癌症生物学

分类

    现刊
    Measuring Cell Growth and Junction Development in Epithelial Cells Using Electric Cell-Substrate Impedance Sensing (ECIS)
    使用电子细胞基质阻抗传感(ECIS)测量上皮细胞生长和连接发育
    作者:Shaista Anwer and Katalin Szaszi日期:08/20/2020,浏览量:925,Q&A: 0
    [Abstract] Electric Cell-substrate Impedance Sensing (ECIS) is an automated method that can be used to quantify processes such as cell attachment, growth, migration and barrier functions (i.e., the properties of tight junctions). The method provides simultaneous information on cell number and tight junction function by detecting electric parameters ...
    SMART (Single Molecule Analysis of Resection Tracks) Technique for Assessing DNA end-Resection in Response to DNA Damage
    SMART(切除轨迹单分子分析)技术用于评估DNA末端切除对DNA损伤的反应
    作者:Angela Altieri, Milena Dell’Aquila, Francesca Pentimalli, Antonio Giordano and Alfano Luigi日期:08/05/2020,浏览量:737,Q&A: 0
    [Abstract] DNA double strand breaks (DSBs) are among the most toxic lesions affecting genome integrity. DSBs are mainly repaired through non-homologous end joining (NHEJ) and homologous recombination (HR). A crucial step of the HR process is the generation, through DNA end-resection, of a long 3′ single-strand DNA stretch, necessary to prime DNA synthesis ...
    A CRISPR Competition Assay to Identify Cancer Genetic Dependencies
    基于CRISPR竞争法鉴定癌症的遗传依赖性
    作者:Vishruth Girish and Jason M. Sheltzer日期:07/20/2020,浏览量:1152,Q&A: 0
    [Abstract] The CRISPR/Cas9 system is a powerful tool for genome editing, wherein the RNA-guided nuclease Cas9 can be directed to introduce double-stranded breaks (DSBs) at a targeted locus. In mammalian cells, these DSBs are typically repaired through error-prone processes, resulting in insertions or deletions (indels) at the targeted locus. Researchers can ...
    Immunofluorescent Staining of Claudin-2 in Cultured Kidney Tubular Cells
    肾脏肾小管培养细胞中Claudin-2的免疫荧光染色
    作者:Shaista Anwer and Katalin Szaszi日期:07/20/2020,浏览量:467,Q&A: 0
    [Abstract] Members of the claudin family of tight junction proteins regulate paracellular permeability and modulate cell signaling. During junction remodeling, these proteins are selectively inserted into or retrieved from the tight junctions, but the control and coordination of these processes remain incompletely understood. Visualization of claudins allows ...
    Tyramide Signal-Amplified Immunofluorescence of MYCN and MYC in Human Tissue Specimens and Cell Line Cultures
    酪胺信号放大的人体组织标本和细胞系培养物中的MYCN和MYC免疫荧光
    作者:Johanna M. Schafer and Jennifer A. Pietenpol日期:07/05/2020,浏览量:685,Q&A: 0
    [Abstract] MYC family members, MYC, MYCN, and MYCL, are oncogenic transcription factors that regulate the expression of genes involved in normal development, cell growth, proliferation, metabolism, and survival. While MYC is amplified and/or overexpressed across a variety of tissue types, MYCN is often overexpressed in tumors of the nervous system ...
    Generation and Testing of Fluorescent Adaptable Simple Theranostic (FAST) Proteins
    FAST蛋白的生成与检测
    [Abstract] This protocol provides a step-by-step method to create recombinant fluorescent fusion proteins that can be secreted from mammalian cell lines. This builds on many other recombinant protein and fluorescent protein techniques, but is among the first to harness fluorescent fusion proteins secreted directly into cell culture supernatant. This opens ...
    NMR waterLOGSY as An Assay in Drug Development Programmes for Detecting Protein-Ligand Interactions–NMR waterLOGSY
    NMR waterLOGSY应用于药物开发项目中蛋白质-配体相互作用的检测
    作者:Carole J. R. Bataille, Terence H. Rabbitts and Timothy D. W. Claridge日期:07/05/2020,浏览量:797,Q&A: 0
    [Abstract] In drug development programmes, multiple assays are needed for the determination of protein-compound interactions and evaluation of potential use in assays with protein-protein interactions. In this protocol we describe the waterLOGSY NMR method for confirming protein-ligand binding events.
    Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis
    哺乳动物细胞脂筏的分离及其脂质组学分析
    作者:Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J. Meikle and Dmitri Sviridov日期:07/05/2020,浏览量:826,Q&A: 0
    [Abstract] Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and ...
    Modeling NOTCH1 driven T-cell Acute Lymphoblastic Leukemia in Mice
    NOTCH1诱导小鼠T细胞急性淋巴细胞白血病模型的建立
    作者:Agnieszka A. Wendorff and Adolfo A. Ferrando日期:05/20/2020,浏览量:1205,Q&A: 0
    [Abstract] T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy that arises from transformation of T-cell primed hematopoietic progenitors. Although T-ALL is a heterogenous and molecularly complex disease, more than 65% of T-ALL patients carry activating mutations in the NOTCH1 gene. The majority of T-ALL–associated ...
    Isolation of Tumor Cells Based on Their Distance from Blood Vessels
    基于与血管距离的肿瘤细胞分离
    作者:Saran Kumar, Husni Sharife, Tirzah Kreisel, Libat Bar-Lev, Myriam Grunewald and Eli Keshet日期:05/20/2020,浏览量:1348,Q&A: 0
    [Abstract] Differential exposure of tumor cells to microenvironmental cues greatly impacts cell phenotypes, raising a need for position based sorting of tumor cells amenable to multiple OMICs and functional analyses. One such key determinant of tumor heterogeneity in solid tumors is its vasculature. Proximity to blood vessels (BVs) profoundly affects tumor ...