联合作者(
Veronica Truniger Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Spain
1 protocol

W. Allen Miller Department of Plant Pathology and Microbiology, Iowa State University, USA
1 protocol

Jelena J. Kraft Department of Plant Pathology and Microbiology, Iowa State University, USA
1 protocol

Raquel N. Sempere Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Spain
1 protocol

Miguel A. Aranda
  • Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Spain
研究方向
  • 微生物学
  • 已发表 protocol 1
已发表 protocol 1篇
Determination of the Secondary Structure of an RNA fragment in Solution: Selective 2`-Hydroxyl Acylation Analyzed by Primer Extension Assay (SHAPE)
溶液中RNA片段二级机构的测定:引物延伸法(SHAPE)分析选择性2-羟基酰化

作者:Manuel Miras, Raquel N. Sempere, Jelena J. Kraft, W. Allen Miller, Miguel A. Aranda and Veronica Truniger日期:01/20/2015,浏览量:11415,Q&A: 0
This protocol describes the methodology for the determination of the secondary structure of an RNA fragment in solution using Selective 2´-Hydroxyl Acylation analyzed by Primer Extension, abbreviation SHAPE. It consists in the very fast chemical ...