联合作者(
Jennifer A. Philips Department of Medicine, Division of Infectious Diseases, New York University School of Medicine, USA
1 protocol
联合评审(
Achille Broggi Harvard Medical School
15 protocols

Alexandros C Kokotos Weill Cornell Medicine
18 protocols

Amit Kumar Dey International Centre for Genetic Engineering and Biotechnology
17 protocols

Amriti Rajender Lulla UT MD Anderson Cancer Center
6 protocols

编委
Alka Mehra
  • Research scientist, Institute of Genomics and Integrative Biology
研究方向
  • 微生物学
  • host-pathogen interactions, macrophages, innate immunity, microbiology, cellular trafficking pathways, signaling, microRNAs.
  • 已发表 protocol 2

教育背景

Ph.D., Jawaharlal Nehru University

发表论文

1. Portal-Celhay C, Tufariello JM, Srivastava S, Zahra A, Klevorn T, Grace PS, Mehra A, Park HS, Ernst JD, Jacobs Jr. WR, and Philips JA. (2016) Mycobacterium tuberculosis EsxH inhibits ESCRT-dependent CD4+ T cell activation. Nature Microbiology Dec5 (2) 16232.

2. Mehra A. (2015). Microbial CRISPR-Cas System: From Bacterial Immunity to Next-Generation Antimicrobials. Microbial Factories (Springer India 2015, ISBN No.: 978-81-322-2594-2) Volume 2, Chapter 14. 217-234. DOI: 10.1007/978-81-322-2595-9_14.

3. Mehra A. (2014). Phagolysosomal Trafficking Assay. Bio-protocol 4 (13) e1163; DOI: https://dx.doi.org/10.21769/BioProtoc.1163.

4. Mehra A* and Philips JA*. (2014). Analysis of Mycobacterial Protein Secretion. Bio-protocol 4 (12) e1159; *Co-corresponding author. DOI: https://dx.doi.org/10.21769/BioProtoc.1159.

5. Siegrist MS, Steigedal M, Ahmad R, Mehra A, Dragset MS, Schuster BM, Philips JA, Carr SA, Rubin EJ. (2014). Mycobacterial Esx-3 requires multiple components for iron acquisition. mBio. 5 (3) e01073-14.

6. Mehra A*, Zahra A*, Thompson V, Sirisaengtaksin N, Wells A, Porto M, Köster S, Penberthy K, Kubota Y, Dricot A, Rogan D, Vidal M, Bean AJ, Hill D, Philips JA. (2013). Mycobacterium tuberculosis type VII secreted effector, EsxH targets host ESCRT to inhibit intracellular trafficking. PLOS Pathogens. 9(10) e100373. * Equal contribution.

7. Singh Y, Kaul V, Mehra A, Chatterjee S, Tousif S, Dwivedi VP, Suar M, Kaer LV, Bishai WR and Das G. (2013). Mycobacterium tuberculosis controls miR99b expression in infected murine dendritic cells to modulate host immunity. Journal of Biological Chemistry. Feb 15; 288(7): 5056-61.

8. Juranek J, Kothary P, Mehra A, Hays A, Brannagan III TH, Schmidt AM. (2013). Increased expression of the Receptor for Advanced End-Glycation Products in the neuropathic peripheral nerve of human patients. Brain and Behaviour. 3(6) 701-709.

9. Mehra A, Bhattacharya S and Bhattacharya A. (2007). Identification of a novel lysine rich protein from Entamoeba histolytica. American J Biochemistry and Biotechnology. 3(4): 193-198.

10. Mehra A, Fredrick J, Petri WA Jr, Bhattacharya S and Bhattacharya A. (2006). Expression and function of a family of transmembrane kinases from the protozoan parasite Entamoeba histolytica. Infect and Immun. Sep; 74(9): 5341-51.

11. Arya R, Mehra A, Bhattacharya S, Vishwakarma RA and Bhattacharya A. (2003). Biosynthesis of Entamoeba histolytica Proteophosphoglycan in vitro. Mol. Biochem. Parasitol. Jan, 126 (1): 1-8.
已发表 protocol 2篇
Phagolysosomal Trafficking Assay
吞噬溶酶体的胞内转运分析

作者:Alka Mehra日期:07/05/2014,浏览量:13129,Q&A: 0
Phagolysosomal trafficking is an important innate defense pathway that clears microbes by delivering them to lysosomes, the degradative compartment of the cell. Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, subverts ...
Analysis of Mycobacterial Protein Secretion
分枝杆菌蛋白质分泌物的分析

作者:Alka Mehra and Jennifer A. Philips日期:06/20/2014,浏览量:11321,Q&A: 0
Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis. Analysis of proteins secreted by Mtb has been of interest to the field of tuberculosis research since certain secreted proteins interact with the host to promote ...
参审 protocol 9篇
Isolation of Microglia and Analysis of Protein Expression by Flow Cytometry: Avoiding the Pitfall of Microglia Background Autofluorescence
小胶质细胞的分离及蛋白表达的流式细胞仪分析:避免小胶质细胞背景荧光的陷阱
作者:Jeremy C. Burns, Richard M. Ransohoff and Michaël Mingueneau日期:07/20/2021,浏览量:944,Q&A: 0

Microglia are a unique type of tissue-resident innate immune cell found within the brain, spinal cord, and retina. In the healthy nervous system, their main functions are to defend the tissue against infectious microbes, support neuronal networks

...
查看全文 >
Adoptive Transfer of Monocytes Sorted from Bone Marrow
骨髓源性单核细胞的过继转移
作者:Damya Laoui, Eva Van Overmeire, Chloé Abels, Jiri Keirsse and Jo A Van Ginderachter日期:01/05/2019,浏览量:5122,Q&A: 0
Inflammatory Ly6Chi monocytes can give rise to distinct mononuclear myeloid cells in the tumor microenvironment, such as monocytic myeloid-derived suppressor cells (Mo-MDSC), immature macrophages, M2-like tumor-associated macrophages ...
查看全文 >
主审 protocol 31篇
Primer ID Next-Generation Sequencing for the Analysis of a Broad Spectrum Antiviral Induced Transition Mutations and Errors Rates in a Coronavirus Genome
用于分析冠状病毒基因组中广谱抗病毒药物诱导的过渡突变和错误率的引物ID下一代测序
作者:Shuntai Zhou, Collin S. Hill, Michael U. Clark, Timothy P. Sheahan, Ralph Baric and Ronald Swanstrom日期:03/05/2021,浏览量:3066,Q&A: 0

Next generations sequencing (NGS) has become an important tool in biomedical research. The Primer ID approach combined with the MiSeq platform overcomes the limitation of PCR errors and reveals the true sampling depth of population sequencing,

...
查看全文 >
Ribosome Purification from an α-proteobacterium and rRNA Analysis by Northern Blot
α-变形杆菌核糖体的纯化及Northern印迹分析
作者:Liang Yin and Caroline S. Harwood日期:12/05/2020,浏览量:927,Q&A: 0
Ribosomes are an integral part of cellular life. They are complex molecular machines consisting of multiple ribosomal proteins and RNAs. To study different aspects of ribosome composition, many methods have been developed over the decades. Here, we ...
查看全文 >