细胞生物学

分类

    现刊
    Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes
    大鼠肾脏单纯线粒体的分离和线粒体呼吸链复合物蛋白质免疫印迹
    Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell ...
    Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices
    反义寡脱氧核苷酸灌注可阻突触可塑性相关蛋白在海马切片上的基因表达且无诱导补偿作用
    The elucidation of the molecular mechanisms of long-term synaptic plasticity has been hindered by both the compensation that can occur after chronic loss of the core plasticity molecules and by ex vivo conditions that may not reproduce in ...
    3D Organoid Formation from the Murine Salivary Gland Cell Line SIMS
    小鼠唾液腺SIMS细胞系的三维类器官形成
    作者:Harleen K. Athwal and Isabelle M. A. Lombaert日期:10/05/2019,浏览量:148,Q&A: 0
    Salivary glands consist of multiple phenotypically and functionally unique cell populations, such as the acinar, ductal, and myoepithelial cells that help produce, modify, and secrete saliva (Lombaert et al., 2011). Identification of ...
    Reconstituting Breast Tissue with Organotypic Three-dimensional Co-culture of Epithelial and Stromal Cells in Discontinuous Extracellular Matrices
    通过不连续细胞外基质中上皮细胞和基质细胞三维共培养重建乳腺组织
    作者:Gang Ren, Vandana Sharma, Joshua Letson, Yashna Walia, Veani Fernando and Saori Furuta日期:10/05/2019,浏览量:209,Q&A: 0
    Co-culture systems utilizing reconstituted or synthetic extracellular matrix (ECM) and micropatterning techniques have enabled the reconstruction of surface epithelial tissues. This technique has been utilized in the regeneration, disease modeling ...
    Measurement of Acid Ecto-phosphatase Activity in Live Leishmania donovani Parasites
    杜氏利什曼原虫中酸性胞外磷酸酶活性的活体检测
    作者:Amalia Papadaki and Haralabia Boleti日期:10/05/2019,浏览量:111,Q&A: 0
    Acid ecto-phosphatases are enzymes that hydrolyze phosphomonoesters in the acidic pH range with their active sites facing the extacellular medium. Their activities can be measured in living cells. In bacteria and protozoan pathogens, acid ...
    Imaging the Vasculature of Immunodeficient Mice Using Positron Emission Tomography/Computed Tomography (PET/CT) and 18F-fluorodeoxyglucose Labeled Human Erythrocytes
    利用正电子发射断层扫描/计算机断层扫描(PET、CT)和18F-氟脱氧葡萄糖标记人类红细胞 进行免疫缺陷小鼠脉管系统成像
    作者:Shaowei Wang and Jung W. Choi日期:10/05/2019,浏览量:109,Q&A: 0
    Nuclear blood pool imaging using radiolabeled red blood cells has been used in the clinical setting for the evaluation of a number of medical conditions including gastrointestinal hemorrhage, impaired cardiac contractility, and altered ...
    A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-based Scaffolds (3D-dCBS)
    一种获得去细胞牛脊髓细胞外基质构成支架的新方法
    作者:Yavuz E. Arslan, Burcu Efe and Tugba Sezgin Arslan日期:10/05/2019,浏览量:145,Q&A: 0
    Extracellular matrix (ECM)-based tissue engineering scaffolds have an essential role in promoting tissue regeneration. Nerve tissue engineering aims at facilitating the repair of permanent damage to the peripheral and central nervous systems, which ...
    Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal Mouse Macrophages by Flow Cytometry
    利用流式细胞术对巯基乙醇酸盐诱导的小鼠腹腔巨噬细胞中LPS-TLR4复合物内化的定量分析
    作者:Katharina Richard, Stefanie N. Vogel and Darren J. Perkins日期:09/20/2019,浏览量:207,Q&A: 0
    Internalization of cell surface Toll-like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using ...
