联合作者(
Jeffrey J. DeStefano Department of Cell Biology and Molecular Genetics, University of Maryland, USA
2 protocols
联合评审(
Alessandro Didonna University of California, San Francisco
51 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
50 protocols

Arsalan Daudi University of California
157 protocols

Chhuttan L Meena National Chemical laboratory NCL Pune
2 protocols

评审
Vasudevan Achuthan
  • Post-Doc, Dana Farber Cancer Institute
研究方向
  • 微生物学
  • HIV integration and latency
  • 已发表 protocol 2

教育背景

PhD, University of Maryland, 2016

实验室信息

PI: Alan Engelman

发表论文

1. Achuthan V, Keith BJ, Connolly BA, DeStefano JJ., 2014, Human immunodeficiency virus reverse transcriptase displays dramatically higher fidelity under physiological magnesium conditions in vitro, J.Virol;88(15):8514-27, PMCID: PMC4135932.
2. Achuthan V, DeStefano JJ., 2015, Alternative divalent cations (Zn2+, Co2+, and Mn2+) are not mutagenic at conditions optimal for reverse transcriptase activity, BMC Biochemistry; May 3;16(1):12, PMCID: PMC4472245.
3. Achuthan V, DeStefano JJ., 2015, Primer extension reactions for the PCR-based lacZα complementation fidelity assay, Bio-protocol, 5(12): e1509, PMCID: PMC4972330.
4. Achuthan V, DeStefano JJ., 2015, Mismatched primer extension assays, Bio-protocol, 5(12): e1508, PMCID: PMC4829118.
5. Achuthan V, Singh K, DeStefano JJ., 2017, Physiological Mg2+ Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro, Biochemistry; 2017 Jan 10;56(1):33-46, PMCID: PMC5453313.
已发表 protocol 2篇
Primer Extension Reactions for the PCR- based α- complementation Assay
引物延伸反应用于基于PCR的α-互补试验

作者:Vasudevan Achuthan and Jeffrey J. DeStefano日期:06/20/2015,浏览量:8968,Q&A: 0
The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity ...
Mismatched Primer Extension Assays
错配引物延伸法

作者:Vasudevan Achuthan and Jeffrey J. DeStefano日期:06/20/2015,浏览量:6256,Q&A: 0
Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific ...
参审 protocol 9篇
Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum
恶性疟原虫无性期原位蛋白质棕榈酰化的点击化学成像
作者:Mansoor A. Siddiqui日期:05/05/2021,浏览量:2187,Q&A: 0

Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases

...
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A Sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA while Preserving Cellular DNA
一种在保留细胞DNA的同时定量检测乙型肝炎病毒共价闭合环状(ccc)DNA的灵敏、特异PCR方法
作者:Benno Zehnder, Stephan Urban and Thomas Tu日期:04/20/2021,浏览量:2829,Q&A: 0

Hepatitis B virus (HBV) is the major cause of liver diseases and liver cancer worldwide. After infecting hepatocytes, the virus establishes a stable episome (covalently closed circular DNA, or cccDNA) that serves as the template for all viral

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