联合评审(
Agnieszka Zienkiewicz Nicolaus Copernicus University
23 protocols

Ayelign M. Adal The University of British Columbia
4 protocols

Harrie van Erp Rothamsted Research
12 protocols

Marisa Rosa Bio-protocol.org
71 protocols

评审
Lifeng Liu
  • Post-Doc, DOE Joint Genome Institute
研究方向
  • 分子生物学
  • epigenetics, gene regulation
参审 protocol 4篇
PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System
CRISPR/Cas9系统的PCR介导的一日合成向导RNA
作者:Naim Hassan, Farhana Easmin, Keisuke Ekino and Satoshi Harashima日期:07/05/2021,浏览量:1531,Q&A: 0

Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is

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Identification of Intrinsic RNA Binding Specificity of Purified Proteins by in vitro RNA Immunoprecipitation (vitRIP)
体外RNA免疫沉淀(vitRIP)鉴定纯化蛋白的RNA结合特异性
作者:Marisa Müller, Tamas Schauer and Peter B. Becker日期:03/05/2021,浏览量:1538,Q&A: 0

RNA-protein interactions are often mediated by dedicated canonical RNA binding domains. However, interactions through non-canonical domains with unknown specificity are increasingly observed, raising the question how RNA targets are recognized.

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