癌症生物学

分类

    现刊
    Imaging the Vasculature of Immunodeficient Mice Using Positron Emission Tomography/Computed Tomography (PET/CT) and 18F-fluorodeoxyglucose Labeled Human Erythrocytes
    利用正电子发射断层扫描/计算机断层扫描(PET、CT)和18F-氟脱氧葡萄糖标记人类红细胞 进行免疫缺陷小鼠脉管系统成像
    作者:Shaowei Wang and Jung W. Choi日期:10/05/2019,浏览量:109,Q&A: 0
    Nuclear blood pool imaging using radiolabeled red blood cells has been used in the clinical setting for the evaluation of a number of medical conditions including gastrointestinal hemorrhage, impaired cardiac contractility, and altered ...
    Conjugation of Fab’ Fragments with Fluorescent Dyes for Single-molecule Tracking on Live Cells
    Fab片段结合荧光染料用于活细胞单分子示踪
    作者:I-Ting Teng, Xiangning Bu and Inhee Chung日期:09/20/2019,浏览量:241,Q&A: 0
    Our understanding of the regulation and functions of cell-surface proteins has progressed rapidly with the advent of advanced optical imaging techniques. In particular, single-molecule tracking (SMT) using bright fluorophores conjugated to ...
    SIRF: A Single-cell Assay for in situ Protein Interaction with Nascent DNA Replication Forks
    SIRF:一种蛋白与新生DNA复制叉原位相互作用的单细胞检测办法
    作者:Sunetra Roy and Katharina Schlacher日期:09/20/2019,浏览量:300,Q&A: 0
    The duplication of DNA is a fundamental process that is required for the transfer of the genetic information from parent to daughter cells. Aberrant DNA replication processes are associated with diverse disease phenotypes, including developmental ...
    A Cell Culture Model that Mimics Physiological Tissue Oxygenation Using Oxygen-permeable Membranes
    一种利用透氧膜模拟生理组织氧合的细胞培养模式
    作者:Chloe-Anne Martinez, Peter A. Cistulli and Kristina M. Cook日期:09/20/2019,浏览量:293,Q&A: 0
    Dissolved oxygen and its availability to cells in culture is an overlooked variable which can have significant consequences on experimental research outcomes, including reproducibility. Oxygen sensing pathways play key roles in cell growth and ...
    A Robust, One-step FRET Assay for Human Heparanase
    人乙酰肝素酶的一步快速FRET检测
    作者:Jyothi C. Sistla and Umesh R. Desai日期:09/05/2019,浏览量:296,Q&A: 0
    Heparanase, an endo-β-D-glucuronidase, cleaves cell surface and extracellular matrix heparan sulfate (HS) chains at distinct sites and plays important biological roles including modulation of cell growth and metastasis. Although a number of ...
    Three-dimensional Reconstruction and Quantification of Proteins and mRNAs at the Single-cell Level in Cultured Cells
    培养细胞中蛋白和mRNA在单细胞水平上的三维重构和量化分析
    作者:Xueer Jiang, Ingrid Brust-Mascher and Li-En Jao日期:08/20/2019,浏览量:649,Q&A: 0
    Gene expression is often regulated by the abundance, localization, and translation of mRNAs in both space and time. Being able to visualize mRNAs and protein products in single cells is critical to understand this regulatory process. The development ...
    Imaging the Vasculature of Immunodeficient Mice Using Positron Emission Tomography/Computed Tomography (PET/CT) and 18F-fluorodeoxyglucose Labeled Human Erythrocytes
    利用正电子发射断层扫描/计算机断层扫描(PET、CT)和18F-氟脱氧葡萄糖标记人类红细胞 进行免疫缺陷小鼠脉管系统成像
    作者:Shaowei Wang and Jung W. Choi日期:10/05/2019,浏览量:109,Q&A: 0
    [Abstract] Nuclear blood pool imaging using radiolabeled red blood cells has been used in the clinical setting for the evaluation of a number of medical conditions including gastrointestinal hemorrhage, impaired cardiac contractility, and altered cerebrovascular blood flow. Nuclear blood pool imaging is typically performed using Technetium-99m-labeled (99m ...
    Conjugation of Fab’ Fragments with Fluorescent Dyes for Single-molecule Tracking on Live Cells
    Fab片段结合荧光染料用于活细胞单分子示踪
    作者:I-Ting Teng, Xiangning Bu and Inhee Chung日期:09/20/2019,浏览量:241,Q&A: 0
    [Abstract] Our understanding of the regulation and functions of cell-surface proteins has progressed rapidly with the advent of advanced optical imaging techniques. In particular, single-molecule tracking (SMT) using bright fluorophores conjugated to antibodies and wide-field microscopy methods such as total internal reflection fluorescence microscopy have ...
