联合作者(
Clifford A. Lowell Department of Laboratory Medicine and the Program in Immunology, University of California, USA
1 protocol
联合评审(
Alesssandro Arduini BROAD Institute of Harvard and MIT
2 protocols

Andrea Puhar MIMS and Department of Molecular Biology, Umeå University
51 protocols

Anita Umesh Illumina
4 protocols

Antoine de Morree Stanford university
36 protocols

编委
Meenal Sinha
  • Research scientist, University of California San Francisco
研究方向
  • 免疫学
  • 已发表 protocol 1

教育背景

Ph.D., University of Texas at Houston, 2008

已发表 protocol 1篇
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
采用流式细胞术分选术分离高纯度原代小鼠II型肺泡上皮细胞

作者:Meenal Sinha and Clifford A. Lowell日期:11/20/2016,浏览量:19179,Q&A: 1
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
参审 protocol 18篇
Imaging Thick Lymph Node Tissue Sections
淋巴结组织厚切片的成像技术
作者:Jyh Liang Hor and Scott N. Mueller日期:09/20/2016,浏览量:9867,Q&A: 0
Our protocol describes a simple procedure for imaging thick lymph node sections by 2-photon microscopy. Lymph nodes are sectioned using a vibratome (vibrating microtome) to produce slices of tissue that can then be stained with fluorescently labeled ...
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House Dust Mite Extract and Cytokine Instillation of Mouse Airways and Subsequent Cellular Analysis
对小鼠气道滴注尘螨提取物和细胞因子进行炎症分析
作者:Chris Tibbitt and Jonathan M. Coquet日期:07/20/2016,浏览量:9412,Q&A: 1
Asthma is a complex disease of the airways primarily mediated by T helper 2 cells and innate lymphoid type 2 cells (Licona et al.,2013). Mice do not develop spontaneous asthma and therefore models have been developed for the assessment of ...
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主审 protocol 11篇
Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice
结核分枝杆菌感染小鼠肺和支气管肺泡灌洗样品中髓样细胞的流式细胞仪分析和荧光激活细胞分选
作者:Alissa C. Rothchild, Dat Mai, Alan Aderem and Alan H. Diercks日期:05/20/2020,浏览量:2318,Q&A: 0
Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing ...
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Evaluation of B Cell Proliferation in vivo by EdU Incorporation Assay
通过EdU掺入法评估体内B细胞增殖
作者:Adi Biram and Ziv Shulman日期:05/05/2020,浏览量:2113,Q&A: 0
Generation of antibodies is crucial for establishing enduring protection from invading pathogens, as well as for maintaining homeostasis with commensal bacteria at mucosal surfaces. Chronic exposure to microbiota- and dietary- derived antigens ...
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