    Conjugation of Fab’ Fragments with Fluorescent Dyes for Single-molecule Tracking on Live Cells
    Fab片段结合荧光染料用于活细胞单分子示踪
    作者:I-Ting Teng, Xiangning Bu and Inhee Chung日期:09/20/2019,浏览量:241,Q&A: 0
    Our understanding of the regulation and functions of cell-surface proteins has progressed rapidly with the advent of advanced optical imaging techniques. In particular, single-molecule tracking (SMT) using bright fluorophores conjugated to ...
    SIRF: A Single-cell Assay for in situ Protein Interaction with Nascent DNA Replication Forks
    SIRF:一种蛋白与新生DNA复制叉原位相互作用的单细胞检测办法
    作者:Sunetra Roy and Katharina Schlacher日期:09/20/2019,浏览量:300,Q&A: 0
    The duplication of DNA is a fundamental process that is required for the transfer of the genetic information from parent to daughter cells. Aberrant DNA replication processes are associated with diverse disease phenotypes, including developmental ...
    Subcellular Fractionation of Hela Cells for Lysosome Enrichment Using a Continuous Percoll-density Gradient
    利用连续percoll密度梯度富集hela细胞溶酶体亚细胞组分
    作者:Julian M. Carosi, Kathryn J. Hattersley, Yi Cui, Zhe Yang, Rohan D. Teasdale and Timothy J. Sargeant日期:09/20/2019,浏览量:351,Q&A: 0
    The enrichment of lysosomes is a useful way to study their structure and function. These dynamic vesicles can be enriched from cell cultures in a variety of ways including immunoprecipitation and fluorescence-activated organelle sorting. These ...
    Supported Cell Membrane Sheets to Monitor Protein Assembly
    蛋白质组装的细胞膜片监测
    作者:Simon Erlendsson, Thor Seneca Thorsen and Kenneth Lindegaard Madsen日期:09/20/2019,浏览量:251,Q&A: 0
    Studying protein-protein and protein-lipid interactions in their native environment is highly desirable, yet, the heterogeneity and complexity of cellular systems limits the repertoire of experimental methods available. In cells, interactions are ...
    Characterization of Biological Motion Using Motion Sensing Superpixels
    基于运动传感超像素的生物运动特性研究
    作者:Felix Y. Zhou, Carlos Ruiz-Puig, Richard P. Owen, Michael J. White, Jens Rittscher and Xin Lu日期:09/20/2019,浏览量:239,Q&A: 0
    Precise spatiotemporal regulation is the foundation for the healthy development and maintenance of living organisms. All cells must correctly execute their function in the right place at the right time. Cellular motion is thus an important dynamic ...
    In situ Hybridization of Plant-parasitic Nematode Globodera pallida Juveniles to Detect Gene Expression
    原位杂交检测马铃薯白胞囊线虫的基因表达
    作者:Joanna Kud, Nejra Solo, Allan Caplan, Joseph C. Kuhl, Louise-Marie Dandurand and Fangming Xiao日期:09/20/2019,浏览量:239,Q&A: 0
    In this study, we describe a standard whole mount in situ hybridization method which is used to determine the spatial-temporal expression pattern of genes from Globodera spp. Unlike more invasive radioactive labeling approaches, ...
    Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation
    小鼠胚胎脊髓的组织块培养和体外电穿孔介导的基因转移
    Developing axons change responsiveness to guidance cues during the journey to synapse with target cells. Axon crossing at the ventral midline serves as a model for studying how axons accomplish such a switch in their response. Although primary ...
    A Cell Culture Model that Mimics Physiological Tissue Oxygenation Using Oxygen-permeable Membranes
    一种利用透氧膜模拟生理组织氧合的细胞培养模式
    作者:Chloe-Anne Martinez, Peter A. Cistulli and Kristina M. Cook日期:09/20/2019,浏览量:293,Q&A: 0
    Dissolved oxygen and its availability to cells in culture is an overlooked variable which can have significant consequences on experimental research outcomes, including reproducibility. Oxygen sensing pathways play key roles in cell growth and ...
    Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria
    革兰阴性致病菌自然分泌的外-内膜囊泡低温透射电子显微镜观察
    作者:Lidia Delgado, Nicolás Baeza, Carla Pérez-Cruz, Carmen López-Iglesias and Elena Mercadé日期:09/20/2019,浏览量:182,Q&A: 0
    A protocol was developed to visualize and analyze the structure of membrane vesicles (MVs) from Gram-negative bacteria. It is now accepted that these micrometric spherical vesicles are commonly produced by cells from all three domains of life, so ...
    A High-throughput and Pathophysiologically Relevant Astrocyte-motor Neuron Co-culture Assay for Amyotrophic Lateral Sclerosis Therapeutic Discovery
    高通量病理生理相关星形胶质细胞运动神经元共培养技术在肌萎缩侧索硬化治疗中的应用
    作者:Matthew J. Stopford, Scott P. Allen and Laura Ferraiuolo日期:09/05/2019,浏览量:621,Q&A: 0
    Amyotrophic lateral sclerosis (ALS) is an adult onset neurological disorder characterized by loss of motor neurons leading to progressive muscle wasting and eventually death. Astrocytes play a key role in disease pathogenesis. However, the ability ...
    Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells
    人干细胞源性神经元与少突胶质祖细胞的共培养
    作者:Stephanie Dooves, Aishwarya G. Nadadhur, Lisa Gasparotto and Vivi M. Heine日期:09/05/2019,浏览量:575,Q&A: 0
    Crosstalk between neurons and oligodendrocytes is important for proper brain functioning. Multiple co-culture methods have been developed to study oligodendrocyte maturation, myelination or the effect of oligodendrocytes on neurons. However, most of ...
    High Resolution Respirometry in Candida albicans
    白色念珠菌高分辨率呼吸测定
    作者:Lucian Duvenage, Carol A. Munro and Campbell W. Gourlay日期:09/05/2019,浏览量:335,Q&A: 0
    Many Candida species, such as the opportunistic human pathogen Candida albicans, are Crabtree-Negative yeasts and are therefore highly dependent on the energy generated through oxidative phosphorylation. Respiration control is ...
    A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol
    一种广泛应用的尿素荧光/比色mrna原位杂交技术
    作者:Chiara Sinigaglia日期:09/05/2019,浏览量:726,Q&A: 0
    In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a ...
    Isolation of Neutrophil Nuclei for Use in NETosis Assays
    分离中性粒细胞核用于 NETosis分析
    作者:Dave Boucher日期:09/05/2019,浏览量:424,Q&A: 0
    Neutrophils are critical immune cells that protect our body against invading pathogens. They generate antibacterial DNA structures called neutrophil extracellular traps (NET). Recently we identified a new mechanism that enables NET formation. We ...
    Estrous Cycle Monitoring in Mice with Rapid Data Visualization and Analysis
    利用快速数据可视化分析进行小鼠发情周期监测
    作者:Leanna K. Pantier, Jiang Li and Catherine A. Christian日期:09/05/2019,浏览量:550,Q&A: 0
    The estrous cycle provides a readout of reproductive health in female laboratory rodents, and estrous cycle stage can be an important physiological variable. Accurate assessment of estrous cycle stage is also important in producing timed pregnancies ...
    In vitro Differentiation of Thymic Treg Cell Progenitors to Mature Thymic Treg Cells
    体外胸腺调节性T细胞祖细胞向成熟胸腺调节性T细胞的分化
    作者:David L. Owen and Michael A. Farrar日期:08/20/2019,浏览量:318,Q&A: 0
    Thymic Treg cell differentiation occurs via a two-step process. Step one generates Treg cell progenitors (TregP) and is driven by strong TCR interactions with antigens presented in the thymus. Step two is initiated ...
    Fibroblast Gap-closure Assay-Microscopy-based in vitro Assay Measuring the Migration of Murine Fibroblasts
    基于成纤维细胞间隙封闭显微成像的鼠成纤维细胞的体外迁移分析
    作者:Agnieszka P. Looney and Mallar Bhattacharya日期:08/20/2019,浏览量:519,Q&A: 0
    Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts.

