联合作者(
Yunde Zhao National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, China
1 protocol

Rongchen Wang National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, China
1 protocol

Yangbin Gao Section of Cell and Developmental Biology, University of California, USA
1 protocol

Tao Zhang
  • National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, China
研究方向
  • 分子生物学
  • 已发表 protocol 1
已发表 protocol 1篇
Production of Guide RNAs in vitro and in vivo for CRISPR Using Ribozymes and RNA Polymerase II Promoters
利用核酶和RNA聚合酶II启动子体内及体外合成CRISPR系统的引导RNA

作者:Tao Zhang, Yangbin Gao, Rongchen Wang and Yunde Zhao日期:02/20/2017,浏览量:13629,Q&A: 0
CRISPR/Cas9-mediated genome editing relies on a guide RNA (gRNA) molecule to generate sequence-specific DNA cleavage, which is a prerequisite for gene editing. Here we establish a method that enables production of gRNAs from any promoters, in any ...