联合作者(
Toshimitsu Kawate Molecular Medicine, Cornell University, USA, USA,
2 protocols
联合评审(
Alexandros C Kokotos Weill Cornell Medicine
18 protocols

Arif Md. Rashedul Kabir Hokkaido University
4 protocols

Beatrice Li University of Oxford
7 protocols

Carey Y. L. Huh University of California
4 protocols

评审
Akira Karasawa
  • Staff scientist, New York Structural Biology Center
研究方向
  • Membrane protein biochemistry/biophysics
  • 已发表 protocol 2

教育背景

PhD in Biology, Osaka University, 2008

实验室信息

https://www.comppaa.org/

发表论文

https://www.ncbi.nlm.nih.gov/myncbi/1Riz5PBvtfRM9b/bibliography/public/
1. Karasawa, A. and Kawate, T. High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells.
Bio-101: e2943 (2018)

2. Karasawa, A., Michalski, K., Mikhelzon, P., and Kawate, T.
The P2X7 receptor forms a dye-permeable pore independent of its intracellular domain but dependent on membrane lipid composition.
Elife 6, e31186 (2017)

3. Karasawa, A., and Kawate, T.
Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells.
Bio-protocol 7, e2544 (2017).

4. Karasawa, A., and Kawate, T.
Structural basis for subtype-specific inhibition of the P2X7 receptor.
Elife 5, e22153 (2016)

5. Karasawa A., Swier L.J., Stuart M.C., Brouwers J., Helms B., Poolman B.
Physicochemical factors controlling the activity and energy coupling of an ionic strength-gated ATP-binding cassette (ABC) transporter.
J. Biol. Chem. 288, 29862-71 (2013)

6. Gul N., Schuurman-Wolters G., Karasawa A., Poolman B.
Functional characterization of amphipathic a-helix in the osmoregulatory ABC transporter OpuA.
Biochemistry. 51, 5142-52 (2012)

7. Karasawa A., Erkens G.B., Berntsson R.P., Otten R., Schuurman-Wolters G.K., Mulder F.A., Poolman B.
Cystathionine b synthase CBS domains 1 and 2 fulfill different roles in ionic strength-sensing of the ABC transporter OpuA.
J. Biol. Chem. 286, 37280-37291, (2011)

8. Wolters J.C., Berntsson R.P., Gul N., Karasawa A, Thunnissen A.M., Slotboom D.J., Poolman B.
Ligand binding and crystal structures of the substrate-binding domain of the ABC transporter OpuA.
PLoS One. 5: e10361 (2010)

9. Karasawa A, Mitsui K., Matsushita M., Kanazawa H.
Intermolecular cross-linking of monomers in H. pylori Na+/H+ antiporter NhaA at dimer interface inhibits antiporter activity.
Biochem J. 426, 99–108 (2010)

10. Karasawa A., Mitsui K., Matsushita M., Kanazawa H.
Functional Assembly of the Na+/H+ Antiporter of Helicobacter pylori from Partial Fragments in vivo.
Biochemistry. 46, 14272-83 (2007)

11. Karasawa A., Tsuboi Y., Inoue H., Kinoshita R., Nakamura N., Kanazawa H.
Detection of oligomerization and conformational changes in the Na+/H+ antiporter from Helicobacter pylori by fluorescence resonance energy transfer.
J. Biol. Chem. 280, 41900-41911, (2005)
已发表 protocol 2篇
High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells
表达P2X7受体的HEK细胞摄取染料YO-PRO-1的高通量测定

作者:Akira Karasawa and Toshimitsu Kawate日期:07/20/2018,浏览量:5869,Q&A: 0
P2X7 receptors are extracellular ATP-gated ion channels that play broad physiological and pathological roles in animals (Sluyter, 2017). Activation of P2X7 receptors lead to the opening of membrane channels permeable for small cations like Na+ ...
Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells
在Sf9昆虫细胞中表达纯化哺乳动物P2X7受体蛋白

作者:Akira Karasawa and Toshimitsu Kawate日期:09/05/2017,浏览量:9575,Q&A: 0
The P2X7 receptor is an extracellular ATP-gated ion channel found only in eukaryotes (Bartlett et al., 2014). Due to its unique properties among P2X receptors, such as formation of a large conductance pore, the P2X7 receptor has been ...
参审 protocol 8篇
Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts
膜片钳移液管重复使用的快速酶清洗方法:通过消除膜片钳尝试之间的手动移液管更换来提高吞吐量
作者:Corey R. Landry, Mighten C. Yip, Ilya Kolb, William A. Stoy, Mercedes M. Gonzalez and Craig R. Forest日期:07/20/2021,浏览量:1384,Q&A: 0

The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and

...
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Expression and Purification of the Human Cation-chloride Cotransporter KCC1 from HEK293F Cells for Structural Studies
从HEK293F细胞中人K-Cl转运蛋白KCC1的表达和纯化用于结构研究
作者:Si Liu and Jiangtao Guo日期:04/05/2021,浏览量:1241,Q&A: 0

Cation-chloride cotransporters (CCCs) mediate the coupled, electroneutral symport of cations such as Na+ and/or K+ with chloride across membrane. Among CCCs family, K-Cl cotransporters (KCC1-KCC4) extrude intracellular Cl- by the transmembrane K+

...
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