    SIRF: A Single-cell Assay for in situ Protein Interaction with Nascent DNA Replication Forks
    SIRF:一种蛋白与新生DNA复制叉原位相互作用的单细胞检测办法
    作者:Sunetra Roy and Katharina Schlacher日期:09/20/2019,浏览量:300,Q&A: 0
    [Abstract] The duplication of DNA is a fundamental process that is required for the transfer of the genetic information from parent to daughter cells. Aberrant DNA replication processes are associated with diverse disease phenotypes, including developmental defects, ageing disorders, blood disorders such as Fanconi Anemia, increased inflammation and cancer. ...
    A Cell Culture Model that Mimics Physiological Tissue Oxygenation Using Oxygen-permeable Membranes
    一种利用透氧膜模拟生理组织氧合的细胞培养模式
    作者:Chloe-Anne Martinez, Peter A. Cistulli and Kristina M. Cook日期:09/20/2019,浏览量:293,Q&A: 0
    [Abstract] Dissolved oxygen and its availability to cells in culture is an overlooked variable which can have significant consequences on experimental research outcomes, including reproducibility. Oxygen sensing pathways play key roles in cell growth and behavior and pericellular oxygen levels should be controlled when establishing in vitro models. ...
    A Robust, One-step FRET Assay for Human Heparanase
    人乙酰肝素酶的一步快速FRET检测
    作者:Jyothi C. Sistla and Umesh R. Desai日期:09/05/2019,浏览量:296,Q&A: 0
    [Abstract] Heparanase, an endo-β-D-glucuronidase, cleaves cell surface and extracellular matrix heparan sulfate (HS) chains at distinct sites and plays important biological roles including modulation of cell growth and metastasis. Although a number of different types of heparanase assays have been reported to date, most are labor intensive, complex ...
    Three-dimensional Reconstruction and Quantification of Proteins and mRNAs at the Single-cell Level in Cultured Cells
    培养细胞中蛋白和mRNA在单细胞水平上的三维重构和量化分析
    作者:Xueer Jiang, Ingrid Brust-Mascher and Li-En Jao日期:08/20/2019,浏览量:649,Q&A: 0
    [Abstract] Gene expression is often regulated by the abundance, localization, and translation of mRNAs in both space and time. Being able to visualize mRNAs and protein products in single cells is critical to understand this regulatory process. The development of single-molecule RNA fluorescence in situ hybridization (smFISH) allows the detection of ...
    Photodynamic Therapy in a 3D Model of Ovarian Cancer
    卵巢癌3D模型中的光动力学治疗
    作者:Shubhankar Nath and Kaitlin Moore日期:08/05/2019,浏览量:460,Q&A: 0
    [Abstract] Photodynamic therapy (PDT), is a clinically-approved light-based anti-cancer treatment modality in which a photoactivatable photosensitizer is irradiated with an appropriate wavelength of light to generate cytotoxic molecules to kill cancer cells. In this article, we describe an in vitro PDT protocol using a 3-dimensional (3D) model of ...
    Evaluation of Genotoxicity by Micronucleus Assay in vitro and by Allium cepa Test in vivo
    利用微核体外分析系统和洋葱体内实验进行遗传毒性测试
    作者:Christina N. Banti and Sotiris K. Hadjikakou日期:07/20/2019,浏览量:936,Q&A: 0
    [Abstract] The in vitro and in vivo genotoxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) is evaluated by the micronucleus test and the Allium cepa assay, respectively. Fetal lung fibroblast cells (MRC-5), normal human corneal epithelial cells (HCEC) and ...
    Total RNA Isolation from Separately Established Monolayer and Hydrogel Cultures of Human Glioblastoma Cell Line
    单层水凝胶培养人胶质母细胞瘤细胞系的全RNA分离
    作者:Manasi P Jogalekar and Elba E Serrano日期:07/20/2019,浏览量:896,Q&A: 0
    [Abstract] Astrocytoma is an invasive carcinoma occurring in the nervous system and currently lacks effective treatment options. A deeper understanding of the mechanisms of tumorigenesis and tumor progression is needed in order to develop novel therapeutic strategies. Recent advances in in vitro culture systems have demonstrated that the use of ...
    The Chick Chorioallantoic Membrane (CAM) Assay as a Three-dimensional Model to Study Autophagy in Cancer Cells
    以鸡胚绒毛尿囊膜(CAM)为三维模型研究癌细胞自噬作用
    作者:Félice A. Janser, Patricia Ney, Marta Teixeira Pinto, Rupert Langer and Mario P. Tschan日期:07/05/2019,浏览量:866,Q&A: 0
    [Abstract] The chick chorioallantoic membrane (CAM) is an extra-embryonic organ and thus well accessible for seeding and harvesting 3D cell cultures. Samples from CAM assays are suitable for protein and gene expression analysis as well as for immuno-histochemical studies. Here we present the CAM assay as a possible model to study autophagy in different types ...