系统生物学

Surface Plasmon Resonance Analysis of the Protein-protein Binding Specificity Using Autolab ESPIRIT

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现刊

ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列

作者：Junaid Akhtar, Piyush More and Steffen Albrecht，日期：02/20/2020，浏览量：121，Q&A： 0

Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. ...

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作者：Junaid Akhtar, Piyush More and Steffen Albrecht，日期：02/20/2020，浏览量：121，Q&A： 0

[Abstract] Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as ...

Mapping the Mechanome–A Protocol for Simultaneous Live Imaging and Quantitative Analysis of Cell Mechanoadaptation and Ingression
力学组学映射—一种用于同时进行实时成像和细胞力学适应和侵入定量分析的方案

作者：Vina D. L. Putra, Iman Jalilian, Madeline Campbell, Kate Poole, Renee Whan, Florence Tomasetig and Melissa L. Knothe Tate，日期：12/05/2019，浏览量：603，Q&A： 0

[Abstract] Mechanomics, the mechanics equivalent of genomics, is a burgeoning field studying mechanical modulation of stem cell behavior and lineage commitment. Analogous to mechanical testing of a living material as it adapts and evolves, mapping of the mechanome necessitates the development of new protocols to assess changes in structure and function in ...

Isolation and Quantification of Metabolite Levels in Murine Tumor Interstitial Fluid by LC/MS
LC/MS法对小鼠肿瘤间质液中代谢产物的分离和定量

作者：Mark R Sullivan, Caroline A Lewis and Alexander Muir，日期：11/20/2019，浏览量：1285，Q&A： 0

[Abstract] Cancer is a disease characterized by altered metabolism, and there has been renewed interest in understanding the metabolism of tumors. Even though nutrient availability is a critical determinant of tumor metabolism, there has been little systematic study of the nutrients directly available to cancer cells in the tumor microenvironment. Previous ...

Probabilistic Models for Predicting Mutational Routes to New Adaptive Phenotypes
用于预测新的适应性表型突变路径的概率模型

作者：Eric Libby and Peter A. Lind，日期：10/20/2019，浏览量：806，Q&A： 0

[Abstract] Understanding the translation of genetic variation to phenotypic variation is a fundamental problem in genetics and evolutionary biology. The introduction of new genetic variation through mutation can lead to new adaptive phenotypes, but the complexity of the genotype-to-phenotype map makes it challenging to predict the phenotypic effects of ...

Non-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments of Arabidopsis Leaf Tissues
非水体系分离（NAF）用于拟南芥叶组织亚细胞组分的代谢研究

作者：David B. Medeiros, Stéphanie Arrivault, Jessica Alpers, Alisdair R. Fernie and Fayezeh Arabi，日期：10/20/2019，浏览量：624，Q&A： 0

[Abstract] The accurate determination of metabolite distribution in subcellular compartments is still challenging in plant science. Various methodologies, such as fluorescence resonance energy transfer-based technology, nuclear magnetic resonance spectroscopy and protoplast fractionation allow the study of metabolite compartmentation. However, large changes ...

Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs
通过电镜样品制备实现的人体组织和器官中细胞连接体及其定位的纳米与介观图谱分析

作者：Lucy Ngo, Anton D. Nathanson, Tomasz Garbowski, Ulf Knothe, Dirk Zeidler and Melissa L. Knothe Tate，日期：07/20/2019，浏览量：1539，Q&A： 0

[Abstract] Multibeam scanning electron microscopy (multiSEM) provides a technical platform for seamless nano-to-mesoscale mapping of cells in human tissues and organs, which is a major new initiative of the U.S. National Institutes of Health. Such cross-length-scale imaging is expected to provide unprecedented understanding of relationships between cellular ...

A Protocol to Map the Spatial Proteome Using HyperLOPIT in Saccharomyces cerevisiae
利用HyperLOPIT绘制酿酒酵母空间蛋白质组图谱的方法

作者：Daniel J. H. Nightingale, Kathryn S. Lilley and Stephen G. Oliver，日期：07/20/2019，浏览量：1323，Q&A： 0

[Abstract] The correct subcellular localization of proteins is vital for cellular function and the study of this process at the systems level will therefore enrich our understanding of the roles of proteins within the cell. Multiple methods are available for the study of protein subcellular localization, including fluorescence microscopy, organelle ...

Genome-wide Estimation of Evolutionary Distance and Phylogenetic Analysis of Homologous Genes
进化距离的全基因组评估和同源基因的系统进化分析

作者：Meixia Zhao, Biao Zhang, Jianxin Ma and Damon Lisch，日期：12/05/2018，浏览量：2638，Q&A： 0

[Abstract] Homologous genes, including paralogs and orthologs, are genes that share sequence homologies within or between different species. Homologous genes originate from a common origin through speciation, genetic duplication or horizontal gene transfer. Estimation of the sequence divergence of homologous genes help us to understand divergence time, which ...

Use of Gas Chromatography to Quantify Short Chain Fatty Acids in the Serum, Colonic Luminal Content and Feces of Mice
利用气相色谱法定量分析小鼠血清、结肠管腔内容物和粪便中短链脂肪酸

作者：Willian Rodrigues Ribeiro, Marco Aurélio Ramirez Vinolo, Leandro Augusto Calixto and Caroline Marcantonio Ferreira，日期：11/20/2018，浏览量：5518，Q&A： 0

[Abstract] Short-Chain Fatty Acids (SCFAs) are a product of the fermentation of resistant starches and dietary fibers by the gut microbiota. The most important SCFA are acetate (C2), propionate (C3) and butyrate (C4). These metabolites are formed and absorbed in the colon and then transported through the hepatic vein to the liver. SCFAs are more concentrated ...

Stable-isotope Labeled Metabolic Analysis in Drosophila melanogaster: From Experimental Setup to Data Analysis
黑腹果蝇的稳定同位素标记代谢分析：从实验建立到数据分析

作者：Yuping Cai, Nan Liu and Zheng-Jiang Zhu，日期：09/20/2018，浏览量：2654，Q&A： 0

[Abstract] Stable-isotope labeled metabolic analysis is an essential methodology to characterize metabolic regulation during biological processes. However, the method using stable-isotope-labeled tracer (e.g., ¹³C-glucose) in live animal is only beginning to be developed. Here, we contribute a qualitative metabolic labeling experiment ...