    ...
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:306,Q&A: 0
    All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the ...
    Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay
    果蝇翅膀成虫盘体内培养和弗林蛋白酶抑制剂检测
    作者:Alex Sohr, Lijuan Du and Sougata Roy日期:08/20/2019,浏览量:353,Q&A: 0
    Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways. Classical genetic approaches and in vitro biochemical or ...
    A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
    利用改进的Sca/eS和机器学习用于螺旋器中毛细胞的成像和分析
    Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and ...
    Cell-based Assay for Recruitment of DDR1 to Collagen-coated Beads
    以细胞为基础的DDR1向胶原包被微珠的招募分析
    作者:Victoria Juskaite and Birgit Leitinger日期:08/20/2019,浏览量:352,Q&A: 0
    The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for ...
    Straight Channel Microfluidic Chips for the Study of Platelet Adhesion under Flow
    直通道微流控芯片用于研究流动中的血小板黏附
    作者:Alexander Dupuy, Lining Arnold Ju and Freda H Passam日期:03/20/2019,浏览量:2261,Q&A: 0
    Microfluidic devices have become an integral method of cardiovascular research as they enable the study of shear force in biological processes, such as platelet function and thrombus formation. Furthermore, microfluidic chips offer the benefits of ...
    Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
    整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
    作者:Rachna Narayanan and Andrew C. Oates日期:03/20/2019,浏览量:2520,Q&A: 0
    In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes ...
    Primary Embryonic Rat Cortical Neuronal Culture and Chronic Rotenone Treatment in Microfluidic Culture Devices
    原代胚胎大鼠皮质神经元培养并长期在微流控培养装置中鱼藤酮处理
    作者:Victor S. Van Laar, Beth Arnold and Sarah B Berman日期:03/20/2019,浏览量:1270,Q&A: 0
    In the study of neurodegenerative diseases, it is imperative to study the cellular and molecular changes associated with pathogenesis in the relevant cell type, central nervous system neurons. The unique compartmentalized morphology and bioenergetic ...
    Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes
    大鼠肾脏单纯线粒体的分离和线粒体呼吸链复合物蛋白质免疫印迹
    [Abstract] Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell cycle and metabolism. Thus, mitochondrial dysfunction is a hallmark of many chronic diseases ...
    Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices
    反义寡脱氧核苷酸灌注可阻突触可塑性相关蛋白在海马切片上的基因表达且无诱导补偿作用
    [Abstract] The elucidation of the molecular mechanisms of long-term synaptic plasticity has been hindered by both the compensation that can occur after chronic loss of the core plasticity molecules and by ex vivo conditions that may not reproduce in vivo plasticity. Here we describe a novel method to rapidly suppress gene expression by ...
    3D Organoid Formation from the Murine Salivary Gland Cell Line SIMS
    小鼠唾液腺SIMS细胞系的三维类器官形成
    作者:Harleen K. Athwal and Isabelle M. A. Lombaert日期:10/05/2019,浏览量:148,Q&A: 0
    [Abstract] Salivary glands consist of multiple phenotypically and functionally unique cell populations, such as the acinar, ductal, and myoepithelial cells that help produce, modify, and secrete saliva (Lombaert et al., 2011). Identification of mechanisms and factors that regulate these populations has been of key interest, as salivary gland-related ...
    Reconstituting Breast Tissue with Organotypic Three-dimensional Co-culture of Epithelial and Stromal Cells in Discontinuous Extracellular Matrices
    通过不连续细胞外基质中上皮细胞和基质细胞三维共培养重建乳腺组织
    作者:Gang Ren, Vandana Sharma, Joshua Letson, Yashna Walia, Veani Fernando and Saori Furuta日期:10/05/2019,浏览量:209,Q&A: 0
    [Abstract] Co-culture systems utilizing reconstituted or synthetic extracellular matrix (ECM) and micropatterning techniques have enabled the reconstruction of surface epithelial tissues. This technique has been utilized in the regeneration, disease modeling and drug screening of the surface epithelia, such as the skin and esophagus. On the other hand, the ...
