癌症生物学

分类

    现刊
    Single-cell qPCR Assay with Massively Parallel Microfluidic System
    大规模平行微流控系统用于单细胞qPCR检测
    作者:Marta Prieto-Vila, Takahiro Ochiya and Yusuke Yamamoto日期:03/20/2020,浏览量:210,Q&A: 0
    The single-cell transcriptome is the set of messenger RNA molecules expressed in one cell. It is extremely variable and changes according to external, physical and biochemical conditions. Due to sensitivity shortages, most of genetic studies use ...
    Single-cell qPCR Assay with Massively Parallel Microfluidic System
    大规模平行微流控系统用于单细胞qPCR检测
    作者:Marta Prieto-Vila, Takahiro Ochiya and Yusuke Yamamoto日期:03/20/2020,浏览量:210,Q&A: 0
    [Abstract] The single-cell transcriptome is the set of messenger RNA molecules expressed in one cell. It is extremely variable and changes according to external, physical and biochemical conditions. Due to sensitivity shortages, most of genetic studies use bulk samples, providing only the average gene expression. Single-cell technologies have provided a ...
    Rapid Nickel-based Isolation of Extracellular Vesicles from Different Biological Fluids
    从不同的生物体液中进行胞外囊泡的快速镍基分离
    [Abstract] Extracellular vesicles (EVs) are membranous structures that cells massively release in extracellular fluids. EVs are cargo of cellular components such as lipids, proteins, and nucleic acids that can work as a formidable source in liquid biopsy studies searching for disease biomarkers. We recently demonstrated that nickel-based isolation (NBI) is a ...
    Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH
    利用显色原位杂交进行固定细胞和组织中单个RNA的检测
    作者:Meng Jiang, Chen Lin and Rongqin Ke日期:02/05/2020,浏览量:333,Q&A: 0
    [Abstract] Visualization of RNA molecules in situ helps to better understand the functions of expressed genes. Currently, most conventional in situ hybridization methods for visualization of individual RNAs are based on fluorescence detection. Herein we present a chromogenic in situ hybridization protocol for visualization of ...
    Identification of Target Protein for Bio-active Small Molecule Using Photo-cross Linked Beads and MALDI-TOF Mass Spectrometry
    光交联微球和MALDI-TOF质谱法鉴定生物活性小分子靶蛋白
    作者:Kruthi Suvarna, Kaori Honda, Makoto Muroi, Yasumitsu Kondoh, Nobumoto Watanabe and Hiroyuki Osada日期:02/05/2020,浏览量:363,Q&A: 0
    [Abstract] Development of methods for protein identification is one of the important aspects of proteomics. Here, we report a protocol for the preparation of compound conjugated beads by photo-crosslinking, affinity purification, gel electrophoresis, and highly sensitive mass spectrometric assay for drug-target identification. Although there are several ...
    Measurement of ATPase Activity of Valosin-containing Protein/p97
    含缬酪肽蛋白/p97的ATP酶活性检测
    作者:Kruthi Suvarna, Kaori Honda, Makoto Muroi, Yasumitsu Kondoh, Hiroyuki Osada and Nobumoto Watanabe日期:02/05/2020,浏览量:393,Q&A: 0
    [Abstract] Valosin-containing protein (VCP; also known as p97) is a type II ATPase regulating several cellular processes. Using proteomic techniques, we identified a chemical compound that binds to the D1 ATPase domain of VCP. The protocol described here was to study the effect of the compound on ATPase activity in vitro of purified VCP protein. ...
    Approaching RNA-seq for Cell Line Identification
    RNA序列在细胞系鉴定中的应用
    作者:Tabrez A. Mohammad and Yidong Chen日期:02/05/2020,浏览量:516,Q&A: 0
    [Abstract] Cancer cell lines serve as invaluable model systems for cancer biology research and help in evaluating the efficacy of new therapeutic agents. However, cell line contamination and misidentification have become one of the most pressing problems affecting biomedical research. Available methods of cell line authentication suffer from limited access, ...
    Bone-in-culture Array to Model Bone Metastasis in ex vivo Condition
    体外骨碎片培养列阵模拟骨转移
    作者:Hai Wang and Xiang H.-F. Zhang日期:01/20/2020,浏览量:481,Q&A: 0
    [Abstract] Bone is the most frequently affected organ by metastases of breast cancer and prostate cancer. Our knowledge on bone metastasis is extremely limited due to the lack of potent and efficient experimental models. We developed the “Bone-In-Culture Array (BICA)” platform to model the bone colonization of cancer cells in ex vivo cultures. The ...
    Automated Analysis of Cell Surface Ruffling: Ruffle Quantification Macro
    细胞表面Ruffling的自动分析:Ruffle的宏观量化
    作者:Nicholas D. Condon, Jennifer L. Stow and Adam A. Wall日期:01/20/2020,浏览量:399,Q&A: 0
    [Abstract] Cell surface protrusions include F-actin rich, wave-like ruffles that are erected transiently in response to stimuli and during cell migration. Macrophages are innate immune cells that ruffle constitutively and more dramatically in cells activated by pathogens. Dorsal ruffles and their resulting macropinosomes are key sites for environmental ...
    Isolation of Stem Cells, Endothelial Cells and Pericytes from Human Infantile Hemangioma
    人婴幼儿血管瘤中干细胞、内皮细胞和周细胞的分离
    作者:Lan Huang and Joyce Bischoff日期:01/20/2020,浏览量:418,Q&A: 0
    [Abstract] Infantile hemangioma (IH) is a vascular tumor noted for its excessive blood vessel formation during infancy, glucose-transporter-1 (GLUT1)-positive staining of the blood vessels, and its slow spontaneous involution over several years in early childhood. For most children, IH poses no serious threat because it will eventually involute, but a subset ...
    Analysis of Random Migration of Cancer Cells in 3D
    癌细胞随机迁移的3D分析
    作者:Sai P. Visweshwaran and Alexis Gautreau日期:01/05/2020,浏览量:641,Q&A: 0
    [Abstract] The ability of cancer cells to migrate through a complex three-dimensional (3D) environment is a hallmark event of cancer metastasis. Therefore, an in vitro migration assay to evaluate cancer cell migration in a 3D setting is valuable to examine cancer progression. Here, we describe such a simple migration assay in a 3D ...