联合作者(
Kayleigh Margaret Dodd Institute of Cancer and Genetics, Cardiff University, UK
1 protocol

Andrew Robert Tee
  • Institute of Cancer and Genetics, Cardiff University, UK
研究方向
  • 癌症生物学
  • 已发表 protocol 1
已发表 protocol 1篇
In vitro mTORC1 Kinase Assay for Mammalian Cells Protocol
哺乳动物细胞的体外mTORC1激酶酶活测试法

作者:Kayleigh Margaret Dodd and Andrew Robert Tee日期:06/05/2016,浏览量:10052,Q&A: 1
Historically, mechanistic target of rapamycin (mTOR) was purified from mammalian cells using mild nonionic detergents such as NP-40 and or Triton-X100 that resulted in dissociation of core regulatory components essential for its native kinase ...