联合作者(
Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Germany
1 protocol
联合评审(
Ali Asghar Kermani University of Michigan, Ann Arbor
8 protocols

Amriti Rajender Lulla UT MD Anderson Cancer Center
6 protocols

Arvind Kumar Singh Chandel School of Medicine The University of Tokyo
2 protocols

Chang Ho Ho Lee Dankook University
4 protocols

编委
David Paul
  • Post-Doc, MRC Laboratory of Molecular Biology
研究方向
  • 分子生物学
  • Molecular Biology
    Virology
    Membranes
  • 已发表 protocol 1

教育背景

PhD, University of Heidelberg, 2013

实验室信息

McMahon Lab
http://www.endocytosis.org

发表论文

已发表 protocol 1篇
Purification of HCV-remodeled and Control ER Membranes
改型丙肝病毒的纯化和内质网莫的控制

作者:David Paul and Ralf Bartenschlager日期:05/20/2014,浏览量:6650,Q&A: 0
As for all positive strand RNA viruses, hepatitis C virus (HCV) RNA replication is tightly associated with rearranged host cell membranes, termed viral replication factories. However, up to now little is known about both viral and cellular ...
参审 protocol 8篇
In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
真核先导链/后随链DNA复制的体外分析
作者:Grant Schauer, Jeff Finkelstein and Mike O’Donnell日期:09/20/2017,浏览量:7043,Q&A: 0
The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, ...
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An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes
利用PEI优化生产,滴定和中和SARS-CoV spike萤光素酶假病毒的方法
作者:George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton日期:08/20/2017,浏览量:11634,Q&A: 1
The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...
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主审 protocol 33篇
A Multi-color Bicistronic Biosensor to Compare the Translation Dynamics of Different Open Reading Frames at Single-molecule Resolution in Live Cells
一种多色双顺反子生物传感器比较活细胞中不同开放阅读框在单分子分辨率下的翻译动力学
作者:Amanda L. Koch, Tatsuya Morisaki and Timothy J. Stasevich日期:07/20/2021,浏览量:823,Q&A: 0

Here, we describe how to image and quantitate the translation dynamics of a bicistronic biosensor that we recently created to fairly compare cap-dependent and IRES-mediated translation at single-molecule resolution in live human cells. This

...
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Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
基于抗体系标记的简化表观基因组图谱
作者:Steven Henikoff, Jorja G. Henikoff and Kami Ahmad日期:06/05/2021,浏览量:1288,Q&A: 0

We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples

...
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