细胞生物学

分类

    现刊
    Characterization of Immunological Niches within Peyer’s Patches by ex vivo Photoactivation and Flow Cytometry Analysis
    体外光激活和流式细胞术分析派尔斑免疫龛位
    作者:Adi Biram and Ziv Shulman日期:03/20/2020,浏览量:191,Q&A: 0
    T follicular helper (Tfh) cells regulate B cell selection for entry into the germinal center (GC) reaction or for differentiation into antibody forming cells. This process takes place at the border between the T and B zones in lymphoid organs and ...
    An ex vivo Approach to Assess Mitochondrial ROS by Flow Cytometry in AAV-tagged Astrocytes in Adult Mice
    一种利用流式细胞术进行成年大鼠腺相关病毒标记星型胶质细胞中线粒体ROS水平的体外检测方法
    作者:Carlos Vicente-Gutierrez and Juan P. Bolaños日期:03/20/2020,浏览量:379,Q&A: 0
    Mitochondrial reactive oxygen species (mROS) are naturally produced signalling molecules extremely relevant for understanding both health- and disease-associated biological processes. The study of mROS in the brain is currently underway to decipher ...
    Analyzing the Quenchable Iron Pool in Murine Macrophages by Flow Cytometry
    小鼠巨噬细胞可淬灭铁池的流式细胞分析
    作者:Michael Riedelberger and Karl Kuchler日期:03/20/2020,浏览量:125,Q&A: 0
    Tissue-resident macrophages are pivotal for a tightly-regulated iron metabolism at a cellular and systemic level, since subtle iron alterations increase the susceptibility for microbial infections or drive multiple diseases. However, research on ...
    Method for Measuring Mucociliary Clearance and Cilia-generated Flow in Mice by ex vivo Imaging
    小鼠中黏膜纤毛清除和纤毛产生流动的离体成像检测方法
    作者:Eric Song and Akiko Iwasaki日期:03/20/2020,浏览量:178,Q&A: 0
    Ex vivo biophysical measurements provide valuable insights into understanding both physiological and pathogenic processes. One critical physiological mechanism that is regulated by these biophysical properties is cilia-generated flow that ...
    Optic Nerve Crush in Mice to Study Retinal Ganglion Cell Survival and Regeneration
    视神经挤压对小鼠视网膜神经节细胞存活和再生的影响
    作者:Evan G. Cameron, Xin Xia, Joana Galvao, Masoumeh Ashouri, Michael S. Kapiloff and Jeffrey L. Goldberg日期:03/20/2020,浏览量:168,Q&A: 0
    In diseases such as glaucoma, the failure of retinal ganglion cell (RGC) neurons to survive or regenerate their optic nerve axons underlies partial and, in some cases, complete vision loss. Optic nerve crush (ONC) serves as a useful model not only ...
    Testing Bone Formation Induction by Calvarial Injection Assay in vivo
    体内颅骨注射分析检测骨形成
    作者:Narelle E McGregor, Ingrid J Poulton, Emma C Walker and Natalie A Sims日期:03/20/2020,浏览量:256,Q&A: 0
    Bone formation occurs during embryogenesis, skeletal growth and during the process of skeletal renewal throughout life. In the process of bone formation, osteoblasts lay down a collagen-containing matrix, termed osteoid, which is gradually hardened ...
    Lipid Mixing Assay for Murine Myoblast Fusion and Other Slow Cell-cell Fusion Processes
    小鼠成肌细胞融合及其他慢细胞融合过程的脂质混合实验
    作者:Evgenia Leikina, Kamran Melikov, Anthony G. Rabinovich, Douglas P. Millay and Leonid V. Chernomordik日期:03/05/2020,浏览量:259,Q&A: 0
    Lipid mixing (redistribution of lipid probes between fusing membranes) has been widely used to study early stages of relatively fast viral and intracellular fusion processes that take seconds to minutes. Lipid mixing assays are especially important ...
    Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
    甜菜夜蛾对拟南芥的取食试验
    作者:Yanrong You, Chunpeng An and Chuanyou Li日期:03/05/2020,浏览量:261,Q&A: 0
    Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, ...
    Optogenetic Tuning of Ligand Binding to The Human T cell Receptor Using The opto-ligand-TCR System
    利用光配体TCR系统调节配体与人T细胞受体的结合
    T cells are one major cell type of the immune system that use their T cell antigen receptor (TCR) to bind and respond to foreign molecules derived from pathogens. The ligand-TCR interaction half-lives determine stimulation outcome. Until recently, ...
