原生动物

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    现刊
    Viral Double-Stranded RNA Detection by DNase I and Nuclease S1 digestions in Leishmania parasites
    在利什曼原虫中利用DNase I和 Nuclease S1酶解进行病毒双链RNA鉴定
    作者:Nathalie Isorce and Nicolas Fasel日期:05/05/2020,浏览量:302,Q&A: 0
    [Abstract] Many RNA viruses are found in protozoan parasites. They can be responsible for more serious pathology or treatment failure. For the detection of viral double-stranded RNA (dsRNA), sequence-dependent and -independent methods are available, such as quantitative real-time PCR and immunofluorescence, dot blot, ELISA or sequencing. The technique ...
    Spectrophotometric Assessment of Heme Oxygenase-1 Activity in Leishmania-infected Macrophages
    利什曼原虫感染巨噬细胞中血红素加氧酶-1活性的分光光度法测定
    作者:Moumita Basu, Shriya Saha and Anindita Ukil日期:04/05/2020,浏览量:373,Q&A: 0
    [Abstract] Heme oxygenase-1 (HO-1) is a stress responsive enzyme that metabolizes heme and releases free iron, carbon monoxide (CO), and biliverdin (BV), which rapidly undergoes conversion to bilirubin (BL). Estimation of bilirubin is the basis of HO-1 assay. HO-1 activity is widely employed to determine antioxidant response of cells under different ...
    CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
    诺氏疟原虫CRISPR-Cas9基因编辑
    作者:Franziska Mohring, Melissa N. Hart, Avnish Patel, David A. Baker and Robert W. Moon日期:02/20/2020,浏览量:740,Q&A: 0
    [Abstract] Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. falciparum, P. knowlesi is the only other malaria parasite capable of being maintained in ...
    Surface Plasmon Resonance Analysis of the Protein-protein Binding Specificity Using Autolab ESPIRIT
    利用Autolab ESPIRIT进行表面等离子体共振分析蛋白间结合特异性
    作者:Pragyan Parimita Rath, Gaurav Anand and Shalini Agarwal日期:02/20/2020,浏览量:752,Q&A: 0
    [Abstract] Direct protein-protein interactions are known to regulate a wide range of cellular activities. To understand these contacts one can employ various experimental methods like Dynamic Light Scattering (DLS), Fluorescence Resonance Energy Transfer (FRET), Isothermal titration calorimetry (ITC), Chemical crosslinking, Co-immunoprecipitation (Co-IP), ...
    A Radioactive-free Kinase Inhibitor Discovery Assay Against the Trypanosoma brucei Glycogen Synthase Kinase-3 short (TbGSK-3s)
    一种无放射性布氏锥虫糖原合成酶激酶-3抑制剂探究实验
    作者:Antonia Efstathiou and Despina Smirlis日期:01/20/2020,浏览量:428,Q&A: 0
    [Abstract] The identification of small molecules possessing inhibitory activity in vitro, against a given target kinase, is the first step in the drug discovery process. Herein, we describe a non radioactive protocol using luciferase-based ATP assay for the identification of inhibitors for the short isoform of the Trypanosoma brucei’s ...
    Quantification of Nitric Oxide and Reactive Oxygen Species in Leishmania-infected J774A.1 Macrophages as a Response to the in vitro Treatment with a Natural Product Compound
    感染利什曼原虫J774A.1的巨噬细胞响应体外天然化合物处理的一氧化氮和活性氧定量分析
    作者:Kalliopi Karampetsou, Olga S. Koutsoni and Eleni Dotsika日期:12/05/2019,浏览量:786,Q&A: 0
    [Abstract] Leishmaniasis is a parasitic disease caused by the obligatory intracellular protozoa Leishmania spp. Current therapeutic options are limited and thus, drug discovery against leishmaniasis is very important. Nevertheless, there is a great difficulty to develop therapeutic strategies against the disease because the parasite deploys various ...
    Leishmania Parasite Quantification by Bioluminescence in Murine Models
    在小鼠模型中利用生物发光进行利什曼原虫的定量分析
    作者:Marta Reverte and Nicolas Fasel日期:11/20/2019,浏览量:837,Q&A: 0
    [Abstract] Leishmaniasis remains a major public health problem worldwide with a prevalence of 12 million, an incidence of 1 million persons, and 350 million people being at risk. Murine models have been largely used for studying the host-pathogen relationship and developing effective chemotherapies against Leishmania parasites. Thus, preclinical ...
    In vitro Screening of Antileishmanial Activity of Natural Product Compounds: Determination of IC50, CC50 and SI Values
    天然复合产物抗利什曼原虫活性的体外筛选:IC50、CC50和SI值的测定
    作者:Olga S. Koutsoni, Kalliopi Karampetsou and Eleni Dotsika日期:11/05/2019,浏览量:711,Q&A: 0
    [Abstract] Neglected tropical diseases gain the scientific interest of numerous research programs in an attempt to achieve their effective control or elimination. In this attempt, more cutting-edge public health policies and research are needed for the discovery of new, safer and effective drugs originated from natural products. Here, we describe protocols ...
    Measurement of Acid Ecto-phosphatase Activity in Live Leishmania donovani Parasites
    杜氏利什曼原虫中酸性胞外磷酸酶活性的活体检测
    作者:Amalia Papadaki and Haralabia Boleti日期:10/05/2019,浏览量:1030,Q&A: 0
    [Abstract] Acid ecto-phosphatases are enzymes that hydrolyze phosphomonoesters in the acidic pH range with their active sites facing the extacellular medium. Their activities can be measured in living cells. In bacteria and protozoan pathogens, acid ecto-phosphatases have been associated with the survival of intracellular pathogens within phagocytes through ...
    Eimeria vermiformis Infection Model of Murine Small Intestine
    蠕形艾美耳球虫对小鼠小肠的感染模型
    作者:Patrícia Figueiredo-Campos, Cristina Ferreira, Birte Blankenhaus and Marc Veldhoen日期:12/20/2018,浏览量:1994,Q&A: 0
    [Abstract] Eimeria vermiformis is a tissue specific, intracellular protozoan that infects the murine small intestinal epithelia, which has been widely used as a coccidian model to study mucosal immunology. This mouse infection model is valuable to investigate the mechanisms of host protection against primary and secondary infection in the small ...