细胞生物学

分类

    现刊
    In vitro Differentiation of Thymic Treg Cell Progenitors to Mature Thymic Treg Cells
    体外胸腺调节性T细胞祖细胞向成熟胸腺调节性T细胞的分化
    作者:David L. Owen and Michael A. Farrar日期:08/20/2019,浏览量:45,Q&A: 0
    Thymic Treg cell differentiation occurs via a two-step process. Step one generates Treg cell progenitors (TregP) and is driven by strong TCR interactions with antigens presented in the thymus. Step two is initiated ...
    Fibroblast Gap-closure Assay-Microscopy-based in vitro Assay Measuring the Migration of Murine Fibroblasts
    基于成纤维细胞间隙封闭显微成像的鼠成纤维细胞的体外迁移分析
    作者:Agnieszka P. Looney and Mallar Bhattacharya日期:08/20/2019,浏览量:67,Q&A: 0
    Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts.

    ...
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
    All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the ...
    Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay
    果蝇翅膀成虫盘体内培养和弗林蛋白酶抑制剂检测
    作者:Alex Sohr, Lijuan Du and Sougata Roy日期:08/20/2019,浏览量:34,Q&A: 0
    Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways. Classical genetic approaches and in vitro biochemical or ...
    A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
    利用改进的Sca/eS和机器学习用于螺旋器中毛细胞的成像和分析
    Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and ...
    Cell-based Assay for Recruitment of DDR1 to Collagen-coated Beads
    以细胞为基础的DDR1向胶原包被微珠的招募分析
    作者:Victoria Juskaite and Birgit Leitinger日期:08/20/2019,浏览量:43,Q&A: 0
    The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for ...
    Straight Channel Microfluidic Chips for the Study of Platelet Adhesion under Flow
    直通道微流控芯片用于研究流动中的血小板黏附
    作者:Alexander Dupuy, Lining Arnold Ju and Freda H Passam日期:03/20/2019,浏览量:1419,Q&A: 0
    Microfluidic devices have become an integral method of cardiovascular research as they enable the study of shear force in biological processes, such as platelet function and thrombus formation. Furthermore, microfluidic chips offer the benefits of ...
    Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
    整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
    作者:Rachna Narayanan and Andrew C. Oates日期:03/20/2019,浏览量:2012,Q&A: 0
    In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes ...
    Primary Embryonic Rat Cortical Neuronal Culture and Chronic Rotenone Treatment in Microfluidic Culture Devices
    原代胚胎大鼠皮质神经元培养并长期在微流控培养装置中鱼藤酮处理
    作者:Victor S. Van Laar, Beth Arnold and Sarah B Berman日期:03/20/2019,浏览量:1044,Q&A: 0
    In the study of neurodegenerative diseases, it is imperative to study the cellular and molecular changes associated with pathogenesis in the relevant cell type, central nervous system neurons. The unique compartmentalized morphology and bioenergetic ...
    In vitro Differentiation of Thymic Treg Cell Progenitors to Mature Thymic Treg Cells
    体外胸腺调节性T细胞祖细胞向成熟胸腺调节性T细胞的分化
    作者:David L. Owen and Michael A. Farrar日期:08/20/2019,浏览量:45,Q&A: 0
    [Abstract] Thymic Treg cell differentiation occurs via a two-step process. Step one generates Treg cell progenitors (TregP) and is driven by strong TCR interactions with antigens presented in the thymus. Step two is initiated by activation of STAT5 via IL-2, or IL-15, leading to mature Treg cells capable of ...
    Fibroblast Gap-closure Assay-Microscopy-based in vitro Assay Measuring the Migration of Murine Fibroblasts
    基于成纤维细胞间隙封闭显微成像的鼠成纤维细胞的体外迁移分析
    作者:Agnieszka P. Looney and Mallar Bhattacharya日期:08/20/2019,浏览量:67,Q&A: 0
    [Abstract] Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts.

    Currently, the two most widely used chemotaxis and cell migration assays are the scratch assay and the ...
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
    [Abstract] All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...
    Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay
    果蝇翅膀成虫盘体内培养和弗林蛋白酶抑制剂检测
    作者:Alex Sohr, Lijuan Du and Sougata Roy日期:08/20/2019,浏览量:34,Q&A: 0
    [Abstract] Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways. Classical genetic approaches and in vitro biochemical or cell biological assays identified that precursor forms of most growth factor family proteins are ...
    A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
    利用改进的Sca/eS和机器学习用于螺旋器中毛细胞的成像和分析
    [Abstract] Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and refractive index matching, which can all be completed within 72 h. Furthermore, we established automated ...
    Cell-based Assay for Recruitment of DDR1 to Collagen-coated Beads
    以细胞为基础的DDR1向胶原包被微珠的招募分析
    作者:Victoria Juskaite and Birgit Leitinger日期:08/20/2019,浏览量:43,Q&A: 0
    [Abstract] The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for in vitro studies. Thus, in vitro studies often use acid-soluble collagens in the ...
    Optimized Protocol for the Incorporation of FDAA (HADA Labeling) for in situ Labeling of Peptidoglycan
    FDAA用于肽聚糖原位标记的优选方案
    作者:Katharina Peters, Manuel Pazos, Michael S. VanNieuwenhze and Waldemar Vollmer日期:08/05/2019,浏览量:213,Q&A: 0
    [Abstract] The essential peptidoglycan (PG) layer surrounds the cytoplasmic membrane in nearly all bacteria. It is needed to maintain the shape of the cell and protect it from lysis due to high turgor. Growth of the PG layer is a complex process that involves the activities of PG synthases and hydrolases during elongation and cell division. PG growth sites ...
    Visualizing Hypoxia in a Murine Model of Candida albicans Infection Using in vivo Biofluorencence
    用活体生物荧光法观察小鼠白念珠菌感染模型中的缺氧情况
    作者:José Pedro Lopes and Constantin F. Urban日期:08/05/2019,浏览量:292,Q&A: 0
    [Abstract] Candida albicans is a leading human fungal pathogen that uses several metabolic adaptations to escape immune cells and causes systemic disease. Here, we describe a protocol for measuring one of these adaptations, the ability to thrive in hypoxic niches. Hypoxia was generated after successful subdermal infection with C. albicans ...
    Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
    利用 Fast Passive CLARITY技术研究斑马鱼、啮齿动物、灵长类动物和人类的大脑
    作者:Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova日期:08/05/2019,浏览量:249,Q&A: 0
    [Abstract] Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and stabilizing the cellular and subcellular structures. This provides effective penetration of diverse ...
    Image-based Quantification of Direct Cell-to-cell Transmission of Bovine Viral Diarrhea Virus
    基于成像量化分析牛病毒性腹泻病毒的细胞间传播
    作者:Fernando Merwaiss and Diego E. Alvarez日期:08/05/2019,浏览量:189,Q&A: 0
    [Abstract] Different viruses rely on direct cell-to-cell transmission to propagate infection within the infected host. Measuring this mode of transmission in cultured cells is often complicated by the contribution of cell free viruses to spread, and the difficulty to distinguish between primary infected cells that produce the virus and neighboring cells that ...