联合作者(
Vanesa Olivares-Illana Instituto de Física, Universidad Autónoma de San Luis Potosí
2 protocols

Robin Farhaeus
  • Cibles Therapeutiques, INSERM Unite 940, Universite Paris 7, France
研究方向
  • 生物化学
  • 已发表 protocol 2
已发表 protocol 2篇
Purification of His-ubiquitin Proteins from Mammalian Cells
从哺乳动物细胞中纯化带His标签泛素化蛋白

作者:Vanesa Olivares Illana and Robin Farhaeus日期:09/05/2012,浏览量:19911,Q&A: 10
This protocol is used to purify His-tag ubiquitin conjugated protein. In this particular case, cells were transfected with His-tag ubiquitin and p53 which allowed us to purify using His-tag and reveal the WB using antibodies against p53 to see just ...
Protein-RNA ELISA Assay
蛋白质-RNA酶联免疫吸附试验

作者:Vanesa Olivares Illana and Robin Farhaeus日期:09/05/2012,浏览量:12108,Q&A: 0
Protein-RNA ELISA assay is an effective and quantitative method to study protein-RNA interactions in vitro. In this protocol we used recombinant 6x HIS tagged protein, but it works as well for non tagged proteins.