节肢动物

分类

    现刊
    Generation, Analyzing and in-vivo Drug Treatment of Drosophila Models with IBMPFD
    果蝇IBMPFD模型的建立、分析及体内药物治疗
    作者:Ting Zhang, Bruce A. Hay and Ming Guo日期:05/20/2020,浏览量:217,Q&A: 0
    Missense mutations of p97/cdc48/Valosin-containing protein (VCP) cause inclusion body myopathy, Paget disease with frontotemporal dementia (IBMPFD) and other neurodegenerative diseases. The pathological mechanism of IBMPFD is not clear and ...
    Generation, Analyzing and in-vivo Drug Treatment of Drosophila Models with IBMPFD
    果蝇IBMPFD模型的建立、分析及体内药物治疗
    作者:Ting Zhang, Bruce A. Hay and Ming Guo日期:05/20/2020,浏览量:217,Q&A: 0
    [Abstract] Missense mutations of p97/cdc48/Valosin-containing protein (VCP) cause inclusion body myopathy, Paget disease with frontotemporal dementia (IBMPFD) and other neurodegenerative diseases. The pathological mechanism of IBMPFD is not clear and there is no treatment. We generated Drosophila models of IBMPFD in adult flight muscle in ...
    Application of Mechanical Forces on Drosophila Embryos by Manipulation of Microinjected Magnetic Particles
    机械力应用于果蝇胚胎的磁性粒子显微注射操作
    作者:Arturo D’Angelo and Jérôme Solon日期:05/05/2020,浏览量:438,Q&A: 0
    [Abstract] Cells generate mechanical forces to shape tissues during morphogenesis. These forces can activate several biochemical pathways and trigger diverse cellular responses by mechano-sensation, such as differentiation, division, migration and apoptosis. Assessing the mechano-responses of cells in living organisms requires tools to apply controlled local ...
    Bimolecular Fluorescence Complementation (BiFC) for Studying Sarcomeric Protein Interactions in Drosophila
    双分子荧光互补法研究果蝇肌节蛋白相互作用
    作者:Océane Marescal, Frieder Schöck and Nicanor González-Morales日期:04/05/2020,浏览量:354,Q&A: 0
    [Abstract] Protein-protein interactions in Drosophila myofibrils are essential for their function and formation. Bimolecular Fluorescence Complementation (BiFC) is an effective method for studying protein interactions and localization. BiFC relies on the reconstitution of a monomeric fluorescent protein from two half-fragments when in proximity. Two ...
    Phototactic T-maze Behavioral Assay for Comparing the Functionality of Color-sensitive Photoreceptor Subtypes in the Drosophila Visual System
    趋光T-迷宫行为分析用于果蝇视觉系统中色彩敏感光感应器亚型的比较
    作者:Hunter S. Shaw, Joe Larkin and Yong Rao日期:03/20/2020,浏览量:277,Q&A: 0
    [Abstract] The Drosophila retina contains light-sensitive photoreceptors (R cells) with distinct spectral sensitivities that allow them to distinguish light by its spectral composition. R7 and R8 photoreceptors are important for color vision, and can be further classified into pale (p) or yellow (y) subtypes depending on the rhodopsin expressed. ...
    Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
    甜菜夜蛾对拟南芥的取食试验
    作者:Yanrong You, Chunpeng An and Chuanyou Li日期:03/05/2020,浏览量:511,Q&A: 0
    [Abstract] Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...
    ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
    用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列
    作者:Junaid Akhtar, Piyush More and Steffen Albrecht日期:02/20/2020,浏览量:478,Q&A: 0
    [Abstract] Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as ...
    Preparation of Red Palm Weevil Rhynchophorus Ferrugineus (Olivier) (Coleoptera: Dryophthoridae) Germ-free Larvae for Host-gut Microbes Interaction Studies
    用于宿主肠道微生物相互作用研究的红棕象甲(稻铁甲虫)(鞘翅目:椰象鼻虫科)无菌幼虫的制备
    作者:Abrar Muhammad, Prosper Habineza, Youming Hou and Zhanghong Shi日期:12/20/2019,浏览量:886,Q&A: 0
    [Abstract] Red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, is a devastating pest of palm trees worldwide. RPW gut is colonized by diverse bacterial species which profoundly influence host development and nutritional metabolism. However, the molecular mechanisms behind the interactions between RPW and its gut microbiota remain mostly ...
    Measurement of Mitotic Spindle Angle and Mitotic Cell Distance in Fixed Tissue of Drosophila Larval Brains
    果蝇幼虫固定脑组织中有丝分裂纺锤体角度和有丝分裂细胞距离测定
    作者:Maribel Franco and Ana Carmena日期:11/20/2019,浏览量:702,Q&A: 0
    [Abstract] The positioning and the cleavage plane orientation of mitotic cells in pseudostratified epithelia (PSE) must be tightly regulated since failures in any of these processes might have fatal consequences during development. Here we present a simple method to determine the spindle orientation as well as the positioning of neuroepithelial mitotic cells ...
    Determination of Chromatin Accessibility in Drosophila Midgut Enterocytes by in situ 5mC Labeling
    利用原位5mC标记进行果蝇中肠细胞染色质开放性的鉴定
    作者:Eliya Bitman-Lotan, Hector Rincon-Arano, Gal Raz and Amir Orian日期:11/20/2019,浏览量:729,Q&A: 0
    [Abstract] Regulation of gene expression involves dynamic changes in chromatin organization, where in many cases open chromatin structure correlates with gene activation. Several methods enable monitoring changes in chromatin accessibility, including ATAC-seq, FAIRE-seq, MNase-seq and DNAse-seq methods, which involve Next-generation-sequencing (NGS). ...
    Characterization of Biological Motion Using Motion Sensing Superpixels
    基于运动传感超像素的生物运动特性研究
    作者:Felix Y. Zhou, Carlos Ruiz-Puig, Richard P. Owen, Michael J. White, Jens Rittscher and Xin Lu日期:09/20/2019,浏览量:1247,Q&A: 0
    [Abstract] Precise spatiotemporal regulation is the foundation for the healthy development and maintenance of living organisms. All cells must correctly execute their function in the right place at the right time. Cellular motion is thus an important dynamic readout of signaling in key disease-relevant molecular pathways. However despite the rapid ...