蠕虫

分类

    现刊
    Bacterial Lawn Avoidance and Bacterial Two Choice Preference Assays in Caenorhabditis elegans
    秀丽隐杆线虫的细菌草坪趋避和细菌双选择参照实验
    作者:Jogender Singh and Alejandro Aballay日期:05/20/2020,浏览量:199,Q&A: 0
    Physical avoidance of pathogens is a crucial defense strategy used by the host to reduce pathogen infection. Hosts display the use of multiple strategies to sense and avoid pathogens, ranging from olfaction to sensing of damage caused by pathogen ...
    Bacterial Lawn Avoidance and Bacterial Two Choice Preference Assays in Caenorhabditis elegans
    秀丽隐杆线虫的细菌草坪趋避和细菌双选择参照实验
    作者:Jogender Singh and Alejandro Aballay日期:05/20/2020,浏览量:199,Q&A: 0
    [Abstract] Physical avoidance of pathogens is a crucial defense strategy used by the host to reduce pathogen infection. Hosts display the use of multiple strategies to sense and avoid pathogens, ranging from olfaction to sensing of damage caused by pathogen infection. Understanding various mechanisms of pathogen avoidance has the potential to uncover ...
    Quantification of Bacteria Residing in Caenorhabditis elegans Intestine
    秀丽隐杆线虫肠道细菌数量测定
    作者:M. Fernanda Palominos and Andrea Calixto日期:05/05/2020,浏览量:643,Q&A: 0
    [Abstract] Quantification of intestinal colonization by pathogenic or commensal bacteria constitute a critical part of the analysis to understand host-microbe interactions during different time points of their interplay. Here we detail a method to isolate non-pathogenic and pathogenic bacteria from C. elegans intestines, and classify gut phenotypes ...
    Auxin-mediated Protein Degradation in Caenorhabditis elegans
    生长素介导的秀丽隐杆线虫蛋白质降解
    作者:Michael A. Q. Martinez and David Q. Matus日期:04/20/2020,浏览量:691,Q&A: 0
    [Abstract] The auxin-inducible degron (AID) technology was recently adapted for use in the nematode Caenorhabditis elegans. Rapid degradation of C. elegans proteins tagged with an AID is mediated by a plant-specific F-box protein, transport inhibitor response 1 (TIR1), and occurs only in the presence of the phytohormone auxin. The first ...
    Isolation and Imaging of His- and RFP-tagged Amyloid-like Proteins from Caenorhabditis elegans by TEM and SIM
    TEM和SIM技术用于秀丽隐杆线虫His-和RFP标记淀粉样蛋白的分离和成像
    作者:Amberley D. Stephens, Meng Lu and Gabriele S. Kaminski Schierle日期:11/05/2019,浏览量:793,Q&A: 0
    [Abstract] In our recently published paper, we highlight that during normal aging of C. elegans age-dependent aggregates of proteins form and lead to functional decline of tissues. The protocol described here details the isolation of two proteins from C. elegans in their aggregated amyloid-like form, casein kinase I isoform alpha (KIN-19) ...
    Amplex Red Assay for Measuring Hydrogen Peroxide Production from Caenorhabditis elegans
    利用Amplex Red检测秀丽隐杆线虫中过氧化氢的生成
    作者:Ozgur Karakuzu, Melissa R. Cruz, Yi Liu and Danielle A. Garsin日期:11/05/2019,浏览量:1758,Q&A: 0
    [Abstract] Reagents such as Amplex® Red have been developed for detecting hydrogen peroxide (H2O2) and are used to measure the release of H2O2 from biological samples such as mammalian leukocytes undergoing the oxidative burst. Caenorhabditis elegans is commonly used as a model host in the study ...
    In situ Hybridization of Plant-parasitic Nematode Globodera pallida Juveniles to Detect Gene Expression
    原位杂交检测马铃薯白胞囊线虫的基因表达
    作者:Joanna Kud, Nejra Solo, Allan Caplan, Joseph C. Kuhl, Louise-Marie Dandurand and Fangming Xiao日期:09/20/2019,浏览量:1083,Q&A: 0
    [Abstract] In this study, we describe a standard whole mount in situ hybridization method which is used to determine the spatial-temporal expression pattern of genes from Globodera spp. Unlike more invasive radioactive labeling approaches, this technique is based on a safe, highly specific enzyme-linked immunoassay where a Digoxigenin ...
    Cell Type-specific mRNA Purification in Caenorhabditis elegans via Translating Ribosome Affinity Purification
    秀丽隐杆线虫细胞特异性mRNA的核糖体亲和纯化
    作者:Ian G. McLachlan and Steven W. Flavell日期:08/05/2019,浏览量:1305,Q&A: 0
    [Abstract] Cell type-specific molecular profiling is widely used to gain new insights into the diverse cell types that make up complex biological tissues. Translating ribosome affinity purification (TRAP) is a method in which the cell type-specific expression of epitope-tagged ribosomal subunits allows one to purify actively translating mRNAs without the ...
    Oxygen Consumption Measurements in Caenorhabditis elegans Using the Seahorse XF24
    利用海马细胞能量代谢实时测定仪XF24 进行秀丽隐杆线虫中耗氧量测定
    作者:Suraiya Haroon and Marc Vermulst日期:07/05/2019,浏览量:1747,Q&A: 0
    [Abstract] Mitochondria generate 90% of the energy required to sustain life. As a result, loss of mitochondrial function compromises almost every facet of human physiology. Accordingly, most mitochondrial diseases tend to present themselves as complex, multi-systemic disorders that can be difficult to diagnose. Depending on the severity of the mitochondrial ...
    Measurement of Respiration Rate in Live Caenorhabditis elegans
    秀丽隐杆线虫活体呼吸速率的测定
    作者:Li Fang Ng and Jan Gruber日期:05/20/2019,浏览量:2611,Q&A: 0
    [Abstract] Mitochondrial function and dysfunction are at the core of aging and involved in many age-dependent diseases. Rate of oxygen consumption is a measure of mitochondrial function and energy production rate. The nematode Caenorhabditis elegans (C. elegans) offers an opportunity to study “living” mitochondria without the need for ...
    Heterochronic Phenotype Analysis of Hypodermal Seam Cells in Caenorhabditis elegans
    秀丽隐杆线虫下皮干细胞的异时表型分析
    作者:Yon Ju Ji and Jiou Wang日期:01/05/2019,浏览量:2599,Q&A: 0
    [Abstract] Heterochrony refers to changes in the timing of developmental events, and it is precisely regulated in the organisms by the heterochronic genes such as C. elegans lin-4 and let-7. Mutations in these genes cause precocious or retarded development of certain cell lineages. With well-defined cell lineages, C. elegans ...