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    现刊
    Plasmodium cynomolgi Berok Growth Inhibition Assay by Thiol-reactive Probe Based Flow Cytometric Measurement
    硫醇反应探针流式细胞术检测食蟹猴疟原虫生长抑制
    作者:Jessica Jie Ying Ong, Bruce Russell and Jin-Hee Han日期:09/05/2021,浏览量:632,Q&A: 0
    [Abstract]

    The relapsing malaria species, Plasmodium vivax, is the most widely distributed and difficult-to-treat cause of human malaria. The merozoites of P. vivax preferentially invade ephemeral human CD71+ reticulocytes (nascent reticulocytes), thereby limiting the development of a robust continuous culture in vitro. Fortunately, P. vivax’s sister

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    Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
    植物线粒体核糖体的纯化和低温电镜分析
    作者:Florent Waltz, Philippe Giegé and Yaser Hashem日期:08/05/2021,浏览量:773,Q&A: 0
    [Abstract]

    Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production. Among mitochondrial gene expression processes, translation is the least understood; although, recent advances

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    Fluorescence-based Heme Quantitation in Toxoplasma Gondii
    基于荧光的弓形虫血红素定量
    作者:Amy Bergmann and Zhicheng Dou日期:06/20/2021,浏览量:1065,Q&A: 0
    [Abstract]

    Toxoplasma gondii is a highly prevalent protozoan pathogen throughout the world. As a eukaryotic intracellular pathogen, Toxoplasma ingests nutrients from host cells to support its intracellular growth. The parasites also encode full or partial metabolic pathways for the biosynthesis of certain nutrients, such as heme. Heme is an essential

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    Sex-specific Separation of Plasmodium falciparum Gametocyte Populations
    恶性疟原虫配子细胞群体的性别特异性分离
    作者:Melanie C. Ridgway, Daniela Cihalova and Alexander G. Maier日期:06/05/2021,浏览量:1264,Q&A: 0
    [Abstract]

    Plasmodium falciparum is a unicellular eukaryotic parasite that causes malaria in humans. The parasite is spread by Anopheles mosquitoes after ingestion of sexual stage parasites known as gametocytes. Malaria transmission depends on parasites switching from the disease-causing asexual blood forms to male and female gametocytes. The current

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    Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy
    单粒子低温电子显微镜双态微管馏分的制备
    作者:Corbin Black, Daniel Chen Dai, Katya Peri, Muneyoshi Ichikawa and Khanh Huy Bui日期:06/05/2021,浏览量:1755,Q&A: 0
    [Abstract]

    Over the years, studying the ultrastructure of the eukaryotic cilia/flagella using electron microscopy (EM) has contributed significantly toward our understanding of ciliary function. Major complexes in the cilia, such as inner and outer dynein arms, radial spokes, and dynein regulatory complexes, were originally discovered by EM. Classical

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    Isolation of GFP-expressing Malarial Hypnozoites by Flow Cytometry Cell Sorting
    流式细胞术分离表达GFP的疟原虫催眠素
    作者:Annemarie Voorberg-van der Wel, Sam O. Hoffman and Clemens H. M. Kocken日期:05/05/2021,浏览量:1568,Q&A: 0
    [Abstract]

    Hypnozoites are dormant liver-stage parasites unique to relapsing malarial species, including the important human pathogen Plasmodium vivax, and pose a barrier to the elimination of malaria. Little is known regarding the biology of these stages, largely due to their inaccessible location. Hypnozoites can be cultured in vitro but these cultures

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    Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum
    恶性疟原虫无性期原位蛋白质棕榈酰化的点击化学成像
    作者:Mansoor A. Siddiqui日期:05/05/2021,浏览量:2195,Q&A: 0
    [Abstract]

    Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases (PATs or DHHC-PATs). Palmitoylation has recently emerged as a crucial post-translational

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    Trypanosomatid, fluorescence-based in vitro U-insertion/U-deletion RNA-editing (FIDE)
    基于荧光的锥虫体体外尿嘧啶插入或删除RNA编辑(FIDE)
    作者:Wolf-Matthias Leeder, Elisabeth Kruse and H. Ulrich Göringer日期:03/05/2021,浏览量:1803,Q&A: 0
    [Abstract]

    Gene expression within the mitochondria of African trypanosomes and other protozoan organisms relies on a nucleotide-specific RNA-editing reaction. In the process exclusively uridine (U)-nucleotides are site-specifically inserted into and deleted from sequence-deficient primary transcripts to convert them into translatable mRNAs. The reaction is

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    Plant ARGONAUTE Protein Immunopurification for Pathogen Cross Kingdom Small RNA Analysis
    植物ARGONAUTE蛋白免疫纯化用于病原体跨界小RNA分析
    作者:Florian Dunker, Bernhard Lederer and Arne Weiberg日期:02/05/2021,浏览量:2125,Q&A: 0
    [Abstract]

    Over the last decade, it has been noticed that microbial pathogens and pests deliver small RNA (sRNA) effectors into their host plants to manipulate plant physiology and immunity for infection, known as cross kingdom RNA interference. In this process, fungal and oomycete parasite sRNAs hijack the plant ARGONAUTE (AGO)/RNA-induced silencing complex

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    Determination of Intracellular Ca2+ Concentration in the Human Pathogens Trypanosomatids Leishmania mexicana and Trypanosoma cruzi by the Use of the Fluorescent Ca2+ Indicator Fura-2
    利用荧光Ca2+指示剂Fura-2测定人类病原体锥虫,墨西哥利什曼原虫和克氏锥虫中细胞内Ca2+的浓度
    作者:Andre Rey-Cibati, Mariana Valladares-Delgado and Gustavo Benaim日期:09/20/2020,浏览量:1714,Q&A: 0
    [Abstract] Ca2+ is an essential signaling messenger in all eukariotic cells, playing a pivotal role in many cellular functions as cell growth control (differentiation, fertilization and apoptosis), secretion, gene expression, enzyme regulation, among many others. This basic premise includes trypanosomatids as Trypanosoma cruzi and various ...