and throughput of chromosome conformation capture (3C) (Dekker
et al.
, 2002). The basic ...
Bacterial Microcolonies in Gel Beads for High-throughput Screening
凝胶珠细菌微菌落的高通量筛选
作者：
Yolanda Schaerli
，
日期：
07/05/2018，
浏览量：
3394，
Q&A：
0
[Abstract] High-throughput screening of a DNA library expressed in a bacterial population for identifying potentially rare members displaying a property of interest is a crucial step for success in many experiments such as directed evolution of proteins and synthetic circuits and deep mutational scanning to identify gain- or loss-of-function mutants.
...
Extraction and 16S rRNA Sequence Analysis of Microbiomes Associated with Rice Roots
水稻根系相关微生物群落的分离及其16S rRNA序列分析
作者：
Joseph Edwards
,
Christian Santos-Medellín
and
Venkatesan Sundaresan
，
日期：
06/20/2018，
浏览量：
5557，
Q&A：
0
[Abstract] Plant roots associate with a wide diversity of bacteria and archaea across the root-soil spectrum. The rhizosphere microbiota, the communities of microbes in the soil adjacent to the root, can contain up to 10 billion bacterial cells per gram of soil (Raynaud and Nunan, 2014) and can play important roles for the fitness of the host plant. Subsets ...
Transient Expression Assay in
NahG
Arabidopsis Plants Using
Agrobacterium tumefaciens
使用根癌农杆菌在
NahG
拟南芥植株中进行瞬时表达分析
作者：
Pepe Cana-Quijada
,
Eduardo R. Bejarano
,
Rosa Lozano-Durán
and
Tábata Rosas-Díaz
，
日期：
06/20/2018，
浏览量：
3386，
Q&A：
1
[Abstract]
Agrobacterium
-mediated transient expression has greatly contributed to research in molecular plant biology but has low efficiency and inconsistency in
Arabidopsis thaliana
(
Arabidopsis
). Here, we describe a simple, efficient and fast protocol to make transient gene expression in
NahG
Arabidopsis plants using
...