联合作者(
Marisa Rosa Bio-protocol.org
2 protocols

Ortrun Mittelsten Scheid
  • Gregor Mendel Institute of Molecular Plant Biology, Austria
研究方向
  • 植物科学
  • 已发表 protocol 2
已发表 protocol 2篇
Measuring Homologous Recombination Frequency in Arabidopsis Seedlings
拟南芥幼苗中同源重组频率的测定

作者:Marisa Rosa and Ortrun Mittelsten Scheid日期:04/05/2014,浏览量:10432,Q&A: 0
Somatic homologous recombination (SHR) is a major pathway of DNA double-strand break (DSB) repair, in which intact homologous regions are used as a template for the removal of lesions. Its frequency in plants is generally low, as most DSB are ...
DNA Damage Sensitivity Assays with Arabidopsis Seedlings
拟南芥幼苗的DNA损伤敏感性实验

作者:Marisa Rosa and Ortrun Mittelsten Scheid日期:04/05/2014,浏览量:9028,Q&A: 0
We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a ...