联合作者(
Guillermo Pérez Departement of Medical Sciences, University of Girona, Spain
1 protocol

Fabiana Silvia Scornik University of Girona
2 protocols

Elisabet Selga Cardiovascular Genetics Center & Medical Sciences Department, Girona Biomedical Research Institute (IDIBGI) & University of Girona, Spain
2 protocols

Anna Tarradas Cardiovascular Genetics Center, Girona Biomedical Research Institute (IDIBGI) & University of Girona, Spain
2 protocols

Ramon Brugada
  • Cardiovascular Genetics Center & Medical Sciences Department, Girona Biomedical Research Institute (IDIBGI) & University of Girona School of Medicine, Spain
研究方向
  • 细胞生物学
  • 已发表 protocol 2
已发表 protocol 2篇
Sodium Current Measurements in HEK293 Cells
HEK293细胞中的钠电流测定

作者:Helena Riuró, Anna Tarradas, Elisabet Selga, Ramon Brugada, Fabiana Scornik and Guillermo Pérez日期:08/20/2013,浏览量:12294,Q&A: 0
This protocol is used to measure sodium currents from heterologously transfected cells lines, such as HEK293 cells. Standard whole cell patch clamp technique is used to assess ion channel function. This protocol has been used to study cardiac type Na ...
Cell Surface Protein Biotinylation and Analysis
细胞表面蛋白质的生物素修饰和分析

作者:Anna Tarradas, Elisabet Selga, Helena Riuró, Fabiana S. Scornik, Ramon Brugada and Marcel Vergés日期:08/20/2013,浏览量:27608,Q&A: 0
A great way to specifically isolate and quantify proteins in the cell surface membrane is to take advantage of the biotinylation technique. It consists of labeling cell surface proteins with a biotin reagent before lysing the cells, and isolating ...