联合作者(
John Thomas Beatty Department of Microbiology and Immunology, University of British Columbia, Vancouver
5 protocols
联合评审(
Indranil Malik University of Michigan
9 protocols

Renate Weizbauer Carnegie Institution for Science
42 protocols

Timo A Lehti University of Helsinki
18 protocols

Zhenying Liu University of Pennsylvania
2 protocols

评审
Molly M. Leung
  • University of British Columbia
研究方向
  • 微生物学
  • 已发表 protocol 3

Education

Ph.D. in Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, 2010

Current position

Post-doctorate in Chemical and Biomolecular Engineering, University of California Los Angeles, Los Angeles, California, USA

Publications

  1. Leung, M. M., Brimacombe, C. A. and Beatty, J. T. (2013). Transcriptional regulation of the Rhodobacter capsulatus response regulator CtrA. Microbiology 159(Pt 1): 96-106.
  2. Leung, M. M., Brimacombe, C. A., Spiegelman, G. B. and Beatty, J. T. (2012). The GtaR protein negatively regulates transcription of the gtaRI operon and modulates gene transfer agent (RcGTA) expression in Rhodobacter capsulatus. Mol Microbiol 83(4): 759-774.
  3. Leung, M. M., Florizone, S. M., Taylor, T. A., Lang, A. S. and Beatty, J. T. (2010). The gene transfer agent of Rhodobacter capsulatus. Adv Exp Med Biol 675: 253-264.
  4. Tuschak, C., Leung, M. M., Beatty, J. T. and Overmann, J. (2005). The puf operon of the purple sulfur bacterium Amoebobacter purpureus: structure, transcription and phylogenetic analysis. Arch Microbiol 183(6): 431-443.
已发表 protocol 3篇
Bacterial Conjugation in Rhodobacter capsulatus
荚膜红细菌中的细菌接合

作者:Molly M. Leung and John Thomas Beatty日期:07/05/2013,浏览量:11402,Q&A: 0
Bacterial conjugation of plasmids is the common method of introducing foreign DNA into Rhodobacter capsulatus because transformational systems have not been shown as efficient methods of introducing DNA to R. capsulatus. For R. ...
Rhodobacter capsulatus Gene Transfer Agent Transduction Assay
荚膜红细菌基因转移因子转导试验

作者:Molly M. Leung and John Thomas Beatty日期:02/20/2013,浏览量:9139,Q&A: 0
The gene transfer agent (GTA) is a bacteriophage-like particle that transfers genomic DNA from a donor to a recipient bacterium. The Rhodobacter capsulatus GTA (RcGTA) was the first to be studied and this protocol has been optimized for ...
参审 protocol 2篇
Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein
SARS-CoV2核衣壳蛋白的异源表达与纯化
作者:Ankur Garg, Lihong Liu, David D. Ho and Leemor Joshua-Tor日期:08/05/2020,浏览量:2908,Q&A: 0
This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, ...
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Generation of Gene Knockout and Gene Replacement with Complete Removal of Full-length Endogenous Transcript Using CRISPR-Trap
通过CRISPR-Trap完全移除内源全长转录本进行基因敲除和基因置换
作者:Jonas Mechtersheimer, Stefan Reber and Marc-David Ruepp日期:10/20/2018,浏览量:5662,Q&A: 0
This protocol describes the application of the CRISPR-Trap from designing of the gene targeting strategy to validation of successfully edited clones that was validated on various human cell lines, among them human induced pluripotent stem cells ...
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