神经科学

分类

    现刊
    The Peak Interval Procedure in Rodents: A Tool for Studying the Neurobiological Basis of Interval Timing and Its Alterations in Models of Human Disease
    啮齿类动物的峰值间隔程序:研究人类疾病模型中间隔时间及其变化的神经生物学基础的工具
    作者:Fuat Balcı and David Freestone日期:09/05/2020,浏览量:351,Q&A: 0
    Animals keep track of time intervals in the seconds to minutes range with, on average, high accuracy but substantial trial-to-trial variability. The ability to detect the statistical signatures of such timing behavior is an indispensable feature of ...
    Identification of Socially-activated Neurons
    社交激活神经元的识别
    作者:Mary L. Phillips and Lucas Pozzo-Miller日期:09/05/2020,浏览量:471,Q&A: 0
    Determining the neuronal circuitry responsible for specific behaviors is a major focus in the field of neurobiology. Activity-dependent immediate early genes (IEGs), transcribed and translated shortly after neurons discharge action potentials, have ...
    Measuring Breathing Patterns in Mice Using Whole-body Plethysmography
    通过全身体积描记法测量小鼠的呼吸模式
    作者:Patricia Prada-Dacasa, Andrea Urpi, Laura Sánchez-Benito, Patrizia Bianchi and Albert Quintana日期:09/05/2020,浏览量:343,Q&A: 0
    Respiratory dysfunction is among the main cause of severe and fatal pathologies worldwide. The use of effective experimental models and methodologies for the study of the pulmonary pathophysiology is necessary to prevent, control and cure these ...
    Integration of Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons into Rat Brain
    人诱导多能干细胞(hiPSC)源神经元与大鼠脑回路的整合
    作者:Xiling Yin, Ted M. Dawson and Valina L. Dawson日期:09/05/2020,浏览量:639,Q&A: 0
    Human neuron transplantation offers novel opportunities for modeling human neurologic diseases and potentially replacement therapies. However, the complex structure of the human cerebral cortex, which is organized in six layers with tightly ...
    The Peak Interval Procedure in Rodents: A Tool for Studying the Neurobiological Basis of Interval Timing and Its Alterations in Models of Human Disease
    啮齿类动物的峰值间隔程序:研究人类疾病模型中间隔时间及其变化的神经生物学基础的工具
    作者:Fuat Balcı and David Freestone日期:09/05/2020,浏览量:351,Q&A: 0
    [Abstract] Animals keep track of time intervals in the seconds to minutes range with, on average, high accuracy but substantial trial-to-trial variability. The ability to detect the statistical signatures of such timing behavior is an indispensable feature of a good and theoretically-tractable testing procedure. A widely used interval timing procedure is the ...
    Identification of Socially-activated Neurons
    社交激活神经元的识别
    作者:Mary L. Phillips and Lucas Pozzo-Miller日期:09/05/2020,浏览量:471,Q&A: 0
    [Abstract] Determining the neuronal circuitry responsible for specific behaviors is a major focus in the field of neurobiology. Activity-dependent immediate early genes (IEGs), transcribed and translated shortly after neurons discharge action potentials, have been used extensively to either identify or gain genetic access to neurons and brain regions ...
    Measuring Breathing Patterns in Mice Using Whole-body Plethysmography
    通过全身体积描记法测量小鼠的呼吸模式
    作者:Patricia Prada-Dacasa, Andrea Urpi, Laura Sánchez-Benito, Patrizia Bianchi and Albert Quintana日期:09/05/2020,浏览量:343,Q&A: 0
    [Abstract] Respiratory dysfunction is among the main cause of severe and fatal pathologies worldwide. The use of effective experimental models and methodologies for the study of the pulmonary pathophysiology is necessary to prevent, control and cure these diseases. Plethysmography, a technique for the assessment of lung function, has been widely applied in ...
