细胞生物学

分类

    现刊
    Preparation of Drosophila Polytene Chromosomes, Followed by Immunofluorescence Analysis of Chromatin Structure by Multi-fluorescence Correlations
    制备果蝇多线染色体并通过多重荧光关联对染色质结构进行免疫荧光分析
    作者:Terra M. Kuhn, Shawn C. Little and Maya Capelson日期:07/05/2020,浏览量:0,Q&A: 0
    Drosophila larval salivary gland polytene chromosome squashes have been used for decades to analyze genome-wide protein-binding patterns, transcriptional activation processes, and changes in chromatin structure at specific genetic loci. ...
    Observing Nutrient Gradients, Gene Expression and Growth Variation Using the "Yeast Machine" Microfluidic Device
    使用“酵母机”微流控设备观察营养梯度,基因表达和生长变异
    The natural environment of microbial cells like bacteria and yeast is often a complex community in which growth and internal organization reflect morphogenetic processes and interactions that are dependent on spatial position and time. While most of ...
    Microscopy-based Methods for Rosetting Assay in Malaria Research
    基于显微技术的疟疾玫瑰花环试验
    作者:Wenn-Chyau Lee and Laurent Rénia日期:07/05/2020,浏览量:0,Q&A: 0
    In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune ...
    Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis
    哺乳动物细胞脂筏的分离及其脂质组学分析
    作者:Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J. Meikle and Dmitri Sviridov日期:07/05/2020,浏览量:0,Q&A: 0
    Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways ...
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:0,Q&A: 0
    Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays ...
    Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli
    小鼠肾小球ICAM-1、ICAM-2和CD31的联合免疫染色
    作者:Sun-sang J. Sung日期:07/05/2020,浏览量:0,Q&A: 0
    Glomerulonephritis (GN) is a common pathological condition in chronic kidney diseases that often leads to end stage renal failure. Mac-1 (CD11b/CD18)-mediated neutrophil, macrophage, and dendritic cell glomerular infiltration leading to cellular ...
    In vivo Mouse Mammary Gland Formation
    小鼠体内乳腺形成
    作者:Heng Sun, Xin Zhang, Un In Chan, Sek Man Su, Sen Guo, Xiaoling Xu and Chuxia Deng日期:07/05/2020,浏览量:0,Q&A: 0
    For years, the mammary gland serves as a perfect example to study the self-renew and differentiation of adult stem cells, and the regulatory mechanisms of these processes as well. To assess the function of given genes and/or other factors on ...
    Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces
    小鼠粪便中短链脂肪酸的气相色谱检测
    作者:Chaozheng Zhang, An Liu, Tianshuang Zhang, Yi Li and Hua Zhao日期:07/05/2020,浏览量:15,Q&A: 0
    Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called ...
    Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres
    果蝇间接飞行肌肌节的超分辨显微镜观察
    作者:Szilárd Szikora, Tibor Novák, Tamás Gajdos, Miklós Erdélyi and József Mihály日期:06/20/2020,浏览量:451,Q&A: 0
    Sarcomeres are extremely highly ordered macromolecular assemblies where proper structural organization is an absolute prerequisite to the functionality of these contractile units. Despite the wealth of information collected, the exact spatial ...
    Maintenance and Quantitative Phenotyping of the Oomycete-plant Model Pathosystem Hyaloperonospora arabidopsidisArabidopsis
    卵菌-植物模型病理系统(活体营养型卵菌–拟南芥)的维持和定量表型分析
    作者:Fang-Yu Hwu and Martin Parniske日期:06/20/2020,浏览量:445,Q&A: 0
    The interaction between the host plant Arabidopsis thaliana (Arabidopsis) and the oomycete Hyaloperonospora arabidopsidis (Hpa) is an established model system for the study of an obligate biotrophic downy mildew ...
    Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
    神经退行性细胞模型中核质转运的定量分析
    作者:Joni Vanneste, Thomas Vercruysse, Philip Van Damme, Ludo Van Den Bosch and Dirk Daelemans日期:06/20/2020,浏览量:448,Q&A: 0
    Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and ...
