神经科学

分类

    现刊
    Construction of Viral Vectors for Cell Type-specific CRISPR Gene Editing in the Adult Mouse Brain
    用于成年小鼠脑组织的细胞特异性CRISPR基因编辑的病毒载体的构建
    作者:Hiroshi Yamaguchi and Luis de Lecea日期:08/20/2019,浏览量:71,Q&A: 0
    Recently developed gene editing technologies based on engineered CRISPR/Cas9 systems enables researchers to disrupt genes in a cell type-specific manner in the adult mouse brain. Using these technologies, we recently showed that the dopamine ...
    Cylinder Test to Assess Sensory-motor Function in a Mouse Model of Parkinson’s Disease
    基于圆筒试验的小鼠帕金森综合症模型中感觉运动系统功能测试
    Parkinson’s disease is a progressive neurodegenerative movement disorder that happens due to the loss of dopaminergic neurons in the substantia nigra. The deficiency of dopamine in the basal nuclei drives cardinal motor symptoms such as bradykinesia ...
    Whisker Nuisance Test: A Valuable Tool to Assess Tactile Hypersensitivity in Mice
    胡须损伤测试:一种检测小鼠触觉过敏症的有效方法
    作者:Luigi Balasco, Gabriele Chelini, Yuri Bozzi and Giovanni Provenzano日期:08/20/2019,浏览量:82,Q&A: 0
    Abnormal response to tactile stimulation, described as both hyper- and hypo-reactivity, is a common sensory impairment in multiple neuropsychiatric disorders. The neural bases of tactile sensitivity remain so far unknown. In the last years, animal ...
    Primary Embryonic Rat Cortical Neuronal Culture and Chronic Rotenone Treatment in Microfluidic Culture Devices
    原代胚胎大鼠皮质神经元培养并长期在微流控培养装置中鱼藤酮处理
    作者:Victor S. Van Laar, Beth Arnold and Sarah B Berman日期:03/20/2019,浏览量:1044,Q&A: 0
    In the study of neurodegenerative diseases, it is imperative to study the cellular and molecular changes associated with pathogenesis in the relevant cell type, central nervous system neurons. The unique compartmentalized morphology and bioenergetic ...
    Social Defeat Stress (SDS) in Mice: Using Swiss Mice as Resident
    Swiss小鼠作为研究对象研究小鼠的社会失败应激(SDS)
    作者:Marco Oreste F. Pagliusi Jr. and Cesar R. Sartori日期:03/20/2019,浏览量:1134,Q&A: 0
    Due to the high prevalence and great economic impact of depression, studies with animal models have been increasingly used to identify neurobiological mechanisms associated with this disorder. However, many animal models use stressful conditions ...
    Construction of Viral Vectors for Cell Type-specific CRISPR Gene Editing in the Adult Mouse Brain
    用于成年小鼠脑组织的细胞特异性CRISPR基因编辑的病毒载体的构建
    作者:Hiroshi Yamaguchi and Luis de Lecea日期:08/20/2019,浏览量:71,Q&A: 0
    [Abstract] Recently developed gene editing technologies based on engineered CRISPR/Cas9 systems enables researchers to disrupt genes in a cell type-specific manner in the adult mouse brain. Using these technologies, we recently showed that the dopamine beta-hydroxylase gene in Locus Coeruleus (LC) norepinephrine neurons plays a vital role in the maintenance ...
    Cylinder Test to Assess Sensory-motor Function in a Mouse Model of Parkinson’s Disease
    基于圆筒试验的小鼠帕金森综合症模型中感觉运动系统功能测试
    [Abstract] Parkinson’s disease is a progressive neurodegenerative movement disorder that happens due to the loss of dopaminergic neurons in the substantia nigra. The deficiency of dopamine in the basal nuclei drives cardinal motor symptoms such as bradykinesia and hypokinesia. The current protocol describes the cylinder test, which is a relatively simple ...
