联合评审(
Achille Broggi Harvard Medical School
15 protocols

Alexandros C Kokotos Weill Cornell Medicine
18 protocols

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

David Paul MRC Laboratory of Molecular Biology
41 protocols

评审
Marco Di Gioia
  • Post-Doc, Boston Children’s Hospital, Harvard Medical School
研究方向
  • 免疫学

教育背景

Ph.D.

参审 protocol 15篇
A Multi-color Bicistronic Biosensor to Compare the Translation Dynamics of Different Open Reading Frames at Single-molecule Resolution in Live Cells
一种多色双顺反子生物传感器比较活细胞中不同开放阅读框在单分子分辨率下的翻译动力学
作者:Amanda L. Koch, Tatsuya Morisaki and Timothy J. Stasevich日期:07/20/2021,浏览量:1121,Q&A: 0

Here, we describe how to image and quantitate the translation dynamics of a bicistronic biosensor that we recently created to fairly compare cap-dependent and IRES-mediated translation at single-molecule resolution in live human cells. This

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Native Co-immunoprecipitation Assay to Identify Interacting Partners of Chromatin-associated Proteins in Mammalian Cells
天然共免疫沉淀法鉴定哺乳动物细胞中染色质相关蛋白的“伙伴蛋白质”
作者:Afzal Husain, Nasim A. Begum, Maki Kobayashi and Tasuku Honjo日期:12/05/2020,浏览量:2378,Q&A: 0

Protein-protein interactions play key roles in nuclear processes including transcription, replication, DNA damage repair, and recombination. Co-immunoprecipitation (Co-IP) followed by western blot or mass spectrometry is an invaluable approach to

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