    Integration of Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons into Rat Brain
    人诱导多能干细胞(hiPSC)源神经元与大鼠脑回路的整合
    作者:Xiling Yin, Ted M. Dawson and Valina L. Dawson日期:09/05/2020,浏览量:639,Q&A: 0
    [Abstract] Human neuron transplantation offers novel opportunities for modeling human neurologic diseases and potentially replacement therapies. However, the complex structure of the human cerebral cortex, which is organized in six layers with tightly interconnected excitatory and inhibitory neuronal networks, presents significant challenges for in vivo ...
    Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUS
    核转运蛋白-β2抑制和ALS / FTD相关蛋白FUS的逆转聚集和液-液相分离
    作者:Emma Robinson, James Shorter and Lin Guo日期:08/20/2020,浏览量:758,Q&A: 0
    [Abstract] The study of RNA-binding proteins (RBP) offers insight into the mechanisms of pathologic protein aggregation in neurodegenerative diseases. We developed a protocol for purifying an RBP FUS and a nuclear import receptor (NIR) Kapβ2 and testing the ability of Kapβ2 to mitigate FUS aggregation and liquid-liquid phase separation.
    Assessing Gαq/15-signaling with IP-One: Single Plate Transfection and Assay Protocol for Cell-Based High-Throughput Assay
    使用IP-One评估Gαq/ 15信号:基于细胞高通量测定的单板转染和测定方案
    作者:Élie Besserer-Offroy, Rebecca L Brouillette, Jean-Michel Longpré and Philippe Sarret日期:08/20/2020,浏览量:632,Q&A: 0
    [Abstract] Cell-based functional assays are an important part of compound screening and drug lead optimization, and they can also play a crucial role in the determination of the residues involved in ligand binding and signaling for a particular G-protein-coupled receptor. Conventional methods used for Gαq/15-coupled receptors rely on the use of ...
    Sequential Reaching Task for the Study of Motor Skills in Monkeys
    猴子运动技能研究的连续性达标任务
    作者:Machiko Ohbayashi and Nathalie Picard日期:08/20/2020,浏览量:426,Q&A: 0
    [Abstract] The ability to perform a sequence of movements is a key component of motor skills, such as typing or playing a musical instrument. How the brain binds elementary movements together into meaningful actions has been a topic of much interest. Here, we describe two sequential reaching tasks that we use to investigate the neural substrate of skilled ...
    Co-culture of Murine Neurons Using a Microfluidic Device for The Study of Tau Misfolding Propagation
    利用微流体装置共培养小鼠神经元研究Tau的错误折叠传播
    作者:Grace I Hallinan, Dianne M Lopez, Mariana Vargas-Caballero, Jonathan West and Katrin Deinhardt日期:08/20/2020,浏览量:698,Q&A: 0
    [Abstract] The deposition of misfolded, aggregated tau protein is a hallmark of several neurodegenerative diseases, collectively termed “tauopathies”. Tau pathology spreads throughout the brain along connected pathways in a prion-like manner. The process of tau pathology propagation across circuits is a focus of intense research and has been investigated in ...
    A Reproducible Protocol to Measure the Critical Swimming Speed of Adult Zebrafish
    一种可重复性测定成年斑马鱼临界游泳速度的方法
    作者:Yuma Wakamatsu, Makoto Kashima and Hiromi Hirata日期:08/20/2020,浏览量:563,Q&A: 0
    [Abstract] The quantitative measurement of water flow-induced swimming of fish species using a swimmill is a powerful method to evaluate motor ability of individual fish. Zebrafish is a commonly used vertebrate that enables the study of morphological, physiological and behavioral characteristics associated with genes. We here established a reproducible ...
    Generation of Functional Mouse Hippocampal Neurons
    功能性小鼠海马神经元的产生
    作者:Francesco Tomassoni-Ardori, Zhenyi Hong, Gianluca Fulgenzi and Lino Tessarollo日期:08/05/2020,浏览量:860,Q&A: 0
    [Abstract] Primary culture of mouse hippocampal neurons is a very useful in vitro model for studying neuronal development, axonal and dendritic morphology, synaptic functions, and many other neuronal features. Here we describe a step-by-step process of generating primary neurons from mouse embryonic hippocampi (E17.5/E18.5). Hippocampal neurons ...