    Measurement of Acid Ecto-phosphatase Activity in Live Leishmania donovani Parasites
    杜氏利什曼原虫中酸性胞外磷酸酶活性的活体检测
    作者:Amalia Papadaki and Haralabia Boleti日期:10/05/2019,浏览量:111,Q&A: 0
    [Abstract] Acid ecto-phosphatases are enzymes that hydrolyze phosphomonoesters in the acidic pH range with their active sites facing the extacellular medium. Their activities can be measured in living cells. In bacteria and protozoan pathogens, acid ecto-phosphatases have been associated with the survival of intracellular pathogens within phagocytes through ...
    Imaging the Vasculature of Immunodeficient Mice Using Positron Emission Tomography/Computed Tomography (PET/CT) and 18F-fluorodeoxyglucose Labeled Human Erythrocytes
    利用正电子发射断层扫描/计算机断层扫描(PET、CT)和18F-氟脱氧葡萄糖标记人类红细胞 进行免疫缺陷小鼠脉管系统成像
    作者:Shaowei Wang and Jung W. Choi日期:10/05/2019,浏览量:109,Q&A: 0
    [Abstract] Nuclear blood pool imaging using radiolabeled red blood cells has been used in the clinical setting for the evaluation of a number of medical conditions including gastrointestinal hemorrhage, impaired cardiac contractility, and altered cerebrovascular blood flow. Nuclear blood pool imaging is typically performed using Technetium-99m-labeled (99m ...
    A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-based Scaffolds (3D-dCBS)
    一种获得去细胞牛脊髓细胞外基质构成支架的新方法
    作者:Yavuz E. Arslan, Burcu Efe and Tugba Sezgin Arslan日期:10/05/2019,浏览量:145,Q&A: 0
    [Abstract] Extracellular matrix (ECM)-based tissue engineering scaffolds have an essential role in promoting tissue regeneration. Nerve tissue engineering aims at facilitating the repair of permanent damage to the peripheral and central nervous systems, which are difficult to heal. For this purpose, a variety of biomaterials are being developed consisting of ...
    Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal Mouse Macrophages by Flow Cytometry
    利用流式细胞术对巯基乙醇酸盐诱导的小鼠腹腔巨噬细胞中LPS-TLR4复合物内化的定量分析
    作者:Katharina Richard, Stefanie N. Vogel and Darren J. Perkins日期:09/20/2019,浏览量:207,Q&A: 0
    [Abstract] Internalization of cell surface Toll-like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using thioglycollate-elicited peritoneal macrophages by flow cytometry.
    Conjugation of Fab’ Fragments with Fluorescent Dyes for Single-molecule Tracking on Live Cells
    Fab片段结合荧光染料用于活细胞单分子示踪
    作者:I-Ting Teng, Xiangning Bu and Inhee Chung日期:09/20/2019,浏览量:241,Q&A: 0
    [Abstract] Our understanding of the regulation and functions of cell-surface proteins has progressed rapidly with the advent of advanced optical imaging techniques. In particular, single-molecule tracking (SMT) using bright fluorophores conjugated to antibodies and wide-field microscopy methods such as total internal reflection fluorescence microscopy have ...
    SIRF: A Single-cell Assay for in situ Protein Interaction with Nascent DNA Replication Forks
    SIRF:一种蛋白与新生DNA复制叉原位相互作用的单细胞检测办法
    作者:Sunetra Roy and Katharina Schlacher日期:09/20/2019,浏览量:300,Q&A: 0
    [Abstract] The duplication of DNA is a fundamental process that is required for the transfer of the genetic information from parent to daughter cells. Aberrant DNA replication processes are associated with diverse disease phenotypes, including developmental defects, ageing disorders, blood disorders such as Fanconi Anemia, increased inflammation and cancer. ...