联合作者(
Vincent M Rotello University of Massachusetts, USA
2 protocols
联合评审(
Alessandro Didonna University of California, San Francisco
51 protocols

Julie Weidner AstraZeneca
9 protocols

Tanxi Cai Chinese Academy of Sciences
4 protocols

评审
Rubul Mout
  • Post-Doc, Institute for Protein Design, University of Washington
研究方向
  • 生物化学
  • Protein design, protein engineering, gene editing, nanotechnology
  • 已发表 protocol 2

教育背景

PhD, University of Massachusetts, 2017

实验室信息

David Baker lab
https://www.bakerlab.org/

发表论文

已发表 protocol 2篇
A General Method for Intracellular Protein Delivery through ‘E-tag’ Protein Engineering and Arginine Functionalized Gold Nanoparticles
通过'E标签'蛋白质工程和精氨酸功能化金纳米粒子进行细胞内蛋白质递送的通用方法

作者:Rubul Mout and Vincent M. Rotello日期:12/20/2017,浏览量:5906,Q&A: 0
In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using ...
Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
使用精氨酸功能化金纳米粒子进行CRISPR/Cas9-核糖核蛋白的细胞溶质和细胞核递送以用于基因编辑

作者:Rubul Mout and Vincent M. Rotello日期:10/20/2017,浏览量:9783,Q&A: 1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...
参审 protocol 2篇
cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
cDNA免疫PCR:一种新的基于PCR的抗原检测方法
作者:Chathuni Jayathilake, Takuya Terai and Naoto Nemoto日期:12/20/2019,浏览量:3379,Q&A: 0
Immuno-PCR (IPCR) is a powerful method in antigen detection where a PCR-amplifiable DNA reporter is conjugated to a specific antibody or an aptamer for the target molecule. In the development and application of IPCR, successful conjugation of a ...
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Wheat Germ Agglutinin (WGA)-SDS-PAGE: A Novel Method for the Detection of O-GlcNAc-modified Proteins by Lectin Affinity Gel Electrophoresis
小麦种子凝集素(WGA)-SDS-PAGE:一种利用凝集素亲和凝胶电泳对O-乙酰氨基葡萄糖修饰蛋白进行检测的新方法
作者:Ko Fujioka, Yuji Kubota and Mutsuhiro Takekawa日期:12/05/2018,浏览量:5302,Q&A: 0
Diverse cytoplasmic and nuclear proteins dynamically change their molecular functions by O-linked β-N-acetylglucosamine (O-GlcNAc) modification on serine and/or threonine residues. Evaluation of the O-GlcNAcylation level of a specific protein, ...
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