联合作者(
Garrett E. Berry Gene Therapy Center, The University of North Carolina at Chapel Hill, USA
1 protocol

Gary R. Whittaker Department of Microbiology and Immunology, Cornell University, Ithaca NY
2 protocols

Yueting Zhang Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, USA
1 protocol
联合评审(
Akira Karasawa New York Structural Biology Center
8 protocols

Arsalan Daudi University of California
157 protocols

Beatrice Li University of Oxford
7 protocols

Christina Yan Ru Tan Agency for Science, Technology and Research (A*STAR)
4 protocols

编委
Longping Victor Tse
  • Post-Doc, University of North Carolina at Chapel Hill
研究方向
  • Gene Therapy
  • Gene Delivery
    Vector Engineering
    Infectious Diseases
  • 已发表 protocol 2

教育背景

PhD, Cornell University, 2014

发表论文

已发表 protocol 2篇
Virus Binding and Internalization Assay for Adeno-associated Virus
腺相关病毒的病毒结合和内化测定实验

作者:Garrett E. Berry and Longping V. Tse日期:01/20/2017,浏览量:9648,Q&A: 0
The binding and internalization of adeno-associated virus (AAV) is an important determinant of viral infectivity and tropism. The ability to dissect these two tightly connected cellular processes would allow better understanding and provide insight ...
Immunoplaque Assay (Influenza Virus)
免疫斑块试验(流感病毒)

作者:Longping V. Tse, Yueting Zhang and Gary R. Whittaker日期:11/05/2013,浏览量:21533,Q&A: 1
Despite developed long time ago, plaque assay is still the gold standard for viral titer quantification in modern virology. The standard crystal violet-based plaque assay relies on virus’ ability to induce cytopathic effect (CPE) which limits the ...
参审 protocol 5篇
Expression and Purification of the Cas10-Csm Complex from Staphylococci
表达并纯化葡萄球菌的Cas10-Csm复合体
作者:Lucy Chou-Zheng and Asma Hatoum-Aslan日期:06/05/2017,浏览量:8345,Q&A: 0
CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) is a class of prokaryotic immune systems that degrade foreign nucleic acids in a sequence-specific manner. These systems rely upon ribonucleoprotein ...
查看全文 >
Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors
通过直接原核显微注射CRISPR/Cas9质粒载体生成突变株
作者:Chin-kai Chuang, Ching-Fu Tu and Chien-Hong Chen日期:06/05/2017,浏览量:8898,Q&A: 0
A set of Cas9 and single guide CRISPR RNA expression vectors was constructed. Only a very simple procedure was needed to prepare specific single-guide RNA expression vectors with high target accuracy. Since the de novo zygotic transcription ...
查看全文 >
主审 protocol 22篇
Environmental Conditioning and Aerosol Infection of Mice
环境调节与小鼠气溶胶感染
作者:Eriko Kudo and Akiko Iwasaki日期:04/20/2020,浏览量:2390,Q&A: 0
Influenza infection models in mice are widely used to study flu-mediated immune responses and pathology. However, most laboratory mice are housed at 20 °C and 50% relative humidity (RH). To better recapitulate influenza epidemics and immune ...
查看全文 >
Expression and Purification of Adeno-associated Virus Virus-like Particles in a Baculovirus System and AAVR Ectodomain Constructs in E. coli
腺相关病毒样颗粒在杆状病毒系统中的表达纯化及在大肠杆菌AAVR胞外区的构建
作者:Nancy Meyer, Omar Davulcu, Qing Xie, Mark Silveria, Grant M. Zane, Edward Large and Michael S. Chapman日期:02/05/2020,浏览量:3585,Q&A: 0
Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape. Presented here are protocols for ...
查看全文 >