联合作者(
Naotaka Nishimura Department of Molecular Genetics, Kumamoto University, Japan
1 protocol
联合评审(
Juan Facundo Rodriguez Ayala Instituto de Nanociencia y Nanotecnología
25 protocols

Toshitsugu Fujita Hirosaki University Graduate School of Medicine
6 protocols

评审
Masato Yano
  • Kumamoto Health Science University
研究方向
  • 细胞生物学
  • 已发表 protocol 1
已发表 protocol 1篇
Separation of the Inner and Outer Mitochondrial Membrane in HeLa Cells
HeLa细胞中线粒体内外膜的分离

作者:Naotaka Nishimura and Masato Yano日期:11/20/2014,浏览量:23173,Q&A: 2
Mitochondria are organelles that have important functions in oxidative phosphorylation, fatty acid oxidation and apoptosis signaling. They have two distinct membranes, outer membrane (OM) and inner membrane (IM). IM contains respiratory chain ...
参审 protocol 2篇
Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli
重组Skd3(人ClpB)蛋白和烟草蚀刻病毒(TEV)蛋白酶在大肠杆菌中的表达与纯化
作者:Ryan R. Cupo and James Shorter日期:12/05/2020,浏览量:1622,Q&A: 0

Skd3 (encoded by human CLPB) is a mitochondrial AAA+ protein comprised of an N-terminal ankyrin-repeat domain and a C-terminal HCLR-clade nucleotide-binding domain. The function of Skd3 has long remained unknown due to challenges in purifying the

...
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Manganese Superoxide Dismutase Activity Assay in the Yeast Saccharomyces cerevisiae
啤酒酵母中的锰超氧化物歧化酶活性测定
作者:Louise Thines and Pierre Morsomme日期:03/05/2020,浏览量:1825,Q&A: 0
Superoxide dismutases (SODs) act as a primary defence against reactive oxygen species (ROS) by converting superoxide anion radicals (O2-) into molecular oxygen (O2) and hydrogen peroxide (H2O2). ...
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