联合作者(
George Diallinas Faculty of Biology, University of Athens, Greece
1 protocol
联合评审(
Ali Asghar Kermani University of Michigan, Ann Arbor
8 protocols

Belen Sanz Complutense University
6 protocols

Elizabeth Libby Harvard Medical School
18 protocols

Emily K Cope Northern Arizona University
13 protocols

编委
Emilia Krypotou
  • Post-Doc, Yale University
研究方向
  • 微生物学
  • Molecular Microbiology, Bacterial pathogens, Fungal transporters
  • 已发表 protocol 1

教育背景

PhD, National and Kapodistrian University of Athens, 2015

实验室信息

Eduardo Groisman lab

发表论文

1. Krypotou E, Kosti V, Amillis S, Myrianthopoulos V, Mikros E, Diallinas G. Modeling, substrate docking, and mutational analysis identify residues essential for the function and specificity of a eukaryotic purine-cytosine NCS1 transporter. J Biol Chem. 2012, 287: 36792-803.
2. Krypotou E, Diallinas G. Transport assays in filamentous fungi: kinetic characterization of the UapC purine transporter of Aspergillus nidulans. Fungal Genet Biol. 2014, 63: 1-8. doi: 10.1016/j.fgb.2013.12.004.
3. Krypotou E, Lambrinidis G, Evangelidis T, Mikros E, Diallinas G. Modelling, substrate docking and mutational analysis identify residues essential for function and specificity of the major fungal purine transporter AzgA. Mol Microbiol. 2014, 93: 129-45. doi: 10.1111/mmi.12646.
4. Krypotou E, Scazzocchio C, Diallinas G. Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine-uric acid transporter and an intrinsically misfolded polypeptide. Fungal Genet Biol. 2015, 75: 56-63. doi: 10.1016/j.fgb.2015.01.009.
5. Krypotou E., Evangelidis T., Bobonis J., Pittis A.A., Gabaldón T., Scazzocchio C., Mikros E., Diallinas G. Origin, diversification and substrate specificity in the family of NCS1/FUR transporters. Mol Microbiol. 2015, 96: 927-50. doi: 10.1111/mmi.12982.
6. Lougiakis N, Gavriil ES, Kairis M, Sioupouli G, Lambrinidis G, Benaki D, Krypotou E, Mikros E, Marakos P, Pouli N, Diallinas G. Design and synthesis of purine analogues as highly specific ligands for FcyB, a ubiquitous fungal nucleobase transporter. Bioorg Med Chem. 2016, 24: 5941-52.
7. Listeria Placental Infection. Vázquez-Boland JA, Krypotou E, Scortti M.
MBio. 2017 Jun 27;8(3). pii: e00949-17. doi: 10.1128/mBio.00949-17.
8. Control of Bacterial Virulence through the Peptide Signature of the Habitat. Krypotou E, Scortti M, Grundström C, Oelker M, Luisi BF, Sauer-Eriksson AE, Vázquez-Boland J.Cell Rep. 2019 Feb 12;26(7):18151827.e5.ndoi:10.1016/j.celrep.2019.01.073.
已发表 protocol 1篇
Transport Assays in Aspergillus nidulans
巢状曲霉转运蛋白动力学分析实验

作者:Emilia Krypotou and George Diallinas日期:11/20/2013,浏览量:7203,Q&A: 0
Transport assays allow the direct kinetic analysis of a specific transporter by measuring apparent Km and Vmax values, and permit the characterization of substrate specificity profiles through competition ...
参审 protocol 11篇
Mouse Adipose Tissue Protein Extraction
小鼠脂肪组织蛋白提取
作者:Yu A. An and Philipp E. Scherer日期:06/05/2020,浏览量:5100,Q&A: 0
As obesity becomes a global epidemic, the metabolism research field is increasingly focusing on studying the physiological and pathological roles of adipose tissues (AT). However, extracting proteins from AT is challenging due to abundant fat ...
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Development of an Efficient Transformation System for Halotolerant Yeast Debaryomyces hansenii CBS767
耐盐汉斯德巴氏酵母菌CBS767的一种高效转化体系的建立
作者:Anu P. Minhas and Dipanwita Biswas日期:09/05/2019,浏览量:2016,Q&A: 0
Debaryomyces hansenii is one of the most osmotolerant and halotolerant yeasts. Further, its association with traditional cheese and meat products imparting special flavors to these products project this yeast with enormous biotechnological ...
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主审 protocol 9篇
Cytoduction and Plasmiduction in Yeast
酵母细胞诱导中的细胞诱导和质粒诱导
作者:Jane E. Dorweiler and Anita L. Manogaran日期:09/05/2021,浏览量:698,Q&A: 0

Cytoduction, and a related technique referred to as plasmiduction, have facilitated substantial advancements in the field of yeast prion biology by providing a streamlined method of transferring prions from one yeast strain to another. Prions are

...
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Synchronized Real-time Measurement of Sec-mediated Protein Translocation
Sec介导的蛋白质易位的同步实时测量
作者:Riti Gupta, Dmitri Toptygin and Christian M. Kaiser日期:08/20/2021,浏览量:626,Q&A: 0

The Sec translocon, consisting of a heterotrimeric transmembrane channel (SecYEG) and an associated ATPase (SecA), catalyzes the export of unfolded proteins from the cytosol in bacteria. Kinetically resolving protein translocation at high resolution

...
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