病毒

分类

    现刊
    Direct Reprogramming of Mouse Embryonic Fibroblasts to Conventional Type 1 Dendritic Cells by Enforced Expression of Transcription Factors
    转录因子强化表达直接将小鼠胚胎成纤维细胞重编程为常规1型树突状细胞
    作者:Fábio F. Rosa, Cristiana F. Pires, Olga Zimmermannova and Carlos-Filipe Pereira日期:05/20/2020,浏览量:333,Q&A: 0
    Ectopic expression of transcription factor combinations has been recently demonstrated to reprogram differentiated somatic cells towards the dendritic cell (DC) lineage without reversion to a multipotent state. DCs have the ability to induce potent ...
    Direct Reprogramming of Mouse Embryonic Fibroblasts to Conventional Type 1 Dendritic Cells by Enforced Expression of Transcription Factors
    转录因子强化表达直接将小鼠胚胎成纤维细胞重编程为常规1型树突状细胞
    作者:Fábio F. Rosa, Cristiana F. Pires, Olga Zimmermannova and Carlos-Filipe Pereira日期:05/20/2020,浏览量:333,Q&A: 0
    [Abstract] Ectopic expression of transcription factor combinations has been recently demonstrated to reprogram differentiated somatic cells towards the dendritic cell (DC) lineage without reversion to a multipotent state. DCs have the ability to induce potent and long-lasting adaptive immune responses. In particular, conventional type 1 DCs (cDC1s) excel on ...
    Split Nano Luciferase-based Assay to Measure Assembly of Japanese Encephalitis Virus
    基于分割纳米荧光素酶的乙型脑炎病毒组装检测
    作者:Simon Goto, Kotaro Ishida, Ryosuke Suzuki and Eiji Morita日期:05/05/2020,浏览量:287,Q&A: 0
    [Abstract] Cells infected with flavivirus release various forms of infectious and non-infectious particles as products and by-products. Comprehensive profiling of the released particles by density gradient centrifugation is informative for understanding viral particle assembly. However, it is difficult to detect low-abundance minor particles in such ...
    Viral Double-Stranded RNA Detection by DNase I and Nuclease S1 digestions in Leishmania parasites
    在利什曼原虫中利用DNase I和 Nuclease S1酶解进行病毒双链RNA鉴定
    作者:Nathalie Isorce and Nicolas Fasel日期:05/05/2020,浏览量:302,Q&A: 0
    [Abstract] Many RNA viruses are found in protozoan parasites. They can be responsible for more serious pathology or treatment failure. For the detection of viral double-stranded RNA (dsRNA), sequence-dependent and -independent methods are available, such as quantitative real-time PCR and immunofluorescence, dot blot, ELISA or sequencing. The technique ...
    Enzymatic Construction of Protein Polymer/Polyprotein Using OaAEP1 and TEV Protease
    利用OaAEP1和TEV蛋白酶进行蛋白聚合物/多聚蛋白的酶促构建
    作者:Yibing Deng, Shengchao Shi, Bin Zheng, Tao Wu and Peng Zheng日期:04/20/2020,浏览量:762,Q&A: 0
    [Abstract] The development of chemical and biological coupling technologies in recent years has made possible of protein polymers engineering. We have developed an enzymatic method for building polyproteins using a protein ligase OaAEP1 (asparagine endopeptidase 1) and protease TEV (tobacco etching virus). Using a mobile TEV protease site compatible with the ...
    Environmental Conditioning and Aerosol Infection of Mice
    环境调节与小鼠气溶胶感染
    作者:Eriko Kudo and Akiko Iwasaki日期:04/20/2020,浏览量:486,Q&A: 0
    [Abstract] Influenza infection models in mice are widely used to study flu-mediated immune responses and pathology. However, most laboratory mice are housed at 20 °C and 50% relative humidity (RH). To better recapitulate influenza epidemics and immune responses during winter seasons, mice were housed at 20 °C under different humidity conditions, 10-20% or ...
    A Triple-challenge Mouse Model of Allergic Airway Disease, Primary Influenza Infection, and Secondary Bacterial Infection
    气道变态反应性疾病,原发性流感感染,继发性细菌感染的三重小鼠模型
    作者:Sean Roberts, Clare M Williams, Sreeja Roy and Yoichi Furuya日期:04/20/2020,浏览量:417,Q&A: 0
    [Abstract] Asthma is a global problem that affects millions of individuals. An increased risk of respiratory viral and bacterial infections is one of the complications of asthma. We recently reported that mice with ovalbumin-induced allergic airway disease (AAD) are protected against influenza-Streptococcus pneumoniae co-infection. Here, we describe ...
    Preparation of a Bacteriophage T4-based Prokaryotic-eukaryotic Hybrid Viral Vector for Delivery of Large Cargos of Genes and Proteins into Human Cells
    用于将基因和蛋白质转运至人体细胞中的基于噬菌体T4的大容量原核真核杂交病毒载体的制备
    作者:Jingen Zhu, Pan Tao, Marthandan Mahalingam and Venigalla B. Rao日期:04/05/2020,浏览量:353,Q&A: 0
    [Abstract] A viral vector that can safely and efficiently deliver large and diverse molecular cargos into cells is the holy grail of curing many human diseases. Adeno-associated virus (AAV) has been extensively used but has a very small capacity. The prokaryotic virus T4 has a large capacity but lacks natural mechanisms to enter mammalian cells. Here, we ...
    Real-time Fluorescence Measurement of Enterovirus Uncoating
    肠道病毒脱壳的实时荧光检测
    作者:Visa Ruokolainen, Mira Laajala and Varpu Marjomäki日期:04/05/2020,浏览量:308,Q&A: 0
    [Abstract] Viruses need to open, i.e., uncoat, in order to release their genomes for efficient replication and translation. Especially for non-enveloped viruses, such as enteroviruses, the cues leading to uncoating are less well known. The status of the virus has previously been observed mainly by transmission electron microscopy using negative ...
    Purification of Rice Stripe Virus
    水稻条纹病毒纯化
    作者:Gang Lu, Min Yao, Yijun Zhou and Xiaorong Tao日期:03/20/2020,浏览量:445,Q&A: 0
    [Abstract] Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice tissues. RSV virions with high infectivity were first precipitated ...
    Photoactivable Cholesterol as a Tool to Study Interaction of Influenza Virus Hemagglutinin with Cholesterol
    利用光活化胆固醇研究流感病毒血凝素与胆固醇相互作用
    作者:Bodan Hu, Mohamed Rasheed Gadalla, Christoph Thiele and Michael Veit日期:02/20/2020,浏览量:700,Q&A: 0
    [Abstract] Non-covalent binding of cholesterol to the transmembrane region of proteins affect their functionalities, but methods to prove such an interaction are rare. We describe our protocol to label the hemagglutinin (HA) of Influenza virus with a cholesterol derivative in living cells or with immunoprecipitated protein. We synthesized a “clickable” ...