    Quantification of Protein Kinase A (PKA) Activity by An in vitro Radioactive Assay Using the Mouse Sperm Derived Enzyme
    用小鼠精子衍生酶体外放射性测定蛋白激酶A(PKA)活性
    作者:Cintia Stival, Carolina Baro Graf, Pablo E. Visconti and Dario Krapf日期:06/20/2020,浏览量:563,Q&A: 0
    In order to acquire fertilizing potential, mammalian sperm must undergo a process known as capacitation, which relies on the early activation of Protein Kinase A (PKA). Frequently, PKA activity is assessed in whole-cell ...
    In vitro Crosslinking Reactions and Substrate Incorporation Assays for The Identification of Transglutaminase-2 Protein Substrates
    转谷氨酰胺酶-2蛋白底物的体外交联反应和底物掺入鉴定
    作者:William L. Willis, Abigail Foster, Caitlin Henry, Lai Chu Wu and Wael Jarjour日期:06/20/2020,浏览量:453,Q&A: 0
    Transglutaminase (TG2) catalyzes protein crosslinking between glutamyl and lysyl residues. Catalytic activity occurs via a transamidation mechanism resulting in the formation of isopeptide bonds. Since TG2-mediated transamidation is of mechanistic ...
    Preparation of HeLa Total Membranes and Assay of Lipid-inhibition of Serine Palmitoyltransferase Activity
    HeLa全膜的制备及其对丝氨酸-棕榈酰转移酶活性的抑制作用
    作者:Muthukumar Kannan, Deanna L. Davis, John Suemitsu, Christopher D. Oltorik and Binks Wattenberg日期:06/20/2020,浏览量:524,Q&A: 0
    Serine palmitoyltranferase (SPT) is a pyridoxal 5′ phosphate (PLP)-dependent enzyme that catalyzes the first and rate-limiting step of de novo synthesis of sphingolipids. SPT activity is homeostatically regulated in response to increased ...
    Single Cell Volume Measurement Utilizing the Fluorescence Exclusion Method (FXm)
    荧光排斥法(FXm)测量单细胞体积
    作者:Nash D. Rochman, Kai Yao, Nicolas A. Perez Gonzalez, Denis Wirtz and Sean X. Sun日期:06/20/2020,浏览量:602,Q&A: 0
    The measurement of single cell size remains an obstacle towards a deeper understanding of cell growth control, tissue homeostasis, organogenesis, and a wide range of pathologies. Recent advances have placed a spotlight on the importance of cell ...
    Live Cell Measurement of the Intracellular pH of Yeast by Flow Cytometry Using a Genetically-Encoded Fluorescent Reporter
    一种基于流式细胞术的利用基因编码荧光报告测定酵母活细胞内pH值的方法
    作者:Catherine G. Triandafillou and D. Allan Drummond日期:06/20/2020,浏览量:490,Q&A: 0
    The intracellular pH of yeast is a tightly regulated physiological cue that changes in response to growth state and environmental conditions. Fluorescent reporters, which have altered fluorescence in response to local pH changes, can be used to ...
    Isolation of Tumor Cells Based on Their Distance from Blood Vessels
    基于与血管距离的肿瘤细胞分离
    作者:Saran Kumar, Husni Sharife, Tirzah Kreisel, Libat Bar-Lev, Myriam Grunewald and Eli Keshet日期:05/20/2020,浏览量:787,Q&A: 0
    Differential exposure of tumor cells to microenvironmental cues greatly impacts cell phenotypes, raising a need for position based sorting of tumor cells amenable to multiple OMICs and functional analyses. One such key determinant of tumor ...
    Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice
    结核分枝杆菌感染小鼠肺和支气管肺泡灌洗样品中髓样细胞的流式细胞仪分析和荧光激活细胞分选
    作者:Alissa C. Rothchild, Dat Mai, Alan Aderem and Alan H. Diercks日期:05/20/2020,浏览量:844,Q&A: 0
    Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing ...
    Spatial Image Correlation Spectroscopy (ICS): A Technique for Average Size Determination of Subcellular Accumulated Structures from Fluorescence Microscopic Images
    空间图像相关光谱法(ICS):一种从荧光显微图像中确定亚细胞聚集结构平均尺寸的技术
    作者:Akira Kitamura and Masakata Kinjo日期:05/20/2020,浏览量:630,Q&A: 0
    Size determination of subcellular structures such as inclusion bodies (IBs) and granules from fluorescent images is important for identification and structural characterization. However, it is often time-consuming just for the comparison of the ...
