联合作者(
Jianwei Zhu 1 protocol

Yueqing Xie 1 protocol

Hua Jiang 2 protocols

Cedric Cagliero 1 protocol

Huili Lu
  • Faculty, Shanghai Jiao Tong University
研究方向
  • Biotechnology and Bioengineering
  • recombinant proteins, antibody drug, stem cell therapy
  • 已发表 protocol 1

教育背景

Ph.D., Shanghai Jiao Tong University, 2007

发表论文

• Luo M, Zhao M, Cagliero C, Jiang H, Xie Y, Zhu J, Yang H, Zhang M, Zheng Y, Yuan Y, Du Z, Lu H*. A general platform for efficient extracellular expression and purification of Fab from Escherichia coli. Appl Microbiol Biotechnol. 2019 Mar 6. Doi: 10.1007/s00253-019-09745-8
• Zhao M, Luo M, Xie Y, Jiang H, Cagliero C, Li N, Ye H, Wu M, Hao S, Sun T, Yang H, Zhang M, Lin T, Lu H*, Zhu J*. Development of a recombinant human IL-15·sIL-15Rα/Fc superagonist with improved half-life and its antitumor activity alone or in combination with PD-1 blockade in mouse model. Biomedicine & Pharmacotherapy. 2019 Feb 21;112:108677. Doi: 10.1016/j.biopha.2019.108677
• Zhao M, Wang J, Luo M, Luo H, Zhao M, Han L, Zhang M, Yang H, Xie Y, Jiang H, Feng L, Lu H*, Zhu J*. Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35. Appl Microbiol Biotechnol. 2018 May 22; 102(14), 6105-6117. doi: 10.1007/s00253-018-9021-6
• Chen H, Li N, Xie Y, Jiang H, Yang X, Cagliero C, Shi S, Zhu C, Luo H, Chen J, Zhang L, Zhao M, Feng L, Lu H*, Zhu J*. Purification of Inclusion Bodies Using PEG Precipitation under Denaturing Conditions to Produce Recombinant Therapeutic Proteins from Escherichia coli. Appl Microbiol Biotechnol. 2017 Apr 8. 101(13), 5267-5278. DOI: 10.1007/s00253-017-8265-x
• Shi S, Chen H, Jiang H, Xie Y, Zhang L, Li N, Zhu C, Chen J, Luo H, Wang J, Feng L, Lu H*, Zhu J*. A novel self-cleavable tag Zbasic-∆I-CM and its application in the soluble expression of recombinant human interleukin- 15 in Escherichia coli. Appl Microbiol Biotechnol. 2017 Feb;101(3), 1133-1142. DOI: 10.1007/s00253-016-7848-2
• Sun T, Li C, Han L, Jiang H, Xie Y, Zhang B, Qian X, Lu H*, Zhu J*. Functional knockout of FUT8 in Chinese hamster ovary cells using CRISPR/Cas9 to produce a defucosylated antibody. Eng Life Sci. 2015 Sep;15(6): 660–666. Doi: 10.1002/elsc.201400218.
• Lu H, Liu H, Wang J, Shen J, Weng S, Han L, Sun T, Qian L, Wu M, Zhu S, Yu Y, Han W, Zhu J, Moldenhauer A. The Chemokine CXCL9 Exacerbates Chemotherapy-induced Acute Intestinal Damage through Inhibition of Mucosal Restitution. J Cancer Res Clin Oncol. 2015 Jun;141(6):983-92. Doi: 10.1007/s00432-014-1869-y
• Lu H, Zhu S, Qian L, Xiang D, Zhang W, Nie A, Gao J, Wu M, Gao J, Lu B, Yu Y, Han W, Moldenhauer A. Activated expression of the chemokine Mig after chemotherapy contributes to chemotherapy-induced bone marrow suppression and lethal toxicity. Blood 2012; May 24;119(21):4868-77. doi: 10.1182/blood-2011-07-367581
• Qian L, Zhu S, Shen J, Han X, Gao J, Wu M, Lu H*, Han W. Expression and purification of recombinant human Mig in Escherichia coli and its comparison with murine Mig. Protein Expr Purif 2012 Jan; 82(1): 205-211.
已发表 protocol 1篇
A PhoA-STII Based Method for Efficient Extracellular Secretion and Purification of Fab from Escherichia coli
一种基于phoa stii技术的 大肠杆菌中高效分泌表达的fab片段纯化

作者:Ziyan Wang, Yang Gao, Manyu Luo, Cedric Cagliero, Hua Jiang, Yueqing Xie, Jianwei Zhu and Huili Lu日期:09/20/2019,浏览量:3836,Q&A: 0
In comparison with full-length IgGs, antigen binding fragments (Fabs) are smaller in size and do not require the complexed post-translational modification. Therefore, Fab can be cost-effectively produced using an Escherichia coli (E. coli ...