节肢动物

分类

    现刊
    Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay
    果蝇翅膀成虫盘体内培养和弗林蛋白酶抑制剂检测
    作者:Alex Sohr, Lijuan Du and Sougata Roy日期:08/20/2019,浏览量:34,Q&A: 0
    Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways. Classical genetic approaches and in vitro biochemical or ...
    Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay
    果蝇翅膀成虫盘体内培养和弗林蛋白酶抑制剂检测
    作者:Alex Sohr, Lijuan Du and Sougata Roy日期:08/20/2019,浏览量:34,Q&A: 0
    [Abstract] Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways. Classical genetic approaches and in vitro biochemical or cell biological assays identified that precursor forms of most growth factor family proteins are ...
    Optogenetic Inactivation of Transcription Factors in the Early Embryo of Drosophila
    果蝇早期胚胎中的光遗传失活转录因子
    作者:Stephen L. McDaniel and Melissa M. Harrison日期:07/05/2019,浏览量:591,Q&A: 0
    [Abstract] The early embryo of Drosophila melanogaster exists as a rapidly dividing syncytium of nuclei that are transcriptionally silent. Maternally deposited factors are required to awaken the genome and assist in the transition from maternal to zygotic control of development. Because many of these essential factors are maternally deposited and ...
    Measurement of Sleep and Arousal in Drosophila
    果蝇睡眠和觉醒的检测
    作者:Margaret E. Driscoll, Callen Hyland and Divya Sitaraman日期:06/20/2019,浏览量:554,Q&A: 0
    [Abstract] Sleep is a conserved neurobehavioral state observed in animals with sufficiently complex nervous systems and is critical for survival. While the exact function of sleep remains unknown, the lack of sleep can have a range of physiological and behavioral effects. Studies in invertebrates and vertebrates have identified conserved neural mechanisms ...
    Accessing Olfactory Habituation in Drosophila melanogaster with a T-maze Paradigm
    使用T迷宫范例模拟黑腹果蝇的嗅觉习性
    作者:Ourania Semelidou, Summer F. Acevedo and Efthimios M.C. Skoulakis日期:06/05/2019,浏览量:613,Q&A: 0
    [Abstract] Habituation is the process whereby perceptual changes alter the value of environmental stimuli, enabling salience filtering. This behavioral response decrement is a form of non-associative learning, where the subject learns about the stimulus and does not involve sensory adaptation, sensory or motor fatigue. The range of behavioral responses in D. ...
    Hypochlorous Acid Staining with R19-S in the Drosophila Intestine upon Ingestion of Opportunistic Bacteria
    果蝇肠道摄入外来细菌后次氯酸R19-S染色
    作者:Salma Hachfi, Olivia Benguettat and Armel Gallet日期:05/20/2019,浏览量:645,Q&A: 0
    [Abstract] The intestine is endowed with an innate immune system that is required to fight any exogenous bacteria that are swallowed along with the food. The first line of defense that is mounted by the gut epithelium is the release of immune Reactive Oxygen Species (ROS), such as hypochlorous acid (HOCl), into the lumen. HOCl is produced within 1.5 h of ...
    pNP Transgenic RNAi System Manual in Drosophila
    果蝇中pNP RNAi 转基因系统手册
    作者:Fang Wang, Huan-Huan Qiao, Rong-Gang Xu, Jin Sun, Ruibao Zhu, Decai Mao and Jian-Quan Ni 日期:02/05/2019,浏览量:1137,Q&A: 0
    [Abstract] Much of our knowledge about the mechanisms underlying biological processes relies on genetic approaches, whereby gene activity is reduced and the phenotypic consequences of perturbation are analyzed in detail. For functional genomic studies, a specific, systematic, and cost-effective manner is critical. Transgenic RNAi system is the top priority ...
    Flip-flop Mediated Conditional Gene Inactivation in Drosophila
    果蝇中Flip-flop介导的条件性基因失活
    作者:Sathiya N. Manivannan, Priyanka Pandey and Sonal Nagarkar-Jaiswal日期:02/05/2019,浏览量:1376,Q&A: 0
    [Abstract] Mosaic analysis in Drosophila, an important tool to assess cellular phenotypes of mutants in an otherwise heterozygous background, relies on mitosis. Hence, it cannot be used to inactivate gene function in mitotically inactive, terminally differentiated cells such as neurons. To address this issue, we developed “Flip-flop”, a novel, ...
    A Novel Hygrotaxis Assay for Assessing Thirst Perception and Water Sensation in Drosophila
    一种测定果蝇口渴感知和水感应的新型趋湿性测定法
    作者:Yuanjie Sun, Shan Gao, Feiteng Ji and Yan Zhu日期:02/05/2019,浏览量:943,Q&A: 0
    [Abstract] Water is essential for the survival of terrestrial animals. Animals obtain water from their food, from metabolism and, more importantly, by drinking. It is therefore vital for dehydrated animals to efficiently locate water sources in the wild. Traditional paradigm for studying the genetic basis of humidity sensation and preference use an apparatus ...
    Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila
    果蝇中通过“链接”化学检测标记的从头合成蛋白
    [Abstract] Copper-catalyzed azide-alkyne-cycloaddition (CuAAC), also known as ‘click chemistry’ serves as a technique for bio-orthogonal, that is, bio-compatible labeling of macromolecules including proteins or lipids. Click chemistry has been widely used to covalently, selectively, and efficiently attach probes such as fluorophores or biotin to small ...
    flySAM Transgenic CRISPRa System Manual
    基于flySAM的CRISPRa转基因系统操作手册
    作者:Yu Jia, Da Shen, Xia Wang, Jin Sun, Ping Peng, Rong-Gang Xu, Bowen Xu and Jian-Quan Ni 日期:01/20/2019,浏览量:1723,Q&A: 0
    [Abstract] Powerful and general methods that can enhance gene expression are useful to systematically study gene function. To date, compared with the methods in generating loss-of-function mutants, methods to achieve gain-of-function are limited. The entire field in Drosophila has relied heavily on the Gal4/UAS:cDNA overexpression system developed ...