癌症生物学

分类

    现刊
    Three-dimensional Reconstruction and Quantification of Proteins and mRNAs at the Single-cell Level in Cultured Cells
    培养细胞中蛋白和mRNA在单细胞水平上的三维重构和量化分析
    作者:Xueer Jiang, Ingrid Brust-Mascher and Li-En Jao日期:08/20/2019,浏览量:237,Q&A: 0
    Gene expression is often regulated by the abundance, localization, and translation of mRNAs in both space and time. Being able to visualize mRNAs and protein products in single cells is critical to understand this regulatory process. The development ...
    Three-dimensional Reconstruction and Quantification of Proteins and mRNAs at the Single-cell Level in Cultured Cells
    培养细胞中蛋白和mRNA在单细胞水平上的三维重构和量化分析
    作者:Xueer Jiang, Ingrid Brust-Mascher and Li-En Jao日期:08/20/2019,浏览量:237,Q&A: 0
    [Abstract] Gene expression is often regulated by the abundance, localization, and translation of mRNAs in both space and time. Being able to visualize mRNAs and protein products in single cells is critical to understand this regulatory process. The development of single-molecule RNA fluorescence in situ hybridization (smFISH) allows the detection of ...
    Photodynamic Therapy in a 3D Model of Ovarian Cancer
    卵巢癌3D模型中的光动力学治疗
    作者:Shubhankar Nath and Kaitlin Moore日期:08/05/2019,浏览量:250,Q&A: 0
    [Abstract] Photodynamic therapy (PDT), is a clinically-approved light-based anti-cancer treatment modality in which a photoactivatable photosensitizer is irradiated with an appropriate wavelength of light to generate cytotoxic molecules to kill cancer cells. In this article, we describe an in vitro PDT protocol using a 3-dimensional (3D) model of ...
    Evaluation of Genotoxicity by Micronucleus Assay in vitro and by Allium cepa Test in vivo
    利用微核体外分析系统和洋葱体内实验进行遗传毒性测试
    作者:Christina N. Banti and Sotiris K. Hadjikakou日期:07/20/2019,浏览量:587,Q&A: 0
    [Abstract] The in vitro and in vivo genotoxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) is evaluated by the micronucleus test and the Allium cepa assay, respectively. Fetal lung fibroblast cells (MRC-5), normal human corneal epithelial cells (HCEC) and ...
    Total RNA Isolation from Separately Established Monolayer and Hydrogel Cultures of Human Glioblastoma Cell Line
    单层水凝胶培养人胶质母细胞瘤细胞系的全RNA分离
    作者:Manasi P Jogalekar and Elba E Serrano日期:07/20/2019,浏览量:659,Q&A: 0
    [Abstract] Astrocytoma is an invasive carcinoma occurring in the nervous system and currently lacks effective treatment options. A deeper understanding of the mechanisms of tumorigenesis and tumor progression is needed in order to develop novel therapeutic strategies. Recent advances in in vitro culture systems have demonstrated that the use of ...
    The Chick Chorioallantoic Membrane (CAM) Assay as a Three-dimensional Model to Study Autophagy in Cancer Cells
    以鸡胚绒毛尿囊膜(CAM)为三维模型研究癌细胞自噬作用
    作者:Félice A. Janser, Patricia Ney, Marta Teixeira Pinto, Rupert Langer and Mario P. Tschan日期:07/05/2019,浏览量:555,Q&A: 0
    [Abstract] The chick chorioallantoic membrane (CAM) is an extra-embryonic organ and thus well accessible for seeding and harvesting 3D cell cultures. Samples from CAM assays are suitable for protein and gene expression analysis as well as for immuno-histochemical studies. Here we present the CAM assay as a possible model to study autophagy in different types ...
    Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells
    毛细管纳米免疫实验定量分析经CD138筛选的骨髓瘤细胞蛋白
    作者:Irena Misiewicz-Krzeminska, Isabel Isidro and Norma C. Gutiérrez日期:06/20/2019,浏览量:1228,Q&A: 0
    [Abstract] Protein analysis in bone marrow samples from patients with multiple myeloma (MM) has been limited by the low concentration of proteins obtained after CD138+ cell selection. A novel approach based on capillary nano-immunoassay could make it possible to quantify dozens of proteins from each CD138+ purified MM sample in an ...
    Fluorescence HPLC Analysis of the in-vivo Activity of Glucosylceramide Synthase
    葡糖基神经酰胺合酶体内活性的荧光HPLC检测
    作者:Kartik R. Roy, Sachin K. Khiste, Zhijun Liu and Yong-Yu Liu日期:06/20/2019,浏览量:1230,Q&A: 0
    [Abstract] Almost all functions of cells or organs rely on the activities of cellular enzymes. Indeed, the in-vivo activities that directly represent the cellular effects of enzymes in live organs are critical importance to appreciate the roles enzymes play in modulating physiological or pathological processes, although assessments of such in-vivo ...
    Visualization of Macropinocytosis in Prostate Fibroblasts
    前列腺成纤维细胞中巨胞饮的可视检测
    作者:Rajeev Mishra and Neil A. Bhowmick日期:05/20/2019,浏览量:1582,Q&A: 0
    [Abstract] Macropinocytosis has emerged as an important mechanism for non-selective route to internalize extracellular fluids and dissolved molecules in eukaryotic cell. As fundamental cellular behavior, macropinocytosis plays specific and distinct roles in many physiological and pathological processes, such as nutrients uptake, antigen presentation, ...
    3D Co-culture System of Mouse Prostatic Wild-type Fibroblasts with Human Prostate Cancer Epithelial Cells
    小鼠前列腺野生型成纤维细胞和人前列腺癌上皮细胞的3D共培养系统
    作者:Veronica R. Placencio-Hickok, Manabu Kato and Neil A. Bhowmick日期:05/05/2019,浏览量:912,Q&A: 0
    [Abstract] Heterogeneous prostatic carcinoma-associated fibroblasts (CAF) contribute to tumor progression. This was established using transgenic mouse models. Paracrine interactions between fibroblasts and epithelial cells were further interrogated using isolated 2D cell culture systems, but 3D culture systems currently being developed can better mimic ...
    Measuring Protein Synthesis during Cell Cycle by Azidohomoalanine (AHA) Labeling and Flow Cytometric Analysis
    利用叠氮丙氨酸标记和流式细胞分析测定细胞周期中蛋白的合成
    作者:Koshi Imami and Tomoharu Yasuda日期:04/20/2019,浏览量:1155,Q&A: 0
    [Abstract] Protein synthesis is one of the most fundamental biological processes to maintain cellular proteostasis. Azidohomoalaine (AHA) is a non-radioactive and “clickable” amino acid analog of methionine which can be incorporated into newly synthesized proteins. Thus, AHA-labeled nascent proteins can be detected and quantified through fluorescent labeling ...