真菌

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    现刊
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
    All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the ...
    Preparation of RNA 3’ End Sequencing Libraries of Total and 4-thiouracil Labeled RNA for Simultaneous Measurement of Transcription, RNA Synthesis and Decay in S. cerevisiae
    制备酿酒酵母总RNA和4-硫尿RNA的RNA3'端测序文库用于转录,RNA合成和降解的同步测定
    作者:Manfred Schmid, Agnieszka Tudek and Torben Heick Jensen日期:03/20/2019,浏览量:985,Q&A: 0
    Cellular RNA levels are determined by the rates of RNA transcription from the gene template and subsequent RNA stability. Knowledge about both transcription and RNA decay is, therefore, necessary to interpret RNA levels and gene expression, ...
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
    [Abstract] All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...
    Visualizing Hypoxia in a Murine Model of Candida albicans Infection Using in vivo Biofluorencence
    用活体生物荧光法观察小鼠白念珠菌感染模型中的缺氧情况
    作者:José Pedro Lopes and Constantin F. Urban日期:08/05/2019,浏览量:292,Q&A: 0
    [Abstract] Candida albicans is a leading human fungal pathogen that uses several metabolic adaptations to escape immune cells and causes systemic disease. Here, we describe a protocol for measuring one of these adaptations, the ability to thrive in hypoxic niches. Hypoxia was generated after successful subdermal infection with C. albicans ...
    QUEEN-based Spatiotemporal ATP Imaging in Budding and Fission Yeast
    发芽酵母和裂殖酵母中基于QUEEN的时空ATP成像
    作者:Masak Takaine日期:08/05/2019,浏览量:246,Q&A: 0
    [Abstract] Yeasts have provided an exceptional model for studying metabolism and bioenergetics in eukaryotic cells. Among numerous metabolites, adenosine triphosphate (ATP) is a major metabolite that is essential for all living organisms. Therefore, a clearer understanding of ATP dynamics in living yeast cells is important for deciphering cellular energy ...
    Endocytosis Detection in Magnaporthe oryzae
    稻瘟病菌内吞作用的检测
    作者:Muxing Liu and Zhengguang Zhang日期:08/05/2019,浏览量:188,Q&A: 0
    [Abstract] Endocytosis is an intracellular trafficking pathway that occurs in nutrient uptake, signal transduction and reconstruction of cell polarity and is conserved in eukaryotic cells. In fungi, endocytosis plays crucial roles in the physiology of hyphal growth and pathogenicity. vidence for endocytosis in filamentous fungi is detected by the ...
    Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association
    蓝甲醇衍生物作为丛枝菌根真菌与植物共生标志物的定量研究
    作者:Elisabeth Mindt, Ming Wang, Martin Schäfer, Rayko Halitschke and Ian T. Baldwin日期:07/20/2019,浏览量:616,Q&A: 0
    [Abstract] Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, ...
    Isolation of Powdery Mildew Haustoria from Infected Barley
    感染大麦中白粉病吸器的分离提取
    作者:Linhan Li, Benjamin Collier and Pietro D. Spanu日期:07/20/2019,浏览量:579,Q&A: 0
    [Abstract] Blumeria graminis is a fungus that causes powdery mildews on grasses, such as barley. Investigations of this pathogen present many challenges due to its obligate biotrophic nature. This means that the fungus can only grow in the presence of a living host plant. B. graminis forms epiphytic mycelia on the plant surface and feeding ...
    A Protocol to Map the Spatial Proteome Using HyperLOPIT in Saccharomyces cerevisiae
    利用HyperLOPIT绘制酿酒酵母空间蛋白质组图谱的方法
    作者:Daniel J. H. Nightingale, Kathryn S. Lilley and Stephen G. Oliver日期:07/20/2019,浏览量:542,Q&A: 0
    [Abstract] The correct subcellular localization of proteins is vital for cellular function and the study of this process at the systems level will therefore enrich our understanding of the roles of proteins within the cell. Multiple methods are available for the study of protein subcellular localization, including fluorescence microscopy, organelle ...
    Imaging Gene Expression Dynamics in Pseudomonas fluorescens In5 during Interactions with the Fungus Fusarium graminearum PH-1
    荧光假单胞菌In5与小麦赤霉菌PH-1互作过程中的基因表达动态成像
    作者:Rosanna C. Hennessy, Peter Stougaard and Stefan Olsson日期:06/20/2019,浏览量:588,Q&A: 0
    [Abstract] Genomics, transcriptomics and metabolomics are powerful technologies for studying microbial interactions. The main drawback of these methods is the requirement for destructive sampling. We have established an alternative but complementary technique based on a microplate system combined with promoter fusions for visualizing gene expression in space ...
    Preparation of RNA 3’ End Sequencing Libraries of Total and 4-thiouracil Labeled RNA for Simultaneous Measurement of Transcription, RNA Synthesis and Decay in S. cerevisiae
    制备酿酒酵母总RNA和4-硫尿RNA的RNA3'端测序文库用于转录,RNA合成和降解的同步测定
    作者:Manfred Schmid, Agnieszka Tudek and Torben Heick Jensen日期:03/20/2019,浏览量:985,Q&A: 0
    [Abstract] Cellular RNA levels are determined by the rates of RNA transcription from the gene template and subsequent RNA stability. Knowledge about both transcription and RNA decay is, therefore, necessary to interpret RNA levels and gene expression, especially during cellular processes where these parameters change. Numerous experimental strategies have ...
    I Plate-based Assay for Studying How Fungal Volatile Compounds (VCs) Affect Plant Growth and Development and the Identification of VCs via SPME-GC-MS
    用于研究真菌挥发性化合物(VCs)如何影响植物生长发育并通过SPME-GC-MS技术鉴定VCs的I板检测法
    作者:Wenzhao Wang, Ningxiao Li, Xingzhong Liu and Seogchan Kang日期:02/20/2019,浏览量:1439,Q&A: 0
    [Abstract] Biogenic volatile compounds (VCs) mediate various types of crucial intra- and inter-species interactions in plants, animals, and microorganisms owing to their ability to travel through air, liquid, and porous soils. To study how VCs produced by Verticillium dahliae, a soilborne fungal pathogen, affect plant growth and development, we ...