联合作者(
Wolfgang Baumeister Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Germany
1 protocol

Jürgen M. Plitzko Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Germany
1 protocol

Julia Mahamid Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Germany
1 protocol

Tim Laugks Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Germany
1 protocol

Benjamin D. Engel
  • Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Germany
研究方向
  • 植物科学
  • 已发表 protocol 1

Education

Ph.D. in Cell Biology, University of California, San Francisco, USA, 2011

Current position

Postdoctoral Fellow, Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany (2011-2015)

Research profiles can be found at:
OCRID: http://orcid.org/0000-0002-0941-4387
Google Scholar: https://scholar.google.com/citations?user=4ytQ9YIAAAAJ

Publications

  1. Schaffer, M., Engel, B. D., Laugks, T., Mahamid, J., Plitzko, J. M. and Baumeister, W. (2015). Cryo-focused ion beam sample preparation for imaging vitreous cells by cryo-electron tomography. Bio-protocol 5(17): e1575.
  2. Engel, B. D., Schaffer, M., Cuellar, L. K., Villa, E., Plitzko, J. M. and Baumeister, W. (2015). Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography. ELife 4: e04889.
  3. Bhogaraju, S., Weber, K., Engel, B. D., Lechtreck, K. F. and Lorentzen, E. (2014). Getting tubulin to the tip of the cilium: one IFT train, many different tubulin cargo-binding sites? Bioessays 36(5): 463-467.
  4. Bhogaraju, S., Engel, B. D. and Lorentzen, E. (2013). Intraflagellar transport complex structure and cargo interactions. Cilia 2(1): 10.
  5. Shih, S. M., Engel, B. D., Kocabas, F., Bilyard, T., Gennerich, A., Marshall, W. F. and Yildiz, A. (2013). Intraflagellar transport drives flagellar surface motility. Elife 2: e00744.
  6. Engel, B. D., Ishikawa, H., Wemmer, K. A., Geimer, S., Wakabayashi, K., Hirono, M., Craige, B., Pazour, G. J., Witman, G. B., Kamiya, R. and Marshall, W. F. (2012). The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function. J Cell Biol 199(1): 151-167.
  7. Rigort, A., Villa, E., Bauerlein, F. J., Engel, B. D. and Plitzko, J. M. (2012). Integrative approaches for cellular cryo-electron tomography: correlative imaging and focused ion beam micromachining. Methods Cell Biol 111: 259-281.
  8. Mizuno, N., Taschner, M., Engel, B. D. and Lorentzen, E. (2012). Structural studies of ciliary components. J Mol Biol 422(2): 163-180.
  9. Engel, B. D., Ishikawa, H., Feldman, J. L., Wilson, C. W., Chuang, P. T., Snedecor, J., Williams, J., Sun, Z. and Marshall, W. F. (2011). A cell-based screen for inhibitors of flagella-driven motility in Chlamydomonas reveals a novel modulator of ciliary length and retrograde actin flow. Cytoskeleton (Hoboken) 68(3): 188-203.
  10. Engel, B. D., Ludington, W. B. and Marshall, W. F. (2009). Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model. J Cell Biol 187(1): 81-89.
  11. Engel, B. D., Lechtreck, K.-F., Sakai, T., Ikebe, M., Witman, G. B. and Marshall, W. F. (2009). Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagella. Methods in cell biology 93: 157-177.
已发表 protocol 1篇
Cryo-focused Ion Beam Sample Preparation for Imaging Vitreous Cells by Cryo-electron Tomography
低温聚焦离子束方法制备样本用于低温电子断层扫描术观察玻璃化细胞

作者:Miroslava Schaffer, Benjamin D. Engel, Tim Laugks, Julia Mahamid, Jürgen M. Plitzko and Wolfgang Baumeister日期:09/05/2015,浏览量:18181,Q&A: 0
Cryo-electron tomography (CET) is a well-established technique for imaging cellular and molecular structures at sub-nanometer resolution. As the method is limited to samples that are thinner than 500 nm, suitable sample preparation is required to ...