神经科学

分类

    现刊
    A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin
    一种高效生产和Src激酶介导的马海兔磷酸化的共纯化方法
    作者:Sherlene L. Brown, Yuan Ren, Daniel M. Suter and Seema Mattoo日期:09/20/2021,浏览量:286,Q&A: 0

    Cortactin is an actin-binding protein that regulates processes like cell migration, endocytosis, and tumor cell metastasis. Although cortactin is associated with actin-cytoskeletal dynamics in non-neuronal cells and cell-free systems, the exact

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    A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin
    一种高效生产和Src激酶介导的马海兔磷酸化的共纯化方法
    作者:Sherlene L. Brown, Yuan Ren, Daniel M. Suter and Seema Mattoo日期:09/20/2021,浏览量:286,Q&A: 0
    [Abstract]

    Cortactin is an actin-binding protein that regulates processes like cell migration, endocytosis, and tumor cell metastasis. Although cortactin is associated with actin-cytoskeletal dynamics in non-neuronal cells and cell-free systems, the exact mechanisms underlying its fundamental roles in neuronal growth cones are not fully explored. Recent

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    Measurement of LRRK2 Kinase Activity by Proximity Ligation Assay
    通过邻近连接测定法测量 LRRK2 激酶活性
    作者:Matthew T. Keeney, Eric K. Hoffman, J. Timothy Greenamyre and Roberto Di Maio日期:09/05/2021,浏览量:505,Q&A: 0
    [Abstract]

    Missense mutations in leucine rich-repeat kinase 2 (LRRK2) cause forms of familial Parkinson’s disease and have been linked to ‘idiopathic’ Parkinson’s disease. Assessment of LRRK2 kinase activity has been very challenging due to its size, complex structure, and relatively low abundance. A standard in the field to assess

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    Unraveling the Physicochemical Determinants of Protein Liquid-liquid Phase Separation by Nanoscale Infrared Vibrational Spectroscopy
    利用纳米尺度红外振动光谱揭示蛋白质液-液相分离的理化决定因素
    作者:Francesco S. Ruggeri, Alyssa M. Miller, Michele Vendruscolo and Tuomas P. J. Knowles日期:08/20/2021,浏览量:937,Q&A: 0
    [Abstract]

    The phenomenon of reversible liquid-liquid phase separation of proteins underlies the formation of membraneless organelles, which are crucial for cellular processes such as signalling and transport. In addition, it is also of great interest to uncover the mechanisms of further irreversible maturation of the functional dense liquid phase into

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    3D-printed Recoverable Microdrive and Base Plate System for Rodent Electrophysiology
    用于啮齿动物电生理学的3D打印可恢复微驱动器和基板系统
    [Abstract]

    Extracellular recordings in freely moving animals allow the monitoring of brain activity from populations of neurons at single-spike temporal resolution. While state-of-the-art electrophysiological recording devices have been developed in recent years (e.g., µLED and Neuropixels silicon probes), implantation methods for silicon probes in

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    Isolation of Nuclei from Mouse Dorsal Root Ganglia for Single-nucleus Genomics
    小鼠背根神经节细胞核的分离及其单核基因组学研究
    作者:Lite Yang, Ivan Tochitsky, Clifford J. Woolf and William Renthal日期:08/05/2021,浏览量:722,Q&A: 0
    [Abstract]

    Primary somatosensory neurons, whose cell bodies reside in the dorsal root ganglion (DRG) and trigeminal ganglion, are specialized to transmit sensory information from the periphery to the central nervous system. Our molecular understanding of peripheral sensory neurons has been limited by both their heterogeneity and low abundance compared with

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    Optical Clearing and 3D Analysis Optimized for Mouse and Human Pancreata
    小鼠和人胰腺的光学清除和三维分析优化
    [Abstract]

    The pancreas is a heavily innervated organ, but pancreatic innervation can be challenging to comprehensively assess using conventional histological methods. However, recent advances in whole-mount tissue clearing and 3D rendering techniques have allowed detailed reconstructions of pancreatic innervation. Optical clearing is used to enhance tissue

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    CRISPR-mediated Labeling of Cells in Chick Embryos Based on Selectively Expressed Genes
    基于选择性表达基因的CRISPR介导的鸡胚胎细胞标记
    作者:Masahito Yamagata and Joshua R. Sanes日期:08/05/2021,浏览量:1266,Q&A: 0
    [Abstract]

    The abilities to mark and manipulate specific cell types are essential for an increasing number of functional, structural, molecular, and developmental analyses in model organisms. In a few species, this can be accomplished by germline transgenesis; in other species, other methods are needed to selectively label somatic cells based on the genes

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    A Simple Spatial-independent Associative and Reversal Learning Task in Mice
    小鼠的一个简单的空间独立的联想和逆转学习任务
    作者:Fulvio Magara, Benjamin Boury-Jamot and Hanna Hörnberg日期:08/05/2021,浏览量:573,Q&A: 0
    [Abstract]

    The ability to adapt one's behavior in response to changing circumstances, or cognitive flexibility, is often altered in neuropsychiatric and neurodevelopmental conditions. In rodents, cognitive flexibility is frequently assessed using associative learning paradigms with a reversal component. The majority of existing protocols rely on

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    Cell-attached and Whole-cell Patch-clamp Recordings of Dopamine Neurons in the Substantia Nigra Pars Compacta of Mouse Brain Slices
    小鼠脑片黑质致密部多巴胺神经元的细胞贴附和全细胞膜片钳记录
    作者:Stefano Cattaneo, Maria Regoni, Jenny Sassone and Stefano Taverna日期:08/05/2021,浏览量:814,Q&A: 0
    [Abstract]

    The Substantia Nigra pars compacta (SNc) is a midbrain dopaminergic nucleus that plays a key role in modulating motor and cognitive functions. It is crucially involved in several disorders, particularly Parkinson’s disease, which is characterized by a progressive loss of SNc dopaminergic cells. Electrophysiological studies on SNc neurons are

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    Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts
    膜片钳移液管重复使用的快速酶清洗方法:通过消除膜片钳尝试之间的手动移液管更换来提高吞吐量
    作者:Corey R. Landry, Mighten C. Yip, Ilya Kolb, William A. Stoy, Mercedes M. Gonzalez and Craig R. Forest日期:07/20/2021,浏览量:899,Q&A: 0
    [Abstract]

    The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and automation were limited by the need to replace pipettes manually after each experiment. This

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