鱼类

分类

    现刊
    Quantitative Determination of Ca2+-binding to Ca2+-sensor Proteins by Isothermal Titration Calorimetry
    钙离子与钙离子传感器蛋白结合的等温滴定量热法定量测定
    作者:Seher Abbas and Karl-Wilhelm Koch日期:04/05/2020,浏览量:385,Q&A: 0
    [Abstract] Diverse and complex molecular recognitions are central elements of signal transduction cascades. The strength and nature of these interaction modes can be determined by different experimental approaches. Among those, Isothermal titration calorimetry (ITC) offers certain advantages by providing binding constants and thermodynamic parameters from ...
    mRNA Extraction from Gill Tissue for RNA-sequencing
    鳃组织mRNA 提取用于RNA测序
    作者:Jukka-Pekka Verta and Felicity Jones日期:03/05/2020,浏览量:489,Q&A: 0
    [Abstract] Adaptation is thought to proceed in part through spatial and temporal changes in gene expression. Fish species such as the threespine stickleback are powerful vertebrate models to study the genetic architecture of adaptive changes in gene expression since divergent adaptation to different environments is common, they are abundant and easy to study ...
    Zebrafish Bacterial Infection Assay to Study Host-Pathogen Interactions
    斑马鱼细菌感染检测用于研究宿主-病原相互作用
    作者:Faiza Basheer, Clifford Liongue and Alister C Ward日期:03/05/2020,浏览量:506,Q&A: 0
    [Abstract] The study of host-pathogen interactions has improved our understanding of both pathogenesis and the response of the host to infection, including both innate and adaptive responses. Neutrophils and macrophages represent the first line of innate host defense against any infection. The zebrafish is an ideal model to study the response of these cells ...
    Retina Injury and Retina Tissue Preparation to Study Regeneration in Zebrafish
    通过斑马鱼视网膜损伤和视网膜组织制备进行视力恢复研究
    作者:Poonam Sharma and Rajesh Ramachandran日期:12/20/2019,浏览量:960,Q&A: 0
    [Abstract] Unlike mammals, primitive vertebrates have immense capability to regenerate almost all of their organs including the central nervous system. Among primitive organisms, zebrafish have been extensively used as a model system for regeneration studies. The retina is a part of the central nervous system and mammals lack the potential to repair any ...
    Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
    利用 Fast Passive CLARITY技术研究斑马鱼、啮齿动物、灵长类动物和人类的大脑
    作者:Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova日期:08/05/2019,浏览量:2391,Q&A: 0
    [Abstract] Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and stabilizing the cellular and subcellular structures. This provides effective penetration of diverse ...
    A Standardized Tank Design for the Light Dark Task in Zebrafish
    一种用于探究斑马鱼明暗节律的培养箱标准设计
    作者:Amanda Facciol, Steven Tran and Robert Gerlai日期:07/20/2019,浏览量:1494,Q&A: 0
    [Abstract] The light dark paradigm is a common behavioral test used to screen a variety of pharmacological agents, including anxiogenics and anxiolytics. Although most often used in rodents, the light dark task has recently been adapted for use in zebrafish. However, a number of inconsistent findings have been reported for this species. Some have found ...
    Quantification of Cutaneous Ionocytes in Small Aquatic Organisms
    小型水生生物皮肤离子细胞的定量分析
    作者:Garfield T. Kwan, Shane H. Finnerty, Nicholas C. Wegner and Martin Tresguerres日期:05/05/2019,浏览量:1499,Q&A: 0
    [Abstract] Aquatic organisms have specialized cells called ionocytes that regulate the ionic composition, osmolarity, and acid/base status of internal fluids. In small aquatic organisms such as fishes in their early life stages, ionocytes are typically found on the cutaneous surface and their abundance can change to help cope with various metabolic and ...
    Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
    整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
    作者:Rachna Narayanan and Andrew C. Oates日期:03/20/2019,浏览量:5108,Q&A: 0
    [Abstract] In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non-radioactive labels that can be detected by immunohistochemistry and coupled to ...
    Notochord Injury Assays that Stimulate Transcriptional Responses in Zebrafish Larvae
    诱导幼年期斑马鱼转录响应的脊索损伤实验
    [Abstract] Zebrafish have become an increasingly important model organism in the field of wound healing and regenerative medicine, due to their high regenerative capacity coupled with high-resolution imaging in living animals. In a recent study, we described multiple physical and chemical methods to induce notochord injury that led to highly specific ...
    Mosaic Labeling and 3-Dimensional Morphological Analysis of Single Cells in the Zebrafish Left-right Organizer
    斑马鱼左右组织者中单细胞的嵌合标记和3D形态分析
    作者:Agnik Dasgupta, Andrew E. Jacob and Jeffrey D. Amack日期:11/20/2018,浏览量:2377,Q&A: 0
    [Abstract] A transient epithelial structure called the left-right organizer (LRO) establishes left-right asymmetry in vertebrate embryos. Developmental defects that alter LRO formation result in left-right patterning errors that often lead to congenital heart malformations. However, little is known about mechanisms that regulate individual cell behaviors ...