系统生物学

Delayed Alternation Task for the Study of Spatial Working and Long Term Memory in Rats

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现刊

Experiments for in silico evaluation of Optimality of Photosynthetic Nitrogen Distribution and Partitioning in the Canopy: an Example Using Greenhouse Cucumber Plants
树冠中光合氮分配的最适生物信息学评估实验：温室黄瓜应用实例

作者：Yi-Chen Pao, Tsu-Wei Chen, Dany Pascal Moualeu-Ngangue and Hartmut Stützel，日期：03/20/2020，浏览量：274，Q&A： 0

Acclimation of leaf traits to fluctuating environments is a key mechanism to maximize fitness. One of the most important strategies in acclimation to changing light is to maintain efficient utilization of nitrogen in the photosynthetic apparatus by ...

Delayed Alternation Task for the Study of Spatial Working and Long Term Memory in Rats
延迟交替任务用于研究大鼠空间工作记忆和长期记忆

作者：Megi Hoxha and Marta Sabariego，日期：03/05/2020，浏览量：414，Q&A： 0

Memory systems can hold previously presented information for several seconds, bridging gaps between discontinuous events. It has been previously demonstrated that the hippocampus and the medial entorhinal cortex (mEC) are necessary for memory ...

mRNA Extraction from Gill Tissue for RNA-sequencing
鳃组织mRNA 提取用于RNA测序

作者：Jukka-Pekka Verta and Felicity Jones，日期：03/05/2020，浏览量：308，Q&A： 0

Adaptation is thought to proceed in part through spatial and temporal changes in gene expression. Fish species such as the threespine stickleback are powerful vertebrate models to study the genetic architecture of adaptive changes in gene expression ...

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作者：Yi-Chen Pao, Tsu-Wei Chen, Dany Pascal Moualeu-Ngangue and Hartmut Stützel，日期：03/20/2020，浏览量：274，Q&A： 0

[Abstract] Acclimation of leaf traits to fluctuating environments is a key mechanism to maximize fitness. One of the most important strategies in acclimation to changing light is to maintain efficient utilization of nitrogen in the photosynthetic apparatus by continuous modifications of between-leaf distribution along the canopy depth and within-leaf ...

Delayed Alternation Task for the Study of Spatial Working and Long Term Memory in Rats
延迟交替任务用于研究大鼠空间工作记忆和长期记忆

作者：Megi Hoxha and Marta Sabariego，日期：03/05/2020，浏览量：414，Q&A： 0

[Abstract] Memory systems can hold previously presented information for several seconds, bridging gaps between discontinuous events. It has been previously demonstrated that the hippocampus and the medial entorhinal cortex (mEC) are necessary for memory retention over delay intervals in alternation tasks. Here we describe the delayed alternation task, a ...

mRNA Extraction from Gill Tissue for RNA-sequencing
鳃组织mRNA 提取用于RNA测序

作者：Jukka-Pekka Verta and Felicity Jones，日期：03/05/2020，浏览量：308，Q&A： 0

[Abstract] Adaptation is thought to proceed in part through spatial and temporal changes in gene expression. Fish species such as the threespine stickleback are powerful vertebrate models to study the genetic architecture of adaptive changes in gene expression since divergent adaptation to different environments is common, they are abundant and easy to study ...

ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列

作者：Junaid Akhtar, Piyush More and Steffen Albrecht，日期：02/20/2020，浏览量：267，Q&A： 0

[Abstract] Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as ...

Mapping the Mechanome–A Protocol for Simultaneous Live Imaging and Quantitative Analysis of Cell Mechanoadaptation and Ingression
力学组学映射—一种用于同时进行实时成像和细胞力学适应和侵入定量分析的方案

作者：Vina D. L. Putra, Iman Jalilian, Madeline Campbell, Kate Poole, Renee Whan, Florence Tomasetig and Melissa L. Knothe Tate，日期：12/05/2019，浏览量：727，Q&A： 0

[Abstract] Mechanomics, the mechanics equivalent of genomics, is a burgeoning field studying mechanical modulation of stem cell behavior and lineage commitment. Analogous to mechanical testing of a living material as it adapts and evolves, mapping of the mechanome necessitates the development of new protocols to assess changes in structure and function in ...

Isolation and Quantification of Metabolite Levels in Murine Tumor Interstitial Fluid by LC/MS
LC/MS法对小鼠肿瘤间质液中代谢产物的分离和定量

作者：Mark R Sullivan, Caroline A Lewis and Alexander Muir，日期：11/20/2019，浏览量：1488，Q&A： 0

[Abstract] Cancer is a disease characterized by altered metabolism, and there has been renewed interest in understanding the metabolism of tumors. Even though nutrient availability is a critical determinant of tumor metabolism, there has been little systematic study of the nutrients directly available to cancer cells in the tumor microenvironment. Previous ...

Probabilistic Models for Predicting Mutational Routes to New Adaptive Phenotypes
用于预测新的适应性表型突变路径的概率模型

作者：Eric Libby and Peter A. Lind，日期：10/20/2019，浏览量：901，Q&A： 0

[Abstract] Understanding the translation of genetic variation to phenotypic variation is a fundamental problem in genetics and evolutionary biology. The introduction of new genetic variation through mutation can lead to new adaptive phenotypes, but the complexity of the genotype-to-phenotype map makes it challenging to predict the phenotypic effects of ...

Non-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments of Arabidopsis Leaf Tissues
非水体系分离（NAF）用于拟南芥叶组织亚细胞组分的代谢研究

作者：David B. Medeiros, Stéphanie Arrivault, Jessica Alpers, Alisdair R. Fernie and Fayezeh Arabi，日期：10/20/2019，浏览量：721，Q&A： 0

[Abstract] The accurate determination of metabolite distribution in subcellular compartments is still challenging in plant science. Various methodologies, such as fluorescence resonance energy transfer-based technology, nuclear magnetic resonance spectroscopy and protoplast fractionation allow the study of metabolite compartmentation. However, large changes ...

Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs
通过电镜样品制备实现的人体组织和器官中细胞连接体及其定位的纳米与介观图谱分析

作者：Lucy Ngo, Anton D. Nathanson, Tomasz Garbowski, Ulf Knothe, Dirk Zeidler and Melissa L. Knothe Tate，日期：07/20/2019，浏览量：1733，Q&A： 0

[Abstract] Multibeam scanning electron microscopy (multiSEM) provides a technical platform for seamless nano-to-mesoscale mapping of cells in human tissues and organs, which is a major new initiative of the U.S. National Institutes of Health. Such cross-length-scale imaging is expected to provide unprecedented understanding of relationships between cellular ...

A Protocol to Map the Spatial Proteome Using HyperLOPIT in Saccharomyces cerevisiae
利用HyperLOPIT绘制酿酒酵母空间蛋白质组图谱的方法

作者：Daniel J. H. Nightingale, Kathryn S. Lilley and Stephen G. Oliver，日期：07/20/2019，浏览量：1415，Q&A： 0

[Abstract] The correct subcellular localization of proteins is vital for cellular function and the study of this process at the systems level will therefore enrich our understanding of the roles of proteins within the cell. Multiple methods are available for the study of protein subcellular localization, including fluorescence microscopy, organelle ...