神经科学

分类

    现刊
    Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices
    反义寡脱氧核苷酸灌注可阻突触可塑性相关蛋白在海马切片上的基因表达且无诱导补偿作用
    The elucidation of the molecular mechanisms of long-term synaptic plasticity has been hindered by both the compensation that can occur after chronic loss of the core plasticity molecules and by ex vivo conditions that may not reproduce in ...
    ATAC-seq on Sorted Adult Mouse Neurons
    ATAC-seq用于分选成熟小鼠神经元
    作者:Erin A. Clark, Yasuyuki Shima and Sacha Nelson日期:10/05/2019,浏览量:186,Q&A: 0
    Transcription regulation is a key aspect of cellular identity established during development and maintained into adulthood. Molecular and biochemical assays that probe the genome are critical tools in exploring mechanisms of transcription regulation ...
    Identification of Heteroreceptors Complexes and Signal Transduction Events Using Bioluminescence Resonance Energy Transfer (BRET)
    利用生物发光共振能量转移进行异受体复合物和信号转导的鉴定
    作者:Irene Reyes-Resina, Jasmina Jiménez, Gemma Navarro and Rafael Franco日期:10/05/2019,浏览量:142,Q&A: 0
    Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect ...
    An Operant Conditioning Task to Assess the Choice between Wheel Running and Palatable Food in Mice
    一项小鼠在转轮装置和可口食物之间选择的操作式条件反射测试
    作者:Bastien Redon, Imane Hurel, Giovanni Marsicano and Francis Chaouloff日期:10/05/2019,浏览量:219,Q&A: 0
    Wheel running, especially in the homecage, has been widely used to study the neurobiology of exercise because animal tends to use it voluntarily. However, as for each reward, its consumption (in the present case, running performance) does not ...
    Simple Protocol for Distinguishing Drug-induced Effects on Spatial Memory Acquisition, Consolidation and Retrieval in Mice Using the Morris Water Maze
    一种利用Morris水迷宫分辨药物诱导对于小鼠空间记忆获取、巩固和恢复学习行为影响的简单方案
    作者:Ariel K. Frame, Asad Lone, Richard A. Harris and Robert C. Cumming日期:09/20/2019,浏览量:206,Q&A: 0
    The Morris water maze (MWM) is one of the most commonly used tests for assessing spatial learning and memory in mice. While the MWM is highly amenable to testing the effects of memory modifying drugs, most studies do not consider the timing or ...
    Supported Cell Membrane Sheets to Monitor Protein Assembly
    蛋白质组装的细胞膜片监测
    作者:Simon Erlendsson, Thor Seneca Thorsen and Kenneth Lindegaard Madsen日期:09/20/2019,浏览量:251,Q&A: 0
    Studying protein-protein and protein-lipid interactions in their native environment is highly desirable, yet, the heterogeneity and complexity of cellular systems limits the repertoire of experimental methods available. In cells, interactions are ...
    Sciatic Nerve Cut and Repair Using Fibrin Glue in Adult Mice
    纤维蛋白胶在成年小鼠坐骨神经损伤修复中的应用
    作者:Erica T. Akhter, Travis M. Rotterman, Arthur W. English and Francisco J. Alvarez日期:09/20/2019,浏览量:211,Q&A: 0
    Peripheral nerve injury (PNI) is an excellent model for studying neural responses to injury and elucidating the mechanisms that can facilitate axon regeneration. As such, several animal models have been employed to study regenerative mechanisms ...
    Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation
    小鼠胚胎脊髓的组织块培养和体外电穿孔介导的基因转移
    Developing axons change responsiveness to guidance cues during the journey to synapse with target cells. Axon crossing at the ventral midline serves as a model for studying how axons accomplish such a switch in their response. Although primary ...
    A High-throughput and Pathophysiologically Relevant Astrocyte-motor Neuron Co-culture Assay for Amyotrophic Lateral Sclerosis Therapeutic Discovery
    高通量病理生理相关星形胶质细胞运动神经元共培养技术在肌萎缩侧索硬化治疗中的应用
    作者:Matthew J. Stopford, Scott P. Allen and Laura Ferraiuolo日期:09/05/2019,浏览量:621,Q&A: 0
    Amyotrophic lateral sclerosis (ALS) is an adult onset neurological disorder characterized by loss of motor neurons leading to progressive muscle wasting and eventually death. Astrocytes play a key role in disease pathogenesis. However, the ability ...
    Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells
    人干细胞源性神经元与少突胶质祖细胞的共培养
    作者:Stephanie Dooves, Aishwarya G. Nadadhur, Lisa Gasparotto and Vivi M. Heine日期:09/05/2019,浏览量:575,Q&A: 0
    Crosstalk between neurons and oligodendrocytes is important for proper brain functioning. Multiple co-culture methods have been developed to study oligodendrocyte maturation, myelination or the effect of oligodendrocytes on neurons. However, most of ...
    A Novel Dual Lentiviral CRISPR-based Transcriptional Activation System for Gene Expression Regulation in Neurons
    一种新型基于CAS9双载体慢病毒转录激活系统的神经组织基因表达调控
    作者:Katherine E. Savell, Faraz A. Sultan and Jeremy J. Day日期:09/05/2019,浏览量:708,Q&A: 0
    Robust and efficient gene expression control enables the study of a gene’s function in the central nervous system. Advances in CRISPR-based technology provide new avenues not only for gene editing, but for complex transcriptional control. Here, we ...
    Construction of Viral Vectors for Cell Type-specific CRISPR Gene Editing in the Adult Mouse Brain
    用于成年小鼠脑组织的细胞特异性CRISPR基因编辑的病毒载体的构建
    作者:Hiroshi Yamaguchi and Luis de Lecea日期:08/20/2019,浏览量:576,Q&A: 0
    Recently developed gene editing technologies based on engineered CRISPR/Cas9 systems enables researchers to disrupt genes in a cell type-specific manner in the adult mouse brain. Using these technologies, we recently showed that the dopamine ...
    Cylinder Test to Assess Sensory-motor Function in a Mouse Model of Parkinson’s Disease
    基于圆筒试验的小鼠帕金森综合症模型中感觉运动系统功能测试
    Parkinson’s disease is a progressive neurodegenerative movement disorder that happens due to the loss of dopaminergic neurons in the substantia nigra. The deficiency of dopamine in the basal nuclei drives cardinal motor symptoms such as bradykinesia ...
    Whisker Nuisance Test: A Valuable Tool to Assess Tactile Hypersensitivity in Mice
    胡须损伤测试:一种检测小鼠触觉过敏症的有效方法
    作者:Luigi Balasco, Gabriele Chelini, Yuri Bozzi and Giovanni Provenzano日期:08/20/2019,浏览量:381,Q&A: 0
    Abnormal response to tactile stimulation, described as both hyper- and hypo-reactivity, is a common sensory impairment in multiple neuropsychiatric disorders. The neural bases of tactile sensitivity remain so far unknown. In the last years, animal ...
    Primary Embryonic Rat Cortical Neuronal Culture and Chronic Rotenone Treatment in Microfluidic Culture Devices
    原代胚胎大鼠皮质神经元培养并长期在微流控培养装置中鱼藤酮处理
    作者:Victor S. Van Laar, Beth Arnold and Sarah B Berman日期:03/20/2019,浏览量:1270,Q&A: 0
    In the study of neurodegenerative diseases, it is imperative to study the cellular and molecular changes associated with pathogenesis in the relevant cell type, central nervous system neurons. The unique compartmentalized morphology and bioenergetic ...
    Social Defeat Stress (SDS) in Mice: Using Swiss Mice as Resident
    Swiss小鼠作为研究对象研究小鼠的社会失败应激(SDS)
    作者:Marco Oreste F. Pagliusi Jr. and Cesar R. Sartori日期:03/20/2019,浏览量:1334,Q&A: 0
    Due to the high prevalence and great economic impact of depression, studies with animal models have been increasingly used to identify neurobiological mechanisms associated with this disorder. However, many animal models use stressful conditions ...
    Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices
    反义寡脱氧核苷酸灌注可阻突触可塑性相关蛋白在海马切片上的基因表达且无诱导补偿作用
    [Abstract] The elucidation of the molecular mechanisms of long-term synaptic plasticity has been hindered by both the compensation that can occur after chronic loss of the core plasticity molecules and by ex vivo conditions that may not reproduce in vivo plasticity. Here we describe a novel method to rapidly suppress gene expression by ...
    ATAC-seq on Sorted Adult Mouse Neurons
    ATAC-seq用于分选成熟小鼠神经元
    作者:Erin A. Clark, Yasuyuki Shima and Sacha Nelson日期:10/05/2019,浏览量:186,Q&A: 0
    [Abstract] Transcription regulation is a key aspect of cellular identity established during development and maintained into adulthood. Molecular and biochemical assays that probe the genome are critical tools in exploring mechanisms of transcription regulation and cell type identity. The mammalian brain is composed of a huge diversity of cell types with ...
