蠕虫

分类

    现刊
    Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays
    利用组蛋白肽阵列评估组蛋白修饰阅读蛋白的体外结合特异性
    作者:Mark W. Soo and Arneet L. Saltzman日期:09/20/2021,浏览量:45,Q&A: 0

    In the field of chromatin biology, a major goal of understanding the roles of histone post-translational modifications is to identify the proteins and domains that recognize these modifications. Synthetic histone peptides containing one or more

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    Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays
    利用组蛋白肽阵列评估组蛋白修饰阅读蛋白的体外结合特异性
    作者:Mark W. Soo and Arneet L. Saltzman日期:09/20/2021,浏览量:45,Q&A: 0
    [Abstract]

    In the field of chromatin biology, a major goal of understanding the roles of histone post-translational modifications is to identify the proteins and domains that recognize these modifications. Synthetic histone peptides containing one or more modifications are a key tool to probe these interactions in pull-down assays with recombinant proteins

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    Oil Red O Staining for Lipid Content in Caenorhabditis elegans
    油红O染色测定秀丽隐杆线虫脂质含量  
    作者:Feng-Yung Wang and Tsui-Ting Ching日期:08/20/2021,浏览量:848,Q&A: 0
    [Abstract]

    The nematode Caenorhabditis elegans has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has been validated as an accurate assessment for major fat storage in C. elegans. Here, we describe an

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    Ethanol-induced Sedative Behavior: An Assay to Investigate Increased Dopamine Signaling in Caenorhabditis elegans
    乙醇诱导的镇静行为:一项在秀丽隐杆线虫研究增加多巴胺信号
    作者:Anuradha Singh, Kavita Babu and Pratima Pandey日期:07/05/2021,浏览量:876,Q&A: 0
    [Abstract]

    Dopamine (DA) signaling affects locomotion, feeding, learning, and memory in C. elegans. Various assays have been developed to study the proteins involved in these behaviors; however, these assays show behavioral output only when there is a drastic change in DA levels. We designed an assay capable of observing behavioral output even with only

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    Analysis of Caenorhabditis elegans Sperm Number, Size, Activation, and Mitochondrial Content
    秀丽隐杆线虫精子数量、大小、活化及线粒体含量分析
    作者:Amy M. Hammerquist, Chia-An Yen and Sean P. Curran日期:06/05/2021,浏览量:1397,Q&A: 0
    [Abstract]

    Infertility is a widespread and often unexplained issue. Studying reproduction using C. elegans males offers insight into the influence of individual factors on male fertility in humans. We have created a collection of protocols to assess several aspects of C. elegans sperm quality, including number, size, rate of activation, and mitochondrial

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    Parasitemia Evaluation in Mice Infected with Schistosoma mansoni
    曼氏血吸虫感染小鼠的寄生虫病评估
    作者:Naiara Clemente Tavares and Marina Moraes Mourão日期:05/20/2021,浏览量:2150,Q&A: 0
    [Abstract]

    Schistosomiasis is a neglected tropical disease. Its treatment relies on the use of a single drug, praziquantel. Due to treatment limitations, an alternative for schistosomiasis chemotherapy is required; thus, a better understanding of parasite biology and host-parasite interactions is valuable to aid the identification of new anti-Schistosoma

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    Imaging and Fluorescence Quantification in Caenorhabditis elegans with Flow Vermimetry and Automated Microscopy
    秀丽隐杆线虫的流动蠕虫仪和自动显微镜成像和荧光定量研究
    作者:Elissa Tjahjono, Alexey V. Revtovich and Natalia V. Kirienko日期:05/20/2021,浏览量:1797,Q&A: 0
    [Abstract]

    Gene activation and cellular biomarkers are commonly monitored using fluorescent signals from transgenic reporters or dyes. These quantifiable markers are critical for biological research and serve as an incredibly powerful tool, even more so when combined with high-throughput screening. Caenorhabditis elegans is a particularly useful model in

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    A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy
    用常规和体积电子显微镜对秀丽隐杆线虫胚胎进行超微结构分析的高压冷冻和冷冻替代的工作流程
    作者:Mohammad M. Rahman, Irene Y. Chang, Orna Cohen-Fix and Kedar Narayan日期:04/05/2021,浏览量:3020,Q&A: 0
    [Abstract]

    The free-living nematode Caenorhabditis elegans is a popular model system for studying developmental biology. Here we describe a detailed protocol to high-pressure freeze the C. elegans embryo (either ex vivo after dissection, or within the intact worm) followed by quick freeze substitution. Processed samples are suitable for ultrastructural

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    Wounding Caenorhabditis elegans with Glass Wool
    玻璃棉用于秀丽隐杆线虫的创伤研究
    作者:Murugesan Pooranachithra, James Prabhanand Bhaskar and Krishnaswamy Balamurugan日期:01/20/2021,浏览量:1345,Q&A: 0
    [Abstract]

    Research on wound healing majorly relies on rat, mice and other animal models. However, an alternative animal model ought to be brought in the field, pertaining to the stringent ethical issues owing to the use of animals in research. In this regard, Caenorhabdits elegans, a miniature model nematode gains the great attention of the researchers in

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    Calcium Imaging of Neuronal Activity under Gradually Changing Odor Stimulation in Caenorhabditis elegans
    渐变气味刺激下秀丽隐杆线虫神经元活动的钙成像
    作者:Yuki Tanimoto and Koutarou D. Kimura日期:01/05/2021,浏览量:1400,Q&A: 0
    [Abstract]

    Olfactory behavior is among the most fundamental animal behaviors both in the wild and in the laboratory. To elucidate the neural mechanisms underlying olfactory behavior, it is critical to measure neural responses to odorant concentration changes resembling those that animals actually sense during olfactory behavior. However, reproducing the

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    Preparation of Nippostrongylus brasiliensis Larvae for the Study of Host Skin Response
    用于研究寄主皮肤反应的巴西日圆线虫幼虫的制备
    作者:Tiffany Bouchery, Gillian Coakley and Nicola L. Harris日期:12/20/2020,浏览量:923,Q&A: 0
    [Abstract]

    Hookworms are skin penetrating parasites, however in the laboratory the hookworm model Nippostrongylus brasiliensis, the parasite is traditionally administered subcutaneously bypassing the skin (epidermis and dermis). Here, we describe two complementary approaches for infecting mice with N. brasiliensis in order to study the skin immune responses.

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