    Growth Recovery Assay and FACS-based Population Sorting Following Territorial Exclusion in Proteus mirabilis
    奇异变形杆菌被领土排斥后生长恢复测定和基于FACS的群体分类
    作者:Murray J. Tipping and Karine A. Gibbs日期:03/05/2020,浏览量:233,Q&A: 0
    Many bacteria take part in self recognition and kin discrimination behavior using contact-dependent effectors. Understanding the effects these effectors cause is important to explain bacterial community formation and population dynamics. Typically, ...
    Quantification of Protein Enrichment at Plasmodesmata
    胞间连丝富集蛋白的量化分析
    作者:Magali S. Grison, Jules D. Petit, Marie Glavier and Emmanuelle M. Bayer日期:03/05/2020,浏览量:315,Q&A: 0
    Intercellular communication plays a crucial role in the establishment of multicellular organisms by organizing and coordinating growth, development and defence responses. In plants, cell-to-cell communication takes place through nanometric membrane ...
    Tandem Tag Assay Optimized for Semi-automated in vivo Autophagic Activity Measurement in Arabidopsis thaliana roots
    优化的串联标签检测用于拟南芥根组织中体内半自动化自噬活性测定
    作者:Adrian N. Dauphinee, Jonas A. Ohlsson and Elena A. Minina日期:03/05/2020,浏览量:421,Q&A: 0
    Autophagy is the main catabolic process in eukaryotes and plays a key role in cell homeostasis. In vivo measurement of autophagic activity (flux) is a powerful tool for investigating the role of the pathway in organism development and ...
    Assembly of Genetic Circuits with the Mammalian ToolKit
    用哺乳动物工具包组装遗传电路
    作者:João P. Fonseca, Alain R Bonny, Jason Town and Hana El-Samad日期:03/05/2020,浏览量:393,Q&A: 0
    The ability to rapidly assemble and prototype cellular circuits is vital for biological research and its applications in biotechnology and medicine. The Mammalian ToolKit (MTK) is a Golden Gate-based cloning toolkit for fast, reproducible and ...
    Manganese Superoxide Dismutase Activity Assay in the Yeast Saccharomyces cerevisiae
    啤酒酵母中的锰超氧化物歧化酶活性测定
    作者:Louise Thines and Pierre Morsomme日期:03/05/2020,浏览量:387,Q&A: 0
    Superoxide dismutases (SODs) act as a primary defence against reactive oxygen species (ROS) by converting superoxide anion radicals (O2-) into molecular oxygen (O2) and hydrogen peroxide (H2O2). ...
    Cell-free Reconstitution of the Packaging of Cargo Proteins into Vesicles at the trans Golgi Network
    反面高尔基体网状结构中货物蛋白包装成囊泡的无细胞重组
    作者:Xiao Tang, Feng Yang and Yusong Guo日期:03/05/2020,浏览量:295,Q&A: 0
    Protein sorting at the trans Golgi network (TGN) plays important roles in targeting newly synthesized proteins to their specific destinations. The aim of this proposal is to reconstitute the packaging of non-Golgi resident cargo proteins ...
    Zebrafish Bacterial Infection Assay to Study Host-Pathogen Interactions
    斑马鱼细菌感染检测用于研究宿主-病原相互作用
    作者:Faiza Basheer, Clifford Liongue and Alister C Ward日期:03/05/2020,浏览量:257,Q&A: 0
    The study of host-pathogen interactions has improved our understanding of both pathogenesis and the response of the host to infection, including both innate and adaptive responses. Neutrophils and macrophages represent the first line of innate host ...
    Characterization of Immunological Niches within Peyer’s Patches by ex vivo Photoactivation and Flow Cytometry Analysis
    体外光激活和流式细胞术分析派尔斑免疫龛位
    作者:Adi Biram and Ziv Shulman日期:03/20/2020,浏览量:191,Q&A: 0
    [Abstract] T follicular helper (Tfh) cells regulate B cell selection for entry into the germinal center (GC) reaction or for differentiation into antibody forming cells. This process takes place at the border between the T and B zones in lymphoid organs and involves physical contacts between T and B cells. During these interactions, T cells endow the B cells ...
    An ex vivo Approach to Assess Mitochondrial ROS by Flow Cytometry in AAV-tagged Astrocytes in Adult Mice
    一种利用流式细胞术进行成年大鼠腺相关病毒标记星型胶质细胞中线粒体ROS水平的体外检测方法
    作者:Carlos Vicente-Gutierrez and Juan P. Bolaños日期:03/20/2020,浏览量:379,Q&A: 0
    [Abstract] Mitochondrial reactive oxygen species (mROS) are naturally produced signalling molecules extremely relevant for understanding both health- and disease-associated biological processes. The study of mROS in the brain is currently underway to decipher their physiopathological roles and contributions in neurological diseases. Recent advances in this ...