    Identification of Heteroreceptors Complexes and Signal Transduction Events Using Bioluminescence Resonance Energy Transfer (BRET)
    利用生物发光共振能量转移进行异受体复合物和信号转导的鉴定
    作者:Irene Reyes-Resina, Jasmina Jiménez, Gemma Navarro and Rafael Franco日期:10/05/2019,浏览量:142,Q&A: 0
    [Abstract] Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect interactions, but doubts arose due to specificity of the antibodies (monoclonal in the case of ...
    An Operant Conditioning Task to Assess the Choice between Wheel Running and Palatable Food in Mice
    一项小鼠在转轮装置和可口食物之间选择的操作式条件反射测试
    作者:Bastien Redon, Imane Hurel, Giovanni Marsicano and Francis Chaouloff日期:10/05/2019,浏览量:219,Q&A: 0
    [Abstract] Wheel running, especially in the homecage, has been widely used to study the neurobiology of exercise because animal tends to use it voluntarily. However, as for each reward, its consumption (in the present case, running performance) does not specifically provide information on its incentive value, i.e., the extent to which animals are ...
    Simple Protocol for Distinguishing Drug-induced Effects on Spatial Memory Acquisition, Consolidation and Retrieval in Mice Using the Morris Water Maze
    一种利用Morris水迷宫分辨药物诱导对于小鼠空间记忆获取、巩固和恢复学习行为影响的简单方案
    作者:Ariel K. Frame, Asad Lone, Richard A. Harris and Robert C. Cumming日期:09/20/2019,浏览量:206,Q&A: 0
    [Abstract] The Morris water maze (MWM) is one of the most commonly used tests for assessing spatial learning and memory in mice. While the MWM is highly amenable to testing the effects of memory modifying drugs, most studies do not consider the timing or duration of drug exposure when conducting the MWM assay; factors that can strongly influence the effect ...
    Supported Cell Membrane Sheets to Monitor Protein Assembly
    蛋白质组装的细胞膜片监测
    作者:Simon Erlendsson, Thor Seneca Thorsen and Kenneth Lindegaard Madsen日期:09/20/2019,浏览量:251,Q&A: 0
    [Abstract] Studying protein-protein and protein-lipid interactions in their native environment is highly desirable, yet, the heterogeneity and complexity of cellular systems limits the repertoire of experimental methods available. In cells, interactions are often taking place in confined microenvironments where factors such as avidity, hindered diffusion, ...
    Sciatic Nerve Cut and Repair Using Fibrin Glue in Adult Mice
    纤维蛋白胶在成年小鼠坐骨神经损伤修复中的应用
    作者:Erica T. Akhter, Travis M. Rotterman, Arthur W. English and Francisco J. Alvarez日期:09/20/2019,浏览量:211,Q&A: 0
    [Abstract] Peripheral nerve injury (PNI) is an excellent model for studying neural responses to injury and elucidating the mechanisms that can facilitate axon regeneration. As such, several animal models have been employed to study regenerative mechanisms after PNI, including Aplysia, zebrafish, rabbits, cats and rodents. This protocol describes how to ...
    Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation
    小鼠胚胎脊髓的组织块培养和体外电穿孔介导的基因转移
    [Abstract] Developing axons change responsiveness to guidance cues during the journey to synapse with target cells. Axon crossing at the ventral midline serves as a model for studying how axons accomplish such a switch in their response. Although primary neuron culture has been a versatile technique for elucidating various developmental mechanisms, many in ...
    A High-throughput and Pathophysiologically Relevant Astrocyte-motor Neuron Co-culture Assay for Amyotrophic Lateral Sclerosis Therapeutic Discovery
    高通量病理生理相关星形胶质细胞运动神经元共培养技术在肌萎缩侧索硬化治疗中的应用
    作者:Matthew J. Stopford, Scott P. Allen and Laura Ferraiuolo日期:09/05/2019,浏览量:621,Q&A: 0
    [Abstract] Amyotrophic lateral sclerosis (ALS) is an adult onset neurological disorder characterized by loss of motor neurons leading to progressive muscle wasting and eventually death. Astrocytes play a key role in disease pathogenesis. However, the ability to study astrocytic support towards motor neurons in ALS has been limited by a lack of sustainable ...
    Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells
    人干细胞源性神经元与少突胶质祖细胞的共培养
    作者:Stephanie Dooves, Aishwarya G. Nadadhur, Lisa Gasparotto and Vivi M. Heine日期:09/05/2019,浏览量:575,Q&A: 0
    [Abstract] Crosstalk between neurons and oligodendrocytes is important for proper brain functioning. Multiple co-culture methods have been developed to study oligodendrocyte maturation, myelination or the effect of oligodendrocytes on neurons. However, most of these methods contain cells derived from animal models. In the current protocol, we co-culture ...