    Preparation of Drosophila Polytene Chromosomes, Followed by Immunofluorescence Analysis of Chromatin Structure by Multi-fluorescence Correlations
    制备果蝇多线染色体并通过多重荧光关联对染色质结构进行免疫荧光分析
    作者:Terra M. Kuhn, Shawn C. Little and Maya Capelson日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] Drosophila larval salivary gland polytene chromosome squashes have been used for decades to analyze genome-wide protein-binding patterns, transcriptional activation processes, and changes in chromatin structure at specific genetic loci. There have been many evolutions of the squashing protocol over the years, with sub-optimal ...
    Observing Nutrient Gradients, Gene Expression and Growth Variation Using the "Yeast Machine" Microfluidic Device
    使用“酵母机”微流控设备观察营养梯度,基因表达和生长变异
    [Abstract] The natural environment of microbial cells like bacteria and yeast is often a complex community in which growth and internal organization reflect morphogenetic processes and interactions that are dependent on spatial position and time. While most of research is performed in simple homogeneous environments (e.g., bulk liquid cultures), ...
    Microscopy-based Methods for Rosetting Assay in Malaria Research
    基于显微技术的疟疾玫瑰花环试验
    作者:Wenn-Chyau Lee and Laurent Rénia日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune escape mechanism for the parasite. However, it has been associated with malaria pathogenesis, possibly ...
    Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis
    哺乳动物细胞脂筏的分离及其脂质组学分析
    作者:Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J. Meikle and Dmitri Sviridov日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and ...
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
    Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli
    小鼠肾小球ICAM-1、ICAM-2和CD31的联合免疫染色
    作者:Sun-sang J. Sung日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] Glomerulonephritis (GN) is a common pathological condition in chronic kidney diseases that often leads to end stage renal failure. Mac-1 (CD11b/CD18)-mediated neutrophil, macrophage, and dendritic cell glomerular infiltration leading to cellular dysfunction and destruction is an important disease mechanism. The cellular distribution and dynamics ...
    In vivo Mouse Mammary Gland Formation
    小鼠体内乳腺形成
    作者:Heng Sun, Xin Zhang, Un In Chan, Sek Man Su, Sen Guo, Xiaoling Xu and Chuxia Deng日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] For years, the mammary gland serves as a perfect example to study the self-renew and differentiation of adult stem cells, and the regulatory mechanisms of these processes as well. To assess the function of given genes and/or other factors on stemness of mammary cells, several In vitro assays were developed, such as mammospheres formation ...
    Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces
    小鼠粪便中短链脂肪酸的气相色谱检测
    作者:Chaozheng Zhang, An Liu, Tianshuang Zhang, Yi Li and Hua Zhao日期:07/05/2020,浏览量:15,Q&A: 0
    [Abstract] Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called short-chain fatty acids. SCFAs are closely related to various aspects of the human body, so more and ...
    Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres
    果蝇间接飞行肌肌节的超分辨显微镜观察
    作者:Szilárd Szikora, Tibor Novák, Tamás Gajdos, Miklós Erdélyi and József Mihály日期:06/20/2020,浏览量:451,Q&A: 0
    [Abstract] Sarcomeres are extremely highly ordered macromolecular assemblies where proper structural organization is an absolute prerequisite to the functionality of these contractile units. Despite the wealth of information collected, the exact spatial arrangement of many of the H-zone and Z-disk proteins remained unknown. Recently, we developed a powerful ...
    Maintenance and Quantitative Phenotyping of the Oomycete-plant Model Pathosystem Hyaloperonospora arabidopsidisArabidopsis
    卵菌-植物模型病理系统(活体营养型卵菌–拟南芥)的维持和定量表型分析
    作者:Fang-Yu Hwu and Martin Parniske日期:06/20/2020,浏览量:445,Q&A: 0
    [Abstract] The interaction between the host plant Arabidopsis thaliana (Arabidopsis) and the oomycete Hyaloperonospora arabidopsidis (Hpa) is an established model system for the study of an obligate biotrophic downy mildew interaction. The evaluation of the developmental success of Hpa is often based on the quantification ...