联合作者(
Karolin Luger Howard Hughes Medical Institute at University of Colorado Boulder, USA
2 protocols

Francesca Mattiroli Hubrecht Institute
2 protocols

Yajie Gu
  • Post-Doc, University of California, San Diego
研究方向
  • 生物化学
  • 已发表 protocol 2

Education

Ph.D, Colorado State University, United States, 2018

Current position

Graduate, Biochemistry, colorado state university, United States

Publications

  1. Mattiroli, F., Gu, Y., Yadav, T., Balsbaugh, J. L., Harris, M. R., Findlay, E. S., Liu, Y., Radebaugh, C. A., Stargell, L. A., Ahn, N. G., Whitehouse, I. and Luger, K. (2017). DNA-mediated association of two histone-bound complexes of yeast Chromatin Assembly Factor-1 (CAF-1) drives tetrasome assembly in the wake of DNA replication. Elife 6.
  2. Mattiroli, F., Gu, Y., Balsbaugh, J. L., Ahn, N. G. and Luger, K. (2017). The Cac2 subunit is essential for productive histone binding and nucleosome assembly in CAF-1. Sci Rep 7: 46274.
  3. Muthurajan, U., Mattiroli, F., Bergeron, S., Zhou, K., Gu, Y., Chakravarthy, S., Dyer, P., Irving, T. and Luger, K. (2016). In Vitro Chromatin Assembly: Strategies and Quality Control. Methods Enzymol 573: 3-41.
已发表 protocol 2篇
Measuring Nucleosome Assembly Activity in vitro with the Nucleosome Assembly and Quantification (NAQ) Assay
核小体组装和定量测定法(NAQ)测定体外核小体组装活性

作者:Francesca Mattiroli, Yajie Gu and Karolin Luger日期:02/05/2018,浏览量:5609,Q&A: 0
Nucleosomes organize the eukaryotic genome into chromatin. In cells, nucleosome assembly relies on the activity of histone chaperones, proteins with high binding affinity to histones. At least a subset of histone chaperones promotes histone ...
FRET-based Stoichiometry Measurements of Protein Complexes in vitro
蛋白质复合物体外基于FRET化学计量学测定

作者:Francesca Mattiroli, Yajie Gu and Karolin Luger日期:02/05/2018,浏览量:6651,Q&A: 0
For a complete understanding of biochemical reactions, information on complex stoichiometry is essential. However, measuring stoichiometry is experimentally challenging. Our lab has developed a FRET-based assay to study protein complex stoichiometry ...