联合作者(
Manuel Victor Borca USDA-ARS Orient Point
2 protocols

Consuelo Carrillo USDA-APHIS Orient Point
1 protocol

Lauro Velazquez-Salinas USDA-ARS Orient Point
1 protocol

Ediane Silva USDA-ARS Orient Point
1 protocol

Douglas Paul Gladue
  • Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA, USA,
研究方向
  • 微生物学
  • 已发表 protocol 2

Education

Ph.D, Stony Brook University, USA, 2007

Current position

Senior Research Scientist, Plum Island Animal Disease Center, Agricultural Research Service, USDA, USA

Publications

  1. https://scholar.google.com/citations?user=csyqsS0AAAAJ&hl=en 
已发表 protocol 2篇
Detection and Quantification of African Swine Fever Virus in MA-104 Cells
非洲猪瘟病毒在MA-104细胞中的检测与定量

Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC

...
CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus
一种大DNA病毒(非洲猪瘟病毒)的CRISPR/Cas基因编辑

作者:Manuel V. Borca, Keith A. Berggren, Elizabeth Ramirez-Medina, Elizabeth A. Vuono and Douglas P. Gladue日期:08/20/2018,浏览量:6166,Q&A: 0
Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA. However, this homologous ...