植物

分类

    现刊
    Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids
    从针叶树类囊体中分离光合巨复合体和超复合体的增溶方法
    作者:Pushan Bag, Wolfgang P. Schröder, Stefan Jansson and Domenica Farci日期:09/05/2021,浏览量:458,Q&A: 0

    Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades. In higher plants, the process takes place in the thylakoid

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    Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids
    从针叶树类囊体中分离光合巨复合体和超复合体的增溶方法
    作者:Pushan Bag, Wolfgang P. Schröder, Stefan Jansson and Domenica Farci日期:09/05/2021,浏览量:458,Q&A: 0
    [Abstract]

    Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades. In higher plants, the process takes place in the thylakoid membranes where the two photosystems (PSI and PSII) are located. In the past few decades, the evolution of

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    Tomato Stem Injection for the Precise Assessment of Ralstonia solanacearum Fitness in Planta
    番茄茎杆注射法精确评价青枯菌在植物中的适应性
    作者:Yaru Wang, Liu Xian, Gang Yu and Alberto P. Macho日期:08/20/2021,浏览量:384,Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a soil-borne pathogen with worldwide distribution that causes bacterial wilt disease in more than 250 plant species. R. solanacearum invades plants through the roots, reaches the vascular system, and colonizes the whole plant by moving through the xylem, where it eventually replicates rapidly, causing plant death. Usual

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    A Novel Method to Map Small RNAs with High Resolution
    一种新的高分辨率小RNA图谱绘制方法
    作者:Kun Huang, Feray Demirci, Blake C. Meyers and Jeffrey L. Caplan日期:08/20/2021,浏览量:558,Q&A: 0
    [Abstract]

    Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular molecule dynamics in situ. However, the application of super-resolution imaging techniques to detect

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    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    利用本生烟叶片基因瞬时表达或沉默研究青枯菌毒力的快速简便方法
    作者:Wenjia Yu and Alberto P. Macho日期:08/05/2021,浏览量:779,Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed by observation and scoring of disease symptoms. Here, we describe a novel inoculation protocol to

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    Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
    植物线粒体核糖体的纯化和低温电镜分析
    作者:Florent Waltz, Philippe Giegé and Yaser Hashem日期:08/05/2021,浏览量:767,Q&A: 0
    [Abstract]

    Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production. Among mitochondrial gene expression processes, translation is the least understood; although, recent advances

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    Spatiotemporal Quantification of Cytosolic pH in Arabidopsis Pollen Tubes
    拟南芥花粉管胞质pH的时空定量研究
    作者:Maria Teresa Portes, Daniel S.C. Damineli and José A. Feijó日期:07/20/2021,浏览量:809,Q&A: 0
    [Abstract]

    Ion-specific probes and fluorescent indicators have been key in establishing the role of ion signaling, namely calcium, protons, and anions, in plant development, providing a robust approach for monitoring spatiotemporal changes in intracellular ion dynamics. The integration of protons/pH in signaling mechanisms is especially important as reports

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    Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS
    气质联用技术分析水稻组织中微量可溶性糖含量
    作者:Li-he Xu, Li-ying Xiao, Xue-qiong Xiao, Yan-nong Xiao and Gao-feng Wang日期:07/05/2021,浏览量:1480,Q&A: 0
    [Abstract]

    Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies have been developed to quantitate soluble sugar content in plant tissues; however, it is difficult

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    Measurement of Transgene Copy Number in Plants Using Droplet Digital PCR
    应用微滴数字PCR技术测定植物中的转基因拷贝数
    作者:Yao-Min Cai, Quentin M. Dudley and Nicola J. Patron日期:07/05/2021,浏览量:1297,Q&A: 0
    [Abstract]

    Transgenic plants are produced both to investigate gene function and to confer desirable traits into crops. Transgene copy number is known to influence expression levels, and consequently, phenotypes. Similarly, knowledge of transgene zygosity is desirable for making quantitative assessments of phenotype and tracking the inheritance of transgenes

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    Peptide-mediated Targeting of Nanoparticles with Chemical Cargoes to Chloroplasts in Arabidopsis Plants
    多肽介导的化学物质纳米粒对拟南芥叶绿体的靶向作用
    作者:Israel Santana, Peiguang Hu, Su-Ji Jeon, Chris Castillo, Hann Tu and Juan Pablo Giraldo日期:06/20/2021,浏览量:1208,Q&A: 0
    [Abstract]

    Plant nanobiotechnology is a flourishing field that uses nanomaterials to study and engineer plant function. Applications of nanotechnology in plants have great potential as tools for improving crop yield, tolerance to disease and environmental stress, agrochemical delivery of pesticides and fertilizers, and genetic modification and transformation

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    Micrografting in Arabidopsis Using a Silicone Chip
    利用硅芯片进行拟南芥微嫁接
    作者:Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi日期:06/20/2021,浏览量:1591,Q&A: 1
    [Abstract]

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including defense mechanisms and responses to surrounding environments such as soil and light conditions.

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