病毒

分类

    现刊
    Viral Chromosome Conformation Capture (V3C) Assays for Identifying Trans-interaction Sites between Lytic Viruses and the Cellular Genome
    病毒染色体构象俘获技术用于裂解病毒和细胞基因组之间反向相互作用位点的检测
    作者:Kinjal Majumder, Maria Boftsi and David J Pintel日期:03/20/2019,浏览量:868,Q&A: 0
    The folding mechanisms of the mammalian genome package our genetic material into the nucleus, and in doing so, dictate its appropriate replication and expression. Chromosome conformation capture technology has enabled the dissection of the folding ...
    Image-based Quantification of Direct Cell-to-cell Transmission of Bovine Viral Diarrhea Virus
    基于成像量化分析牛病毒性腹泻病毒的细胞间传播
    作者:Fernando Merwaiss and Diego E. Alvarez日期:08/05/2019,浏览量:189,Q&A: 0
    [Abstract] Different viruses rely on direct cell-to-cell transmission to propagate infection within the infected host. Measuring this mode of transmission in cultured cells is often complicated by the contribution of cell free viruses to spread, and the difficulty to distinguish between primary infected cells that produce the virus and neighboring cells that ...
    Fluorescence Microscopy Assay to Measure HIV-1 Capsid Uncoating Kinetics in vitro
    HIV-1衣壳脱壳动力学的荧光显微体外检测
    [Abstract] The stability of the HIV-1 capsid and the spatiotemporal control of its disassembly, a process called uncoating, need to be finely tuned for infection to proceed. Biochemical methods for measuring capsid lattice disassembly in bulk are unable to resolve intermediates in the uncoating reaction. We have developed a single-particle fluorescence ...
    In vitro Infection of Primary Human Monocytes with HIV-1
    原代人单核细胞的体外HIV-1侵染
    作者:Patrick O’Connell, Yong-Hui Zheng, Andrea Amalfitano and Yasser A. Aldhamen日期:07/05/2019,浏览量:493,Q&A: 0
    [Abstract] Monocyte infection by HIV-1 is an important component of chronic HIV pathogenesis. Following infection by HIV-1, monocytes are able to cross the blood brain barrier and set up a viral reservoir in the central nervous system. Additionally, in the setting of chronic HIV-1 infection, monocytes can become activated either directly through HIV-1 ...
    Detachment Procedure of Bacteria from Atmospheric Particles for Flow-cytometry Counting
    从大气颗粒物中分离细菌用于流式细胞计数的步骤
    作者:Carolina M. Araya, Alberto Cazorla and Isabel Reche日期:06/20/2019,浏览量:583,Q&A: 0
    [Abstract] The protocol separates bacteria from atmospheric particles, obtaining with greater precision their abundance in the atmospheric deposition. This procedure is similar to the one used to separate bacteria in streambed sediments. The detachment procedure consists of a chemical treatment with sodium pyrophosphate and Tween 20 and a physical treatment ...
    Production and Purification of Dengue Virus-like Particles from COS-1 Cells
    从COS-1细胞中制备和纯化登革热病毒样颗粒
    作者:Jedhan Ucat Galula, Gwong-Jen J. Chang and Day-Yu Chao日期:06/20/2019,浏览量:606,Q&A: 0
    [Abstract] Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires abundant, and high-purity production methods. ...
    Analysis of Functional Virus-generated PAMP RNAs Using IFNα/β ELISA Assay
    利用干扰素α/β酶联免疫吸附试验检测分析病毒产生的功能性PAMP RNAs
    作者:Margit Mutso, Xiang Liu, Andres Merits and Suresh Mahalingam日期:06/20/2019,浏览量:434,Q&A: 0
    [Abstract] Virus-generated PAMP RNAs are key factors that activate host immune response. The PAMP RNAs are therefore usually closely related with viral disease pathogenesis. Quantitative real time PCR is a conventional method to assess RNA. However, it cannot be used for detecting short dsRNAs generated by viral replicase. This protocol was established to ...
    Enterovirus Competition Assay to Assess Replication Fitness
    用于检测复制适应性的肠道病毒竞争实验
    作者:Valeria Lulla and Andrew E. Firth日期:05/20/2019,浏览量:603,Q&A: 0
    [Abstract] In virology the difference between the fitness of two viruses can be determined by using various methods, such as virus titer, growth curve analysis, measurement of virus infectivity, analysis of produced RNA copies and viral protein production. However, for closely performing viruses, it is often very hard to distinguish the differences. In vitro ...
    TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4+ T Cells
    TZA, 一种基于敏感报告因子细胞学检测方法, 可用于准确快速量化静息CD4+ T细胞中可诱导的且复制能力强的潜伏HV-1
    作者:Anwesha Sanyal, Vatsala S. Rangachar and Phalguni Gupta日期:05/20/2019,浏览量:960,Q&A: 1
    [Abstract] The latent HIV-1 viral reservoir in resting CD4+ (rCD4+) T cells represents a major barrier to an HIV-1 cure. There is an ongoing effort to identify therapeutic approaches that will eliminate or reduce the size of this reservoir. However, clinical investigators lack an assay to determine whether or not a decrease in the ...
    Purification and Proteomic Analysis of Alphavirus Particles from Sindbis Virus Grown in Mammalian and Insect Cells
    哺乳动物和昆虫细胞中的辛德比斯病毒的α病毒颗粒的纯化和蛋白质组分析
    作者:Raquel Hernandez, Trevor Glaros, Gabrielle Rizzo and Davis F. Ferreira日期:05/20/2019,浏览量:741,Q&A: 0
    [Abstract] Current mass spectrometry (MS) methods and new instrumentation now allow for more accurate identification of proteins in low abundance than previous protein fractionation and identification methods. It was of interest if this method could serve to define the virus proteome of a membrane-containing virus. To evaluate the efficacy of mass spec to ...
    A Flow Cytometric Method to Determine Transfection Efficiency
    利用流式细胞术测定转染效率
    [Abstract] Mammalian cell transfection is a powerful technique commonly used in molecular biology to express exogenous DNA or RNA in cells and study gene and protein function. Although several transfection strategies have been developed, there is a wide variation with regards to transfection efficiency, cell toxicity and reproducibility. Thus, a sensitive ...