细胞生物学

分类

    现刊
    An in vitro DNA Sensor-based Assay to Measure Receptor-specific Adhesion Forces of Eukaryotic Cells and Pathogens
    基于DNA传感器的真核细胞与病原体受体特异性粘附力的体外检测
    作者:Maurizio Wack, Tina Wiegand, Friedrich Frischknecht and E. Ada Cavalcanti-Adam日期:09/05/2020,浏览量:710,Q&A: 0
    Motility of eukaryotic cells or pathogens within tissues is mediated by the turnover of specific interactions with other cells or with the extracellular matrix. Biophysical characterization of these ligand-receptor adhesions helps to unravel the ...
    Fluorescent Polysome Profiling in Caenorhabditis elegans
    秀丽隐杆线虫荧光多聚体分析
    作者:Dan Shaffer and Jarod A Rollins日期:09/05/2020,浏览量:545,Q&A: 0
    An important but often overlooked aspect of gene regulation occurs at the level of protein translation. Many genes are regulated not only by transcription but by their propensity to be recruited to actively translating ribosomes (polysomes). ...
    Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells
    细胞系和原代免疫细胞中的低水平活性氧的流式细胞仪检测
    作者:Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd日期:09/05/2020,浏览量:904,Q&A: 0
    Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst are used to fight pathogens, low to moderate ...
    Quantitative Kinetic Analyses of Histone Turnover Using Imaging and Flow Cytometry
    组蛋白转换的定量动力学成像及流式细胞术研究
    作者:Hanae Sato, Robert H. Singer and John M. Greally日期:09/05/2020,浏览量:608,Q&A: 0
    Dynamic histone changes occur as a central part of chromatin regulation. Deposition of histone variants and post-translational modifications of histones are strongly associated with properties of chromatin status. Characterizing the kinetics of ...
    Live-cell Imaging by Super-resolution Confocal Live Imaging Microscopy (SCLIM): Simultaneous Three-color and Four-dimensional Live Cell Imaging with High Space and Time Resolution
    超分辨率共聚焦显微镜活体细胞成像(SCLIM):高时空分辨率的三色、四维同步活体细胞成像
    作者:Kazuo Kurokawa and Akihiko Nakano日期:09/05/2020,浏览量:674,Q&A: 0
    Many questions in cell biology can be solved by state-of-the-art technology of live cell imaging. One good example is the mechanism of membrane traffic, in which small membrane carriers are rapidly moving around in the cytoplasm to deliver cargo ...
    Multiplication and Growth Inhibition Activity Assays for the Zoonotic Malaria Parasite, Plasmodium knowlesi
    人畜共患病疟原虫——诺氏疟原虫增殖和生长抑制活性测定
    作者:Franziska Mohring, Thomas A. Rawlinson, Simon J. Draper and Robert W. Moon日期:09/05/2020,浏览量:445,Q&A: 0
    Malaria remains a major cause of morbidity and mortality globally. Clinical symptoms of the disease arise from the growth and multiplication of Plasmodium parasites within the blood of the host. Thus in vitro assays to determine ...
    Integration of Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons into Rat Brain
    人诱导多能干细胞(hiPSC)源神经元与大鼠脑回路的整合
    作者:Xiling Yin, Ted M. Dawson and Valina L. Dawson日期:09/05/2020,浏览量:639,Q&A: 0
    Human neuron transplantation offers novel opportunities for modeling human neurologic diseases and potentially replacement therapies. However, the complex structure of the human cerebral cortex, which is organized in six layers with tightly ...
    An in vitro DNA Sensor-based Assay to Measure Receptor-specific Adhesion Forces of Eukaryotic Cells and Pathogens
    基于DNA传感器的真核细胞与病原体受体特异性粘附力的体外检测
    作者:Maurizio Wack, Tina Wiegand, Friedrich Frischknecht and E. Ada Cavalcanti-Adam日期:09/05/2020,浏览量:710,Q&A: 0
    [Abstract] Motility of eukaryotic cells or pathogens within tissues is mediated by the turnover of specific interactions with other cells or with the extracellular matrix. Biophysical characterization of these ligand-receptor adhesions helps to unravel the molecular mechanisms driving migration. Traction force microscopy or optical tweezers are typically ...
    Fluorescent Polysome Profiling in Caenorhabditis elegans
    秀丽隐杆线虫荧光多聚体分析
    作者:Dan Shaffer and Jarod A Rollins日期:09/05/2020,浏览量:545,Q&A: 0
    [Abstract] An important but often overlooked aspect of gene regulation occurs at the level of protein translation. Many genes are regulated not only by transcription but by their propensity to be recruited to actively translating ribosomes (polysomes). Polysome profiling allows for the separation of unbound 40S and 60S subunits, 80S monosomes, and actively ...
    Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells
    细胞系和原代免疫细胞中的低水平活性氧的流式细胞仪检测
    作者:Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd日期:09/05/2020,浏览量:904,Q&A: 0
    [Abstract] Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst are used to fight pathogens, low to moderate amounts of ROS act as signaling molecules important for several physiological processes such as regulation ...
    Quantitative Kinetic Analyses of Histone Turnover Using Imaging and Flow Cytometry
    组蛋白转换的定量动力学成像及流式细胞术研究
    作者:Hanae Sato, Robert H. Singer and John M. Greally日期:09/05/2020,浏览量:608,Q&A: 0
    [Abstract] Dynamic histone changes occur as a central part of chromatin regulation. Deposition of histone variants and post-translational modifications of histones are strongly associated with properties of chromatin status. Characterizing the kinetics of histone variants allows important insights into transcription regulation, chromatin maintenance and ...
    Live-cell Imaging by Super-resolution Confocal Live Imaging Microscopy (SCLIM): Simultaneous Three-color and Four-dimensional Live Cell Imaging with High Space and Time Resolution
    超分辨率共聚焦显微镜活体细胞成像(SCLIM):高时空分辨率的三色、四维同步活体细胞成像
    作者:Kazuo Kurokawa and Akihiko Nakano日期:09/05/2020,浏览量:674,Q&A: 0
    [Abstract] Many questions in cell biology can be solved by state-of-the-art technology of live cell imaging. One good example is the mechanism of membrane traffic, in which small membrane carriers are rapidly moving around in the cytoplasm to deliver cargo proteins between organelles. For directly visualizing the events in membrane trafficking system, ...
    Multiplication and Growth Inhibition Activity Assays for the Zoonotic Malaria Parasite, Plasmodium knowlesi
    人畜共患病疟原虫——诺氏疟原虫增殖和生长抑制活性测定
    作者:Franziska Mohring, Thomas A. Rawlinson, Simon J. Draper and Robert W. Moon日期:09/05/2020,浏览量:445,Q&A: 0
    [Abstract] Malaria remains a major cause of morbidity and mortality globally. Clinical symptoms of the disease arise from the growth and multiplication of Plasmodium parasites within the blood of the host. Thus in vitro assays to determine how drug, antibody and genetic perturbations affect the growth rate of Plasmodium parasites ...
    Integration of Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons into Rat Brain
    人诱导多能干细胞(hiPSC)源神经元与大鼠脑回路的整合
    作者:Xiling Yin, Ted M. Dawson and Valina L. Dawson日期:09/05/2020,浏览量:639,Q&A: 0
    [Abstract] Human neuron transplantation offers novel opportunities for modeling human neurologic diseases and potentially replacement therapies. However, the complex structure of the human cerebral cortex, which is organized in six layers with tightly interconnected excitatory and inhibitory neuronal networks, presents significant challenges for in vivo ...
    Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-κB Reporter Mice
    LPS诱导NF-κB报告小鼠局部炎症及药物沉积的实时体内显像研究
    作者:Artur Schmidtchen and Manoj Puthia日期:08/20/2020,浏览量:804,Q&A: 0
    [Abstract] Wound, biomaterial, and surgical infections are all characterized by a localized and excessive inflammation, motivating the development of in vivo methods focused on the analysis of local immune events. However, current inflammation models, such as the commonly used in vivo models of endotoxin-induced inflammation are based on ...
    Assessing Gαq/15-signaling with IP-One: Single Plate Transfection and Assay Protocol for Cell-Based High-Throughput Assay
    使用IP-One评估Gαq/ 15信号:基于细胞高通量测定的单板转染和测定方案
    作者:Élie Besserer-Offroy, Rebecca L Brouillette, Jean-Michel Longpré and Philippe Sarret日期:08/20/2020,浏览量:632,Q&A: 0
    [Abstract] Cell-based functional assays are an important part of compound screening and drug lead optimization, and they can also play a crucial role in the determination of the residues involved in ligand binding and signaling for a particular G-protein-coupled receptor. Conventional methods used for Gαq/15-coupled receptors rely on the use of ...
    In vitro Cultivation and Visualization of Malaria Liver Stages in Primary Simian Hepatocytes
    猴原代肝细胞体外培养及疟疾肝期观察
    作者:Devendra Kumar Gupta and Thierry Diagana日期:08/20/2020,浏览量:576,Q&A: 0
    [Abstract] Human liver is the primary and obligatory site for malaria infection where sporozoites invade host hepatocytes. Malaria hepatic stages are asymptomatic and represent an attractive target for development of anti-malarial interventions and vaccines. However, owing to lack of robust and reproducible in vitro culture system, it is difficult ...