神经科学

分类

    现刊
    Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease
    阿尔茨海默症幼鼠模型中海马单细胞记录和海马依赖先天性行为
    Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task ...
    Induction of Temporal Lobe Epilepsy in Mice with Pilocarpine
    毛果芸香碱诱导小鼠颞叶癫痫
    作者:Muhammad Nauman Arshad and Janice R Naegele日期:02/20/2020,浏览量:160,Q&A: 0
    In the pilocarpine model of temporal lobe epilepsy (TLE) in rodents, systemic injections of pilocarpine induce continuous, prolonged limbic seizures, a condition termed “Status Epilepticus” (SE). With appropriate doses, many inbred strains of mice ...
    Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus
    大鼠脉络丛原代上皮细胞培养方法
    作者:Valeria Lallai, Amina Ahmed and Christie D. Fowler日期:02/20/2020,浏览量:69,Q&A: 0
    The choroid plexus consists of a network of secretory epithelial cells localized throughout the lateral, third and fourth ventricles of the brain. Cerebrospinal fluid (CSF) is generated by the choroid plexus and released into the ventricular ...
    Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease
    阿尔茨海默症幼鼠模型中海马单细胞记录和海马依赖先天性行为
    [Abstract] Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of ...
    Induction of Temporal Lobe Epilepsy in Mice with Pilocarpine
    毛果芸香碱诱导小鼠颞叶癫痫
    作者:Muhammad Nauman Arshad and Janice R Naegele日期:02/20/2020,浏览量:160,Q&A: 0
    [Abstract] In the pilocarpine model of temporal lobe epilepsy (TLE) in rodents, systemic injections of pilocarpine induce continuous, prolonged limbic seizures, a condition termed “Status Epilepticus” (SE). With appropriate doses, many inbred strains of mice show behavioral seizures within an hour after pilocarpine is injected. With the behavioral scoring ...
    Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus
    大鼠脉络丛原代上皮细胞培养方法
    作者:Valeria Lallai, Amina Ahmed and Christie D. Fowler日期:02/20/2020,浏览量:69,Q&A: 0
    [Abstract] The choroid plexus consists of a network of secretory epithelial cells localized throughout the lateral, third and fourth ventricles of the brain. Cerebrospinal fluid (CSF) is generated by the choroid plexus and released into the ventricular environment. This biofluid contains an enriched source of proteins, ions, and other signaling molecules for ...
    Implanting and Recycling Neuropixels Probes for Recordings in Freely Moving Mice
    自由运动小鼠 Neuropixels 探针的植入与回收
    作者:Ashley L. Juavinett, George Bekheet and Anne K. Churchland日期:02/05/2020,浏览量:418,Q&A: 0
    [Abstract] Recording neural activity in unrestricted animals is necessary to unravel the neural basis of ethological behaviors. Recently, Neuropixels probes have made important strides in improving yield and lowering noise, but have limited use cases in freely moving animals. Although there are a number of studies demonstrating the use of these probes in ...
    A Simple and Efficient Method for Concomitant Isolation and Culture of Enriched Astroglial and Microglial Cells from the Rat Spinal Cord
    一种简单有效的大鼠脊髓星形胶质细胞和小胶质细胞联合分离培养方法
    作者:Pooja Shree Mishra and Trichur R Raju日期:01/20/2020,浏览量:540,Q&A: 0
    [Abstract] Investigations into glial biology have contributed substantially in understanding the physiology and pathology of the nervous system. However, intricacies of the neuron-glial and glial-glial interactions in vivo present significant challenges while delineating the individual cell-type contributions, thus making the in vitro ...
    Protocol for Measuring Free (Low-stress) Exploration in Rats
    大鼠自由(低应力)探索测量方案
    作者:Wojciech Pisula and Klaudia Modlinska日期:01/20/2020,浏览量:468,Q&A: 0
    [Abstract] Research on exploratory behavior plays a key part in behavioral science. Studying exploratory behavior of laboratory rodents may provide important data about many developmental and neurobiological processes occurring in animal ontogenesis. The proposed protocol for measuring the free (low-stress) exploration behavior in rats is straightforward, ...
    Studying Protein Aggregation in the Context of Liquid-liquid Phase Separation Using Fluorescence and Atomic Force Microscopy, Fluorescence and Turbidity Assays, and FRAP
    利用荧光和原子力显微镜、荧光和浊度分析以及FRAP研究液-液相分离中的蛋白质聚集
    作者:W. Michael Babinchak and Witold K. Surewicz日期:01/20/2020,浏览量:497,Q&A: 0
    [Abstract] Liquid-liquid phase separation (LLPS) underlies the physiological assembly of many membrane-less organelles throughout the cell. However, dysregulation of LLPS may mediate the formation of pathological aggregates associated with neurodegenerative diseases. Here, we present complementary experimental approaches to study protein aggregation within ...
    A Single Test to Study Social Behavior and Repetitive Self-grooming in Mice
    研究小鼠社交行为和重复自我修饰的单一试验
    作者:Niraj V. Lawande, Ammar L. Ujjainwala and Catherine A. Christian日期:01/20/2020,浏览量:401,Q&A: 0
    [Abstract] The ability to recognize and interact with members of the same species is essential for social communication. Investigating the neural substrates of social interest and recognition may offer insights into the behavioral differences present in disorders affecting social behavior. Assays used to study social interest in rodents include the 3-chamber ...
    Assessing Rough-and-tumble Play Behavior in Juvenile Rats
    幼年大鼠追逐打闹行为分析
    作者:Jonathan W. VanRyzin, Ashley E. Marquardt and Margaret M. McCarthy日期:01/05/2020,浏览量:509,Q&A: 0
    [Abstract] Play is a complex social behavior that is highly conserved across mammals. In most species, males engage in more frequent and vigorous play as juveniles than females, which reflects subtle yet impactful sex differences in brain circuitry and development. In this protocol, we describe a behavioral testing paradigm to assess social play in male and ...
    Laser Capture Micro-dissection (LCM) of Neonatal Mouse Forebrain for RNA Isolation
    新生小鼠前脑激光捕获显微切割(LCM)分离RNA
    作者:Achira Roy, Mei Deng, Kimberly A. Aldinger, Ian A. GLass and Kathleen J. Millen日期:01/05/2020,浏览量:466,Q&A: 0
    [Abstract] Precise and reproducible isolation of desired cell types or layers from heterogeneous tissues is crucial to analyze specific gene profiles and molecular interactions in vivo. Forebrain is the core site of higher functions, like cognition and memory consolidation. It is composed of heterogeneous and distinct cell types, interconnected to ...