    Analyzing the Quenchable Iron Pool in Murine Macrophages by Flow Cytometry
    小鼠巨噬细胞可淬灭铁池的流式细胞分析
    作者:Michael Riedelberger and Karl Kuchler日期:03/20/2020,浏览量:125,Q&A: 0
    [Abstract] Tissue-resident macrophages are pivotal for a tightly-regulated iron metabolism at a cellular and systemic level, since subtle iron alterations increase the susceptibility for microbial infections or drive multiple diseases. However, research on cellular iron homeostasis in macrophages remains challenging due to the limited amount of available ...
    Method for Measuring Mucociliary Clearance and Cilia-generated Flow in Mice by ex vivo Imaging
    小鼠中黏膜纤毛清除和纤毛产生流动的离体成像检测方法
    作者:Eric Song and Akiko Iwasaki日期:03/20/2020,浏览量:178,Q&A: 0
    [Abstract] Ex vivo biophysical measurements provide valuable insights into understanding both physiological and pathogenic processes. One critical physiological mechanism that is regulated by these biophysical properties is cilia-generated flow that mediates mucociliary clearance, which is known to provide protection against foreign particles and ...
    Optic Nerve Crush in Mice to Study Retinal Ganglion Cell Survival and Regeneration
    视神经挤压对小鼠视网膜神经节细胞存活和再生的影响
    作者:Evan G. Cameron, Xin Xia, Joana Galvao, Masoumeh Ashouri, Michael S. Kapiloff and Jeffrey L. Goldberg日期:03/20/2020,浏览量:168,Q&A: 0
    [Abstract] In diseases such as glaucoma, the failure of retinal ganglion cell (RGC) neurons to survive or regenerate their optic nerve axons underlies partial and, in some cases, complete vision loss. Optic nerve crush (ONC) serves as a useful model not only of traumatic optic neuropathy but also of glaucomatous injury, as it similarly induces RGC cell death ...
    Testing Bone Formation Induction by Calvarial Injection Assay in vivo
    体内颅骨注射分析检测骨形成
    作者:Narelle E McGregor, Ingrid J Poulton, Emma C Walker and Natalie A Sims日期:03/20/2020,浏览量:256,Q&A: 0
    [Abstract] Bone formation occurs during embryogenesis, skeletal growth and during the process of skeletal renewal throughout life. In the process of bone formation, osteoblasts lay down a collagen-containing matrix, termed osteoid, which is gradually hardened by incorporation of mineral crystals. Although osteoblasts can be induced to differentiate and to ...
    Lipid Mixing Assay for Murine Myoblast Fusion and Other Slow Cell-cell Fusion Processes
    小鼠成肌细胞融合及其他慢细胞融合过程的脂质混合实验
    作者:Evgenia Leikina, Kamran Melikov, Anthony G. Rabinovich, Douglas P. Millay and Leonid V. Chernomordik日期:03/05/2020,浏览量:259,Q&A: 0
    [Abstract] Lipid mixing (redistribution of lipid probes between fusing membranes) has been widely used to study early stages of relatively fast viral and intracellular fusion processes that take seconds to minutes. Lipid mixing assays are especially important for identification of hemifusion intermediates operationally defined as lipid mixing without content ...
    Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
    甜菜夜蛾对拟南芥的取食试验
    作者:Yanrong You, Chunpeng An and Chuanyou Li日期:03/05/2020,浏览量:261,Q&A: 0
    [Abstract] Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...
    Optogenetic Tuning of Ligand Binding to The Human T cell Receptor Using The opto-ligand-TCR System
    利用光配体TCR系统调节配体与人T细胞受体的结合
    [Abstract] T cells are one major cell type of the immune system that use their T cell antigen receptor (TCR) to bind and respond to foreign molecules derived from pathogens. The ligand-TCR interaction half-lives determine stimulation outcome. Until recently, scientists relied on mutating either the TCR or its ligands to investigate how varying TCR-ligand ...
    Growth Recovery Assay and FACS-based Population Sorting Following Territorial Exclusion in Proteus mirabilis
    奇异变形杆菌被领土排斥后生长恢复测定和基于FACS的群体分类
    作者:Murray J. Tipping and Karine A. Gibbs日期:03/05/2020,浏览量:233,Q&A: 0
    [Abstract] Many bacteria take part in self recognition and kin discrimination behavior using contact-dependent effectors. Understanding the effects these effectors cause is important to explain bacterial community formation and population dynamics. Typically, kin discrimination effectors are toxins that kill target cells; their effect is therefore obvious ...