联合作者(
Martin Jinek 2 protocols

Jonathan Hall 1 protocol

Katja Bargsten 1 protocol

Christian Berk 1 protocol

Gerald Schwank
  • Gerald Schwank
研究方向
  • 分子生物学
  • 已发表 protocol 1
已发表 protocol 1篇
In vitro Generation of CRISPR-Cas9 Complexes with Covalently Bound Repair Templates for Genome Editing in Mammalian Cells
利用共价修复模板体外制备用于哺乳动物细胞中基因组编辑的CRISPR-Cas9复合物

The CRISPR-Cas9 system is a powerful genome-editing tool that promises application for gene editing therapies. The Cas9 nuclease is directed to the DNA by a programmable single guide (sg)RNA, and introduces a site-specific double-stranded break ...