蠕虫

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    现刊
    Cell Type-specific mRNA Purification in Caenorhabditis elegans via Translating Ribosome Affinity Purification
    秀丽隐杆线虫细胞特异性mRNA的核糖体亲和纯化
    作者:Ian G. McLachlan and Steven W. Flavell日期:08/05/2019,浏览量:261,Q&A: 0
    [Abstract] Cell type-specific molecular profiling is widely used to gain new insights into the diverse cell types that make up complex biological tissues. Translating ribosome affinity purification (TRAP) is a method in which the cell type-specific expression of epitope-tagged ribosomal subunits allows one to purify actively translating mRNAs without the ...
    Oxygen Consumption Measurements in Caenorhabditis elegans Using the Seahorse XF24
    利用海马细胞能量代谢实时测定仪XF24 进行秀丽隐杆线虫中耗氧量测定
    作者:Suraiya Haroon and Marc Vermulst日期:07/05/2019,浏览量:453,Q&A: 0
    [Abstract] Mitochondria generate 90% of the energy required to sustain life. As a result, loss of mitochondrial function compromises almost every facet of human physiology. Accordingly, most mitochondrial diseases tend to present themselves as complex, multi-systemic disorders that can be difficult to diagnose. Depending on the severity of the mitochondrial ...
    Measurement of Respiration Rate in Live Caenorhabditis elegans
    秀丽隐杆线虫活体呼吸速率的测定
    作者:Li Fang Ng and Jan Gruber日期:05/20/2019,浏览量:881,Q&A: 0
    [Abstract] Mitochondrial function and dysfunction are at the core of aging and involved in many age-dependent diseases. Rate of oxygen consumption is a measure of mitochondrial function and energy production rate. The nematode Caenorhabditis elegans (C. elegans) offers an opportunity to study “living” mitochondria without the need for ...
    Heterochronic Phenotype Analysis of Hypodermal Seam Cells in Caenorhabditis elegans
    秀丽隐杆线虫下皮干细胞的异时表型分析
    作者:Yon Ju Ji and Jiou Wang日期:01/05/2019,浏览量:1380,Q&A: 0
    [Abstract] Heterochrony refers to changes in the timing of developmental events, and it is precisely regulated in the organisms by the heterochronic genes such as C. elegans lin-4 and let-7. Mutations in these genes cause precocious or retarded development of certain cell lineages. With well-defined cell lineages, C. elegans ...
    Microirradiation for Precise, Double-strand Break Induction in vivo in Caenorhabditis elegans
    显微照射用于秀丽隐杆线虫中双链断裂的精确体外诱导
    作者:Kailey E. Harrell, Emily Koury and Sarit Smolikove日期:12/20/2018,浏览量:1348,Q&A: 0
    [Abstract] DNA double-strand breaks (DSBs) are toxic lesions that every cell must accurately repair in order to survive. The repair of DSBs is an integral part of a cell life cycle and can lead to lethality if repaired incorrectly. Laser microirradiation is an established technique which has been used in yeast, mammalian cell culture, and Drosophila ...
    Preparation of Silk Fibroin Nanoparticles and Enzyme-Entrapped Silk Fibroin Nanoparticles
    丝素蛋白纳米颗粒和酶包裹丝素蛋白纳米颗粒
    作者:Yu-Qing Zhang日期:12/20/2018,浏览量:1836,Q&A: 0
    [Abstract] After silk fiber is degummed in boiling 0.2% Na2CO3 solution, the degummed fibroin fiber could be dissolved in highly concentrated neutral salts such as CaCl2. The partially degraded polypeptides of silk fibroin, commonly called as regenerated liquid silk, could be obtained via water dialysis. The silk fibroin ...
    Blinded Visual Scoring of Images Using the Freely-available Software Blinder
    使用免费软件Blinder对图像进行盲视觉评分
    作者:Steven D. Cothren, Joel N. Meyer and Jessica H. Hartman日期:12/05/2018,浏览量:1314,Q&A: 0
    [Abstract] In nearly all subfields of biomedical sciences, there are phenotypes that are currently classified by expert visual scoring. In research applications, these classifications require the experimenter to be blinded to the treatment group in order to avoid unintentional bias in scoring. Currently, many labs either use laborious and tedious methods to ...
    Staining the Germline in Live Caenorhabditis elegans: Overcoming Challenges by Applying a Fluorescent-dye Feeding Strategy
    利用荧光染料喂食的方法活体染色标记秀丽隐杆线虫生殖腺
    作者:K. Adam Bohnert日期:11/05/2018,浏览量:1609,Q&A: 0
    [Abstract] C. elegans provides a tractable model organism for studying germline cell biology. Microscopy experiments are relatively facile, as this worm is transparent and germline development can be observed in real-time using DIC microscopy and/or fluorescent transgenes. Despite these many tools, robust staining techniques for imaging germ cells ...
    Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans
    秀丽隐杆线虫中细胞核/细胞质蛋白的分离
    作者:Alejandro Mata-Cabana, Olga Sin, Renée I. Seinstra and Ellen A. A. Nollen日期:10/20/2018,浏览量:2301,Q&A: 0
    [Abstract] C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans ...
    Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Caenorhabditis elegans Lysates
    使用秀丽隐杆线虫裂解液检测和定量聚谷氨酰胺聚集体的滤器延迟试验
    作者:Olga Sin, Alejandro Mata-Cabana, Renée I. Seinstra and Ellen A. A. Nollen日期:10/05/2018,浏览量:2283,Q&A: 0
    [Abstract] Protein aggregation is a hallmark of several neurodegenerative diseases and is associated with impaired protein homeostasis. This imbalance is caused by the loss of the protein’s native conformation, which ultimately results in its aggregation or abnormal localization within the cell. Using a C. elegans model of polyglutamine diseases, we ...