    Whisker Nuisance Test: A Valuable Tool to Assess Tactile Hypersensitivity in Mice
    胡须损伤测试:一种检测小鼠触觉过敏症的有效方法
    作者:Luigi Balasco, Gabriele Chelini, Yuri Bozzi and Giovanni Provenzano日期:08/20/2019,浏览量:82,Q&A: 0
    [Abstract] Abnormal response to tactile stimulation, described as both hyper- and hypo-reactivity, is a common sensory impairment in multiple neuropsychiatric disorders. The neural bases of tactile sensitivity remain so far unknown. In the last years, animal studies have proven to be useful for shedding light on the cellular and molecular mechanism ...
    Quantification of Prostaglandin E2 Concentration in Interstitial Fluid from the Hypothalamic Region of Free-moving Mice
    "自由活动小鼠下丘脑区域间质液中前列腺素E2浓度的定量分析
    作者:Yoshinobu Nakamura, Takatoshi Sakaguchi, Ikumi Tamai and Takeo Nakanishi日期:08/05/2019,浏览量:217,Q&A: 0
    [Abstract] Prostaglandin E2 (PGE2) is a well-established chemical mediator for the generation of the fever at the hypothalamus of the brain. PGE2 mediates fever generation via PGE receptor 3 (i.e., EP3) on neurons in the preoptic area. The role of PGE2 has been analyzed by measuring PGE2 ...
    Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
    利用 Fast Passive CLARITY技术研究斑马鱼、啮齿动物、灵长类动物和人类的大脑
    作者:Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova日期:08/05/2019,浏览量:249,Q&A: 0
    [Abstract] Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and stabilizing the cellular and subcellular structures. This provides effective penetration of diverse ...
    Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes
    神经元突起RNA含量的Axon-seq 深入分析
    作者:Jik Nijssen, Julio Aguila and Eva Hedlund日期:07/20/2019,浏览量:619,Q&A: 0
    [Abstract] Neuronal processes have an RNA composition that is distinct from the cell body. Therefore, to fully understand neuronal biology in health and disease we need to study both somas, dendrites and axons. Here we describe a detailed protocol of a newly refined method, Axon-seq, for RNA sequencing of axons (and dendrites) grown in isolation using single ...
    Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina
    等电聚焦用于小鼠视网膜中磷酸化视紫红质的定量分析
    作者:Sowmya Bekshe Lokappa, M. Carter Cornwall and Jeannie Chen日期:07/20/2019,浏览量:1154,Q&A: 0
    [Abstract] Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its ability to activate the visual G-protein transducin. Binding of light-activated, ...
    Protocol for Measuring Compulsive-like Feeding Behavior in Mice
    小鼠强迫性进食行为的检测步骤
    作者:Marta Fructuoso, Jose Espinosa-Carrasco, Ionas Erb, Cedric Notredame and Mara Dierssen日期:07/20/2019,浏览量:406,Q&A: 0
    [Abstract] Obesity is an important health problem with a strong environmental component that is acquiring pandemic proportion. The high availability of caloric dense foods promotes overeating potentially causing obesity. Animal models are key to validate novel therapeutic strategies, but researchers must carefully select the appropriate model to draw the ...
    A Standardized Tank Design for the Light Dark Task in Zebrafish
    一种用于探究斑马鱼明暗节律的培养箱标准设计
    作者:Amanda Facciol, Steven Tran and Robert Gerlai日期:07/20/2019,浏览量:297,Q&A: 0
    [Abstract] The light dark paradigm is a common behavioral test used to screen a variety of pharmacological agents, including anxiogenics and anxiolytics. Although most often used in rodents, the light dark task has recently been adapted for use in zebrafish. However, a number of inconsistent findings have been reported for this species. Some have found ...
    Total RNA Isolation from Separately Established Monolayer and Hydrogel Cultures of Human Glioblastoma Cell Line
    单层水凝胶培养人胶质母细胞瘤细胞系的全RNA分离
    作者:Manasi P Jogalekar and Elba E Serrano日期:07/20/2019,浏览量:659,Q&A: 0
    [Abstract] Astrocytoma is an invasive carcinoma occurring in the nervous system and currently lacks effective treatment options. A deeper understanding of the mechanisms of tumorigenesis and tumor progression is needed in order to develop novel therapeutic strategies. Recent advances in in vitro culture systems have demonstrated that the use of ...